公开(公告)号：JPS6466558A

公开(公告)日：1989-03-13

申请号：JP22356887

申请日：1987-09-07

Applicant: TOA ELECTRONICS

Inventor： ISHII MASARU ,  KIYONO YUKO ,  GOTSU TOSHIO ,  AIKAWA KATSUAKI

IPC: G01N27/416 ,  G01N27/27 ,  G01N27/30

Abstract: PURPOSE:To obtain a high-level measuring accuracy, by having a fixed amount of ion species predetermined present in a reaction liquid to use an ion selecting electrode as reference electrode selectively responding to the ion species. CONSTITUTION:An immuno enzyme composite such as solid phase oriented antibody-antigen-enzyme labelled antibody is formed by immunological reaction and then, an enzyme reaction is caused between enzyme and a substrate of an immuno enzyme composite and the subsequent enzyme activity is measured with an oxidizing/reducing potential measuring apparatus. Here, somewhere between the outset of the immunological reaction and the measurement of an oxidizing/reducing potential, a fixed amount of specified ion species is made present in a reaction liquid. The reaction liquid 1 is sampled into a container 9 to be passed to a flow cell 2 having an indication electrode 3 and a reference electrode 4 selectively sensitive to the ion species with a peristatis pump 8 or the like to detect the oxidizing/reducing potential being discharged to a waste liquor tank 10. Then, a potential difference detected with both the electrodes 3 and 4 is shown as voltage value on a display section 7 through an amplifier 5 and a signal processing section 6 thereby obtaining a high-level measuring accuracy.

公开(公告)号：JPS63100378A

公开(公告)日：1988-05-02

申请号：JP24613986

申请日：1986-10-16

Applicant: NIPPON KAYAKU KK ,  TOA ELECTRONICS

Inventor： ISHII MASARU

IPC: G01N27/416 ,  C12Q1/00 ,  C12Q1/28 ,  G01N33/543

Abstract: PURPOSE:To permit easy measurement of enzyme activity over a wide measurable concn. range by using hydrogen peroxide electrodes to make electrochemical measurement of the concn. of hydrogen peroxide, thereby determining the enzyme activity. CONSTITUTION:The standard material in a standard liquid or the material to be measured in a sample and antibody converted to a solid phase is brought into reaction. After an unreacted material is removed, an enzyme labeling antibody is brought into reaction and again the unreacted labeling antibody is removed; thereafter, a substrate is added thereto to cause enzyme reaction. After the reaction is stopped, the reaction liquid is drawn in a specified amt. into a measuring cell 2 where the hydrogen peroxide electrodes 3 and 4 are installed. A diluting liquid of the amt. corresponding to the drawn reaction liquid itself or a needed amt. is added to said liquid, then the liquid is stirred and mixed. The current value is determined by the electrodes 3, 4 and is converted to a voltage value in a signal processing part 7. The value is displayed on a display part 8. Since the value thus obtd. is the value corresponding to the concn. of the hydrogen peroxide existing in the reaction liquid, the concn. of the component is determined from the voltage obtd. in such a manner.

公开(公告)号：JPS63265163A

公开(公告)日：1988-11-01

申请号：JP10068787

申请日：1987-04-23

Applicant: TOA ELECTRONICS ,  NIPPON KAYAKU KK

Inventor： ISHII MASARU ,  KIYONO YUKO ,  GOTSU TOSHIO ,  AIKAWA KATSUAKI

IPC: G01N33/543 ,  C12M1/34 ,  G01N27/416

Abstract: PURPOSE:To measure enzyme activity, by adding a substrate, which undergoes oxidation reduction reaction with oxygen, in a solution including prepared immunity enzyme composite, and detecting the oxidation reduction potential of the solution. CONSTITUTION:An immunity enzyme composite such as solidified antibody/antigen/enzyme marker antibody is formed by immunization reaction, and the enzyme reaction of the enzyme in the immunity enzyme composite and the substrate is made to occur. Then, a solution after the enzyme reaction is stopped is picked up and put in a measuring bath 1. With the solution being stirred with an agitator 2, a working electrode 3 and a reference electrode 4 are immersed in the solution and an oxidation reduction potential is detected. The potential difference detected with both electrodes 3 and 4 is displayed on a display part 7 as a voltage value through an amplifier 5 and a signal processing part 6. The obtained voltage value is the value corresponding to the concentration ratio between the reduced body and the oxidized body in the solution. The voltage value is obtained for a standard liquid with known concentration beforehand, and a calibration curve for relation between the concentration and the voltage value is prepared. Thus the concentration of the component is obtained from the voltage value which is obtained for a sample, according to the calibrating curve.