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公开(公告)号：KR1020040075252A
公开(公告)日：2004-08-27
申请号：KR1020030010772
申请日：2003-02-20
Applicant: 재단법인서울대학교산학협력재단
IPC: C07K14/415
CPC classification number: C12N15/827 , C07K14/415
Abstract: PURPOSE: A gene controlling the flowering time of plants and a method for manipulating the flowering time of plants using the same gene are provided, which gene is useful for improving properties associated with the flowering time in plants and screening the flowering time-controlling gene in other plants. CONSTITUTION: The gene LOV1 controlling the flowering time of plants comprises the nucleotide sequence set forth in SEQ ID NO:1 and has the nucleotide sequence set forth in SEQ ID NO:3, wherein the gene controlling the flowering time of plants encodes a protein LOV1 controlling the flowering time of plants having the amino acid sequence set forth in SEQ ID NO:2 which is isolated from Arabidopsis thaliana; and the gene inhibits the flowering stimulating gene AGL20. The method for manipulating flowering time of plants comprises over-expressing the gene LOV1 for delaying the flowering time or inhibiting the expression of the gene LOV1 for stimulating the flowering time.
Abstract translation: 目的：提供控制植物开花时间的基因和使用相同基因操作植物开花时间的方法，该基因可用于改善与植物开花时间相关的性质，并筛选开花时间控制基因 其他植物。 构成：控制植物开花时间的基因LOV1包含SEQ ID NO：1所示的核苷酸序列，并具有SEQ ID NO：3所示的核苷酸序列，其中控制植物开花时间的基因编码蛋白LOV1 控制具有从拟南芥分离的SEQ ID NO：2所示的氨基酸序列的植物的开花时间; 并且该基因抑制开花刺激基因AGL20。 用于操作植物开花时间的方法包括过表达基因LOV1以延迟开花时间或抑制基因LOV1的表达以刺激开花时间。
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公开(公告)号：KR100647793B1
公开(公告)日：2006-11-23
申请号：KR1020060071233
申请日：2006-07-28
Applicant: 재단법인서울대학교산학협력재단
Abstract: A method for controlling flowering time of plant by using ECL1 gene is provided to induce the early or late flowering of plants, and increase production yield of flowers and seeds or production amount of leaf and stem. The flowering time of plant is regulated by regulating the expression of a polynucleotide encoding ECL1 protein having the amino acid sequence of SEQ ID NO:2, wherein the plant is Arabidopsis thaliana; the polynucleotide encoding the ECL1 protein has the nucleotide sequence of SEQ ID NO:1; the flowering time of Arabidopsis thaliana is promoted by overexpression of ECL1 gene in Arabidopsis thaliana by introducing a recombinant vector containing the polynucleotide encoding the ECL1 protein into Arabidopsis thaliana; the flowering time of Arabidopsis thaliana is delayed by deletion of ECL1 gene or combining the antisense molecule to the polynucleotide encoding the ECL1 protein in Arabidopsis thaliana; and the introduction of recombinant vector into Arabidopsis thaliana is accomplished by Agrobacterium sp.-mediated method, particle gun bombardment, silicon carbide whiskers, sonication, electroporation or PEG(polyethylene glycol) precipitation.
