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公开(公告)号：KR1020070067578A
公开(公告)日：2007-06-28
申请号：KR1020050129167
申请日：2005-12-24
Applicant: 재단법인서울대학교산학협력재단
CPC classification number: A61L27/32 , A61F2250/0025 , A61F2310/00796 , A61L27/02 , A61L27/04 , A61L27/10 , A61L27/3604 , A61L27/3637
Abstract: A biomaterial made of a thin film of calcium phosphate crystals for inhibiting osteoclast differentiation and a manufacturing method thereof are provided to increase biocompatibility by preventing a lower substrate coated with the calcium phosphate thin film of high roughness from being exposed. The method for manufacturing the biomaterial coated with a calcium phosphate thin film of high surface roughness comprises the steps of: pouring calcium phosphate supersaturated solution on the surface of a solid; firstly reacting the calcium phosphate supersaturated solution on the surface of the solid at 20~45°C for 20~40 minutes; pouring the calcium phosphate supersaturated solution on the surface of the calcium phosphate thin film; and secondly reacting the calcium phosphate supersaturated solution on the surface of the calcium phosphate thin film at 20~45°C for 60~120 minutes.
Abstract translation: 提供了由用于抑制破骨细胞分化的磷酸钙晶体薄膜制成的生物材料及其制造方法，通过防止涂覆有高粗糙度的磷酸钙薄膜的下基材露出来提高生物相容性。 用高表面粗糙度的磷酸钙薄膜涂覆生物材料的方法包括以下步骤：将磷酸钙过饱和溶液倒入固体表面; 首先在20〜45℃下将磷酸钙过饱和溶液在固体表面反应20〜40分钟; 将磷酸钙过饱和溶液倒入磷酸钙薄膜表面; 再次将磷酸钙过饱和溶液在20〜45℃磷酸钙薄膜表面反应60〜120分钟。
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公开(公告)号：KR1020100018867A
公开(公告)日：2010-02-18
申请号：KR1020080077575
申请日：2008-08-07
Applicant: 재단법인서울대학교산학협력재단
Abstract: PURPOSE: A method for establishing human mesenchymal stem cells from umbilical cord blood is provided to easily obtain mesenchymal stem cells which are not easy to obtain and differentiate the mesenchymal stem cells to osteoblasts, chondrocytes, and adipocytes. CONSTITUTION: A human mesenchymal stem cell is derived from human umbilical cord blood. In order for obtaining the mesenchymal stem cell from the umbilical cord blood, the culture medium is different. A culture medium for primary cell culture is a culture medium of forming colony of endothelial stem cells. Mesenchymal stem cells from the primary culture are established by using culture medium for mesenchymal stem cells.
Abstract translation: 目的：提供一种从脐带血建立人间充质干细胞的方法，可以轻松获得不易获得和分化间充质干细胞与成骨细胞，软骨细胞和脂肪细胞的间充质干细胞。 构成：人类间充质干细胞衍生自人脐带血。 为了从脐带血中获得间充质干细胞，培养基不同。 用于初级细胞培养的培养基是形成内皮干细胞集落的培养基。 通过使用间充质干细胞的培养基建立来自原代培养物的间充质干细胞。
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公开(公告)号：KR1020030070713A
公开(公告)日：2003-09-02
申请号：KR1020020010227
申请日：2002-02-26
Applicant: 재단법인서울대학교산학협력재단
IPC: A61L27/30
Abstract: PURPOSE: Provided is a method for forming the calcium phosphate crystal thin film on the solid surface wherein the calcium phosphate crystal thin film is formed in a short time, on the hydrophobic surface which is difficult in forming the crystal thin film. CONSTITUTION: The method for forming the calcium phosphate crystal thin film on the solid surface includes coating with the coatings composed of the organic material which is adhered to the solid material and promotes to form a thin film; contacting it with the supersaturated calcium phosphate solution; and cultivating it to form the calcium phosphate crystal thin film. The coatings composed of the organic material has a crystal structure with the hydrophilic and hydrophobic domains together, acts as a linker that is adhered to the solid surface and links the hydrophobic surface with calcium phosphate, promotes to form a thin film, and does not generate an immune reaction in a human body.
