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    넓미역 유리배우체의 재생 및 성숙 유도를 통한 넓미역 대량 생산 방법
    1.
    发明公开
    넓미역 유리배우체의 재생 및 성숙 유도를 통한 넓미역 대량 생산 방법 有权
    使用再生和成熟诱导生物力学的免疫生物学特性的生物力学方法

    公开(公告)号：KR1020130001479A

    公开(公告)日：2013-01-04

    申请号：KR1020110062259

    申请日：2011-06-27

    Applicant: 대한민국(관리부서:국립수산과학원) ,  목포대학교산학협력단

    Inventor： 황은경 ,  심철홍 ,  하동수 ,  공용근 ,  박찬선

    IPC: A01G33/00 ,  C12N1/12 ,  A01G7/04

    CPC classification number: A01G33/00 ,  A01G7/045 ,  C12N1/12 ,  Y10S47/01 ,  Y10S47/06

    Abstract: PURPOSE: A undaria peterseniana mass production method by inducing the regeneration and aging of undaria peterseniana free-living gametophytes is provided by securing the optimal condition for the regeneration and aging of the Undaria peterseniana free-living gametophytes. CONSTITUTION: Zoospores are released from an apothecium site of Undaria peterseniana. The released zoospores are cultured, and form gametophytes. Separate the gametophytes formed into female and male gametophytes. Culture the separated female and male gametophytes until the gametophytes reach the respective diameter of 3-10mm. Cut the cultured female and male gametophytes in the respective size of 50-400 micrometers. Culture the excised female and male gametophytes under the cell count of 3-25 cell/ind., the temperature condition of 5-20deg. C , the illumination of 5-40micromolm-2s-1, and the photoperiod of 10:14(Light:Dark)-14:10(L:D). Cross-fertilize the cultivated female and male gametophytes. Culture the gametophytes in a liquid culture medium of PESI(Provasoli`s enriched seawater media). Culture the female and male gametophytes until the gametophytes reach the diameter size of 5-6mm. Excise the female gametophytes in the size of 50-100 micrometers. Excise the male gametophytes in the size of 60-150 micrometers. Culture the female gametophytes under the cell count of 3-8 cell/ind., the temperature condition of 10-20 deg. C, the illumination of 15-30 micromolm-2s-1, and the photoperiod of 10:14(Light:Dark). Culture the male gametophytes under the cell count of 8-12 cell/ind., the temperature condition of 5-20 deg. C, the illumination of 15-25 micromolm-2s-1, and the photoperiod of 14:10(Light:Dark). [Reference numerals] (AA) Matured thallus of Undaria peterseniana; (BB) Rinsing the matured thallus, cut after washing, with sterilized seawater and ABM solution; (CC) Releasing zoospores at 10°C, 20umolm^-2s^-1, and 10:14h(L:D); (DD) 0.1ml zoospore solution; (EE) Separating the zoospores by dilution; (FF) Female gametophyte; (GG) Male gametophyte; (HH) Mass-producing clone gametophyte; (I1,I2) Cutting and regenerating every 20 days at 15°C, 20umolm^-2s^-1, and 10:14h(L:D); (JJ) Mixing the cut female and male gametophyte at the ratio of 1:1; (KK) Forming gamete for at least 15 days at 15°C, 20umolm^-2s^-1, and 14:10h(L:D); (LL) Forming young sporophyte for 40 days at 15°C, 60umolm^-2s^-1, and 14:10h(L:D)

    Abstract translation: 目的：通过诱导无花果自由生存配子体的再生和衰老，通过确保无花果种子自由生存配子体的再生和衰老的最佳条件，提供了一种不育种的大量生产方法。 构成：动物孢子从黑腹果蝇的药膳部位释放出来。 释放的游动孢子被培养，形成配子体。 将形成的配子体分成雌雄配子体。 培养分离的雌雄配子，直到配子体达到3-10mm的相应直径。 切割培养的雌雄配子体大小为50-400微米。 培养切除的雌雄配子体细胞计数为3-25细胞/ ind，温度条件为5-20度。 C，5-40微摩尔-2s -1的照射，光照时间为10:14（Light：Dark）-14：10（L：D）。 对栽培的雌性和雄性配子进行交叉施肥。 在PESI（Provasoli富集海水培养基）的液体培养基中培养配子体。 培养雌雄配子，直到配子体直径达到5-6mm。 消除50-100微米尺寸的雌配子体。 消除大小为60-150微米的雄性配子体。 在3-8细胞/ ind的细胞计数下培养雌配子体，温度条件为10-20度。 C，照度为15-30微摩尔-2s -1，光周期为10:14（Light：Dark）。 培养雄性配子体细胞计数为8-12个细胞/ ind，温度条件为5-20度。 C，照射15-25微摩尔-2s -1，光周期为14:10（Light：Dark）。 （AA）成熟Un of属（Undaria peterseniana）; （BB）冲洗成熟的沙门氏菌，洗涤后切除，用灭菌海水和ABM溶液洗涤; （CC）在10℃，20umol ^ -2s ^ -1和10：14h（L：D）释放游动孢子; （DD）0.1ml游动液溶液; （EE）通过稀释分离游动孢子; （FF）雌配子体; （GG）雄配子体; （HH）大量生产克隆配子体; （I1，I2）在15℃，20umol ^ -2s ^ -1和10：14h（L：D）每20天切割和再生; （JJ）以1：1的比例混合切割的雌性和雄性配子体; （KK）在15℃，20umol ^ -2s ^ -1和14：10h（L：D）下成型配子至少15天; （LL）在15℃，60umol ^ -2s ^ -1和14：10h（L：D）形成年轻孢子体40天，

