Abstract:
PURPOSE: A method for analyzing bacteria community using 16S rDNA-DGGE and real-time PCR is provided to minimize analysis error. CONSTITUTION: A method for analyzing bacteria community using 16S rDNA-DGGE and real-time PCR comprises: a step of performing PCR amplification using 0.25-0.375 mM of dNTP concentration; or a step of performing real-time PCR using bacteria DNA as a template and primers; a step of performing DGGE of PCR products to measure PCR band intensity; and a step of calculating ratio of bacteria species based on the number and intensity of the bands.