Abstract translation: 目的：提供在形成晶体薄膜的疏水性表面上，在短时间内形成磷酸钙晶体薄膜的固体表面上形成磷酸钙晶体薄膜的方法。 构成：在固体表面上形成磷酸钙晶体薄膜的方法包括用附着在固体材料上的有机材料组成的涂层进行涂覆并促进形成薄膜; 使其与过饱和磷酸钙溶液接触; 并培养其形成磷酸钙晶体薄膜。 由有机材料构成的涂层具有一起具有亲水和疏水畴的晶体结构，作为连接器固定在固体表面上并将疏水表面与磷酸钙连接，促进形成薄膜，不产生 人体免疫反应。
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公开(公告)号：KR1020090005683A
公开(公告)日：2009-01-14
申请号：KR1020070068890
申请日：2007-07-09
Applicant: 재단법인서울대학교산학협력재단
Inventor： 김현만
Abstract: Low soluble amorphous calcium phosphate and a preparation method for the same are provided, which supply calcium phosphate ionic solution of the condition lowering the solubility of the amorphous calcium phosphate. A preparation method for low soluble amorphous calcium phosphate is constituted as follows. The low-melting amorphous calcium phosphate is formed in the solid substrate surface in the following condition. In the pH 6.8-8.0 condition, the calcium phosphate with ion concentration of 2.25-25 mM^2 comes in contact with the solid substrate surface at 0-30 deg.C and the temperature is raised to 8-50 deg.C and the high solubility amorphous calcium phosphate granule is formed in the solid substrate surface.
Abstract translation: 提供了低溶性无定形磷酸钙及其制备方法，其为磷酸钙离子溶液提供降低无定形磷酸钙的溶解度的条件。 低可溶性无定形磷酸钙的制备方法如下构成。 在以下条件下，在固体基材表面形成低熔点无定形磷酸钙。 在pH 6.8-8.0的条件下，离子浓度为2.25-25mM ^ 2的磷酸钙在0-30℃与固体基质表面接触，温度升至8-50℃， 在固体基材表面形成高溶解度无定形磷酸钙颗粒。
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公开(公告)号：KR1020030070859A
公开(公告)日：2003-09-02
申请号：KR1020030012112
申请日：2003-02-26
Applicant: 재단법인서울대학교산학협력재단
IPC: C01B25/32
CPC classification number: B01J20/048 , C01B25/32
Abstract: PURPOSE: A preparation method of calcium phosphate ion solutions, calcium phosphate colloid ion solutions formed by maintaining the calcium phosphate ion solutions at low temperature, dots or thin films of calcium phosphate formed on a solid substrate using the calcium phosphate colloid ion solutions, and calcium phosphate granules prepared in the calcium phosphate colloid ion solutions are provided. CONSTITUTION: The preparation method of calcium phosphate ion solutions comprises a step (i) of preparing an ion solution having pH of 6.0 to 10.0 including phosphoric acid ions; a step (ii) of preparing an ion solution having pH of 6.0 to 10.0 including calcium ions; and a step (iii) of preparing calcium phosphate solution having pH of 6.0 to 10.0 by mixing the ion solutions. The preparation method of calcium phosphate ion solutions comprises a step (i) of preparing an ion solution having pH of 6.0 or less including phosphoric acid ions and calcium ions; and a step (ii) of preparing calcium phosphate solution having pH of 6.0 to 10.0 by mixing a basic solution with the ion solution. The material including dots is characterized in that it is formed by a method of comprising a step (i) of preparing calcium phosphate ion solution prepared by a preparation method comprising a process (a) of preparing calcium ion and phosphoric acid ion solutions by dissolving calcium salt and phosphoric acid salt into an acidic solution, a process (b) of mixing the prepared calcium ion and phosphoric acid ion solutions with an alkali solution at low temperature, and a process (c) of removing amorphous calcium phosphate granules or crystals from the prepared solution; a step (ii) of preparing calcium phosphate colloid ion solution in the calcium phosphate ion solution by leaving alone the prepared calcium phosphate ion solution at a temperature of 0 to 60 deg.C; and a step (iii) of pouring the prepared calcium phosphate colloid ion solution onto a solid substrate.
