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1.发明申请
公开(公告)号：WO2017013567A1
公开(公告)日：2017-01-26
申请号：PCT/IB2016/054260
申请日：2016-07-18
Applicant: THE CATHOLIC UNIVERSITY OF AMERICA
Inventor： RAO, Venigalla B. , ALSALMI, Wadad
IPC: C12N15/63 , C12P21/00 , C07K14/005
CPC classification number: C07K14/005 , A61K9/7023 , A61K39/00 , A61K39/12 , A61K39/21 , A61K2039/54 , A61K2039/627 , C07K1/22 , C07K1/36 , C07K14/162 , C07K2319/00 , C07K2319/20 , C07K2319/21 , C07K2319/22 , C07K2319/40 , C07K2319/50 , C12N7/00 , C12N2740/16022 , C12N2740/16043 , C12N2740/16051 , C12N2740/16111 , C12N2740/16122 , C12N2740/16134 , C12N2740/16234
Abstract: An approach of producing recombinant trimers that mimic native HIV-1 envelope trimers is developed. A recombinant protein forming the recombinant trimers encompasses a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gp140. The linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix. After expressed in a cell, the recombinant protein is secreted into the culture medium and assembles into recombinant trimers therein. The recombinant trimers may be directly purified from the culture medium. Cleaved and uncleaved trimers from different clade viruses are produced.
Abstract translation: 开发了生产模仿天然HIV-1包膜三聚体的重组三聚体的方法。 形成重组三聚体的重组蛋白包含通过重组HIV-1 gp140的C-末端的接头与标签融合的重组HIV-1 gp140。 接头足够长，使得标签可通过结合在固体基质上的结合分子进行结合。 在细胞中表达后，将重组蛋白分泌到培养基中并在其中组装成重组三聚体。 重组三聚体可以从培养基中直接纯化。 产生来自不同进化枝病毒的切割和未切割的三聚体。
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2.发明申请
公开(公告)号：WO2022130196A1
公开(公告)日：2022-06-23
申请号：PCT/IB2021/061688
申请日：2021-12-14
Applicant: THE CATHOLIC UNIVERSITY OF AMERICA
Inventor： RAO, Venigalla B. , ZHU, Jingen
IPC: C12N7/00 , C12N9/22 , C07K14/005 , A61K39/215 , C12N15/70
Abstract: The present disclosure relates to a system for and a method of incorporating SARS-CoV-2 genes and proteins into T4 phages. The present disclosure also relates to vaccine against SARS-CoV-2 containing recombinant T4 phages created using the method provided in the present disclosure.
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公开(公告)号：WO2022023850A1
公开(公告)日：2022-02-03
申请号：PCT/IB2021/056248
申请日：2021-07-21
Applicant: THE CATHOLIC UNIVERSITY OF AMERICA
Inventor： RAO, Venigalla B. , ZHU, Jingen
IPC: C12N15/86 , C12N15/113 , C12N9/22 , C12N15/10 , C12N15/88 , A61K48/00 , A61K31/7088 , C12N2795/00043 , C12N2795/00051 , C12N7/00
Abstract: Described is an "artificial virus" (AV) programmed with biomolecules that can enter human cells and carry out precise human genome modification. The AVs comprise: at least one viral vector, such as bacteriophage T4; at least one therapeutic molecule, such as DNA, RNA, protein and their complex; and a lipid coating. Also described is a method of human genome modification, using such an AV, and a method of program such an AV.
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4.发明申请
公开(公告)号：WO2021033082A1
公开(公告)日：2021-02-25
申请号：PCT/IB2020/057588
申请日：2020-08-12
Applicant: THE CATHOLIC UNIVERSITY OF AMERICA
Inventor： RAO, Venigalla B. , ZHU, Jingen
Abstract: Described is hybrid viral vector comprising: a first virus such as bacteriophage T4; one or more second virus such as adeno-associated virus (AAV) attached to the first virus through cross-bridges, such as avidin-biotin cross-bridges; one or more DNA molecules packaged in the first virus; one or more nucleic acid molecules packaged in the second virus; and one or more proteins displayed on the surface of the first virus. Also described are methods of making and using such a hybrid viral vector.
