公开(公告)号：US20190233857A1

公开(公告)日：2019-08-01

申请号：US16264774

申请日：2019-02-01

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Daniel Bawdon ,  Mariusz Stanislaw Kamionka ,  Alexander Brett Foster ,  Arghya Barman

IPC: C12P13/06 ,  C12N15/74 ,  C12N9/04 ,  C12N9/16 ,  C12N9/10

CPC classification number: C12P13/06 ,  C12N9/0006 ,  C12N9/1096 ,  C12N9/16 ,  C12N15/74 ,  C12Y101/01095 ,  C12Y206/01052 ,  C12Y301/03003

Abstract: Biosynthetic methods and materials for the production of compounds involved in serine metabolism, derivatives thereof and/or compounds related thereto are provided. Also provided are products produced in accordance with these methods and materials.

公开(公告)号：US20190352674A1

公开(公告)日：2019-11-21

申请号：US16399145

申请日：2019-04-30

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alexander Brett FOSTER ,  Cristina Serrano AMATRIAIN ,  Gary J. Smith ,  Paul Sheldon Pearlman ,  Mark Paul Taylor ,  Jonathan Combe ,  Daniel Bawdon

IPC: C12P7/04 ,  C12N1/20 ,  C12P7/06 ,  C12P7/42 ,  C12N9/10 ,  C12N9/88 ,  C12N9/04

Abstract: Provided herein are methods for increasing the yield of an extracellular product synthesized by an organism cultured in a continuous aerobic fermentation system. The extracellular product yield is increased through the use of an organism modified to decreased production of polyhydroxyalkanoate, to increase production of the extracellular product, and to include promoters that can be inducible in response to nutrient limitation conditions. The extracellular product yield is also increased by operating the continuous fermentation system under particular nutrient limitation conditions. Also provided are non-naturally occurring organisms that have been modified for use with the provided methods, and extracellular products made using the provided methods.

公开(公告)号：US20180100160A1

公开(公告)日：2018-04-12

申请号：US15717216

申请日：2017-09-27

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Daniel Bawdon ,  Stephen Thomas Cartman ,  Jonathan Kennedy

IPC: C12N15/74 ,  C12N15/64 ,  C12N13/00

CPC classification number: C12N15/74 ,  C12N9/22 ,  C12N13/00 ,  C12N15/64

Abstract: Nonnaturally occurring host cells altered to increase their ability to transfer genetic molecules into the host cells as compared to an unaltered host cell are provided. Also provided are methods for identifying endogenous loci of a host cell which inhibit transformation efficiency and/or electroporation of genetic molecules into the cell as well as methods for producing nonnaturally occurring host cells with enhanced transformation efficiency and/or the modified ability to allow for genomic integration of an exogenous DNA sequence via electroporation. Methods for producing biochemicals and products produced with the nonnaturally occurring host cells are also provided.

公开(公告)号：US20190284564A1

公开(公告)日：2019-09-19

申请号：US16337491

申请日：2017-09-27

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Daniel Bawdon ,  Stephen Thomas Cartman ,  Jonathan Kennedy

IPC: C12N15/52 ,  C12N15/74 ,  C12N9/22

Abstract: Nonnaturally occurring host cells altered to increase their ability to transfer genetic molecules into the host cells as compared to an unaltered host cell are provided. Also provided are methods for identifying endogenous loci of a host cell which inhibit transformation efficiency and/or electroporation of genetic molecules into the cell as well as methods for producing nonnaturally occurring host cells with enhanced transformation efficiency and/or the modified ability to allow for genomic integration of an exogenous DNA sequence via electroporation. Methods for producing biochemicals and products produced with the nonnaturally occurring host cells are also provided. In particular, a host Cupriavidus necator cell is provided in which a chromosomal gene encoding a restriction endonuclease has been disrupted, leading to an increase in transformation efficiency.