公开(公告)号：CA2808499C

公开(公告)日：2017-06-13

申请号：CA2808499

申请日：2011-08-19

Applicant: BIO-RAD LABORATORIES INC

Inventor： HENG XIN ,  PATT PAUL

IPC: G01N15/10

Abstract: Flow cytometry system includes a flow element through which a cell is transported in a flowing fluid. Flow element includes a bore bounded by a wall. Light source is configured to illuminate the cell. Optical system receives light emanating from the cell and directs at least some of received light to a light sensor. The optical system includes a numerical-aperture-increasing lens at a wall of the flow element. At least some of the received light passes through the numerical-aperture-increasing lens. The flow cytometry system may also include a beam splitter that directs two wavelength bands of emanating light such that light in two wavelength band preferentially reach different sensing locations via different paths. The system may also include an optical element placed in one of the paths, shifting a focal location of the affected path to compensate for chromatic aberration of the numerical-aperture-increasing lens.

公开(公告)号：CA2818390C

公开(公告)日：2017-08-22

申请号：CA2818390

申请日：2011-11-18

Applicant: BIO-RAD LABORATORIES INC

Inventor： ARCHER ROBERT M ,  HSIUNG FRANK ,  PATT PAUL

IPC: C12Q1/04 ,  C12N5/07 ,  G01N1/28

Abstract: Adherent cells bearing characteristics that are detectable only in the adherent state can be sorted on the basis of these characteristics independently of their adherent state, by applying a transformable label to the entire population of cells, both those bearing the characteristics of interest and those not, in their adherent state and identifying the locations of the cells of interest on the adherent surface. The cells of interest, or all cells other than those of interest, are then selectively treated to transform the labels and achieve differentiation between the cells of interest and the remaining cells. All cells are then released from the adherent state and sorted in the same manner as non-adherent cells but on the basis of whether the labels are transformed or not transformed.

公开(公告)号：SG10201703538UA

公开(公告)日：2017-06-29

申请号：SG10201703538U

申请日：2013-10-17

Applicant: BIO-RAD LABORATORIES INC

Inventor： BARICH JOHN ,  LEMAIRE-ADKINS RENEE ,  NEAZ DAVID ,  NOTCOVICH ARIEL ,  PATT PAUL ,  SHORT RYAN ,  SWIHART STEVEN ,  THRUSH EVAN

公开(公告)号：CA2827726C

公开(公告)日：2017-06-13

申请号：CA2827726

申请日：2012-02-21

Applicant: BIO-RAD LABORATORIES INC

Inventor： HENG XIN ,  PATT PAUL

IPC: G01N15/10

Abstract: Systems and methods for performing cytometry using a linear light sensor. An illumination field, a line scanned by the linear light sensor, or both are swept across a cell to be imaged. Relative motion between the cell and the swept illumination may be created using a movable optical component or components, by adhering cells to a plate and transporting the plate or by other techniques.

公开(公告)号：CA2771324C

公开(公告)日：2016-06-21

申请号：CA2771324

申请日：2010-08-20

Applicant: BIO-RAD LABORATORIES INC

Inventor： PATT PAUL

IPC: G01N15/10

Abstract: A cross sectional imaging system performs high-resolution, high-speed partial imaging of cells. Such a system may provide much of the information available from full imaging cytometry, but can be performed much more quickly, in part because the data analysis is greatly reduced in comparison with full image cytometry. The system includes a light source and a lens that focuses light from the light source onto a small spot in a scanning location. A transport mechanism causes relative motion between a cell in the scanning location and the spot. A sensor generates a signal indicating the intensity of light emanating from the cell as a result of illumination by the light source. The system repeatedly takes readings of the light intensity signal and characterizes the light intensity along a substantially linear path across the cell.