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公开(公告)号：WO2012013682A3
公开(公告)日：2012-05-18
申请号：PCT/EP2011062837
申请日：2011-07-26
Applicant: UCB PHARMA SA , SPITALI MARIANGELA , SYMMONS JONATHAN , WHITCOMBE RICHARD , PEARCE-HIGGINS MARK ROBERT
CPC classification number: C07K16/241 , C07K1/18 , C07K1/36 , C07K2317/14 , C07K2317/55
Abstract: The present invention relates to a process for the purification of an antibody fragment from a periplasmic cell extract comprising a first cation exchange chromatography step and a second anion exchange chromatography step.
Abstract translation: 本发明涉及从周质细胞提取物纯化抗体片段的方法，其包括第一阳离子交换层析步骤和第二阴离子交换层析步骤。
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公开(公告)号：AU2011284896B2
公开(公告)日：2016-06-16
申请号：AU2011284896
申请日：2011-07-26
Applicant: UCB PHARMA SA
Abstract: The present invention relates to a process for the purification of an antibody fragment from a periplasmic cell extract comprising a first cation exchange chromatography step and a second anion exchange chromatography step.
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公开(公告)号：ES2541358T3
公开(公告)日：2015-07-17
申请号：ES03756575
申请日：2003-10-15
Applicant: UCB PHARMA SA
Inventor： HUMPHREYS DAVID , CHAPMAN ANDREW , ROBINSON MARTYN , SPITALI MARIANGELA
IPC: C12N1/20 , C07K14/245 , C12N1/21 , C12N15/31
Abstract: Una célula hospedadora E. coli que expresa un anticuerpo recombinante caracterizada por que la célula hospedadora E. coli ha sido modificada genéticamente para cambiar el punto isoeléctrico de la proteína PstS de fijación de fosfato de E. coli y en donde el punto isoeléctrico se ha alterado mediante: la adición de una etiqueta de poliácido aspártico al terminal C de la proteína de fijación al fosfato y/o el cambio de uno o más restos de aminoácido situados en la superficie de la proteína PstS de fijación de fosfato de E. coli por: (a) sustitución de uno o más restos de lisina y/o arginina por ácido aspártico o ácido glutámico o (a) sustitución de uno o más restos de ácido aspártico y/o ácido glutámico por lisina o arginina.
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公开(公告)号：CA2584226A1
公开(公告)日：2006-05-26
申请号：CA2584226
申请日：2005-11-16
Applicant: UCB PHARMA SA
Inventor： SPITALI MARIANGELA , SEHDEV MUKESH
Abstract: The present invention relates to the manufacture of recombinant antibodies o f therapeutic quality. In particular, the invention relates to methods for increasing the yield of functional antibody from large scale fermentations whereby a cultured host cell sample is subjected to a non-lysing treatment.
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公开(公告)号：AU2005305681A1
公开(公告)日：2006-05-26
申请号：AU2005305681
申请日：2005-11-16
Applicant: UCB PHARMA SA
Inventor： SPITALI MARIANGELA , SEHDEV MUKESH
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Patent Agency Ranking
公开(公告)号：CA2805132C
公开(公告)日：2020-01-14
申请号：CA2805132
申请日：2011-07-26
Applicant: UCB PHARMA SA
Abstract: The present invention relates to a process for the purification of an antibody fragment from a periplasmic cell extract comprising a first cation exchange chromatography step and a second anion exchange chromatography step.
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公开(公告)号：CA2805132A1
公开(公告)日：2012-02-02
申请号：CA2805132
申请日：2011-07-26
Applicant: UCB PHARMA SA
Abstract: The present invention relates to a process for the purification of an antibody fragment from a periplasmic cell extract comprising a first cation exchange chromatography step and a second anion exchange chromatography step.
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公开(公告)号：AU2003301318B2
公开(公告)日：2008-09-04
申请号：AU2003301318
申请日：2003-10-15
Applicant: UCB PHARMA SA
Inventor： CHAPMAN ANDREW PAUL , HUMPHREYS DAVID PAUL , ROBINSON MARTYN KIM , SPITALI MARIANGELA
IPC: C12N1/20 , C07K14/245 , C12N1/21 , C12N15/31
Abstract: The present invention provides an E. coli host cell expressing a recombinant antibody characterized in that the E. coli host cell has been genetically modified in order to change at least one physical property of one or more E. coli proteins which in the wild type co-purify with said recombinant antibody.
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公开(公告)号：AU2005305700A1
公开(公告)日：2006-05-26
申请号：AU2005305700
申请日：2005-11-16
Applicant: UCB PHARMA SA
Inventor： SEHDEV MUKESH , SPITALI MARIANGELA
Abstract: The present invention relates to the manufacture of recombinant antibodies of therapeutic quality. In particular, the invention relates to methods for increasig the yield of cuntional antibody from large scale fermentations whereby a cultured host cell sample is subjected to a freeze-thaw treatment step.
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公开(公告)号：BR112013000623A2
公开(公告)日：2016-05-24
申请号：BR112013000623
申请日：2011-07-26
Applicant: UCB PHARMA SA
Abstract: purificação de proteína. a presente invenção diz respeito a um processo para a purificação de um fragmento de anticorpo a partir de um extrato de célula periplásmica que compreende uma primeira etapa de cromatografia de troca de cátion e uma segunda etapa de cromatografia de troca aniônica.
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