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公开(公告)号：KR101440153B1
公开(公告)日：2014-09-15
申请号：KR1020070044890
申请日：2007-05-09
Applicant: 재단법인서울대학교산학협력재단
IPC: C12Q1/00
CPC classification number: C12Q1/485 , C07K1/047 , G01N2333/9121
Abstract: 본 발명은 인산화 효소의 기질특이성 분석 방법에 관한 것이다. 보다 상세하게는, 본 발명은 ⅰ) 하나의 지지체 수지 표면에 아미노산 서열이 서로 동일한 펩타이드들을 다수 개 생성시켜 펩타이드 라이브러리를 제조하는 단계; ⅱ) 아미노산 서열을 달리하면서 ⅰ)단계를 수차례 반복하여 각각의 지지체 수지별로는 아미노산 서열이 서로 상이한, 무작위 펩타이드 라이브러리들의 혼성체를 형성하는 단계; ⅲ) 상기 펩타이드 라이브러리들의 혼성체에 인산화 효소를 반응시켜 인산화된 펩타이드 라이브러리를 제조하는 단계; ⅳ) 포스파타아제 및 퍼옥시다아제 중의 어느 하나가 결합되어 있고 인산화된 아미노산에만 선택적으로 결합하는 항체를 상기 인산화된 펩타이드 라이브러리에 반응시키고, 이 반응 혼합물에 선택적으로 작용하는 기질을 가하는 단계; ⅴ) 상기 포스파타아제 및 퍼옥시다아제 중의 어느 하나와 상기 기질의 반응에 의하여 나타나는 물성의 변화를 감지하여 물성의 변화를 일으킨 펩타이드 라이브러리가 존재하는 고체상 지지체 수지만을 선별해 내는 단계; ⅵ) 상기 분리된 고체상 지지체 수지에서 펩타이드를 분리시키고, 이렇게 분리된 펩타이드의 아미노산 서열을 분석하는 단계를 포함하는, 인산화 효소의 기질특이성 분석 방법에 대한 것이다.
인산화 효소-
公开(公告)号：KR1020070003463A
公开(公告)日：2007-01-05
申请号：KR1020050059458
申请日：2005-07-02
Applicant: 재단법인서울대학교산학협력재단
IPC: C07H19/10
Abstract: Peptide nucleic acid(PNA) monomers having a photolabile protecting group are provided to improve preparation speed and economical efficiency of PNA through photolithograph method. The peptide nucleic acid monomers represented by the formulas(I) to (IV) are provided, wherein P is the photolabile protecting group; An is 4-methoxybenzoyl group; iBu is isobutyryl group; and the photolabile protecting group is selected from 2-nitrobenzyl derivative, 2-methyl-2-(2-nitrophenyl)propyloxycarbonyl derivative, benzoin derivative and ortho-nitrobenzyloxy derivative or further includes the compound represented by the formula(V). A method for preparing the peptide nucleic acids represented by the formula(XV) comprises the steps of: (i) reacting the compound represented by the formula(XIII) with the photolabile protecting group so as to prepare the compound represented by the formula(XIV); and (ii) independently reacting the compound represented by the formula(XIV) with 1-(carboxymethyl)-4-N-(4-methoxybenzoyl)cytosine, 9-(carboxymethyl)-2-N-(isobutyryl) guanine, 1-N-carboxymethylthymine and 9-(carboxymethyl)-6-N-(4-methoxybenzoyl)adenine.
