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公开(公告)号：KR100673071B1
公开(公告)日：2007-01-22
申请号：KR1020050096242
申请日：2005-10-12
Applicant: 대구한의대학교산학협력단
IPC: C12Q1/68
CPC classification number: C12Q1/6827 , C12Q1/6809 , C12Q1/6844
Abstract: A method for discriminating genetic identification of Cervus species is provided to supply accurate Cervus species raw material by evaluating relation among species and genetic variation of the Cervus species using a species specific sequencing primer of the Cervus species. The method comprises the steps of: (a) extracting DNA from Cervus sample; (b) preparing a reaction solution of primer consisting of the extracted DNA, sense primer(CCCTAGATCA CGAGCTTAAT) and anti-sense primer(TGTCTGATAA AATTCATTAAATAGC); (c) amplifying the reaction solution through PCR; (d) analyzing the total nucleotide sequence of the amplified DNA; and (e) comparing a gene obtained by performing a pyrosequencing technique on the amplified DNA in combination with sequences: CGATTCATGGG, Cervus nippon Temminck, sequences: CAGTTCATGGG, Cervus elaphus from Newzealand, sequences: CGGTTTATGGG, Cervus canadensis, sequences: CAGTATATGGG, Rangifer tarandus so as to discriminate the Cervus nippon Temminck, Cervus elaphus, Cervus canadensis and Rangifer tarandus and comparing the gene with sequences: CGAGTTCATGGG, Cervus elaphus from Russia, and sequences: CGATCTATGGG, Cervus elaphus from China so as to discriminate the Cervus elaphus from Russia and the Cervus elaphus from China.
Abstract translation: 提供了一种通过使用鹿类物种特异性测序引物评估鹿茸种类与遗传变异关系，提供精确的鹿茸种类原料的方法。 该方法包括以下步骤：（a）从鹿茸样品中提取DNA; （b）制备由提取的DNA，有义引物（CCCTAGATCA CGAGCTTAAT）和反义引物（TGTCTGATAA AATTCATTAAATAGC）组成的引物的反应溶液; （c）通过PCR扩增反应溶液; （d）分析扩增的DNA的总核苷酸序列; 和（e）将通过对扩增的DNA进行焦磷酸测序技术获得的基因与序列组合：CGATTCATGGG，鹿茸Nippon Temminck，序列：CAGTTCATGGG，来自Newzealand的鹿茸，序列：CGGTTTATGGG，加拿大鹿，序列：CAGTATATGGG，Rangifer tarandus 从而区分日本鹿茸Temminck，鹿茸，加拿大鹿茸和Rangifer tarandus，并将该基因与来自俄罗斯的CGAGTTCATGGG，鹿茸和序列：CGATCTATGGG，中国的鹿茸进行比较，从而将鹿茸从俄罗斯和 来自中国的鹿鹿elaphus。
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公开(公告)号：KR100610312B1
公开(公告)日：2006-08-10
申请号：KR1020040063776
申请日：2004-08-13
Applicant: 대구한의대학교산학협력단
IPC: C12Q1/68
Abstract: 본 발명은 파이로시퀀싱(Pyrosequencing)법에 의한 인삼의 종간 유전자를 감별하는 감별 키트에 관한 것으로써, 상세하게는 고려삼과 서양삼을 파이로시퀀싱법에 의하여 유전자형을 분석하고, 인삼의 종간 특이성 시퀀싱 프라이머를 고안하여 파낙스 종(Panax species)의 종간 연관성과 유전적 변이를 평가하기 위한 감별 키트에 관한 것이다. 본 발명의 감별 키트를 통하여 인삼의 종을 판별함으로써 정확한 인삼 원료를 공급할 수 있으며, 인삼의 품질관리 방법으로 활용 할 수 있다.
