公开(公告)号：US6280618B2

公开(公告)日：2001-08-28

申请号：US30292099

申请日：1999-04-30

Applicant: BIO RAD LABORATORIES

Inventor： WATKINS MICHAEL I ,  EDWARDS RICHARD B

IPC: C12Q1/70 ,  G01N15/14 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  G01N33/545 ,  G01N33/547 ,  G01N33/551 ,  G01N33/553 ,  B01D35/06

CPC classification number: G01N33/54326 ,  G01N33/537 ,  G01N33/54333 ,  G01N33/545 ,  G01N33/56994 ,  G01N33/582 ,  G01N33/6854 ,  Y10S435/973 ,  Y10T428/2982 ,  Y10T428/2984 ,  Y10T428/2987 ,  Y10T428/2989 ,  Y10T428/2991

Abstract: Heterogeneous assays for different analytes in a single biological sample are performed simultaneously in a multiplexed assay that combines flow cytometry with the use of magnetic particles as the solid phase and yields an individual result for each analyte. The particles are distinguishable from each other by characteristics that permit them to be differentiated into groups, each group carrying an assay reagent bonded to the particle surface that is distinct from the assay reagents of particles in other groups. The magnetic particles facilitate separation of the solid and liquid phases, permitting the assays to be performed by automated equipment. Assays are also disclosed for the simultaneous detection of antibodies of different classes and a common antigen specificity or of a common class and different antigen specificities. Each type is accomplished by immunological binding at the surfaces of two distinct solid phases in a sequential manner with dissociation of the binding and washing of the solid phase in between the binding steps.

Abstract translation: 在单一生物样品中的不同分析物的非均相测定在多重测定中同时进行，其结合流式细胞术和使用磁性颗粒作为固相，并为每种分析物产生单独的结果。 颗粒可通过允许将其分成组的特征彼此区分，每个组携带与颗粒表面结合的测定试剂，其不同于其它组中的颗粒的测定试剂。 磁性颗粒促进固相和液相的分离，允许通过自动化设备进行测定。 还公开了用于同时检测不同类型的抗体和共同抗原特异性或共同类别和不同抗原特异性的测定。 每种类型通过在两个不同的固相的表面之间的顺序方式通过在结合步骤之间的结合和洗涤固相的解离来实现。

公开(公告)号：AU1203199A

公开(公告)日：1999-06-07

申请号：AU1203199

申请日：1998-10-27

Applicant: BIO RAD LABORATORIES

Inventor： WATKINS MICHAEL I ,  EDWARDS RICHARD B

IPC: C12Q1/70 ,  G01N15/14 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  G01N33/545 ,  G01N33/547 ,  G01N33/551 ,  G01N33/553

Abstract: Heterogeneous assays for different analytes in a single biological sample are performed simultaneously in a multiplexed assay that combines flow cytometry with the use of magnetic particles as the solid phase and yields an individual result for each analyte. The particles vary in size to encompass distinct size ranges that are distinguishable by flow cytometry, the particles in each size range carrying an assay reagent bonded to the particle surface that is distinct from the assay reagents of particles in other size ranges. The magnetic particles facilitate separation of the solid and liquid phases, permitting the assays to be performed by automated equipment.

公开(公告)号：CA2260991C

公开(公告)日：2009-03-24

申请号：CA2260991

申请日：1998-10-27

Applicant: BIO RAD LABORATORIES

Inventor： EDWARDS RICHARD B ,  WATKINS MICHAEL I

IPC: G01N33/553 ,  C12Q1/70 ,  G01N15/10 ,  G01N15/14 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  G01N33/545 ,  G01N33/547 ,  G01N33/551 ,  G01N33/58

Abstract: Heterogeneous assays for different analytes in a single biological sample ar e performed simultaneously in a multiplexed assay that combines flow cytometry with the use of magnetic particles as the solid phase and yields an individual result for each analyte. The particles vary in size to encompass distinct size ranges that are distinguishable by flow cytometry, the particles in each size range carrying an assay reagent bonded to the particle surface that is distinct from the assay reagents of particles in other size ranges. The magnetic particles facilitate separation of the solid and liquid phases, permitting the assays to be performed by automated equipment.

公开(公告)号：CA2260991A1

公开(公告)日：1999-05-18

申请号：CA2260991

申请日：1998-10-27

Applicant: BIO RAD LABORATORIES

Inventor： EDWARDS RICHARD B ,  WATKINS MICHAEL I

IPC: C12Q1/70 ,  G01N15/14 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  G01N33/545 ,  G01N33/547 ,  G01N33/551 ,  G01N33/553 ,  G01N15/10

Abstract: Heterogeneous assays for different analytes in a single biological sample are performed simultaneously in a multiplexed assay that combines flow cytometry wit h the use of magnetic particles as the solid phase and yields an individual result for each analyte. The particles vary in size to encompass distinct size ranges that are distinguis hable by flow cytometry, the particles in each size range carrying an assay reagent bonded to the particle surface that is distinct from the assay reagents of particles in other size rang es. The magnetic particles facilitate separation of the solid and liquid phases, permitt ing the assays to be performed by automated equipment.

公开(公告)号：AU4169700A

公开(公告)日：2000-09-21

申请号：AU4169700

申请日：2000-03-02

Applicant: BIO RAD LABORATORIES

Inventor： WATKINS MICHAEL ,  EDWARDS RICHARD B

IPC: G01N33/543 ,  B32B27/18 ,  C12Q1/68 ,  G01J1/02 ,  G01J3/30 ,  G01N33/00

公开(公告)号：CA2406321C

公开(公告)日：2008-02-26

申请号：CA2406321

申请日：2001-04-06

Applicant: BIO RAD LABORATORIES

Inventor： KNIGHT TIMOTHY DARREN ,  MIRANDA PATRICIA A ,  DEL ROSARIO RENATO B ,  CHANG SUKNAN S ,  WATKINS MICHAEL I ,  EDWARDS RICHARD B

IPC: G01N33/53 ,  G01N33/78 ,  G01N15/00 ,  G01N15/14 ,  G01N33/543 ,  G01N33/58

Abstract: Immunological assays for several biologi-cal markers for thyroid disorders in a biological sample are performed in a single test with a combination of sandwich--type, sequential competitive, and serological assays by the use of particles classified into groups that are distinguishable by flow cytometry, one group for the assay of each marker. Each group of particles is coated with a different immuno-logical binding member, and coating densities, co-coating materials, and special buffer solutions are used to adjust for differences in the sensitivities and dynamic ranges of each of the markers in the typical sample.

公开(公告)号：CA2096199A1

公开(公告)日：1993-12-27

申请号：CA2096199

申请日：1993-05-13

Applicant: BIO RAD LABORATORIES

Inventor： HONIG JORDAN S ,  MACKO CHRISTOPHER J ,  EDWARDS RICHARD B

IPC: G01N1/00 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  A61J1/00

Abstract: REAGENT RECEPTACLE AND SUPPORT RACK FOR AUTOMATED CLINICAL ANALYZERS A reagent vessel and support rack for use in an automated clinical analyzers are disclosed. Characteristic features of the vessel are a membrane covering which is puncturable and preferably self-sealing, and characteristic features of the support rack are a rigid wall and retaining members to hold the vessel in the rack with the membrane against the rigid wall of the rack, thereby providing structural support to the membrane, with holes in the wall to permit access to the member and hence to the contents of the receptacle.