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5.发明申请
公开(公告)号：WO2017013564A1
公开(公告)日：2017-01-26
申请号：PCT/IB2016/054250
申请日：2016-07-15
Applicant: THE CATHOLIC UNIVERSITY OF AMERICA
Inventor： RAO, Venigalla B. , ALSALMI, Wadad
IPC: C07K14/005 , C12N15/63
CPC classification number: C07K14/005 , A61K9/7023 , A61K39/00 , A61K39/12 , A61K39/21 , A61K2039/54 , A61K2039/627 , C07K1/22 , C07K1/36 , C07K14/162 , C07K2319/00 , C07K2319/20 , C07K2319/21 , C07K2319/22 , C07K2319/40 , C07K2319/50 , C12N7/00 , C12N2740/16022 , C12N2740/16043 , C12N2740/16051 , C12N2740/16111 , C12N2740/16122 , C12N2740/16134 , C12N2740/16234
Abstract: An approach of producing recombinant trimers that mimic native HIV- 1 envelope trimers is developed. A recombinant protein forming the recombinant trimers encompasses a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV - 1 gp140. The linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix. After expressed in a cell, the recombinant protein is secreted into the culture medium and assembles into recombinant trimers therein. The recombinant trimers may be directly purified from the culture medium. Cleaved and uncleaved trimers from different clade viruses are produced.
Abstract translation: 开发了生产模仿天然HIV-1包膜三聚体的重组三聚体的方法。 形成重组三聚体的重组蛋白包含通过重组HIV-1 gp140的C-末端的接头与标签融合的重组HIV-1 gp140。 接头足够长，使得标签可通过结合在固体基质上的结合分子进行结合。 在细胞中表达后，将重组蛋白分泌到培养基中并在其中组装成重组三聚体。 重组三聚体可以从培养基中直接纯化。 产生来自不同进化枝病毒的切割和未切割的三聚体。
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Patent Agency Ranking6.发明申请MUTATED AND BACTERIOPHAGE T4 NANOPARTICLE ARRAYED F1-V IMMUNOGENS FROM YERSINA PESTIS AS NEXT GENERATION PLAGUE VACCINES 审中-公开MUTAT和BACTERIOPHAGE T4 NANOPARTICLE将来自YERSINA PESTIS的F1-V免疫调节剂作为下一代生产疫苗
公开(公告)号：WO2015004627A1
公开(公告)日：2015-01-15
申请号：PCT/IB2014/063005
申请日：2014-07-10
Applicant: THE CATHOLIC UNIVERSITY OF AMERICA
Inventor： RAO, Venigalla B. , TAO, Pan
CPC classification number: C07K14/24 , A61K39/0291 , A61K2039/5256 , C07K14/005 , C07K2319/21 , C07K2319/40 , C07K2319/735 , C12N2795/10123 , Y02A50/407
Abstract: Techniques from two basic approaches, structure-based immunogen design and phage T4 nanoparticle delivery, are developed to construct new plague vaccines. The NH 2 - terminal β-strand of F1 of Yersinia pestis is transplanted to the COOH-terminus of F1 of Yersinia pestis and the NH 2 -- terminus sequence flanking the β-strand of F1 of Yersinia pestis is duplicated to eliminate polymerization but to retain the T cell epitopes. The mutated F1 is fused to the V antigen of Yersinia pestis to thereby form a fusion protein F1mut-V mutant, which produces a completely soluble monomer. The fusion protein F1mut-V is then arrayed on phage T4 nanoparticles via a small outer capsid protein, Soc, from a T4 phage or a T4-related phage. Both the soluble and T4 decorated F1mut-V provided approximately 100% protection to mice and rats against pneumonic plague evoked by high doses of Yersinia pestis CO92.
