公开(公告)号：US20190338376A1

公开(公告)日：2019-11-07

申请号：US16398384

申请日：2019-04-30

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alexander Brett Foster ,  Jonathan Kennedy

IPC: C12R1/38 ,  C12N15/78 ,  C12N15/52 ,  C12P7/04

Abstract: Methods for increasing carbon-based chemical product yield in an organism by genetically modifying one or more genes involved in a stringent response and/or in a regulatory network, nonnaturally occurring organisms having increased carbon-based chemical product yield, and methods for use in production of carbon-based chemical products are provided.

公开(公告)号：US10801046B2

公开(公告)日：2020-10-13

申请号：US15659505

申请日：2017-07-25

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alexander Brett Foster ,  Stephen Thomas Cartman ,  Jonathan Kennedy ,  William Joseph Simmons

IPC: C12P7/62 ,  C12P7/42 ,  C12N15/09 ,  C12P7/64 ,  C12N15/52 ,  C12P13/00 ,  C12P7/24 ,  C12P7/40 ,  C07C47/12 ,  C07C47/19 ,  C07C55/16 ,  C07C59/147 ,  C07C223/02 ,  C07C225/06 ,  C07C229/08 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12P7/18 ,  C12P7/44

Abstract: This document describes biochemical pathways for producing a difunctional product having an odd number of carbon atoms in vitro or in a recombinant host, or salts or derivatives thereof, by forming two terminal functional groups selected from carboxyl, amine, formyl, and hydroxyl groups in an aliphatic carbon chain backbone having an odd number of carbon atoms synthesized from (i) acetyl-CoA and propanedioyl-CoA via one or more cycles of methyl ester shielded carbon chain elongation or (ii) propanedioyl-[acp] via one or more cycles of methyl ester shielded carbon chain elongation. The biochemical pathways and metabolic engineering and cultivation strategies described herein rely on enzymes or homologs accepting methyl ester shielded aliphatic carbon chain backbones and maintaining the methyl ester shield for at least one further enzymatic step following one or more cycles of methyl ester shielded carbon chain elongation.

公开(公告)号：US20180100160A1

公开(公告)日：2018-04-12

申请号：US15717216

申请日：2017-09-27

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Daniel Bawdon ,  Stephen Thomas Cartman ,  Jonathan Kennedy

IPC: C12N15/74 ,  C12N15/64 ,  C12N13/00

CPC classification number: C12N15/74 ,  C12N9/22 ,  C12N13/00 ,  C12N15/64

Abstract: Nonnaturally occurring host cells altered to increase their ability to transfer genetic molecules into the host cells as compared to an unaltered host cell are provided. Also provided are methods for identifying endogenous loci of a host cell which inhibit transformation efficiency and/or electroporation of genetic molecules into the cell as well as methods for producing nonnaturally occurring host cells with enhanced transformation efficiency and/or the modified ability to allow for genomic integration of an exogenous DNA sequence via electroporation. Methods for producing biochemicals and products produced with the nonnaturally occurring host cells are also provided.

公开(公告)号：US20180023104A1

公开(公告)日：2018-01-25

申请号：US15656482

申请日：2017-07-21

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Stephen Cartman ,  Jonathan Combe ,  Alexander B. Foster ,  Jonathan Kennedy

IPC: C12P13/00 ,  C08G69/10 ,  C12N9/10 ,  C12N9/04 ,  C12N9/02 ,  C12N15/52

CPC classification number: C12P13/005 ,  C08G69/08 ,  C08G69/10 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N15/52 ,  C12Y101/01021 ,  C12Y102/01003 ,  C12Y102/99006 ,  C12Y203/01009 ,  C12Y206/01

Abstract: This disclosure relates to genome-scale attenuation or knockout strategies for directing carbon flux to certain carbon based building blocks within the 7-aminoheptanoic acid (7-AHA) and 6-aminohexanoic acid (6-AHA) biosynthesis pathways, for example, to achieve reduced flux to unwanted side products while achieving increased production of desired intermediates and end products. This disclosure also relates to non-naturally occurring mutant bacterial strains comprising one or more gene disruptions in aldehyde reductase and/or aldehyde dehydrogenase genes that are generated to direct carbon flux to certain carbon based building blocks. This disclosure further relates to a method for enhancing production of carbon based building blocks by generating non-naturally occurring mutant bacterial strains, culturing said mutant bacterial strains in the presence of suitable substrates or under desired growth conditions, and substantially purifying the desired end product.

公开(公告)号：US20190352682A1

公开(公告)日：2019-11-21

申请号：US16372083

申请日：2019-04-01

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alexander Brett Foster ,  Jonathan Kennedy ,  Paul S. Pearlman

IPC: C12P13/00 ,  C12N9/10

Abstract: This disclosure relates to strategies for in vivo production of certain carbon-based products, for example, aminated aliphatic compounds having a carbon chain length of C5-C19.

公开(公告)号：US20190284564A1

公开(公告)日：2019-09-19

申请号：US16337491

申请日：2017-09-27

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Daniel Bawdon ,  Stephen Thomas Cartman ,  Jonathan Kennedy

IPC: C12N15/52 ,  C12N15/74 ,  C12N9/22

Abstract: Nonnaturally occurring host cells altered to increase their ability to transfer genetic molecules into the host cells as compared to an unaltered host cell are provided. Also provided are methods for identifying endogenous loci of a host cell which inhibit transformation efficiency and/or electroporation of genetic molecules into the cell as well as methods for producing nonnaturally occurring host cells with enhanced transformation efficiency and/or the modified ability to allow for genomic integration of an exogenous DNA sequence via electroporation. Methods for producing biochemicals and products produced with the nonnaturally occurring host cells are also provided. In particular, a host Cupriavidus necator cell is provided in which a chromosomal gene encoding a restriction endonuclease has been disrupted, leading to an increase in transformation efficiency.

公开(公告)号：US10889839B2

公开(公告)日：2021-01-12

申请号：US16685290

申请日：2019-11-15

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alexander Steinbüchel ,  Jessica Eggers ,  Alexander Brett Foster ,  Jonathan Kennedy

IPC: C12P7/16 ,  C12R1/01 ,  C12N1/20

Abstract: Nonnaturally occurring organisms exhibiting improved carbon utilization and methods for production and use of these nonnaturally occurring organisms in chemical production from carbon containing feedstocks are provided.