pinnatifida )의 유리배우체를 이용한 이종교배 양식방법에 관한 것으로, 구체적으로 넓미역의 유리 배우체와 미역의 유리 배우체간의 이종교배를 위한 최적 조건을 확인하고 상기 조건을 이용하여 재배된 넓미역 및 미역 잡종이 넓미역 및 미역 순종과 비교하여 양식기간이 유의적으로 늘어나고, 생물량이 증가하며, 성장률이 빨라지므로, 전복산업을 포함한 다양한 바다양식을 위한 대형 갈조류 자원으로 유용하게 이용될 수 있다.
Abstract:
PURPOSE: A growing method of undariopsis peterseniana and a gametophyte is provided. CONSTITUTION: The sporophyte of undariopsis peterseniana and undaria pinnatifida releases zoospores. The released zoospores are grown to form a gametophyte. The gametophyte of undariopsis peterseniana and undaria pinnatifida is separated into female and male. Each of female and male gametophyte is grown until the diameter reaches 1-5mm. The grown gametophyte is homogenized and cut into 100-300μm. The cut female and male gametophyte are cultivated at a temperature of 5-20°C, a illumination of 5-40 M, and a photeperiod of 10:14(Light(L):Dark(D)) - 14:10(L:D). The grown undaria pinnatifida is cultivated with undariopsis petereniana. Each of male and female is grown until the diameter reaches 2-3mm.
Abstract:
PURPOSE: A undaria peterseniana mass production method by inducing the regeneration and aging of undaria peterseniana free-living gametophytes is provided by securing the optimal condition for the regeneration and aging of the Undaria peterseniana free-living gametophytes. CONSTITUTION: Zoospores are released from an apothecium site of Undaria peterseniana. The released zoospores are cultured, and form gametophytes. Separate the gametophytes formed into female and male gametophytes. Culture the separated female and male gametophytes until the gametophytes reach the respective diameter of 3-10mm. Cut the cultured female and male gametophytes in the respective size of 50-400 micrometers. Culture the excised female and male gametophytes under the cell count of 3-25 cell/ind., the temperature condition of 5-20deg. C , the illumination of 5-40micromolm-2s-1, and the photoperiod of 10:14(Light:Dark)-14:10(L:D). Cross-fertilize the cultivated female and male gametophytes. Culture the gametophytes in a liquid culture medium of PESI(Provasoli`s enriched seawater media). Culture the female and male gametophytes until the gametophytes reach the diameter size of 5-6mm. Excise the female gametophytes in the size of 50-100 micrometers. Excise the male gametophytes in the size of 60-150 micrometers. Culture the female gametophytes under the cell count of 3-8 cell/ind., the temperature condition of 10-20 deg. C, the illumination of 15-30 micromolm-2s-1, and the photoperiod of 10:14(Light:Dark). Culture the male gametophytes under the cell count of 8-12 cell/ind., the temperature condition of 5-20 deg. C, the illumination of 15-25 micromolm-2s-1, and the photoperiod of 14:10(Light:Dark). [Reference numerals] (AA) Matured thallus of Undaria peterseniana; (BB) Rinsing the matured thallus, cut after washing, with sterilized seawater and ABM solution; (CC) Releasing zoospores at 10°C, 20umolm^-2s^-1, and 10:14h(L:D); (DD) 0.1ml zoospore solution; (EE) Separating the zoospores by dilution; (FF) Female gametophyte; (GG) Male gametophyte; (HH) Mass-producing clone gametophyte; (I1,I2) Cutting and regenerating every 20 days at 15°C, 20umolm^-2s^-1, and 10:14h(L:D); (JJ) Mixing the cut female and male gametophyte at the ratio of 1:1; (KK) Forming gamete for at least 15 days at 15°C, 20umolm^-2s^-1, and 14:10h(L:D); (LL) Forming young sporophyte for 40 days at 15°C, 60umolm^-2s^-1, and 14:10h(L:D)
Abstract:
본 발명은 가공식품 중 함유된 파래류의 종 동정을 위한 단일염기다형성 마커 및 이를 이용한 파래의 종 동정 방법 및 동정용 키트에 관한 것으로, 보다 상세하게는 서열번호 1의 단일뉴클레오티드다형성(SNP) 위치인 439번째 염기를 포함하는 8~100개의 연속 염기로 구성되는 파래의 종 동정용 폴리뉴클레오티드 또는 이의 상보적인 폴리뉴클레오티드 및 이를 이용한 파래의 종 동정 방법 및 동정용 키트에 관한 것이다.
Abstract translation:本发明涉及用于鉴定加工食品中所含的乌尔瓦种的单核苷酸多态性标记,以及用于鉴定乌尔瓦物种的方法和使用其的鉴定试剂盒,更具体地说,涉及用于鉴定乌尔瓦种的多核苷酸,其由8至 100个连续的碱基,包括SEQ ID NO:1的单核苷酸多态性(SNP)的位置的第439位碱基或其互补多核苷酸,以及用于鉴定ulva物种的方法和使用其的鉴定试剂盒。
Abstract:
A method for stamping out pirate organisms of gulfweed is provided to achieve stable production of gulfweed and to achieve high quality gulfweed to consumers through eco-friendly removal of pirate organisms. A method for stamping out pirate organisms of gulfweed includes the steps of: collecting seedlings of gulfweed; cultivating the seedlings of gulf weed yielding leaf bodies; and adjusting pH and salt concentration within pH 3-5 and 9-11 by submerging in a salted device.