Abstract translation: 提供一种利用ECL1基因控制植物开花时间的方法，诱导植物早，晚花，提高花，种子产量或叶，茎产量。 通过调节编码具有SEQ ID NO：2的氨基酸序列的ECL1蛋白的多核苷酸的表达来调节植物的开花时间，其中所述植物是拟南芥; 编码ECL1蛋白质的多核苷酸具有SEQ ID NO：1的核苷酸序列; 通过将编码ECL1蛋白的多核苷酸的重组载体导入拟南芥中，通过在拟南芥中过表达ECL1基因来促进拟南芥的开花时间; 拟南芥的开花时间由于ECL1基因的缺失或反义分子与拟南芥中编码ECL1蛋白的多核苷酸的结合而延迟; 并且通过农杆菌介导的方法，粒子枪轰击，碳化硅晶须，超声处理，电穿孔或PEG（聚乙二醇）沉淀将重组载体引入拟南芥中。
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Patent Agency Ranking3.发明公开
公开(公告)号：KR1020040084186A
公开(公告)日：2004-10-06
申请号：KR1020030019069
申请日：2003-03-27
Applicant: 재단법인서울대학교산학협력재단
IPC: C07K14/415
CPC classification number: C07K14/415 , C12N15/8261 , C12N15/8271 , Y02A40/146
Abstract: PURPOSE: A gene controlling the root development of a plant and a method for manipulating the root development of a plant using the same gene are provided, thereby improving the phenotype of a plant related to root development, and facilitating the growth of it. CONSTITUTION: The gene GmEXP1 having the nucleotide sequence of SEQ ID NO:1 controls the root development of a plant, and encodes a protein controlling the root development of a plant, wherein the protein has the amino acid sequence of SEQ ID NO:2 and is isolated from Glycine max. A recombinant vector pGA643/GmEXP1 contains the gene GmEXP1. A transformed bacterium produced by transforming a bacterium with the recombinant vector pGA643/GmEXP1. The method for facilitating the root development of a plant comprises inserting the gene GmEXP1 into the expression vector, and introducing the expression vector into the plant.
Abstract translation: 目的：提供控制植物根系发育的基因和使用相同基因操作植物根系发育的方法，从而改善与根发育相关的植物的表型，并促进其生长。 构成：具有SEQ ID NO：1的核苷酸序列的基因GmEXP1控制植物的根部发育，并编码控制植物根系发育的蛋白质，其中该蛋白质具有SEQ ID NO：2的氨基酸序列和 与最大甘氨酸分离。 重组载体pGA643 / GmEXP1含有GmEXP1基因。 通过用重组载体pGA643 / GmEXP1转化细菌产生的转化细菌。 用于促进植物根系发育的方法包括将基因GmEXP1插入表达载体中，并将表达载体引入植物中。
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公开(公告)号：KR100648146B1
公开(公告)日：2006-11-29
申请号：KR1020060071234
申请日：2006-07-28
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N15/82
Abstract: A method for promoting the flowering time of plants by using AGL28 gene is provided to increase production yield of flowers and seeds by overexpressing the AGL28 gene in plants. The flowering time of plants is promoted by introducing a recombinant vector containing a polynucleotide encoding AGL28 protein having the amino acid sequence of SEQ ID NO:2 into Arabidopsis thaliana so as to overexpress the AGL28 gene, wherein the polynucleotide encoding AGL28 protein has the nucleotide sequence of SEQ ID NO:1; the AGL28 is isolated from Arabidopsis thaliana; and the recombinant vector is introduced into Arabidopsis thaliana by Agrobacterium sp.-mediated method, particle gun bombardment, silicon carbide whiskers, sonication, electroporation or polyethylene glycol(PEG) precipitation.
Abstract translation: 提供了通过使用AGL28基因来促进植物开花时间的方法，以通过在植物中过表达AGL28基因来增加花和种子的产量。 通过向拟南芥中导入含有编码具有SEQ ID NO：2的氨基酸序列的AGL28蛋白的多核苷酸的重组载体来促进植物的开花时间，从而过表达AGL28基因，其中编码AGL28蛋白的多核苷酸具有核苷酸序列 SEQ ID NO：1; AGL28从拟南芥中分离得到; 并通过农杆菌介导的方法，基因枪轰击，碳化硅晶须，超声处理，电穿孔或聚乙二醇（PEG）沉淀将重组载体导入拟南芥中。
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公开(公告)号：KR100637341B1
公开(公告)日：2006-10-23
申请号：KR1020030010772
申请日：2003-02-20
Applicant: 재단법인서울대학교산학협력재단
IPC: C07K14/415
CPC classification number: C12N15/827 , C07K14/415
Abstract: 본 발명은 식물의 개화시기 조절 유전자 및 이를 이용한 식물의 개화시기 조절방법에 관한 것이다. 보다 구체적으로, 본 발명은 애기장대(
Arabidopsis thaliana )로부터 분리된 식물의 개화시기 조절 유전자
LOV1 및 상기 유전자를 식물체에서 과다 발현시켜 식물체의 개화시기를 지연시키거나,
LOV1 유전자의 발현을 억제하여 식물체의 조기 개화를 유도하는 방법에 관한 것이다. 본 발명에서 분리된
LOV1 유전자 및 상기 유전자로부터 발현되는 LOV1 단백질은 식물의 개화 관련 형질의 개선 및 타 식물체에서 개화 시기 조절 유전자의 탐색 등에 유용하게 이용 될 수 있다. 또한, 본 발명에 따라 식물체의 개화시기를 촉진함으로써 꽃과 종자를 단시간 내에 생산하거나 또는 식물체의 개화시기를 지연시켜 영양생장을 지속적으로 유도함으로써 식물체로부터 잎이나 줄기와 같은 유용한 부분의 생산량을 증대시킬 수 있는 효과가 있다.
