公开(公告)号：KR1020130065272A

公开(公告)日：2013-06-19

申请号：KR1020110132059

申请日：2011-12-09

Applicant: 한국전자통신연구원

Inventor： 백남섭 ,  김용희 ,  정상돈 ,  한영환 ,  정명애

IPC: A61B5/0478 ,  G01N27/327 ,  H01L27/04

Abstract: PURPOSE: A method for forming micro patterns of a neuronal signal measurement chip is provided to form the micro pattern with a desired shape and size without using a photo initiator, by forming the micro patterns of the neuronal signal measurement chip by using a photo cross linking polymer. CONSTITUTION: A substrate (10) is prepared, and surface treatment is performed with 70W plasma under Ar/N2 atmosphere for 10 minutes to form OH functional group. A bio-affinity polymer (12) of 50% is diluted to 2% density with ethanol, and the substrate where the OH functional group is formed is put into the diluted solution for 12 hours at room temperature for chemical bonding. The substrate which is chemically bonded to the bio-affinity polymer is cleaned with ethanol and is thermally-treated at 120°C for 10 minutes and then is cleaned with ultrasonic waves for 5 minutes to remove the bio-affinity polymer not bonded to the surface of the substrate. The cleaned substrate is cleaned with ethanol again and is dried with nitrogen.

Abstract translation: 目的：提供一种形成神经元信号测量芯片的微图案的方法，通过使用光交联形成神经信号测量芯片的微图案，从而形成具有所需形状和尺寸的微图案，而不使用光引发剂 聚合物。 构成：制备基板（10），在Ar / N 2气氛下用70W等离子体进行表面处理10分钟，形成OH官能团。 将50％的生物亲和聚合物（12）用乙醇稀释至2％密度，将形成有OH官能团的基质在室温下放入稀释溶液中12小时进行化学键合。 化学键合到生物亲和聚合物上的基质用乙醇清洗，并在120℃下热处理10分钟，然后用超声波清洗5分钟以除去未结合到表面的生物亲和聚合物 的基底。 再次用乙醇清洗清洁的基材并用氮气干燥。

公开(公告)号：KR101704729B1

公开(公告)日：2017-02-09

申请号：KR1020110130323

申请日：2011-12-07

Applicant: 한국전자통신연구원

Inventor： 김용희 ,  백남섭 ,  한영환 ,  정명애 ,  정상돈

IPC: G03F7/26 ,  G03F7/00

Abstract: 본발명은폴리디라이신(Poly-D-lysine)의결합을이용한미세패턴형성방법에관한것으로더욱상세하게는뉴런세포의특정영역에서의성장을유도하기위한폴리디라이신의결합을이용한미세패턴형성방법에관한것이다. 신경세포접착물질인폴리디라이신의공유결합을이용하여장시간세포성장을유도할수 있는미세패턴형성방법을제공하는데있다.

Abstract translation: 目的：提供使用聚-D-赖氨酸键的微图案化方法，以使用聚-D-赖氨酸的共价键长时间诱导细胞的生长。 构成：使用聚-D-赖氨酸键的微图案化方法包括：制备其上形成羟基官能团（11）的基材（10）的步骤; 用APTES（3-氨基丙基三乙氧基硅烷，12）化学键合基材表面的步骤; 在衬底上施加光致抗蚀剂并图案化光致抗蚀剂的步骤; 在图案化的光致抗蚀剂上形成戊二醛和聚-D-赖氨酸（19）的步骤; 以及去除图案化光致抗蚀剂的步骤。

公开(公告)号：KR1020130063778A

公开(公告)日：2013-06-17

申请号：KR1020110130326

申请日：2011-12-07

Applicant: 한국전자통신연구원

Inventor： 백남섭 ,  김용희 ,  한영환 ,  정상돈 ,  정명애

IPC: C07D333/10 ,  C07D409/04 ,  C07D409/14 ,  G01N33/52 ,  H01B1/12

CPC classification number: C07D333/10 ,  C07D409/04 ,  C07D409/14 ,  G01N33/52 ,  G01N2333/00 ,  H01B1/12

Abstract: PURPOSE: A conductive molecular wire is provided to improve signal-to-noise ratio value by surface treatment of an electrode for measuring a neural signal, and to ensure long-term stability by covalent bond with the electrode. CONSTITUTION: A conductive molecular wire has a structure of chemical formula 1. A method for preparing the molecular wire comprises: a step of adding zinc powder(3.8g, 58.0mmol) and dichlorodimethylsilane(7.0mL, 58.0mmol) to 120 ml of a dicholoromethane solution; a step of adding 4-iodobenzenesulfonyl choloride(5.0g, 16.5mmol) and n,n'-dimethylacetamide(4.6mL, 50.0mmol) and reacting at 70 deg. C for 2 hours; a step of adding acetyl chloride(1.53mL, 21.5mmol) and reacting at 50 deg. C for 15 minutes; a step of extracting with water and isolating by column chromatography to obtain a material of chemical formula 2.

Abstract translation: 目的：提供导电分子线，以通过用于测量神经信号的电极的表面处理来提高信噪比值，并通过与电极的共价键来确保长期稳定性。 导电分子线具有化学式1的结构。制备分子线的方法包括：将锌粉（3.8g，58.0mmol）和二氯二甲基硅烷（7.0mL，58.0mmol）加入到120ml的 二氯甲烷溶液; 加入4-碘苯磺酰氯（5.0g，16.5mmol）和n，N'-二甲基乙酰胺（4.6mL，50.0mmol）并在70℃反应的步骤。 C 2小时; 加入乙酰氯（1.53mL，21.5mmol）并在50℃下反应的步骤。 C 15分钟; 用水提取并通过柱色谱法分离以获得化学式2的材料的步骤。

公开(公告)号：KR1020130063776A

公开(公告)日：2013-06-17

申请号：KR1020110130323

申请日：2011-12-07

Applicant: 한국전자통신연구원

Inventor： 김용희 ,  백남섭 ,  한영환 ,  정명애 ,  정상돈

IPC: G03F7/26 ,  G03F7/00

Abstract: PURPOSE: A micro-patterning method using a poly-D-lysine bond is provided to induce the growth of cells for a long period of time using the covalent bond of poly-D-lysine. CONSTITUTION: A micro-patterning method using a poly-D-lysine bond includes: a step of preparing a substrate(10) on which a hydroxyl functional group(11) is formed; a step of chemically bonding the surface of the substrate with APTES(3-aminopropyltriethoxysilane, 12); a step of applying photoresist on the substrate and patterning the photoresist; a step of forming glutaraldehyde and poly-D-lysine(19) on the patterned photoresist; and a step of removing the patterned photoresist.

Abstract translation: 目的：提供使用聚-D-赖氨酸键的微图案化方法，以使用聚-D-赖氨酸的共价键长时间诱导细胞的生长。 构成：使用聚-D-赖氨酸键的微图案化方法包括：制备其上形成羟基官能团（11）的基材（10）的步骤; 用APTES（3-氨基丙基三乙氧基硅烷，12）化学键合基材表面的步骤; 在衬底上施加光致抗蚀剂并图案化光致抗蚀剂的步骤; 在图案化的光致抗蚀剂上形成戊二醛和聚-D-赖氨酸（19）的步骤; 以及去除图案化光致抗蚀剂的步骤。