Abstract translation: 目的：磷酸钙离子溶液的制备方法，通过使用磷酸钙胶体离子溶液在固体底物上形成的磷酸钙离子溶液在低温下保持形成的磷酸钙胶体离子溶液，磷酸钙的点或薄膜，钙 提供了在磷酸钙胶体离子溶液中制备的磷酸盐颗粒。 构成：磷酸钙离子溶液的制备方法包括：（i）制备pH为6.0〜10.0的离子溶液，包括磷酸离子; 制备pH6.0〜10.0的包含钙离子的离子溶液的工序（ii） 以及通过混合离子溶液制备pH为6.0至10.0的磷酸钙溶液的步骤（iii）。 磷酸钙离子溶液的制备方法包括：（i）制备pH6.0或更低的离子溶液，其包括磷酸离子和钙离子; 以及通过将碱性溶液与离子溶液混合来制备pH为6.0至10.0的磷酸钙溶液的步骤（ii）。 包括点的材料的特征在于其形成方法包括：制备磷酸钙离子溶液的步骤（i）的方法，所述方法包括制备方法，所述制备方法包括通过溶解钙来制备钙离子和磷酸离子溶液的方法（a） 盐和磷酸盐转化成酸性溶液，将制备的钙离子和磷酸离子溶液与低温碱溶液混合的方法（b）和（c）从无机磷酸钙颗粒或晶体中除去 制备溶液; 在磷酸钙离子溶液中通过在0〜60℃的温度下离心制备磷酸钙离子溶液来制备磷酸钙胶体离子溶液的步骤（ii）; 和将所制备的磷酸钙胶体离子溶液倒入固体基质上的步骤（iii）。
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Patent Agency Ranking
公开(公告)号：KR100930279B1
公开(公告)日：2009-12-09
申请号：KR1020070060645
申请日：2007-06-20
Applicant: 재단법인서울대학교산학협력재단
Inventor： 김현만
CPC classification number: A61L27/32 , C23C18/1208 , C23C18/1225 , C23C30/00
Abstract: 본 발명은 고체기재 표면에 형성시킨 인산칼슘 결정으로 구성된 두께 200 nm 이하의 극초박막 및 그 제조 방법에 관한 것이다. 인산칼슘 극초박막은 고체기재 표면에 구현된 요철을 우수하게 보전한 상태에서 고체기재 표면을 인산칼슘 결정으로 코팅할 수 있는 효과가 있다. 이 방법은 저온에서 인산칼슘 이온용액을 사용하여 인산칼슘 극초박막을 형성시키는데, 인산칼슘 이온용액을 (1)고체기재 표면에 비정질 인산칼슘 과립을 형성하는 단계, (2)비정질 인산칼슘 과립 주위로 인산칼슘 표면결정의 모체를 형성시키는 단계, (3)비정질 인산칼슘 과립을 용해시켜 제거하는 단계, (4)인산칼슘 표면결정의 모체를 결정으로 변환 및 증식시키는 단계로 각각 구분하여 적용하므로서 인산칼슘 결정의 증식 및 성숙을 제한하여 두께 200 nm 이하의 인산칼슘 결정 극초박막을 제조할 수 있다.
인산칼슘, 극초박막, 비정질 인산칼슘과립, 표면결정의 모체, 결정형성Abstract translation: 公开了一种在固体基材表面上具有200nm或更小厚度的磷酸钙晶体的超薄膜及其制备方法。 此外，公开了用于制备磷酸钙超薄膜的中间材料的非晶树枝状延伸部分及其形成方法。 磷酸钙晶体的超薄膜可以涂布在固体基材的表面上，同时基本保持固体基材的表面粗糙度。 该方法在低温下使用磷酸钙离子溶液。 将离子溶液分开施加到以下步骤：1）在固体基质表面上形成无定形磷酸钙颗粒，2）在无定形磷酸钙颗粒周围形成表面晶体的树枝状前体基质，3）除去无定形颗粒 在无定形颗粒结晶之前通过溶解，以及4）使表面晶体的树枝状前体基质结晶以形成表面晶体，并将该晶体在固体基材的表面上扩展，由此形成厚度为200的磷酸钙晶体的超薄膜 nm或更小。
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公开(公告)号：KR1020090012431A
公开(公告)日：2009-02-04
申请号：KR1020070076241
申请日：2007-07-30
Applicant: 재단법인서울대학교산학협력재단
Inventor： 김현만
Abstract: A method of manufacturing material including a cell adhesive protein spot surface manufactured by using amorphous calcium phosphate granule is provided to implement nano spot of the cell adhesion molecule or a nano network surface. A method of Manufacturing material including a cell adhesive protein spot surface manufactured by using amorphous calcium phosphate granule comprises steps of: contacting a calcium phosphate ionic solution of which calcium phosphate ion concentration is 2.25 ~ 25 mM^2 in a solid substrate surface in pH 6.8 ~ 8.0 condition; contacting the calcium phosphate ionic solution at 0 ~ 30°C and raising temperature to 8 ~ 50°C; and forming amorphous calcium phosphate granule of a diameter 50 ~ 400nm size at the solid substrate surface.
