-
公开(公告)号：WO2002081729A2
公开(公告)日：2002-10-17
申请号：PCT/US2002/010815
申请日：2002-04-05
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY , ENZELBERGER, Markus, M. , HANSEN, Carl, L. , LIU, Jian , QUAKE, Stephen, R. , MA, Chiem
Inventor： ENZELBERGER, Markus, M. , HANSEN, Carl, L. , LIU, Jian , QUAKE, Stephen, R. , MA, Chiem
IPC: C12Q
CPC classification number: C12Q1/68 , B01F5/102 , B01F5/108 , B01F13/0059 , B01F15/06 , B01L3/50273 , B01L3/502738 , B01L7/525 , B01L9/527 , B01L2300/0861 , B01L2300/088 , B01L2300/0883 , B01L2300/0887 , B01L2300/123 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0481 , B01L2400/0655 , C12Q1/6844 , C12Q3/00 , C12Q2565/629
Abstract: The present invention provides microfluidic devices and methods using the same in various types of thermal cycling reactions. Certaom devices include a rotary microfluidic channel and a plurality of temperature regions at different locations along the rotary microfluidic channel at which temperature is regulated. Solution can be repeatedly passed through the temperature regions such that the solution is exposed to different temperatures. Other microfluidic devices include an array of reaction chambers formed by intersecting vertical and horizontal flow channels, with the ability to regulate temperature at the reaction chambers. The microfluidic devices can be used to conduct a number of different analyses, including various primer extension reactions and nucleic acid amplification reactions.
Abstract translation: 本发明提供了在各种类型的热循环反应中使用其的微流体装置和方法。 Certaom装置包括旋转微流体通道和沿着旋转微流体通道的不同位置处的多个温度区域，温度在该不同位置被调节。 溶液可以重复通过温度区域，使溶液暴露于不同的温度。 其他微流体装置包括通过相交的垂直和水平流动通道形成的反应室阵列，其具有调节反应室温度的能力。 微流体装置可用于进行多种不同的分析，包括各种引物延伸反应和核酸扩增反应。
-
Patent Agency Ranking
公开(公告)号：WO2005030925A3
公开(公告)日：2005-04-07
申请号：PCT/US2004/019982
申请日：2004-06-21
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY , LIU, Jian , HANSEN, Carl, L. , QUAKE, Stephen, R.
Inventor： LIU, Jian , HANSEN, Carl, L. , QUAKE, Stephen, R.
Abstract: A microfluidic device comprises a matrix (6000) of rotary flow reactors (6002). The microfluidic matrix device offers a solution to the "world-to-chip" interface problem by accomplishing two important goals simultaneously: an economy of scale in reagent consumption is achieved, while simultaneously minimizing pipetting steps. N 2 independent assays can be performed with only 2N+1 pipetting steps, using a single aliquot of enzyme amortized over all reactors. The chip reduces labor relative to conventional fluid handling techniques by using an order of magnitude less pipetting steps, and reduces cost by consuming two to three orders of magnitude less reagents per reaction. A PCR format has immediate applications in medical diagnosis and gene testing. Beyond PCR, the microfluidic matrix chip provides a universal and flexible platform for biological and chemical assays requiring parsimonious use of precious reagents and highly automated processing.
-
公开(公告)号：EP1345551B1
公开(公告)日：2009-04-01
申请号：EP01989783.4
申请日：2001-11-28
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： UNGER, Marc, A. , CHOU, Hou-Pu , THORSEN, Todd, A. , SCHERER, Axel , QUAKE, Stephen, R. , LIU, Jian , ADAMS, Mark, L. , HANSEN, Carl, L.
IPC: A61F2/00
CPC classification number: B05D3/04 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/046 , B01L2400/0481 , B01L2400/06 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , B65B31/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , C12Q1/6832 , C12Q1/6874 , C30B29/54 , F04B43/043 , F15C1/06 , F15C3/00 , F15C5/00 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T137/0379 , Y10T137/0424 , Y10T137/206 , Y10T137/2082 , Y10T137/2164 , Y10T137/2169 , Y10T137/2174 , Y10T137/2202 , Y10T137/2224 , Y10T137/3084 , Y10T137/7837 , Y10T137/86027 , Y10T156/10 , Y10T428/24479 , Y10T428/24612 , C12Q2535/125
Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
-
公开(公告)号：EP1670898A2
公开(公告)日：2006-06-21
申请号：EP04809458.5
申请日：2004-06-21
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： LIU, Jian , HANSEN, Carl, L. , QUAKE, Stephen, R.
