公开(公告)号：US20210032621A1

公开(公告)日：2021-02-04

申请号：US16964277

申请日：2019-01-23

Applicant: INSTITUTE FOR BASIC SCIENCE

Inventor： Jin-Soo KIM ,  Ka Yeong LIM ,  Beum-Chang KANG ,  Seuk Min RYU

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/90

Abstract: The present disclosure relates to: an extended guide RNA and a composition for base editing, comprising the same; and a method for base editing and a method for producing genetically modified animals or plants, both methods using the composition for base editing.

公开(公告)号：US20170314016A1

公开(公告)日：2017-11-02

申请号：US15347871

申请日：2016-11-10

Applicant: INSTITUTE FOR BASIC SCIENCE

Inventor： Jin-Soo KIM ,  So Jung KIM

IPC: C12N15/11 ,  A61K48/00 ,  C12N9/22 ,  C12P19/34 ,  C12N15/10 ,  C12N2310/20

CPC classification number: C12N15/11 ,  A61K48/005 ,  C12N9/22 ,  C12N15/1024 ,  C12N15/111 ,  C12N15/63 ,  C12N2310/20 ,  C12N2310/31 ,  C12N2320/32 ,  C12N2320/53 ,  C12N2330/30 ,  C12P19/34

Abstract: Provided are a composition for ribonucleoprotein delivery, comprising a guide RNA free of 5′-terminal phosphates, and a method for ribonucleoprotein delivery, using the same.

公开(公告)号：US20180078620A1

公开(公告)日：2018-03-22

申请号：US15661300

申请日：2017-07-27

Applicant: INSTITUTE FOR BASIC SCIENCE ,  SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION ,  SEOUL NATIONAL UNIVERSITY HOSPITAL

Inventor： Jin-Soo KIM ,  Jeong Hun KIM ,  Sung Wook PARK ,  Kyoungmi KIM

IPC: A61K38/46 ,  C12N15/11

CPC classification number: A61K38/465 ,  A61K48/00 ,  C12N15/102 ,  C12N15/11 ,  C12N15/1136 ,  C12N2310/20 ,  A61K2300/00

Abstract: Provided are a method of preventing and/or treating an eye disease, using a Cas9 protein and a guide RNA targeting VEGF-A, and a ribonucleoprotein including a Cas9 protein and a guide RNA targeting VEGF-A.

公开(公告)号：US20230151343A1

公开(公告)日：2023-05-18

申请号：US17919588

申请日：2021-04-26

Applicant: INSTITUTE FOR BASIC SCIENCE

Inventor： Jin-Soo KIM ,  Kayeong LIM ,  Jaesuk LEE

IPC: C12N9/22

CPC classification number: C12N9/22

Abstract: The present invention relates to a Cas9 variant or a nucleic acid encoding the same, a composition for editing a genome using Cas9 or a Cas9 variant or a nucleic acid encoding the same, and a method of editing a genome using the composition. Specifically, the present invention relates to a composition for editing a genome with excellent efficiency while reducing unwanted insertions/deletions (indels) by using a prime editing nuclease or a variant thereof, for example, Cas9 or a Cas9 variant or a nucleic acid encoding the same, and a method of editing a genome using the composition.

公开(公告)号：US20240294945A1

公开(公告)日：2024-09-05

申请号：US18025446

申请日：2021-09-17

Applicant: INSTITUTE FOR BASIC SCIENCE

Inventor： Jin-Soo KIM ,  Kayeong LIM ,  Sung lk CHO ,  Beum-Chang KANG ,  Seonghyun LEE ,  Hyunji LEE ,  Young Geun MOK ,  Ji Min LEE ,  Eugene CHUNG

IPC: C12N15/90 ,  A61K48/00 ,  C12N9/22 ,  C12N9/78 ,  C12N15/10 ,  C12N15/82

CPC classification number: C12N15/90 ,  A61K48/005 ,  C12N9/22 ,  C12N9/78 ,  C12N15/102 ,  C12N15/82

Abstract: The present invention relates to a cytosine or adenine deaminase in an isolated form or a variant thereof, a non-toxic full-length cytosine deaminase or a variant thereof, a fusion protein comprising same, a composition for base editing, and a method for editing a base by using same.

公开(公告)号：US20240018492A1

公开(公告)日：2024-01-18

申请号：US18247108

申请日：2021-09-29

Applicant: INSTITUTE FOR BASIC SCIENCE

Inventor： Jin-Soo KIM ,  Chul-Yong PARK

IPC: C12N9/22 ,  C12N15/10 ,  C12N15/63 ,  C12N9/12

CPC classification number: C12N9/22 ,  C12N15/102 ,  C12N15/63 ,  C12N9/1276 ,  C12N2310/20 ,  C12Y207/07049

Abstract: The present invention relates to: a prime editing composition comprising a prime editor protein or a nucleic acid encoding same, and a prime editing guide RNA (pegRNA); and a prime editing method.

公开(公告)号：US20180258418A1

公开(公告)日：2018-09-13

申请号：US15872907

申请日：2018-01-16

Applicant: INSTITUTE FOR BASIC SCIENCE

Inventor： Jin-Soo KIM

IPC: C12N15/10 ,  C12N9/22 ,  C12N15/90 ,  C12N15/113

Abstract: Provided are a composition for inducing DNA single strand breaks in DNA, the composition comprising a cytidine deaminase, an inactivated target-specific endonuclease, and a guide RNA, a method for inducing a single-strand break in DNA, using the same, a method for analyzing a nucleic acid sequence of a base-editing-introduced DNA, and a method for identifying (or measuring or detecting) a base-editing site, base-editing efficiency at an on-target site, an off-target site, and/or target specificity.