Abstract translation: 提供具有光不稳定保护基的肽核酸（PNA）单体，通过光刻法提高PNA的制备速度和经济性。 提供由式（I）至（IV）表示的肽核酸单体，其中P是光不稳定保护基; An是4-甲氧基苯甲酰基; iBu是异丁酰基; 并且光不稳定保护基选自2-硝基苄基衍生物，2-甲基-2-（2-硝基苯基）丙氧基羰基衍生物，苯偶姻衍生物和邻硝基苄氧基衍生物，或还包括由式（V）表示的化合物。 制备由式（ⅩⅤ）表示的肽核酸的方法包括以下步骤：（ⅰ）使由式（ⅩⅢ）代表的化合物与光不稳定保护基反应，以制备由式（ⅩⅣ）表示的化合物 ）; 和（ii）使式（XIV）表示的化合物与1-（羧甲基）-4-N-（4-甲氧基苯甲酰基）胞嘧啶，9-（羧甲基）-2-N-（异丁酰基）鸟嘌呤， N-羧甲基胸腺嘧啶和9-（羧甲基）-6-N-（4-甲氧基苯甲酰基）腺嘌呤。
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公开(公告)号：KR100671286B1
公开(公告)日：2007-01-19
申请号：KR1020050106505
申请日：2005-11-08
Applicant: 재단법인서울대학교산학협력재단
IPC: C12Q1/68
CPC classification number: B01L9/527 , B01L3/5027 , C12Q1/6837
Abstract: A single crystalline silicon micro-mirror is provided to have an optically flat mirror plate, show little deformation of a mechanical spring, and have uniform performance of each micro-mirror and relatively long life span, thereby being adequate for preparing a bio-pattern array when synthesizing peptides. The single crystalline silicon micro-mirror used for bio-pattern fabrication of peptide synthesis comprises a first substrate which is made of single crystalline silicon(10) and includes a first binding portion(13) and a second binding portion(15), each being electrically connected to a first electrode pad and a second electrode pad, and a mirror plate(11) which is electrically connected to the first binding portion through a mechanical spring(12) and rotates using the mechanical spring as a shaft and a second substrate which is made of insulating materials such as glass(20) and electrically separated from the mirror plate with being spaced apart from the lower portion of the mirror plate and includes a bottom electrode(21) electrically connected to the second binding portion and made of metal. The first binding portion and the second binding portion are electrically separated by an insulation groove(14) formed for exposing the second substrate.
Abstract translation: 提供单晶硅微反射镜以具有光学平面镜板，几乎不显示机械弹簧的变形，并且具有每个微镜的均匀性能和相对长的寿命，从而足以制备生物图案阵列 当合成肽时。 用于肽合成生物图案制造的单晶硅微镜包括由单晶硅（10）制成并包括第一结合部分（13）和第二结合部分（15）的第一基底 电连接到第一电极焊盘和第二电极焊盘;以及镜板（11），其通过机械弹簧（12）电连接到第一装订部分并且使用机械弹簧作为轴进行旋转，第二基板 由诸如玻璃（20）的绝缘材料制成并且与镜板电隔离并且与镜板的下部分隔开并且包括电连接到第二结合部分并由金属制成的底部电极（21）。 第一装订部分和第二装订部分由形成用于暴露第二基板的绝缘槽（14）电隔离。
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公开(公告)号：KR1020080099400A
公开(公告)日：2008-11-13
申请号：KR1020070044890
申请日：2007-05-09
Applicant: 재단법인서울대학교산학협력재단
IPC: C12Q1/00
CPC classification number: C12Q1/485 , C07K1/047 , G01N2333/9121
Abstract: An analytical method of analyzing a substrate specificity of a protein phosphatase is provided to determine rapidly the substrate specificity of the protein phosphatase using a peptide library and to apply an analytical method to tyrosine kinase, serine and threonine phosphorylase. An analytical method of analyzing a substrate specificity of a protein phosphatase comprises steps of: i) creating a plurality of peptides at one surface of the supporter resin and manufacturing the peptide library; ii) forming a hybrid of randomizing peptide libraries; iii) reacting phosphorylase in the hybrid of peptide libraries and manufacturing the peptide library which becomes phosphorylation by making specific amino acid of the peptide having the fixed amino acid sequence selectively into a phosphoric acid; iv) reacting an antibody which selectively combines the amino acid becoming the phosphorylation in the peptide library which becoming the phosphorylation and adding a substrate which selectively acts on the reacted mixture; v) selecting only a solid support resin in which the peptide library causing the change of the property exists; and vi) separating the peptide from the separated solid support resin and analyzing an amino acid sequence of the separated peptide.
Abstract translation: 提供分析蛋白质磷酸酶底物特异性的分析方法，以便使用肽文库快速测定蛋白质磷酸酶的底物特异性，并对酪氨酸激酶，丝氨酸和苏氨酸磷酸化酶应用分析方法。 分析蛋白质磷酸酶的底物特异性的分析方法包括以下步骤：i）在载体树脂的一个表面上产生多个肽并制备肽文库; ii）形成随机多肽文库的杂交体; iii）使肽文库的杂交体中的磷酸化酶反应，并通过使具有固定氨基酸序列的肽的特异性氨基酸选择性地制成磷酸形成磷酸化的肽文库; iv）使成为磷酸化的肽文库中选择性结合成为磷酸化的氨基酸的抗体反应，并加入选择性地作用在反应混合物上的底物; v）仅选择导致该性质改变的肽文库的固体支持树脂; 和vi）从分离的固体支持树脂中分离肽并分析分离的肽的氨基酸序列。
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