Abstract translation: 开发了两种基本方法，基于结构的免疫原设计和噬菌体T4纳米颗粒递送的技术来构建新的疫苗疫苗。 将鼠疫耶尔森氏菌的F1的NH2-末端菌株移植到鼠疫耶尔森氏菌的F1的COOH-末端，并且重复鼠疫耶尔森氏菌的F1侧链的NH 2 - 末端序列，以消除聚合反应 保留T细胞表位。 突变的F1融合到鼠疫耶尔森氏菌的V抗原，从而形成融合蛋白F1mut-V突变体，其产生完全可溶的单体。 然后通过T4噬菌体或与T4相关的噬菌体的小外壳衣壳蛋白Soc，将融合蛋白F1mut-V排列在噬菌体T4纳米颗粒上。 可溶性和T4装饰的F1mut-V都能够对小鼠和大鼠提供大约100％的抗高剂量鼠疫耶尔森氏菌CO92诱发的肺炎瘟疫。
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7.发明申请
公开(公告)号：WO2023285922A1
公开(公告)日：2023-01-19
申请号：PCT/IB2022/056234
申请日：2022-07-06
Applicant: THE CATHOLIC UNIVERSITY OF AMERICA
Inventor： RAO, Venigalla B. , BATRA, Himanshu
IPC: C12N15/86 , C07K14/005 , C12N7/00 , C12N5/0783
Abstract: Described is an engineered viral particle programmed with T cell targeting specificity. The viral particles comprise: at least one viral vector, such as bacteriophage T4; and at least one CD4-binding protein displayed on the surface of the viral vector. Also described is a method of reactivate latent HIV-1 and cure patient with HIV-1 infection, using such an engineered viral particle.
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8.发明申请
公开(公告)号：WO2017013584A1
公开(公告)日：2017-01-26
申请号：PCT/IB2016/054292
申请日：2016-07-19
Applicant: THE CATHOLIC UNIVERSITY OF AMERICA
Inventor： RAO, Venigalla B. , ALSALMI, Wadad
IPC: A61K39/395 , A61K39/12 , A61K39/00
CPC classification number: C07K14/005 , A61K9/7023 , A61K39/00 , A61K39/12 , A61K39/21 , A61K2039/54 , A61K2039/627 , C07K1/22 , C07K1/36 , C07K14/162 , C07K2319/00 , C07K2319/20 , C07K2319/21 , C07K2319/22 , C07K2319/40 , C07K2319/50 , C12N7/00 , C12N2740/16022 , C12N2740/16043 , C12N2740/16051 , C12N2740/16111 , C12N2740/16122 , C12N2740/16134 , C12N2740/16234
Abstract: Provided herein are HIV vaccines that encompasses recombinant trimers that mimic native HIV-1 envelope trimers. Also provided are methods of administering to a subject in need thereof an HIV vaccine provided herein to elicit antibodies against a recombinant trimer in the subject. A recombinant trimer is formed by a recombinant protein comprising a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gp140, wherein the linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix during purification of the recombinant trimer.