고려삼, 서양삼, 파이로시퀀싱(Pyrosequencing)법, 감별 키트-
Patent Agency Ranking
公开(公告)号：KR100765412B1
公开(公告)日：2007-10-09
申请号：KR1020050080659
申请日：2005-08-31
Applicant: 대구한의대학교산학협력단
IPC: C12Q1/68
Abstract: 본 발명은 제한효소절편길이다형성 (Restriction Fragment Length Polymorphism; RFLP)법에 의한 녹용의 종간 유전자 감별 키트에 관한 것으로써, 상세하게는 제한효소 절편 길이 다형성 법에 의하여 매화록 (
Cervus
Nippon Temminck), 마록 (
Cervus
elaphus ), 대록 (
Cervus
canadensis ) 및 순록 (
Rangifer
Tarandus )의 유전자형을 분석하고, 녹용의 종간 특이적 제한효소를 이용하여 녹용의 종간 연관성과 유전적 변이를 평가하기 위한 감별 키트에 관한 것이다. 본 발명의 감별 키트를 통하여 녹용의 종을 판별함으로써 정확한 녹용 원료를 공급할 수 있으며, 녹용의 품질관리 방법으로 활용할 수 있다.
녹용, 매화록, 마록, 대록, 순록, 제한효소 절편 길이 다형성, 감별 키트-
公开(公告)号：KR1020060014989A
公开(公告)日：2006-02-16
申请号：KR1020040063776
申请日：2004-08-13
Applicant: 대구한의대학교산학협력단
IPC: C12Q1/68
CPC classification number: C12Q1/6827 , C12Q1/6809 , C12Q1/6844 , C12Q1/6869
Abstract: 본 발명은 파이로시퀀싱(Pyrosequencing)법에 의한 인삼의 종간 유전자를 감별하는 감별 키트에 관한 것으로써, 상세하게는 고려삼과 서양삼을 파이로시퀀싱법에 의하여 유전자형을 분석하고, 인삼의 종간 특이성 시퀀싱 프라이머를 고안하여 파낙스 종(Panax species)의 종간 연관성과 유전적 변이를 평가하기 위한 감별 키트에 관한 것이다. 본 발명의 감별 키트를 통하여 인삼의 종을 판별함으로써 정확한 인삼 원료를 공급할 수 있으며, 인삼의 품질관리 방법으로 활용 할 수 있다.
고려삼, 서양삼, 파이로시퀀싱(Pyrosequencing)법, 감별 키트-
公开(公告)号：KR1020070024973A
公开(公告)日：2007-03-08
申请号：KR1020050080659
申请日：2005-08-31
Applicant: 대구한의대학교산학협력단
IPC: C12Q1/68
CPC classification number: C12Q1/683 , C12Q1/6827
Abstract: A kit for discriminating genetic identification among Cervus species through restriction fragment length polymorphism(RFLP) method is provided to supply accurate Cervus sample and be utilized for managing the quality of the Cervus. The method for discriminating genetic identification among Cervus species comprises the steps of: (a) extracting DNA from a Cervus sample; (b) preparing a reaction solution including the extracted DNA, a sense or an anti-sense primer; (c) amplifying the reaction solution; and (d) performing a RFLP on the amplified DNA to genotype it. The discrimination kit analyzes the genetic variation among Cervus species through the RFLP method including a PCR reagent containing a DNA extract reagent, a sense primer or an anti-sense primer and a restriction enzyme.
Abstract translation: 提供了一种通过限制性片段长度多态性（RFLP）方法鉴别鹿种的遗传鉴定的试剂盒，用于提供精确的鹿鹿样品，并用于管理鹿的品质。 用于区分种子遗传识别的方法包括以下步骤：（a）从鹿茸样品中提取DNA; （b）制备包括提取的DNA，有义或反义引物的反应溶液; （c）扩增反应溶液; 和（d）对扩增的DNA进行RFLP以对其进行基因型分析。 鉴别试剂盒通过包括DNA提取试剂，有义引物或反义引物和限制酶的PCR试剂在内的RFLP方法分析鹿茸种间的遗传变异。
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公开(公告)号：KR100673070B1
公开(公告)日：2007-01-22
申请号：KR1020050080937
申请日：2005-08-31
Applicant: 대구한의대학교산학협력단
IPC: C12Q1/68
CPC classification number: C12Q1/6827 , C12Q1/686 , C12Q2561/113
Abstract: A method for discriminating genetic identification of ginseng species is provided to be able to supply accurate ginseng raw material through determination of the ginseng species, thereby being used for maintaining quality of the ginseng. The method comprises the steps of: (a) extracting DNA from ginseng sample; (b) method for preparing a reaction solution of primer consisting of the extracted DNA, sense primer(CCAAGTTGCA AACCCATGGT) and anti-sense primer(TTTGATTTCC TTGGCGCATT CC), VIC-primer(VIC-5'TTGGGTGGAT CTCGT-3-MGB) where TagMan MGB probe is attached at 3-terminal of the VIC primer, and FAM-primer(FAM-5'-TTGGGTGGCT CTCGT-3'-MGB) where TagMan MGB probe is attached at 3-terminal of the FAM-primer; (c) amplifying the reaction solution through PCR; (d) performing a real time PCR on the amplified DNA in combination and then analyzing it by comparing a DNA of Panax ginseng binding to the VIC primer obtained from the step(b) and having A nucleotide with a DNA of Panax quinquefolia binding to the FAM primer obtained from the step(b) and having C nucleotide through gene analysis shown in Fig. 1 using an SDS 7000 software so as to determine the Panax ginseng and the Panax quinquefolia.