Abstract translation: 提供一种制造包含通过使用无定形磷酸钙颗粒制造的细胞粘附蛋白质斑点表面的材料的方法来实现细胞粘附分子或纳米网络表面的纳米斑点。 使用无定形磷酸钙颗粒制造的包括细胞粘附蛋白质斑点表面的制造材料的方法包括以下步骤：将磷酸钙离子浓度为2.25〜25mM ^ 2的磷酸钙离子溶液与pH 6.8的固体底物表面接触 〜8.0条件; 使磷酸钙离子溶液在0〜30℃下升温至8〜50℃; 并在固体基材表面形成直径为50〜400nm的无定形磷酸钙颗粒。
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公开(公告)号：KR1020080111993A
公开(公告)日：2008-12-24
申请号：KR1020070060645
申请日：2007-06-20
Applicant: 재단법인서울대학교산학협력재단
Inventor： 김현만
CPC classification number: A61L27/32 , C23C18/1208 , C23C18/1225 , C23C30/00
Abstract: A calcium phosphate super-thin film and a manufacturing process thereof are provided, which excellently preserves the concavo-convex of the solid substrate surface. A method for manufacturing calcium phosphate super-thin film comprises: a step for forming the dendrite amorphous protrusion as the parent of the surface crystallization on solid substrate surface from the amorphous calcium phosphate granular surface; a step for dissolving and removing the amorphous calcium phosphate granule after leaving the parent of the surface crystallization adhered to the solid substrate surface; a step for forming the super-thin made of calcium phosphate crystallization having a thickness of 200 nm or less by changing the parent of the surface crystallization into crystallization and multiplying it.
Abstract translation: 提供了一种磷酸钙超薄膜及其制造方法，其极好地保持了固体基材表面的凹凸。 一种制造磷酸钙超薄膜的方法包括：从无定形磷酸钙颗粒表面形成作为在固体基材表面上的表面结晶的母体的枝晶非晶形突起的步骤; 在离开表面结晶的母体附着在固体基材表面上之后溶解和除去无定形磷酸钙颗粒的步骤; 通过将表面结晶的母体改变为结晶并将其倍增，形成厚度为200nm以下的磷酸钙结晶超薄的步骤。
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公开(公告)号：KR100944942B1
公开(公告)日：2010-03-03
申请号：KR1020070068890
申请日：2007-07-09
Applicant: 재단법인서울대학교산학협력재단
Inventor： 김현만
Abstract: 본 발명은 고체기재 표면에 형성시킨 저용해성 비정질인산칼슘(amorphous calcium phosphate) 및 그 제조 방법에 관한 것이다. 용해도가 낮은 비정질인산칼슘은 고용해성 비정질인산칼슘을 모체로 얻을 수 있다. 고용해성 비정질인산칼슘은 인산칼슘 과포화 이온용액으로 저온에서 처리하면 저용해성 비정질인산칼슘으로 물리적 성질이 바뀐다. 용해도가 낮은 비정질인산칼슘은 생체재료로서 유용하게 사용할 수 있다. 소수성 표면에 코팅하여 세포에 친수성 표면을 제공하거나, 서서히 용해하여 칼슘, 인산, 혹은 비정질인산칼슘내에 미리 함유시킨 내용물을 유리하여 세포활성을 조절할 수 있는 효과가 있다.
비정질인산칼슘, 용해도, 생체재료-
公开(公告)号：KR100511338B1
公开(公告)日：2005-08-31
申请号：KR1020030012112
申请日：2003-02-26
Applicant: 재단법인서울대학교산학협력재단
IPC: C01B25/32
CPC classification number: B01J20/048 , C01B25/32
Abstract: 본 발명은 저온에서 안정화된 인산칼슘 이온 용액을 제조하는방법 및 상기 인산칼슘 이온 용액을 이용하여 제조된 인산칼슘 콜로이드에 관한 것이다. 상기 인산칼슘 콜로이드는 고체기재 상에 부착하여 성장시킴으로써
인산칼슘 반점, 섬 또는 박막 형태를 제조할 수 있고, 용액 내에서 성장시킴으로서 인산칼슘 과립을 제조할 수 있다.
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