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    데옥시니발레놀과 특이적으로 결합하는 단클론 항체 및 이를 포함하는 데옥시니발레놀 검출용 킷트
    1.
    发明公开
    데옥시니발레놀과 특이적으로 결합하는 단클론 항체 및 이를 포함하는 데옥시니발레놀 검출용 킷트 有权
    单克隆抗体特异性结合到脱氧维纳洛尔和用于检测包含其中的脱氧十一烯酸的试剂盒

    公开(公告)号：KR1020120024239A

    公开(公告)日：2012-03-14

    申请号：KR1020100087030

    申请日：2010-09-06

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 강환구 ,  차상호 ,  정상희 ,  김성희 ,  김현정 ,  최정업

    IPC: C07K19/00 ,  C07K16/14 ,  C12P21/08 ,  G01N33/577

    Abstract: PURPOSE: A monoclonal antibody which specifically binds to deoxynivalenol and a kit for detecting deoxynivalenol are provided to ensure high affinity to deoxynivalenol. CONSTITUTION: A conjugate contains deoxynivalenol, a linker, ovalbumin. The linker help deoxynivalenol to bind with a carrier protein. A monoclonal anbtibody of deposit number KCLRF-BP-00236 is prepared by injecting the conjugate to a mouse as an immunogen. A kit for detecting deoxynivalenol contains the monoclonal antibody. The kit is used for detecting deoxynivalenol in foods or feeds.

    Abstract translation: 目的：提供特异性结合脱氧雪腐镰刀菌烯醇的单克隆抗体和用于检测脱氧雪腐镰刀菌烯醇的试剂盒，以确保对脱氧雪腐镰刀菌烯醇的高亲和力。 构成：缀合物含有脱氧雪腐镰刀菌烯醇，接头，卵清蛋白。 接头帮助脱氧雪腐镰刀菌烯醇与载体蛋白结合。 通过将缀合物注射到作为免疫原的小鼠中来制备保藏号KCLRF-BP-00236的单克隆抗体。 用于检测脱氧雪腐镰刀菌烯醇的试剂盒含有单克隆抗体。 该试剂盒用于检测食品或饲料中的脱氧雪腐镰刀菌烯醇。

    돼지배아줄기세포 및 돼지 미성숙 난자로부터 돼지배아줄기세포를 제조하는 방법
    3.
    发明公开
    돼지배아줄기세포 및 돼지 미성숙 난자로부터 돼지배아줄기세포를 제조하는 방법 有权
    PORCINE EMBRYONIC STEM CELL及其制备PORCINE EMBRYONIC STEM细胞的方法由含有其的PORCINE IMMATOR OOCYTES

    公开(公告)号：KR1020120118676A

    公开(公告)日：2012-10-29

    申请号：KR1020110036195

    申请日：2011-04-19

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 차상호 ,  소병재 ,  나진주 ,  조은영 ,  정수경 ,  김현정 ,  구나연 ,  이희수

    IPC: C12N5/0735 ,  C12N13/00

    Abstract: PURPOSE: A manufacturing method of pig embryonic stem cells from the pig embryonic stem cells and pig immature ovum is provided to have pluripotency which is similar to pig embryonic stem cells originated from blastodermic vesicle. CONSTITUTION: A manufacturing method of pig embryonic stem cells from the pig embryonic stem cells and pig immature ovum comprises the following steps: maturing ovum by cultivating immature ovum of the pig; parthenogenesizing the matured ovum by activating by electrostimulation; cultivating and incubating the activated ovum with blastocyst; and cultivating inner cell mass originated from the incubated blastodermic vesicle and producing the ovum originated embryonic stem cell. The ovum in the second step is the first or the second ovum. The blastocyst cultivation in the third step is completed in a culture medium in which the fetal bovine serum (FBS) is included. The inner cell mass cultivation in the fourth step is completed in the culture medium including LIF, and BFGF or SCF.

    Abstract translation: 目的：提供来自猪胚胎干细胞和未成熟猪的猪胚胎干细胞的制造方法，其具有与源自胚细胞胚泡的猪胚胎干细胞相似的多能性。 构成：猪胚胎干细胞和未成熟猪的胚胎干细胞的制造方法包括以下步骤：通过培养猪的未成熟卵来成熟卵; 通过电刺激使成熟的卵子成孤雌; 用胚泡培养和孵育活化的卵子; 并培养源自培养的胚泡囊泡的内细胞团，并产生卵子来源的胚胎干细胞。 第二步中的卵子是第一个或第二个卵子。 在第三步骤中的胚泡培养在包含胎牛血清（FBS）的培养基中完成。 在包含LIF和BFGF或SCF的培养基中完成第四步的内细胞培养。

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