公开(公告)号：CA2406321C

公开(公告)日：2008-02-26

申请号：CA2406321

申请日：2001-04-06

Applicant: BIO RAD LABORATORIES

Inventor： KNIGHT TIMOTHY DARREN ,  MIRANDA PATRICIA A ,  DEL ROSARIO RENATO B ,  CHANG SUKNAN S ,  WATKINS MICHAEL I ,  EDWARDS RICHARD B

IPC: G01N33/53 ,  G01N33/78 ,  G01N15/00 ,  G01N15/14 ,  G01N33/543 ,  G01N33/58

Abstract: Immunological assays for several biologi-cal markers for thyroid disorders in a biological sample are performed in a single test with a combination of sandwich--type, sequential competitive, and serological assays by the use of particles classified into groups that are distinguishable by flow cytometry, one group for the assay of each marker. Each group of particles is coated with a different immuno-logical binding member, and coating densities, co-coating materials, and special buffer solutions are used to adjust for differences in the sensitivities and dynamic ranges of each of the markers in the typical sample.

公开(公告)号：AT364179T

公开(公告)日：2007-06-15

申请号：AT01924793

申请日：2001-04-06

Applicant: BIO RAD LABORATORIES

Inventor： WATKINS MICHAEL I ,  CHANG SUKNAN S ,  DEL ROSARIO RENATO B ,  KNIGHT TIMOTHY DARREN ,  MIRANDA PATRICIA A

IPC: G01N33/53 ,  G01N33/78 ,  G01N15/00 ,  G01N15/14 ,  G01N33/543

Abstract: Immunological assays for several biological markers for thyroid disorders in a biological sample are performed in a single test with a combination of sandwich-type, sequential competitive, and serological assays by the use of particles classified into groups that are distinguishable by flow cytometry, one group for the assay of each marker. Each group of particles is coated with a different immunological binding member, and coating densities, co-coating materials, and special buffer solutions are used to adjust for differences in the sensitivities and dynamic ranges of each of the markers in the typical sample.

公开(公告)号：DE60128794D1

公开(公告)日：2007-07-19

申请号：DE60128794

申请日：2001-04-06

Applicant: BIO RAD LABORATORIES

Inventor： WATKINS MICHAEL I ,  CHANG SUKNAN S ,  DEL ROSARIO RENATO B ,  KNIGHT TIMOTHY DARREN ,  MIRANDA PATRICIA A

IPC: G01N33/53 ,  G01N33/78 ,  G01N15/00 ,  G01N15/14 ,  G01N33/543

Abstract: Immunological assays for several biological markers for thyroid disorders in a biological sample are performed in a single test with a combination of sandwich-type, sequential competitive, and serological assays by the use of particles classified into groups that are distinguishable by flow cytometry, one group for the assay of each marker. Each group of particles is coated with a different immunological binding member, and coating densities, co-coating materials, and special buffer solutions are used to adjust for differences in the sensitivities and dynamic ranges of each of the markers in the typical sample.

公开(公告)号：DE60128794T2

公开(公告)日：2008-01-31

申请号：DE60128794

申请日：2001-04-06

Applicant: BIO RAD LABORATORIES

Inventor： WATKINS MICHAEL I ,  CHANG SUKNAN S ,  DEL ROSARIO RENATO B ,  KNIGHT TIMOTHY DARREN ,  MIRANDA PATRICIA A

IPC: G01N33/53 ,  G01N33/78 ,  G01N15/00 ,  G01N15/14 ,  G01N33/543

Abstract: Immunological assays for several biological markers for thyroid disorders in a biological sample are performed in a single test with a combination of sandwich-type, sequential competitive, and serological assays by the use of particles classified into groups that are distinguishable by flow cytometry, one group for the assay of each marker. Each group of particles is coated with a different immunological binding member, and coating densities, co-coating materials, and special buffer solutions are used to adjust for differences in the sensitivities and dynamic ranges of each of the markers in the typical sample.

公开(公告)号：CA2440664A1

公开(公告)日：2002-09-19

申请号：CA2440664

申请日：2002-03-13

Applicant: BIO RAD LABORATORIES

Inventor： DEL ROSARIO RENATO B ,  KNIGHT TIMOTHY D

IPC: G01N33/53 ,  C07K14/405 ,  G01N33/533 ,  G01N33/543 ,  G01N33/553 ,  G01N33/58 ,  G01N33/78 ,  C07C235/72 ,  C07C235/32

Abstract: The invention provides fluorescent analogues of thyroid hormones T3 and T4. Also provided are assays for thyroid hormones utilizing the compounds of the invention. The assays of the invention are in both one-step and two-step formats and allow the determination of both T3 and T4 concentration in a sample.

公开(公告)号：CA2406321A1

公开(公告)日：2001-10-25

申请号：CA2406321

申请日：2001-04-06

Applicant: BIO RAD LABORATORIES

Inventor： CHANG SUKNAN S ,  MIRANDA PATRICIA A ,  WATKINS MICHAEL I ,  DEL ROSARIO RENATO B ,  KNIGHT TIMOTHY DARREN

IPC: G01N33/53 ,  G01N15/00 ,  G01N15/14 ,  G01N33/543 ,  G01N33/78

Abstract: Immunological assays for several biological markers for thyroid disorders in a biological sample are performed in a single test with a combination of sandwich-type, sequential competitive, and serological assays by the use of particles classified into groups that are distinguishable by flow cytometry, one group for the assay of each marker. Each group of particles is coated wi th a different immunological binding member, and coating densities, co-coating materials, and special buffer solutions are used to adjust for differences i n the sensitivities and dynamic ranges of each of the markers in the typical sample.

公开(公告)号：WO0179844A2

公开(公告)日：2001-10-25

申请号：PCT/US0111314

申请日：2001-04-06

Applicant: BIO RAD LABORATORIES

Inventor： WATKINS MICHAEL I ,  CHANG SUKNAN S ,  DEL ROSARIO RENATO B ,  KNIGHT TIMOTHY DARREN ,  MIRANDA PATRICIA A

IPC: G01N33/53 ,  G01N15/00 ,  G01N15/14 ,  G01N33/543 ,  G01N33/78

CPC classification number: G01N33/78 ,  G01N15/14 ,  Y10S435/973

Abstract: Immunological assays for several biological markers for thyroid disorders in a biological sample are performed in a single test with a combination of sandwich-type, sequential competitive, and serological assays by the use of particles classified into groups that are distinguishable by flow cytometry, one group for the assay of each marker. Each group of particles is coated with a different immunological binding member, and coating densities, co-coating materials, and special buffer solutions are used to adjust for differences in the sensitivities and dynamic ranges of each of the markers in the typical sample.

Abstract translation: 通过使用分类为通过流式细胞术可区分的组的颗粒，通过夹心型，连续竞争性和血清学测定的组合，在单次测试中进行生物样品中几种甲状腺疾病生物标志物的免疫测定，一组 用于每个标记的测定。 使用不同的免疫结合构件涂覆每组颗粒，并且使用涂层密度，共涂层材料和特殊缓冲溶液来调整典型样品中每种标记物的灵敏度和动态范围的差异。