    넓미역 및 미역의 유리배우체에 의한 이종교배 양식방법
    3.
    发明公开
    넓미역 및 미역의 유리배우체에 의한 이종교배 양식방법 有权
    一种利用生物体外生物学和免疫球蛋白之间的免费生物体外培养的混合培养方法

    公开(公告)号：KR1020130055241A

    公开(公告)日：2013-05-28

    申请号：KR1020110120894

    申请日：2011-11-18

    Applicant: 대한민국(관리부서:국립수산과학원) ,  목포대학교산학협력단

    Inventor： 황은경 ,  하동수 ,  공용근 ,  박찬선

    IPC: A01G33/00 ,  A01H1/02 ,  A01K61/00

    CPC classification number: Y02A40/88 ,  A01G33/00 ,  A01H1/02 ,  A01K61/00 ,  Y02P60/64

    Abstract: PURPOSE: A growing method of undariopsis peterseniana and a gametophyte is provided. CONSTITUTION: The sporophyte of undariopsis peterseniana and undaria pinnatifida releases zoospores. The released zoospores are grown to form a gametophyte. The gametophyte of undariopsis peterseniana and undaria pinnatifida is separated into female and male. Each of female and male gametophyte is grown until the diameter reaches 1-5mm. The grown gametophyte is homogenized and cut into 100-300μm. The cut female and male gametophyte are cultivated at a temperature of 5-20°C, a illumination of 5-40 M, and a photeperiod of 10:14(Light(L):Dark(D)) - 14:10(L:D). The grown undaria pinnatifida is cultivated with undariopsis petereniana. Each of male and female is grown until the diameter reaches 2-3mm.

    Abstract translation: 目的：提供一种不断增长的黄芪和配子体的方法。 构成：未知的peterseniana和undaria pinnatifida的孢子体释放出游动孢子。 释放的游动孢子生长形成配子体。 未成熟的peterseniana和undaria pinnatifida的配子体分为女性和男性。 每个雌配子体和雄配子体生长直到直径达到1-5mm。 将成熟的配子体均质化并切成100-300μm。 切割的雌性和雄性配子体培养温度为5-20℃，照度为5-40M，光子浓度为10:14（Light（L）：Dark（D））-14:10（L ：D）。 种植的无性系具有不定形的petereniana。 每个雄性和雌性生长直到直径达到2-3mm。

    감태 유리배우체의 분리 배양 및 성숙 유도를 통한 감태 대량 생산 방법
    8.
    发明公开
    감태 유리배우체의 분리 배양 및 성숙 유도를 통한 감태 대량 생산 방법 有权
    用于分离和再生ECKLONIA CAVA的游戏性和通过成熟感应大量生产ECKLONIA CAVA的方法

    公开(公告)号：KR1020100093323A

    公开(公告)日：2010-08-25

    申请号：KR1020090012452

    申请日：2009-02-16

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 황은경 ,  위미영 ,  김성철 ,  황미숙 ,  백재민 ,  박찬선

    IPC: C12N1/12 ,  A01G33/00

    Abstract: PURPOSE: A method for producing Ecklonia cava is provided to massively produce Eckolnia cava under the optimal culture condition and to protect environment for artificial cultivation. CONSTITUTION: A method for massively producing Ecklonia cava gametophytes comprises: a step of cutting Ecklonia cava apothecium part to induce release of zoospores; a step of geminating released zoospores in a liquid medium to form gametophytes; a step of separating gametophytes to male and female; a step of culturing the female and male gametophytes in a liquid medium; and a step of homogenizing the female and male gametophytes to cut in 100-200 um; and a step of culturing the cut gametophytes at 10-20°C, 5 ~ 40u molm^-2s^-1 of lightness, and 14:10(L:D). The culture medium is PESI(Provasoli's enriched seawater media).

