公开(公告)号：JP2008083047A

公开(公告)日：2008-04-10

申请号：JP2007243822

申请日：2007-09-20

Applicant: Leica Microsystems Cms Gmbh ,  ライカ マイクロシステムス ツェーエムエス ゲーエムベーハー

Inventor： KNEBEL WERNER ,  SCHROEDER JAN

IPC: G01N21/78 ,  C12Q1/00 ,  C12Q1/34 ,  G01N21/64 ,  G01N33/533 ,  G01N33/58

CPC classification number: G01N33/542

Abstract: PROBLEM TO BE SOLVED: To provide a method for examining a structure that is labeled, at high spatial resolution, by fluorogenic material of a specimen. SOLUTION: The method for examining the structure that is labeled, at a high spatial resolution, by a fluorogenic material of the specimen and that can repeatedly convert the fluorogenic material, from a first state into a second that are mutually different states regarding at least one optical characteristic includes a step of initially setting a substance to be detected within a specimen region in the first state; and a step of inducing the second state, in a manner of controlling small regions that are spatially divided by an optical signal within the specimen region. A ligand complex (1), containing the fluorogenic material, is bonded to an enzyme (4) through an enzyme reaction inside a cell; and based on the fact that the enzyme (4) is developed as a fusion protein (6), together with a target protein (5), the protein within the living cell i.e. the target protein (5) is defined by being used as a structure labeled by the fluorogenic material. COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供一种用于检查以高空间分辨率由样品的荧光材料标记的结构的方法。 解决方案：用于检查以高空间分辨率由样品的荧光材料标记并且可以将荧光材料重复转换的第一状态的第二状态的结构的方法，所述第二状态是相互不同的状态 至少一个光学特性包括在第一状态下在样本区域内初始设定待检测物质的步骤; 以及以控制由样本区域内的光信号空间分割的小区域的方式诱导第二状态的步骤。 含有荧光物质的配体络合物（1）通过细胞内的酶反应与酶（4）结合; 并且基于酶（4）作为融合蛋白（6）与靶蛋白（5）一起发展的事实，活细胞内的蛋白质即靶蛋白（5）被定义为作为 由荧光材料标记的结构。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2006011440A

公开(公告)日：2006-01-12

申请号：JP2005182095

申请日：2005-06-22

Applicant: Leica Microsystems Cms Gmbh ,  ライカ ミクロジュステムス ツェーエムエス ゲーエムベーハー

Inventor： SEYFRIED VOLKER ,  KNEBEL WERNER ,  SCHROEDER JAN

IPC: G02B21/00 ,  G01N21/64 ,  G02B21/06 ,  G02B21/16 ,  G02F1/13 ,  G02F1/33

CPC classification number: G02B21/16

Abstract: PROBLEM TO BE SOLVED: To provide a microscope of the kind with which interference-free image acquisition is possible and negative impact on the detector is avoided, even in the presence of critical measurement parameters. SOLUTION: The microscope includes at least one illuminating light source, illumination beam path for guiding the illuminating light to a specimen, a detector, and a detection beam path for guiding detected light from the specimen to the detector. In such a microscope, in particular a fluorescence microscope, an activatable element is positioned in the detection beam path (3) for regulating and/or limiting power level in the detection beam path (3). COPYRIGHT: (C)2006,JPO&NCIPI

Abstract translation: 要解决的问题：即使在存在关键测量参数的情况下，提供可以避免无干扰图像获取的类型的显微镜并避免对检测器的负面影响。 解决方案：显微镜包括至少一个照明光源，用于将照射光引导到样本的照明光束路径，检测器和用于将检测到的光从样本引导到检测器的检测光束路径。 在这种显微镜中，特别是荧光显微镜，可检测光束路径（3）中的可激活元件位于检测光束路径（3）中用于调节和/或限制功率电平。 版权所有（C）2006，JPO＆NCIPI

公开(公告)号：DE102004032952A1

公开(公告)日：2006-01-26

申请号：DE102004032952

申请日：2004-07-07

Applicant: LEICA MICROSYSTEMS

Inventor： KNEBEL WERNER ,  SCHROEDER JAN

IPC: G01N21/64 ,  G02B21/00

Abstract: Disclosed is a method for analyzing biological samples by means of a scanning microscope. According to said method, at least one screen dot is repeatedly and successively illuminated with a manipulating light beam and an exciting light beam. The interval between the time of illumination with the manipulating light beam and the time of illumination with the exciting light beam is modified, and the fluorescent light yield is measured in accordance with said interval.

公开(公告)号：DE102006045607A1

公开(公告)日：2008-03-27

申请号：DE102006045607

申请日：2006-09-25

Applicant: LEICA MICROSYSTEMS

Inventor： KNEBEL WERNER ,  SCHROEDER JAN

IPC: G02B21/00 ,  G01N21/64

Abstract: The research process involves using a target protein (5) in living cells as the structure which is marked with the fluorescent substance (3). A ligand complex (1) including the fluorescent substance is bonded to and enzyme (4) by an enzymatic reaction in the cell. The enzyme is expelled as a fusion protein (6) together with the target protein.

公开(公告)号：DE102004031048A1

公开(公告)日：2006-01-12

申请号：DE102004031048

申请日：2004-06-25

Applicant: LEICA MICROSYSTEMS

Inventor： KNEBEL WERNER ,  SEYFRIED VOLKER ,  SCHROEDER JAN

IPC: G02B21/00 ,  G02B21/06 ,  G02B21/16

Abstract: A microscope includes at least one illuminating light source and an illumination beam path for guiding the illuminating light to a specimen. A detection beam path guides detected light from the specimen to a detector. An activatable element is positioned in the detection beam path for regulating and/or limiting the light power level in the detection beam path.