Abstract translation: 提供了一种辨别人参物种遗传鉴定的方法，可以通过测定人参种类来提供准确的人参原料，从而保持人参的质量。 该方法包括以下步骤：（a）从人参样品中提取DNA; （b）制备由提取的DNA，有义引物（CCAAGTTGCA AACCCATGGT）和反义引物（TTTGATTTCC TTGGCGCATT CC），VIC引物（VIC-5'TTGGGTGGAT CTCGT-3-MGB）组成的引物的反应溶液的方法，其中 TagMan MGB探针连接在VIC引物的3个末端，以及FAM引物（FAM-5'-TTGGGTGGCT CTCGT-3-MGB），其中TagMan MGB探针连接在FAM引物的3个末端; （c）通过PCR扩增反应溶液; （d）组合对经扩增的DNA进行实时PCR，然后通过比较人参参与与步骤（b）获得的VIC引物结合的具有A核苷酸的DNA与DNA结合的Panax quinquefolia进行分析 从步骤（b）获得并具有图8所示的通过基因分析的C核苷酸的FAM引物。 1使用SDS 7000软件，以确定人参和Panax quinquefolia。
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公开(公告)号：KR1020070001402A
公开(公告)日：2007-01-04
申请号：KR1020050056867
申请日：2005-06-29
Applicant: 대구한의대학교산학협력단
IPC: C12Q1/68
CPC classification number: C12Q1/683 , C12Q1/6827
Abstract: A kit for genetically discriminating ginseng species by restriction fragment length polymorphism(RFLP) methods is provided to improve reproducibility and accuracy of discrimination, and evaluate relationship and genetic mutations among ginseng species. The kit for genetically discriminating ginseng species by restriction fragment length polymorphism(RFLP) methods comprises a DNA extracting reagent, a PCR reagent containing sense and antisense primers and restriction enzymes, wherein the sense primer has the nucleotide sequence of SEQ ID NO:1 and the antisense primer has the nucleotide sequence of SEQ ID NO:2; and the restriction enzymes include Sau3AI capable of recognizing the nucleotide sequence of SEQ ID NO:3. A method for genetically discriminating ginseng species comprises the steps of: extracting DNA from a ginseng sample; preparing the reaction solution containing extracted DNA and sense and antisense primers; PCR-amplifying the reaction solution; performing the RFLP method with the amplified DNA product.
Abstract translation: 提供了一种通过限制性片段长度多态性（RFLP）方法对人参进行遗传鉴定的试剂盒，以提高鉴别的重现性和准确性，并评估人参物种之间的关系和遗传突变。 通过限制性片段长度多态性（RFLP）方法用于遗传鉴别人参物种的试剂盒包括DNA提取试剂，含有义和反义引物和限制酶的PCR试剂，其中正义引物具有SEQ ID NO：1的核苷酸序列和 反义引物具有SEQ ID NO：2的核苷酸序列; 并且限制酶包括能识别SEQ ID NO：3的核苷酸序列的Sau3AI。 遗传上鉴别人参物种的方法包括以下步骤：从人参中提取DNA; 制备含有提取的DNA和有义和反义引物的反应溶液; PCR扩增反应溶液; 用扩增的DNA产物进行RFLP方法。
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