公开(公告)号：WO2007033385A3

公开(公告)日：2007-07-12

申请号：PCT/US2006036365

申请日：2006-09-13

Applicant: FLUIDIGM CORP ,  NASSEF HANY RAMEZ ,  CHOU HOU-PU ,  LUCERO MICHAEL ,  MAY ANDREW ,  YOKOBATA KATHY

Inventor： NASSEF HANY RAMEZ ,  CHOU HOU-PU ,  LUCERO MICHAEL ,  MAY ANDREW ,  YOKOBATA KATHY

IPC: G01N33/543 ,  B01L3/00

CPC classification number: G01N33/54366 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00389 ,  B01J2219/00398 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0655 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N33/5302

Abstract: A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.

Abstract translation: 微流体装置适于执行许多同时结合测定，包括但不限于免疫学实验，例如ELISA测定，初级和次级抗体之间的最小串扰。

公开(公告)号：WO2004089810A2

公开(公告)日：2004-10-21

申请号：PCT/US2004010492

申请日：2004-04-05

Applicant: FLUIDIGM CORP ,  MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

IPC: B01L3/00 ,  B01L3/02 ,  B01L7/00 ,  B01L99/00 ,  B81B20060101 ,  C12Q1/68 ,  G01N1/10 ,  B81B

CPC classification number: B01L3/00 ,  B01L3/5027 ,  B01L3/502723 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0638 ,  C12Q1/686

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。

公开(公告)号：CA2807591A1

公开(公告)日：2004-10-21

申请号：CA2807591

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

IPC: C12M1/40 ,  B81B3/00 ,  B81B7/04 ,  C12M1/34 ,  C12M1/38 ,  C12P19/34 ,  C12Q1/68 ,  G01N1/00

公开(公告)号：CA2467587A1

公开(公告)日：2003-06-12

申请号：CA2467587

申请日：2002-11-27

Applicant: FLUIDIGM CORP

Inventor： WIENECKE ANJA ,  YI YONG ,  FACER GEOFFREY ,  UNGER MARC A ,  LUCERO MICHAEL ,  MIYASHITA-LIN EMILY ,  MANGER IAN DAVID

IPC: G01N33/53 ,  B01F13/00 ,  B01L3/00 ,  C12M1/00 ,  C12Q1/25 ,  C12Q1/68 ,  C40B40/02 ,  C40B50/06 ,  C40B60/12 ,  C40B60/14 ,  F04B43/02 ,  G01N1/00 ,  G01N1/28 ,  G01N27/447 ,  G01N35/00 ,  G01N37/00 ,  C12M1/36 ,  G01N15/06 ,  C12N11/16 ,  C12P19/34

Abstract: A variety of elastomeric-based microfluidic devices and methods for using an d manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagent s have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and ge ne expression analyses.

公开(公告)号：EP1463796A4

公开(公告)日：2006-03-08

申请号：EP02786834

申请日：2002-11-27

Applicant: FLUIDIGM CORP

Inventor： UNGER MARC A ,  MANGER IAN DAVID ,  LUCERO MICHAEL ,  YI YONG ,  MIYASHITA-LIN EMILY ,  WIENECKE ANJA ,  FACER GEOFFREY

IPC: G01N33/53 ,  B01F13/00 ,  B01L3/00 ,  C12M1/00 ,  C12Q1/25 ,  C12Q1/68 ,  C40B40/02 ,  C40B50/06 ,  C40B60/12 ,  C40B60/14 ,  F04B43/02 ,  G01N1/00 ,  G01N1/28 ,  G01N27/447 ,  G01N35/00 ,  G01N37/00 ,  C12M1/36 ,  C12N11/16 ,  C12P19/34 ,  G01N15/06

CPC classification number: C12M1/38 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/68 ,  C12Q1/6844 ,  C40B60/14 ,  F16K11/20 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T137/2076 ,  Y10T137/2496 ,  Y10T137/86292 ,  Y10T137/87249 ,  Y10T436/2575 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经被预先沉积。 一旦将样品引入反应部位，试剂就会停止。 这些装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。