    Abstract translation: 目的：提供Ecklonia Cava的生产方法，在最佳培养条件下大量生产Eckolnia cava，保护人工栽培环境。 构成：大规模生产Ecklonia Cava配子体的方法包括：切割Ecklonia cava apothecium部分以诱发游动孢子释放的步骤; 在液体介质中引发释放的游动孢子以形成配子体的步骤; 将配子体分为男性和女性的步骤; 在液体培养基中培养雌性和雄性配子体的步骤; 将雌配子和雄配子的同质化在100-200 um的步骤; 在10-20℃，5〜40u molm ^ -2s ^ -1的亮度和14:10（L：D）下培养切割的配子体的步骤。 培养基是PESI（Provasoli的富含海水介质）。

    어장조성용 생분해성 로프망 및 그 제조방법
    9.
    发明授权
    어장조성용 생분해성 로프망 및 그 제조방법 有权
    用于制作钓鱼地的生物可分解网格及其制造方法

    公开(公告)号：KR100942258B1

    公开(公告)日：2010-02-17

    申请号：KR1020090010919

    申请日：2009-02-11

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 박성욱 ,  배재현 ,  황은경 ,  하동수 ,  백재민 ,  조성환

    IPC: A01K61/00

    CPC classification number: Y02A40/81 ,  A01G33/00 ,  A01K61/00 ,  A01K75/00

    Abstract: PURPOSE: A bio-decomposable rope net for fishing ground and a manufacturing method thereof are provided, which can realize an optimal rope net for the fishing ground. CONSTITUTION: A manufacturing method of a bio-decomposable rope net for fishing ground comprises a pellet drying step(S1) for drying pellets to less than 0.0005% of moisture through vacuum drying; a melting and extruding step(S2) for extruding pellets and melting and extruding pellets; a filament discharge step(S3) for forming the molten resin into the fiber filament; a filament cooling step(S4) for air-cooling the fiber filament; a filament winding step(S5) for winding the fiber filament on a bobbin; and a rope net manufacturing step(S6) for manufacturing the rope net using a bunch of fiber filaments.

    Abstract translation: 目的：提供一种用于渔场的生物分解绳网及其制造方法，可以实现渔场的最佳绳网。 构成：用于钓鱼的生物分解绳网的制造方法包括：通过真空干燥将颗粒干燥至小于0.0005％的水分的颗粒干燥步骤（S1） 熔融挤出步骤（S2），用于挤出颗粒并熔融和挤出颗粒; 用于将熔融树脂形成到纤维丝中的丝丝排出步骤（S3）; 长丝冷却工序（S4），对纤维丝进行空气冷却; 纤维丝卷绕步骤（S5），用于将纤维丝缠绕在筒管上; 以及用于使用一束纤维丝制造绳网的绳网制造步骤（S6）。

    갈조류 유리배우체의 대량배양 채묘장치
    10.
    发明授权
    갈조류 유리배우체의 대량배양 채묘장치 有权
    大众文化和种植设备，用于免费生活布朗海豹的游泳玩具

    公开(公告)号：KR101594950B1

    公开(公告)日：2016-02-18

    申请号：KR1020150055149

    申请日：2015-04-20

    Applicant: 대한민국(관리부서:국립수산과학원)

    Inventor： 황은경 ,  백재민 ,  황미숙 ,  박은정 ,  유현일 ,  이상용 ,  이순정

    IPC: A01G33/00 ,  A01K61/00 ,  A01K63/06 ,  A01K63/04

    CPC classification number: Y02A40/81 ,  Y02A40/88

    Abstract: 본발명은일정면적의바닥면과측벽을가지고, 상부에삽입홈이형성된일정두께의가로부재와세로부재가연결되어배양구획을형성하는장치프레임, 상기장치프레임의각 구획에삽입되며, 상부가개구되고배양액을수용할수 있는부피를가진배양용기, 상기장치프레임의내부에설치되어배양용기에빛을조사하는광원, 상기배양용기내부에공기를공급하는공기공급장치, 상기배양구획상부의삽입홈에안착되어채묘틀을배양용기내에고정시키는채묘부로이루어진것을특징으로하는갈조류유리배우체의대량배양채묘장치및 이를이용한갈조류유리배우체의대량배양채묘장치를이용한갈조류유리배우체의대량배양방법및 채묘방법을제공함으로써갈조류의채묘시기와독립하여종묘생산및 공급에유리하며, 신품종의개발과육종이쉽고, 채묘를용이하게할 수있다.

    Abstract translation: 本发明提供了一种用于褐藻的自由活体配子体的大量培养和播种装置，以及使用其的棕色海藻的自由活体配子体的大量培养方法和播种方法。 大规模培育播种装置包括：具有预定面积的底壁和侧壁的装置框架，并将具有插入槽的预定厚度的水平构件连接到垂直构件以形成培养部分; 培养容纳在各培养部中的容器，并具有开口和存放培养液的体积; 光源安装在设备框架内以将光辐射到培养容器; 向培养容器供给空气的供气装置; 以及种植单元，其安置在培养部分上部的插入槽中，以将播种框架固定在培养容器内。 本发明在独立于褐藻的播种期的种子和种子的生产和利用方面是有利的，并且使得用户能够容易地开发和种植新的品种，并且容易地进行播种工作。

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