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    Dispositivo de observación de células, dispositivo de estimulación eléctrica y método de observación de células
    1.
    发明专利
    Dispositivo de observación de células, dispositivo de estimulación eléctrica y método de observación de células 未知

    公开(公告)号：ES2915663T3

    公开(公告)日：2022-06-24

    申请号：ES18209767

    申请日：2014-11-12

    Applicant: HAMAMATSU PHOTONICS KK

    Inventor： SAITO NATSUMI ,  ITO TAIRA ,  KATAOKA TAKUJI

    IPC: C12M1/42 ,  C12Q1/02 ,  G01N21/64

    Abstract: Un dispositivo de estimulación eléctrica (16) para insertar en una microplaca (20), que tiene múltiples pocillos (21) que contienen una muestra (S) que incluye una célula, y aplicar estimulación eléctrica a la célula, comprendiendo el dispositivo de estimulación eléctrica: múltiples pares de electrodos (17), incluyendo cada uno un primer electrodo (17a) y un segundo electrodo (17b), extendiéndose tanto el primer electrodo como el segundo electrodo dentro del pocillo de la microplaca en la dirección de profundidad del pocillo, estando los múltiples pares de electrodos (17) dispuestos en una forma bidimensional correspondiente a la matriz bidimensional de los múltiples pocillos (21) de la microplaca (20) para extenderse hacia los pocillos (21) de la microplaca (20); y una unidad de ajuste de posición (71) adaptada para ajustar simultáneamente las posiciones relativas de una punta del primer electrodo (17a) a una punta del segundo electrodo (17b) en los múltiples pares de electrodos.

    2.
    发明专利
    未知

    公开(公告)号：DE69219963D1

    公开(公告)日：1997-07-03

    申请号：DE69219963

    申请日：1992-02-13

    Applicant: NIHON MILLIPORE KOGYO K K ,  HAMAMATSU PHOTONICS KK

    Inventor： HIROSE ATSUMI ,  SETO SUSUMU ,  KATAOKA TAKUJI

    IPC: C12Q1/06 ,  C12Q1/24 ,  C12Q1/66 ,  C12Q1/68

    Abstract: A method of determining a viable count rapidly and conveniently at high sensitivity. The method comprises preparing a filtration membrane element wherein hydrophilic sections are substantially completely isolated from each other with partitions, using a particulate spray to add an extracting reagent and a luminescent reagent in such amounts as to wet the filtration membrane in each section, and treating the obtained specimen with a high-sensitivity bioluminescent image analysis system. The use of the spray allows the reagents and extract to be completely retained in each hydrophilic section, and a combination thereof with the bioluminescent image analysis allows the viable count to be determined rapidly without completely or substantially necessitating culturing.

    4.
    发明专利
    未知

    公开(公告)号：DE69219963T2

    公开(公告)日：1997-10-16

    申请号：DE69219963

    申请日：1992-02-13

    Applicant: NIHON MILLIPORE KOGYO K K ,  HAMAMATSU PHOTONICS KK

    Inventor： HIROSE ATSUMI ,  SETO SUSUMU ,  KATAOKA TAKUJI

    IPC: C12Q1/06 ,  C12Q1/24 ,  C12Q1/66 ,  C12Q1/68

    Abstract: A method of determining a viable count rapidly and conveniently at high sensitivity. The method comprises preparing a filtration membrane element wherein hydrophilic sections are substantially completely isolated from each other with partitions, using a particulate spray to add an extracting reagent and a luminescent reagent in such amounts as to wet the filtration membrane in each section, and treating the obtained specimen with a high-sensitivity bioluminescent image analysis system. The use of the spray allows the reagents and extract to be completely retained in each hydrophilic section, and a combination thereof with the bioluminescent image analysis allows the viable count to be determined rapidly without completely or substantially necessitating culturing.

    CELL OBSERVATION DEVICE, ELECTRICAL STIMULATION DEVICE, AND CELL OBSERVATION METHOD
    8.
    发明公开
    CELL OBSERVATION DEVICE, ELECTRICAL STIMULATION DEVICE, AND CELL OBSERVATION METHOD 审中-公开
    ZELLBEOBACHTUNGSVORRICHTUNG，ELEKTRISCHE STIMULATIONSVORRICHTUNG UND ZELLBEOBACHTUNGSVERFAHREN

    公开(公告)号：EP2913390A4

    公开(公告)日：2016-06-29

    申请号：EP13849414

    申请日：2013-10-23

    Applicant: HAMAMATSU PHOTONICS KK

    Inventor： KATAOKA TAKUJI ,  ITO TAIRA ,  SAITO NATSUMI

    IPC: C12M1/34 ,  A61N1/32 ,  C12Q1/02 ,  G01N15/10 ,  G01N15/14 ,  G01N33/487

    CPC classification number: G01N21/6428 ,  G01N33/4836 ,  G01N33/48728 ,  G01N33/5005 ,  G01N2201/022

    Abstract: A cell observation system 1 is a cell observation system 1 for observing a cell held by a microplate 20 having a plurality of wells 21 arranged therein for holding a sample S including the cell and comprises a microplate holder 11 for mounting the microplate 20, an electrical stimulator 16 arranged with a plurality of electrode pairs 17 including positive and negative electrodes 17b, 17a, and a data analyzer 50 for controlling a position of the electrical stimulator 16 so as to place the electrode pairs 17 within the wells 21 of the microplate 20, while a leading end of the negative electrode 17a on the well 21 side extends longer than a leading end of the positive electrode 17b on the well 21 side.

    Abstract translation: 细胞观察系统1是细胞观察系统1，用于观察由微孔板20保持的细胞，其具有布置在其中的多个孔21，用于保持包括细胞的样品S，并且包括用于安装微孔板20的微板保持器11， 激励器16布置有包括正极和负极17b，17a的多个电极对17，以及用于控制电刺激器16的位置以将电极对17放置在微板20的孔21内的数据分析器50， 而在井21侧的负极17a的前端比阱21侧的正极17b的前端延伸。

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