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公开(公告)号：US11202839B2
公开(公告)日：2021-12-21
申请号：US15807844
申请日：2017-11-09
Applicant: INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY , INSTITUTE FOR BASIC SCIENCE
Inventor： Dong Wook Kim , Jin Soo Kim , Chul Yong Park , Duk Hyoung Kim , Jung Eun Kim , Jiyeon Kweon
IPC: A61K48/00 , C12N5/074 , C12N9/22 , C12N15/11 , A61K35/545 , C07K14/755 , C12N15/113 , A61K35/12
Abstract: The present invention provides a method for inducing an inversion of normal blood coagulation factor VIII (F8) gene, a method for correcting an inversion of blood coagulation factor VIII gene in which the inversion has occurred, and a Hemophilia A patient-derived induced pluripotent stem cell in which the inversion is corrected, constructed using the same. The method of the present invention effectively reproduces the inversion of intron 1 and intron 22 of the F8 gene, which is responsible for the majority of severe hemophilia A, and thereby may be effectively used for studying the development mechanism of hemophilia A and as a research tool for screening therapeutic agents. The inversion-corrected induced pluripotent stem cell constructed according the method of the present invention enables an efficient and fundamental treatment for hemophilia A by restoring a genotype in which mutation has occurred to a wild type-like state, without limitation via normal gene or protein delivery.
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公开(公告)号：US11136588B2
公开(公告)日：2021-10-05
申请号：US15766432
申请日：2016-10-06
Inventor： Jin Soo Kim , Jungeun Kim , Sunghwa Choe , Je Wook Woo , Soon Il Kwon
Abstract: The present invention relates to method for preparing a plant from a protoplast comprising knocking-out or knocking-in one or more the endogenous gene of the protoplast, and the plant regenerated from a genome-modified protoplast prepared by the above method.
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3.发明申请A Method of Selecting a Nuclease Target Sequence for Gene Knockout Based on Microhomology 审中-公开基于微生物学选择基因敲除的核酸酶靶序列的方法公开(公告)号：US20170076039A1
公开(公告)日：2017-03-16
申请号：US15306270
申请日：2015-04-24
Applicant: INSTITUTE FOR BASIC SCIENCE
Inventor： Jin Soo Kim , Sang Su Bae
Abstract: The present invention relates to a method of selecting a nuclease target sequence for gene knockout based on microhomology.
Abstract translation: 本发明涉及一种基于微生物学选择基因敲除的核酸酶靶序列的方法。
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公开(公告)号：US11352666B2
公开(公告)日：2022-06-07
申请号：US15526528
申请日：2015-11-13
Applicant: INSTITUTE FOR BASIC SCIENCE
Inventor： Jin Soo Kim , Dae Sik Kim , Sang Su Bae
IPC: C12Q1/6874 , C12N9/22 , C12Q1/686 , C12Q1/6806 , G16B30/00 , G16B30/10
Abstract: The present disclosure relates to a method for detecting off-target sites of a programmable nuclease in a genome, and specifically, to a method for detecting off-target sites through data analysis by subjecting the genome isolated in vitro to programmable nucleases to cleave the genome and then performing whole genome sequencing or deep sequencing, and to a method for selecting on-target sites of a programmable nuclease, which minimizes the off-target effect, using this method. The Digenome-seq of the present disclosure can detect the off-target sites of a programmable nuclease on the genomic scale at a high degree of reproducibility, and thus can be used in the manufacture of programmable nucleases having high target specificity and the study thereof.
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公开(公告)号：US11149281B2
公开(公告)日：2021-10-19
申请号：US15766445
申请日：2016-10-06
Inventor： Jin Soo Kim , Jungeun Kim , Sunghwa Choe , Je Wook Woo , Soon Il Kwon , Hyeran Kim
Abstract: The present invention relates to a method of increasing the production efficiency of gene-edited plants, regenerated from plant protoplasts, by use of a Cas protein-guide RNA ribonucleoprotein (RNP). According to the present invention, the method of increasing the production efficiency of gene-edited plants makes it possible to efficiently produce target gene-mutated plants and to minimize the introduction of foreign DNA into plants. Thus, the present invention can be very advantageously used in a wide variety of fields, including agriculture, food and biotechnology.
