公开(公告)号：JP2012228268A

公开(公告)日：2012-11-22

申请号：JP2012167346

申请日：2012-07-27

Applicant: Fluidigm Corp ,  フルイディグム コーポレイション

Inventor： CHOU HOU-PU ,  DARIDON ANTOINE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY ,  JAVADI SHERVIN ,  LIAU YISH-HANN ,  MANGER IAN D ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  THRONDSET WILLIAM

IPC: C12M1/34 ,  G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M3/00 ,  C12N1/00 ,  C12N5/071 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

CPC classification number: C12M1/34 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0655 ,  C12M21/06 ,  C12M23/16 ,  G01N15/1484 ,  G01N33/4833 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2015/1493 ,  G02B21/32

Abstract: PROBLEM TO BE SOLVED: To provide novel methods for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles.SOLUTION: The invention includes apparatus, methods, and kits for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention includes apparatus, methods, and kits for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order, and employed for any suitable number of times within a system.

Abstract translation: 要解决的问题：提供微流控操作和/或分析颗粒如细胞，病毒，细胞器，珠粒和/或囊泡的新方法。 解决方案：本发明包括用于微流控操作和/或检测颗粒例如细胞和/或珠的装置，方法和试剂盒。 本发明包括用于微粒操作和/或分析颗粒如细胞，病毒，细胞器，珠粒和/或囊泡的装置，方法和试剂盒。 本发明还提供用于进行这些操作和分析的微流体机理。 这些机制可以实现颗粒的控制输入，运动/定位，保留/定位，处理，测量，释放和/或输出。 此外，这些机构可以以任何合适的顺序组合，并且在系统内使用任何合适的次数。 版权所有（C）2013，JPO＆INPIT

公开(公告)号：JP2010042020A

公开(公告)日：2010-02-25

申请号：JP2009236738

申请日：2009-10-13

Applicant: Fluidigm Corp ,  フルイディグム コーポレイション

Inventor： CHOU HOU-PU ,  DARIDON ANTOINE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY ,  JAVADI SHERVIN ,  LIAU YISH-HANN ,  MANGER IAN D ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  THRONDSET WILLIAM

IPC: C12Q1/02 ,  G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  G01N15/02 ,  G01N15/14

CPC classification number: C12M1/34 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0655 ,  C12M21/06 ,  C12M23/16 ,  G01N15/1484 ,  G01N33/4833 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2015/1493 ,  G02B21/32

Abstract: PROBLEM TO BE SOLVED: To provide a new method for microfluidic manipulation and/or analysis of particles (e.g., cells, viruses, organelles, beads, and/or vesicles). SOLUTION: A microfluidic particle-analysis system includes a device, a method, and a kit for the microfluidic manipulation and/or detection of the particles such as cells and/or beads. The system includes the device, the method and the kit for the microfluidic manipulation and/or analysis of the particles such as the cells, viruses, organelles, beads and/or vesicles, and microfluidic mechanisms are provided for performing such the manipulations and analyses. Such mechanisms enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of the particles. Further, these mechanisms can be combined in any suitable order and employed in any suitable number of times within the system. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供用于微粒操作和/或分析颗粒（例如细胞，病毒，细胞器，珠粒和/或囊泡）的新方法。 解决方案：微流体颗粒分析系统包括用于微流体操纵和/或检测诸如细胞和/或珠粒的微流体的装置，方法和试剂盒。 该系统包括用于微粒操作和/或分析细胞，病毒，细胞器，珠粒和/或囊泡的微流体操作和/或分析的装置，方法和试剂盒，以及用于进行这种操作和分析的微流体机制。 这样的机构使得能够控制输入，移动/定位，保留/定位，处理，测量，释放和/或输出颗粒。 此外，这些机构可以以任何合适的顺序组合并且在系统内以任何合适的次数使用。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：WO2007044091A3

公开(公告)日：2007-11-15

申请号：PCT/US2006021416

申请日：2006-06-02

Applicant: FLUIDIGM CORP ,  HEID CHRISTIAN A ,  DARIDON ANTOINE

Inventor： HEID CHRISTIAN A ,  DARIDON ANTOINE

IPC: G01N33/53

CPC classification number: B01L3/5027 ,  B01L7/52 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/301

Abstract: The invention relates to methods, reagents and devices for detection and characterization of nucleic acids, cells, and other biological samples. Assay method are provided in which a sample is partitioned into sub-samples, and analysis of the contents of the sub-samples carried out. The invention also provides microfluidic devices for conducting the assay. The invention also provides an analysis method using a universal primers and probes for amplification and detection.