公开(公告)号：CA2807591C

公开(公告)日：2014-11-18

申请号：CA2807591

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

IPC: C12M1/40 ,  B81B3/00 ,  B81B7/04 ,  C12M1/34 ,  C12M1/38 ,  C12P19/34 ,  C12Q1/68 ,  G01N1/00

Abstract: Introducing a sample solution that includes a target nucleic acid polymer into a microfluidic device that has a sample region with: (i) a flow channel in fluid communication with one fluid inlet and one fluid outlet; and (ii) a plurality of valves that, when closed, partition the flow channel into isolated reaction sites. Each valve is formed from an elastomeric membrane that separates a control channel from the flow channel and which is disposed to be deflected into or retracted out from the flow channel in response to an actuation force to the control channel, thereby forming the valve. The method includes closing the valves, thereby partitioning the flow channel into isolated reaction sites containing portions of said sample solution; exposing the reaction sites to conditions under which the nucleic acid polymer is amplified to produce an amplification product; and opening said valves and displacing sample solution out of the flow channel.

公开(公告)号：AU2002351187A1

公开(公告)日：2003-06-17

申请号：AU2002351187

申请日：2002-11-27

Applicant: FLUIDIGM CORP

Inventor： UNGER MARC A ,  MANGER IAN DAVID ,  LUCERO MICHAEL ,  YI YONG ,  MIYASHITA-LIN EMILY ,  WIENECKE ANJA ,  FACER GEOFFREY

IPC: G01N33/53 ,  B01F13/00 ,  B01L3/00 ,  C12M1/00 ,  C12Q1/25 ,  C12Q1/68 ,  C40B40/02 ,  C40B50/06 ,  C40B60/12 ,  C40B60/14 ,  F04B43/02 ,  G01N1/00 ,  G01N1/28 ,  G01N27/447 ,  G01N35/00 ,  G01N37/00 ,  C12M1/36 ,  C12N11/16 ,  G01N15/06 ,  C12P19/34

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

公开(公告)号：EP1615721A4

公开(公告)日：2006-08-02

申请号：EP04758896

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

IPC: B01L3/02 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B81B20060101 ,  C12Q1/68 ,  G01N1/10

CPC classification number: B01L3/00 ,  B01L3/5027 ,  B01L3/502723 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0638 ,  C12Q1/686

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

公开(公告)号：CA2807564C

公开(公告)日：2014-01-28

申请号：CA2807564

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

IPC: B81B3/00 ,  B81B7/04 ,  C12M1/34 ,  C12M1/40 ,  C12Q1/68 ,  C40B30/00 ,  C40B60/00 ,  G01N33/48

Abstract: A microfluidic device, with elastomeric components, and having a flow channel, wherein said flow channel has a plurality of blind flow channels opening into and in fluid communication with the flow channel, each blind flow channel having an aperture portion, a channel portion and a terminus portion, and wherein a region at the terminus of each blind flow channel defines a reaction site. Each blind flow channel is associated with a valve that when closed isolates the reaction site from the flow channel. The valves associated with each of the plurality of blind flow channels in fluid communication with the flow channel are under the control of a common control channel and are coordinately closed or opened.

公开(公告)号：CA2521171C

公开(公告)日：2013-05-28

申请号：CA2521171

申请日：2004-04-05

Applicant: FLUIDIGM CORP

Inventor： MCBRIDE LINCOLN ,  LUCERO MICHAEL ,  UNGER MARC ,  NASSEF HANY RAMEZ ,  FACER GEOFFREY

IPC: B01L3/02 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B81B20060101 ,  B81B3/00 ,  C12M1/34 ,  C12M1/40 ,  C12Q1/68 ,  G01N1/10 ,  G01N33/48

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.