개화시기 조절, 유전자-
公开(公告)号：KR100833473B1
公开(公告)日：2008-05-29
申请号：KR1020070012375
申请日：2007-02-06
Applicant: 재단법인서울대학교산학협력재단
CPC classification number: C12N15/827 , C07K14/415
Abstract: An SVP(short vegetative phase) gene for controlling the flowering time is provided to mediate the signal transfer process in response to changes of ambient temperature and control the phase transition period in the development step of flower, so that the gene is useful for promoting the flowering time to produce rapidly flowers and seeds, or delaying the flowering time to increase production of leaf and stem. An SVP gene for controlling the flowering time has the nucleotide sequence of SEQ ID NO:1 and inhibits the expression of FT(flowering locus T) sequence by binding to CArG motif of the FT sequence. A transgenic plant is prepared by transforming a plant with a recombinant vector containing the SVP gene. The flowering time of a plant is delayed by overexpressing the SVP gene in the plant, or promoted by inhibiting expression of SVP gene in the plant, wherein the plant is Arabidopsis thaliana.
Abstract translation: 提供用于控制开花时间的SVP（短营养期）基因，以响应环境温度的变化介导信号转移过程，并控制花的发育步骤中的相变期，使得该基因可用于促进 开花时间快速生花和种子，或延迟开花时间，增加叶和茎的产量。 用于控制开花时间的SVP基因具有SEQ ID NO：1的核苷酸序列，并通过与FT序列的CArG基序结合来抑制FT（开花基因座T）序列的表达。 通过用含有SVP基因的重组载体转化植物来制备转基因植物。 通过在植物中过表达SVP基因延迟植物的开花时间，或通过抑制植物中SVP基因的表达来促进植物的开花时间，其中植物是拟南芥。
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公开(公告)号：KR100510055B1
公开(公告)日：2005-08-25
申请号：KR1020030019069
申请日：2003-03-27
Applicant: 재단법인서울대학교산학협력재단
IPC: C07K14/415
CPC classification number: C07K14/415 , C12N15/8261 , C12N15/8271 , Y02A40/146
Abstract: 본 발명은 식물의 뿌리 발달 조절 유전자 및 이를 이용한 식물의 뿌리 발달 촉진방법에 관한 것이다. 보다 구체적으로, 본 발명은 대두(
Glycine max )로부터 분리한 식물의 뿌리 발달 조절 유전자
GmEXP1, 상기 유전자에 의해 암호화되는 엑스판신(expansin) 단백질 및 상기 유전자를 식물체에서 과다 발현시킴으로써 식물의 뿌리 발달을 촉진하는 방법에 관한 것이다. 본 발명에서 분리된
GmEXP1 유전자 및 상기 유전자로부터 발현되는 엑스판신 단백질은 식물의 뿌리 발달과 관련된 형질의 개선 및 타 식물체에서 뿌리 발달 조절 유전자의 탐색 등에 유용하게 이용 될 수 있다. 또한, 본 발명에 따라 식물의 뿌리 발달을 촉진함으로써 식물체의 성장을 촉진시킬 수 있는 효과가 있다.
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