公开(公告)号：KR1020110017790A

公开(公告)日：2011-02-22

申请号：KR1020090075443

申请日：2009-08-14

Applicant: 서울대학교산학협력단

Inventor： 가종억 ,  김경덕 ,  황재홍 ,  김유정

IPC: C12N15/55 ,  C12N15/31 ,  C12N15/63 ,  A01P7/04

Abstract: PURPOSE: A fenitrothion hydrolase gene is provided to be used in bioremediation in a fenitrothion-polluted soil. CONSTITUTION: A fenitrothion hydrolase gene has a base sequence encoding amino acid sequence of sequence number 3. A fenitrothion hydrolase protein has an amino acid sequence of sequence number 3. A recombinant vector, pUC19-ORF has the fenitrothion hydrolase gene. A cell transformed by the reacombinant vector is E. coli DH10B/pUC19-ORF. A composition for removing agricultural chemical contains the gene or protein. The agricultural chemical is an organophosphorus insecticide. The organophosphorus insecticide is fenitrothion, parathion, methyl parathion, EPN(O-ehtyl O-p-nitrophenyl phenylphosphonothioate), or malathion.

Abstract translation: 目的：提供灭虫剂水解酶基因，用于杀虫剂污染土壤中的生物修复。 构成：杀虫剂水解酶基因具有编码序列号3的氨基酸序列的碱基序列。杀螟硫磷水解酶蛋白具有序列号3的氨基酸序列。重组载体pUC19-ORF具有杀螟硫磷水解酶基因。 由重组载体转化的细胞是大肠杆菌DH10B / pUC19-ORF。 用于除去农药的组合物含有该基因或蛋白质。 农药是有机磷杀虫剂。 有机磷杀虫剂是杀螟硫磷，对硫磷，甲基对硫磷，EPN（O-对硝基苯基苯基硫代磷酸酯）或马拉硫磷。

公开(公告)号：KR1020110066018A

公开(公告)日：2011-06-16

申请号：KR1020090122747

申请日：2009-12-10

Applicant: 서울대학교산학협력단

Inventor： 가종억 ,  안재형 ,  김유정 ,  정현미 ,  김태성

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/6844 ,  C12Q2527/137 ,  G01N27/447

Abstract: PURPOSE: A method for analyzing bacteria community using 16S rDNA-DGGE and real-time PCR is provided to minimize analysis error. CONSTITUTION: A method for analyzing bacteria community using 16S rDNA-DGGE and real-time PCR comprises: a step of performing PCR amplification using 0.25-0.375 mM of dNTP concentration; or a step of performing real-time PCR using bacteria DNA as a template and primers; a step of performing DGGE of PCR products to measure PCR band intensity; and a step of calculating ratio of bacteria species based on the number and intensity of the bands.

Abstract translation: 目的：提供使用16S rDNA-DGGE和实时PCR分析细菌群落的方法，以尽量减少分析误差。 构成：使用16S rDNA-DGGE和实时PCR分析细菌群落的方法包括：使用0.25-0.375mM dNTP浓度进行PCR扩增的步骤; 或使用细菌DNA作为模板和引物进行实时PCR的步骤; 进行PCR产物DGGE测量PCR带强度的步骤; 以及根据带的数量和强度计算细菌种类的比例的步骤。