公开(公告)号：KR1020110066018A

公开(公告)日：2011-06-16

申请号：KR1020090122747

申请日：2009-12-10

Applicant: 서울대학교산학협력단

Inventor： 가종억 ,  안재형 ,  김유정 ,  정현미 ,  김태성

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/6844 ,  C12Q2527/137 ,  G01N27/447

Abstract: PURPOSE: A method for analyzing bacteria community using 16S rDNA-DGGE and real-time PCR is provided to minimize analysis error. CONSTITUTION: A method for analyzing bacteria community using 16S rDNA-DGGE and real-time PCR comprises: a step of performing PCR amplification using 0.25-0.375 mM of dNTP concentration; or a step of performing real-time PCR using bacteria DNA as a template and primers; a step of performing DGGE of PCR products to measure PCR band intensity; and a step of calculating ratio of bacteria species based on the number and intensity of the bands.

Abstract translation: 目的：提供使用16S rDNA-DGGE和实时PCR分析细菌群落的方法，以尽量减少分析误差。 构成：使用16S rDNA-DGGE和实时PCR分析细菌群落的方法包括：使用0.25-0.375mM dNTP浓度进行PCR扩增的步骤; 或使用细菌DNA作为模板和引物进行实时PCR的步骤; 进行PCR产物DGGE测量PCR带强度的步骤; 以及根据带的数量和强度计算细菌种类的比例的步骤。