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Patent Agency Ranking
公开(公告)号：US20170191123A1
公开(公告)日：2017-07-06
申请号：US15314288
申请日：2015-05-28
Applicant: TOOLGEN INCORPORATED , INSTITUTE FOR BASIC SCIENCE
Inventor： Jin Soo Kim , So Jung Kim , Seung Hwan Lee , Seok Joong Kim
IPC: C12Q1/68
CPC classification number: C12Q1/6844 , C12Q1/68 , C12Q2521/301 , G01N33/53 , G01N33/574
Abstract: The present invention relates to a method for analyzing a genotype using a target-specific nuclease and, specifically, to a method for diagnosing cancer or analyzing a genotype by removing wild type DNA or particular genotype DNA using a target-specific nuclease or a variant thereof to amplify or concentrate only a small amount of DNA which has a difference in variation, such as a mutation, or genotype, and to a method for separating target DNA sesusing a target-specific nuclease or a variant thereof. Such methods are novel paradigm methods contrary to existing simple target-specific nucleases for post-PCR recognition of normal genotype and carcinogenic genotype, and can be favorably used in the early diagnosis of cancer or analysis of similar genotypes.
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公开(公告)号：US11197935B2
公开(公告)日：2021-12-14
申请号：US15807850
申请日：2017-11-09
Applicant: INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY , INSTITUTE FOR BASIC SCIENCE
Inventor： Dong Wook Kim , Jin Soo Kim , Chul Yong Park , Duk Hyoung Kim , Jung Eun Kim , Jiyeon Kweon
IPC: A61K48/00 , C12N5/074 , C12N9/22 , C12N15/11 , A61K35/545 , C07K14/755 , C12N15/113 , A61K35/12
Abstract: The present invention provides a method for inducing an inversion of normal blood coagulation factor VIII (F8) gene, a method for correcting an inversion of blood coagulation factor VIII gene in which the inversion has occurred, and a Hemophilia A patient-derived induced pluripotent stem cell in which the inversion is corrected, constructed using the same. The method of the present invention effectively reproduces the inversion of intron 1 and intron 22 of the F8 gene, which is responsible for the majority of severe hemophilia A, and thereby may be effectively used for studying the development mechanism of hemophilia A and as a research tool for screening therapeutic agents. The inversion-corrected induced pluripotent stem cell constructed according the method of the present invention enables an efficient and fundamental treatment for hemophilia A by restoring a genotype in which mutation has occurred to a wild type-like state, without limitation via normal gene or protein delivery.
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公开(公告)号：US10858662B2
公开(公告)日：2020-12-08
申请号：US15527837
申请日：2015-11-19
Applicant: INSTITUTE FOR BASIC SCIENCE
Inventor： Jin Soo Kim , Tae Young Koo
IPC: C12N15/74 , C12N15/63 , C12N15/11 , C12N15/864 , C12N15/867 , C12N9/16 , C12N9/22
Abstract: The present invention relates to a method for regulating gene expression, comprising introducing into a cell each of a recombinant vector which expresses a first domain comprising N-terminus of a Cas9 protein, and a recombinant vector which expresses a second domain comprising C-terminus of a Cas9 protein, a composition comprising the recombinant vectors, a kit for regulating gene expression, and a method for intracellular production of Cas9 protein. Moreover, the present invention relates to a transformed cell introduced with a viral vector which packages the first domain, and a viral vector which packages the second domain, and to a composition comprising a virus produced therefrom.
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公开(公告)号：US10280402B2
公开(公告)日：2019-05-07
申请号：US15328628
申请日：2015-08-06
Applicant: College of Medicine Pochon CHA University Industry-Academic Cooperation Foundation , INSTITUTE FOR BASIC SCIENCE
Inventor： Jin Soo Kim , Dong Youn Hwang
IPC: C12N5/074 , C12Q1/6881 , C12N5/0735 , C07K14/74 , C12N5/073
Abstract: The present invention relates to a method for producing immune-compatible cells or a cell population which comprises a step of editing one or two alleles of one or more immune-compatible antigen genes by gene deletion or modification in an isolated cell comprising at least one of the immune-compatible antigen genes selected from HLA (human leukocyte antigen)-A, HLA-B and HLA-DR, to immune-compatible cells produced by the method, and to a cell population comprising the immune-compatible cells produced by the method.
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公开(公告)号：US20170349905A1
公开(公告)日：2017-12-07
申请号：US15527837
申请日：2015-11-19
Applicant: INSTITUTE FOR BASIC SCIENCE
Inventor： Jin Soo Kim , Tae Young Koo
IPC: C12N15/74 , C12N15/864 , C12N15/11 , C12N15/867 , C12N9/22
CPC classification number: C12N15/746 , C12N9/16 , C12N9/22 , C12N15/11 , C12N15/63 , C12N15/8645 , C12N15/867
Abstract: The present invention relates to a method for regulating gene expression, comprising introducing into a cell each of a recombinant vector which expresses a first domain comprising N-terminus of a Cas9 protein, and a recombinant vector which expresses a second domain comprising C-terminus of a Cas9 protein, a composition comprising the recombinant vectors, a kit for regulating gene expression, and a method for intracellular production of Cas9 protein. Moreover, the present invention relates to a transformed cell introduced with a viral vector which packages the first domain, and a viral vector which packages the second domain, and to a composition comprising a virus produced therefrom.
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