公开(公告)号：CA2818390C

公开(公告)日：2017-08-22

申请号：CA2818390

申请日：2011-11-18

Applicant: BIO-RAD LABORATORIES INC

Inventor： ARCHER ROBERT M ,  HSIUNG FRANK ,  PATT PAUL

IPC: C12Q1/04 ,  C12N5/07 ,  G01N1/28

Abstract: Adherent cells bearing characteristics that are detectable only in the adherent state can be sorted on the basis of these characteristics independently of their adherent state, by applying a transformable label to the entire population of cells, both those bearing the characteristics of interest and those not, in their adherent state and identifying the locations of the cells of interest on the adherent surface. The cells of interest, or all cells other than those of interest, are then selectively treated to transform the labels and achieve differentiation between the cells of interest and the remaining cells. All cells are then released from the adherent state and sorted in the same manner as non-adherent cells but on the basis of whether the labels are transformed or not transformed.