IPC: C12N1/00
CPC classification number: B81B3/00 , B01J19/0093 , B01J2219/00783 , B01J2219/00828 , B01J2219/00833 , B01J2219/00891 , B01J2219/0095 , B01L3/5025 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/10 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/088 , B01L2300/123 , B01L2300/1827 , B01L2400/0481 , B01L2400/0655 , C12Q1/68 , F04B43/043 , G01N2035/00158
Abstract: A microfluidic device comprises a matrix (6000) of rotary flow reactors (6002). The microfluidic matrix device offers a solution to the 'world-to-chip' interface problem by accomplishing two important goals simultaneously: an economy of scale in reagent consumption is achieved, while simultaneously minimizing pipetting steps. N2 independent assays can be performed with only 2N+1 pipetting steps, using a single aliquot of enzyme amortized over all reactors. The chip reduces labor relative to conventional fluid handling techniques by using an order of magnitude less pipetting steps, and reduces cost by consuming two to three orders of magnitude less reagents per reaction. A PCR format has immediate applications in medical diagnosis and gene testing. Beyond PCR, the microfluidic matrix chip provides a universal and flexible platform for biological and chemical assays requiring parsimonious use of precious reagents and highly automated processing.
-
5.发明公开MICROFABRICATED ELASTOMERIC VALVE AND PUMP SYSTEMS 有权MIKROHERGESTELLTE ELASTOMERE VENTIL- UND PUMPSYSTEME
公开(公告)号：EP1345551A2
公开(公告)日：2003-09-24
申请号：EP01989783.4
申请日：2001-11-28
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： UNGER, Marc, A. , CHOU, Hou-Pu , THORSEN, Todd, A. , SCHERER, Axel , QUAKE, Stephen, R. , LIU, Jian , ADAMS, Mark, L. , HANSEN, Carl, L.
IPC: A61F2/00
CPC classification number: B05D3/04 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/046 , B01L2400/0481 , B01L2400/06 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , B65B31/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , C12Q1/6832 , C12Q1/6874 , C30B29/54 , F04B43/043 , F15C1/06 , F15C3/00 , F15C5/00 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T137/0379 , Y10T137/0424 , Y10T137/206 , Y10T137/2082 , Y10T137/2164 , Y10T137/2169 , Y10T137/2174 , Y10T137/2202 , Y10T137/2224 , Y10T137/3084 , Y10T137/7837 , Y10T137/86027 , Y10T156/10 , Y10T428/24479 , Y10T428/24612 , C12Q2535/125
Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
Abstract translation: 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有形成沿所述第二弹性体层的底表面延伸的第二凹槽的第二凸起突起; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道形成在第一和第二弹性体层之间的第二凹部中; 以及将所述第一弹性体层定位在平面基底的顶部上，使得在所述第一弹性体层和所述平面基底之间的所述第一凹部中形成流动通道。
-
公开(公告)号：EP1384022A2
公开(公告)日：2004-01-28
申请号：EP02763966.5
申请日：2002-04-05
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY , Enzelberger, Markus M. , Hansen, Carl L. , Liu, Jian , Quake, Stephen R. , Ma, Chiem
Inventor： ENZELBERGER, Markus, M. , HANSEN, Carl, L. , LIU, Jian , QUAKE, Stephen, R. , MA, Chiem
IPC: F16K17/00 , F04B19/24 , F04B17/00 , F28D21/00 , C12M1/00 , C12P19/34 , C12Q1/68 , C23C16/00 , F27B5/14 , G01N21/01 , G01N27/26 , G01N27/447 , C07K1/04 , C07K17/06 , C07K17/14 , B01J19/00
CPC classification number: C12Q1/68 , B01F5/102 , B01F5/108 , B01F13/0059 , B01F15/06 , B01L3/50273 , B01L3/502738 , B01L7/525 , B01L9/527 , B01L2300/0861 , B01L2300/088 , B01L2300/0883 , B01L2300/0887 , B01L2300/123 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0481 , B01L2400/0655 , C12Q1/6844 , C12Q3/00 , C12Q2565/629
Abstract: The present invention provides microfluidic devices and methods using the same in various types of thermal cycling reactions. Certaom devices include a rotary microfluidic channel and a plurality of temperature regions at different locations along the rotary microfluidic channel at which temperature is regulated. Solution can be repeatedly passed through the temperature regions such that the solution is exposed to different temperatures. Other microfluidic devices include an array of reaction chambers formed by intersecting vertical and horizontal flow channels, with the ability to regulate temperature at the reaction chambers. The microfluidic devices can be used to conduct a number of different analyses, including various primer extension reactions and nucleic acid amplification reactions.
Abstract translation: 本发明提供了在各种类型的热循环反应中使用其的微流体装置和方法。 Certaom装置包括旋转微流体通道和沿着旋转微流体通道的不同位置处的多个温度区域，温度在该不同位置被调节。 溶液可以重复通过温度区域，使溶液暴露于不同的温度。 其他微流体装置包括通过相交的垂直和水平流动通道形成的反应室阵列，其具有调节反应室温度的能力。 微流体装置可用于进行许多不同的分析，包括各种引物延伸反应和核酸扩增反应。
-
-
-
-
-
Search Results
6
records
(took 0.031 seconds)
