公开(公告)号：KR101374190B1

公开(公告)日：2014-03-17

申请号：KR1020110036195

申请日：2011-04-19

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 차상호 ,  소병재 ,  나진주 ,  조은영 ,  정수경 ,  김현정 ,  구나연 ,  이희수

IPC: C12N5/0735 ,  C12N13/00

Abstract: 본 발명은 돼지배아줄기세포주, 및 단위생식(parthenogenesis)법을 이용하여 돼지 미성숙 난자로부터 배반포(blastocyst)를 생산하고, 생산된 배반포를 부화시켜 돼지배아줄기세포를 제조하는 방법에 관한 것으로서, 본 발명의 방법으로 돼지배아줄기세포를 보다 효율적으로 제조할 수 있다.

公开(公告)号：KR1020120118676A

公开(公告)日：2012-10-29

申请号：KR1020110036195

申请日：2011-04-19

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 차상호 ,  소병재 ,  나진주 ,  조은영 ,  정수경 ,  김현정 ,  구나연 ,  이희수

IPC: C12N5/0735 ,  C12N13/00

Abstract: PURPOSE: A manufacturing method of pig embryonic stem cells from the pig embryonic stem cells and pig immature ovum is provided to have pluripotency which is similar to pig embryonic stem cells originated from blastodermic vesicle. CONSTITUTION: A manufacturing method of pig embryonic stem cells from the pig embryonic stem cells and pig immature ovum comprises the following steps: maturing ovum by cultivating immature ovum of the pig; parthenogenesizing the matured ovum by activating by electrostimulation; cultivating and incubating the activated ovum with blastocyst; and cultivating inner cell mass originated from the incubated blastodermic vesicle and producing the ovum originated embryonic stem cell. The ovum in the second step is the first or the second ovum. The blastocyst cultivation in the third step is completed in a culture medium in which the fetal bovine serum (FBS) is included. The inner cell mass cultivation in the fourth step is completed in the culture medium including LIF, and BFGF or SCF.

Abstract translation: 目的：提供来自猪胚胎干细胞和未成熟猪的猪胚胎干细胞的制造方法，其具有与源自胚细胞胚泡的猪胚胎干细胞相似的多能性。 构成：猪胚胎干细胞和未成熟猪的胚胎干细胞的制造方法包括以下步骤：通过培养猪的未成熟卵来成熟卵; 通过电刺激使成熟的卵子成孤雌; 用胚泡培养和孵育活化的卵子; 并培养源自培养的胚泡囊泡的内细胞团，并产生卵子来源的胚胎干细胞。 第二步中的卵子是第一个或第二个卵子。 在第三步骤中的胚泡培养在包含胎牛血清（FBS）的培养基中完成。 在包含LIF和BFGF或SCF的培养基中完成第四步的内细胞培养。