Abstract translation: 本文提供涵盖模仿天然HIV-1包膜三聚体的重组三聚体的HIV疫苗。 还提供了向有需要的受试者施用本文提供的HIV疫苗以引发针对受试者中重组三聚体的抗体的方法。 重组三聚体由包含重组HIV-1 gp140的重组蛋白形成，重组HIV-1 gp140通过重组HIV-1 gp140的C-末端的接头与标签融合，其中接头足够长，使得标签可通过 在纯化重组三聚体期间结合在固体基质上的结合分子。
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9.发明申请
公开(公告)号：WO2017013545A1
公开(公告)日：2017-01-26
申请号：PCT/IB2016/054207
申请日：2016-07-14
Applicant: THE CATHOLIC UNIVERSITY OF AMERICA
Inventor： RAO, Venigalla B. , ALSALMI, Wadad
IPC: C07K14/005 , C07K1/14 , C07K1/16 , C07K1/22 , C07K1/36
CPC classification number: C07K14/005 , A61K9/7023 , A61K39/00 , A61K39/12 , A61K39/21 , A61K2039/54 , A61K2039/627 , C07K1/22 , C07K1/36 , C07K14/162 , C07K2319/00 , C07K2319/20 , C07K2319/21 , C07K2319/22 , C07K2319/40 , C07K2319/50 , C12N7/00 , C12N2740/16022 , C12N2740/16043 , C12N2740/16051 , C12N2740/16111 , C12N2740/16122 , C12N2740/16134 , C12N2740/16234
Abstract: An approach of producing recombinant trimers that mimic native HIV-1 envelope trimers is developed. A recombinant protein forming the recombinant trimers encompasses a recombinant HIV-1 gpl40 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gpl40. The linker is sufficiently long so that the tag is accessible for binding by a binding molecule bound on a solid matrix. After expressed in a cell, the recombinant protein is secreted into the culture medium and assembles into recombinant trimers therein. The recombinant trimers may be directly purified from the culture medium. Cleaved and uncleaved trimers from different clade viruses are produced.
Abstract translation: 开发了生产模仿天然HIV-1包膜三聚体的重组三聚体的方法。 形成重组三聚体的重组蛋白包含通过重组HIV-1 gp120的C末端的接头与标签融合的重组HIV-1 gp140。 接头足够长，使得标签可通过结合在固体基质上的结合分子进行结合。 在细胞中表达后，将重组蛋白分泌到培养基中并在其中组装成重组三聚体。 重组三聚体可以从培养基中直接纯化。 产生来自不同进化枝病毒的切割和未切割的三聚体。
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10.发明申请
公开(公告)号：WO2017013537A1
公开(公告)日：2017-01-26
申请号：PCT/IB2016/054176
申请日：2016-07-13
Applicant: THE CATHOLIC UNIVERSITY OF AMERICA
Inventor： RAO, Venigalla B. , ALSALMI, Wadad
IPC: C07K14/005
CPC classification number: C07K14/005 , A61K9/7023 , A61K39/00 , A61K39/12 , A61K39/21 , A61K2039/54 , A61K2039/627 , C07K1/22 , C07K1/36 , C07K14/162 , C07K2319/00 , C07K2319/20 , C07K2319/21 , C07K2319/22 , C07K2319/40 , C07K2319/50 , C12N7/00 , C12N2740/16022 , C12N2740/16043 , C12N2740/16051 , C12N2740/16111 , C12N2740/16122 , C12N2740/16134 , C12N2740/16234
Abstract: An approach of producing recombinant trimers that mimic native HIV-1 envelope trimers is developed. A recombinant protein forming the recombinant trimers encompasses a recombinant HIV-1 gp140 fused to a tag through a linker at C-terminus of the recombinant HIV-1 gp140. The linker is sufficiently long so that the tag is accessible for binding by a 5 binding molecule bound on a solid matrix. After expressed in a cell, the recombinant protein is secreted into the culture medium and assembles into recombinant trimers therein. The recombinant trimers may be directly purified from the culture medium. Cleaved and uncleaved trimers from different clade viruses are produced.
Abstract translation: 开发了生产模仿天然HIV-1包膜三聚体的重组三聚体的方法。 形成重组三聚体的重组蛋白包含通过重组HIV-1 gp140的C-末端的接头与标签融合的重组HIV-1 gp140。 接头足够长，使得标签可通过结合在固体基质上的5结合分子进行结合。 在细胞中表达后，将重组蛋白分泌到培养基中并在其中组装成重组三聚体。 重组三聚体可以从培养基中直接纯化。 产生来自不同进化枝病毒的切割和未切割的三聚体。
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