Abstract translation: 本发明涉及用于检测和表征核酸，细胞和其他生物样品的方法，试剂和装置。 提供了一种分析方法，其中将样品分成子样品，并分析所进行的子样品的含量。 本发明还提供用于进行测定的微流体装置。 本发明还提供了一种使用通用引物和探针进行扩增和检测的分析方法。

公开(公告)号：WO2006071470A2

公开(公告)日：2006-07-06

申请号：PCT/US2005044072

申请日：2005-12-05

Applicant: CALIFORNIA INST OF TECHN ,  FLUIDIGM CORP ,  UNIV CALIFORNIA ,  SIEMENS CORP ,  LEE CHUNG-CHENG ,  SUI GUODONG ,  ELIZAROV ARKADIJ ,  KOLB HARTMUTH C ,  HUANG JIANG ,  HEATH JAMES R ,  PHELPS MICHAEL E ,  QUAKE STEPHEN R ,  TSENG HSIAN-RONG ,  WYATT PAUL ,  DARIDON ANTOINE

Inventor： LEE CHUNG-CHENG ,  SUI GUODONG ,  ELIZAROV ARKADIJ ,  KOLB HARTMUTH C ,  HUANG JIANG ,  HEATH JAMES R ,  PHELPS MICHAEL E ,  QUAKE STEPHEN R ,  TSENG HSIAN-RONG ,  WYATT PAUL ,  DARIDON ANTOINE

CPC classification number: B01J19/0093 ,  B01F11/0042 ,  B01F13/0059 ,  B01F2215/0404 ,  B01J2219/00286 ,  B01J2219/00396 ,  B01J2219/00398 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/00599 ,  B01J2219/00731 ,  B01J2219/00759 ,  B01J2219/00783 ,  B01J2219/00822 ,  B01J2219/00824 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00907 ,  B01L3/5027 ,  B33Y80/00 ,  C07B59/00

Abstract: New microfluidic devices, useful for carrying out chemical reactions, are provided. The devices are adapted for on-chip solvent exchange, chemical processes requiring multiple chemical reactions, and rapid concentration of reagents.

Abstract translation: 提供了用于进行化学反应的新型微流体装置。 该器件适用于片上溶剂交换，需要多次化学反应的化学过程和试剂的快速浓缩。

公开(公告)号：AU2003224817B2

公开(公告)日：2008-11-06

申请号：AU2003224817

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： DARIDON ANTOINE ,  MANGER IAN D ,  LIAU YISH-HANN ,  NASSEF HANY RAMEZ ,  THRONDSET WILLIAM ,  CHOU HOU-PU ,  HAO CUNSHENG CASEY ,  NORTON PIERCE ,  FOWLER BRIAN ,  FARRELL KEVIN ,  JAVADI SHERVIN

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

公开(公告)号：AU2003224817A1

公开(公告)日：2003-10-20

申请号：AU2003224817

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： JAVADI SHERVIN ,  LIAU YISH-HANN ,  MANGER IAN D ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  THRONDSET WILLIAM ,  CHOU HOU-PU ,  DARIDON ANTOINE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

公开(公告)号：CA2480728A1

公开(公告)日：2003-10-16

申请号：CA2480728

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： MANGER IAN D ,  THRONDSET WILLIAM ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY ,  LIAU YISH-HANN ,  JAVADI SHERVIN ,  DARIDON ANTOINE ,  CHOU HOU-PU

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

Abstract: The invention provides systems (2000), including microfluidic mechanisms, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. These mechanisms may enable controlle d input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any number of suitable times in the system to allow particles to be sorted, cultured, mixed, treate d, and/or assayed, among others. These combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.

公开(公告)号：EP1915618A4

公开(公告)日：2009-09-30

申请号：EP06836075

申请日：2006-06-02

Applicant: FLUIDIGM CORP

Inventor： HEID CHRISTIAN A ,  DARIDON ANTOINE

IPC: G01N33/53

CPC classification number: B01L3/5027 ,  B01L7/52 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/301

公开(公告)号：EP1838431A4

公开(公告)日：2012-08-22

申请号：EP05857049

申请日：2005-12-05

Applicant: CALIFORNIA INST OF TECHN ,  FLUIDIGM CORP ,  UNIV CALIFORNIA ,  SIEMENS CORP

Inventor： LEE CHUNG-CHENG ,  SUI GUODONG ,  ELIZAROV ARKADIJ ,  KOLB HARTMUTH C ,  HUANG JIANG ,  HEATH JAMES R ,  PHELPS MICHAEL E ,  QUAKE STEPHEN R ,  TSENG HSIAN-RONG ,  WYATT PAUL ,  DARIDON ANTOINE

IPC: B32B37/00 ,  B01F11/00 ,  B01F13/00 ,  B01J19/00 ,  B44C7/00 ,  C07B59/00

CPC classification number: B01J19/0093 ,  B01F11/0042 ,  B01F13/0059 ,  B01F2215/0404 ,  B01J2219/00286 ,  B01J2219/00396 ,  B01J2219/00398 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/00599 ,  B01J2219/00731 ,  B01J2219/00759 ,  B01J2219/00783 ,  B01J2219/00822 ,  B01J2219/00824 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/0086 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00907 ,  B01L3/5027 ,  B33Y80/00 ,  C07B59/00

公开(公告)号：EP1499706A4

公开(公告)日：2010-11-03

申请号：EP03721509

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： CHOU HOU-PU ,  DARIDON ANTOINE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY ,  JAVADI SHERVIN ,  LIAU YISH-HANN ,  MANGER IAN D ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  THRONDSET WILLIAM

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

CPC classification number: C12M1/34 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0655 ,  C12M21/06 ,  C12M23/16 ,  G01N15/1484 ,  G01N33/4833 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2015/1493 ,  G02B21/32

Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.