公开(公告)号：EP1499889A4

公开(公告)日：2006-11-08

申请号：EP03736550

申请日：2003-05-07

Applicant: UNIV NORTHWESTERN

Inventor： MIRKIN CHAD ,  CAO YUN-WEI ,  JIN RONGCHAO

IPC: G01N21/65 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553 ,  G01N33/566 ,  G01N33/58

CPC classification number: G01N33/587 ,  C12Q1/6816 ,  G01N33/54373 ,  G01N33/583 ,  C12Q2565/632 ,  C12Q2565/519 ,  C12Q2537/125

Abstract: The invention encompasses reagents comprising particles with at least one Raman dye and a specific binding members bound thereto and methods of using such reagents. The invention also encompases reagents of a specific binding member and two or more different Raman dyes and methods for using such reagents. New types of particle probes having a specific binding member bound thereto are described. These reagents are used in a novel detection strategy that utilizes the catalytic properties of the Au nanoparticles to generate a silver coating that can behave as a surface-enhanced Raman scattering (SERS) promoter for the dye-labeled particles that have been captured by target and an underlying chip in microarray format. The strategy provides the high sensitivity and high selectivity attributes of grey-scale scanometric detection but provides a route to multiplexing and ratioing capabilities since a very large number of probes can be designed based upon the concept of using a Raman tag as a spectroscopic fingerprint in detection. These spectra are used as fingerprints to differentiate oligonucleotide or other targets in one solution. This method has been used to distinguish six dissimilar DNA targets with six Raman labeled nanoparticle probes, and also two RNA targets with single nucleotide polymorphisms (SNPs).

公开(公告)号：WO03095973A2

公开(公告)日：2003-11-20

申请号：PCT/US0314100

申请日：2003-05-07

Applicant: UNIV NORTHWESTERN ,  MIRKIN CHAD ,  CAO YUN-WEI ,  JIN RONGCHAO

Inventor： MIRKIN CHAD ,  CAO YUN-WEI ,  JIN RONGCHAO

IPC: G01N21/65 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553 ,  G01N33/566 ,  G01N33/58 ,  G01N

CPC classification number: G01N33/587 ,  C12Q1/6816 ,  G01N33/54373 ,  G01N33/583 ,  C12Q2565/632 ,  C12Q2565/519 ,  C12Q2537/125

Abstract: The invention encompasses reagents comprising particles with at least one Raman dye and a specific binding members bound thereto and methods of using such reagents. The invention also encompasses reagents of a specific binding member and two or more different Raman dyes and methods for using such reagents. New types of particle probes having a specific binding member bound thereto are described. These reagents are used in a novel detection strategy that utilizes the catalytic properties of the Au nanoparticles to generate a silver coating that can behave as a surface-enhanced Raman scattering (SERS) promoter for the dye-labeled particles that have been captured by target and an underlying chip in microarray format. The strategy provides the high sensitivity and high selectivity attributes of grey-scale scanometric detection but provides a route to multiplexing and ratioing capabilities since a very large number of probes can be designed based upon the concept of using a Raman tag as a spectroscopic fingerprint in detection. These spectra are used as fingerprints to differentiate oligonucleotide or other targets in one solution. This method has been used to distinguish six dissimilar DNA targets with six Raman labeled nanoparticle probes, and also two RNA targets with single nucleotide polymorphisms (SNPs).

Abstract translation: 本发明包括含有至少一种拉曼染料和与其结合的特异性结合成员的颗粒的试剂和使用这些试剂的方法。 本发明还包括特异性结合成员和两种或更多种不同拉曼染料的试剂和使用这些试剂的方法。 描述具有与其结合的特异性结合成员的新型粒子探针。 这些试剂用于一种新的检测策略，其利用Au纳米粒子的催化性能产生可以作为被目标捕获的染料标记的颗粒的表面增强拉曼散射（SERS）启动子的银涂层， 微芯片格式的底层芯片。 该策略提供了灰度扫描测量检测的高灵敏度和高选择性属性，但是提供了多路复用和比较能力的途径，因为可以基于在检测中使用拉曼标签作为光谱指纹的概念来设计非常大量的探针 。 这些光谱用作指纹以在一种溶液中区分寡核苷酸或其他靶标。 该方法已被用于区分六个不同的DNA靶标与六个拉曼标记的纳米粒子探针，以及两个具有单核苷酸多态性（SNPs）的RNA靶标。

公开(公告)号：WO2004105046A2

公开(公告)日：2004-12-02

申请号：PCT/US2004015161

申请日：2004-05-14

Applicant: UNIV ILLINOIS ,  UNIV NORTHWESTERN ,  LIU CHANG ,  ZOU JUN ,  WANG XUEFENG ,  BULLEN DAVID ,  MIRKIN CHAD ,  ZHANG HUA

Inventor： LIU CHANG ,  ZOU JUN ,  WANG XUEFENG ,  BULLEN DAVID ,  MIRKIN CHAD ,  ZHANG HUA

IPC: B81B1/00 ,  B81B3/00 ,  B82B3/00 ,  G01Q60/00 ,  G01Q70/16 ,  G01Q80/00 ,  G03F7/00 ,  G12B21/02 ,  G03F7/20

CPC classification number: G01Q70/16 ,  G01Q70/06 ,  G01Q80/00 ,  G03F7/0002 ,  Y10S977/857 ,  Y10S977/86 ,  Y10S977/875 ,  Y10S977/877

Abstract: A method for fabricating scanning probe microscopy (SPM) probes is disclosed. The probes are fabricated by forming a structural layer on a substrate, wherein the substrate forms a cavity. A sacrificial layer is located between the substrate and the structural layer. Upon forming the structural layer, the sacrificial layer is selectively removed, and the probe is then released from the substrate. The substrate may then later be reused to form additional probes. Additionally, a contact printing method using a scanning probe microscopy probe is also disclosed.

Abstract translation: 公开了一种制造扫描探针显微镜（SPM）探针的方法。 通过在衬底上形成结构层来制造探针，其中衬底形成空腔。 牺牲层位于基底和结构层之间。 在形成结构层时，选择性地去除牺牲层，然后从衬底释放探针。 然后可以再次使用底物以形成另外的探针。 此外，还公开了使用扫描探针显微镜探针的接触印刷法。

公开(公告)号：WO2008091279A3

公开(公告)日：2008-11-13

申请号：PCT/US2007015009

申请日：2007-06-28

Applicant: UNIV NORTHWESTERN ,  MIRKIN CHAD ,  SALAITA KHALID

Inventor： MIRKIN CHAD ,  SALAITA KHALID

IPC: G03F7/00

CPC classification number: G03F7/0002 ,  Y10S977/849 ,  Y10S977/855 ,  Y10S977/856 ,  Y10S977/857 ,  Y10S977/888 ,  Y10T428/24479 ,  Y10T428/24802

Abstract: Lithographic and nanolithographic methods that involve patterning a first compound on a substrate surface, exposing non-patterned areas of the substrate surface to a second compound and removing the first compound while leaving the second compound intact. The resulting hole patterns can be used as templates for either chemical etching of the patterned area of the substrate or metal deposition on the patterned area of the substrate.

Abstract translation: 包括在基底表面上图案化第一化合物的平版印刷和纳米光刻方法，将基底表面的未图案化区域暴露于第二化合物并除去第一化合物同时保留第二化合物。 所得到的孔图案可以用作用于对衬底的图案化区域进行化学蚀刻或在衬底的图案化区域上的金属沉积的模板。

公开(公告)号：WO2004005582A2

公开(公告)日：2004-01-15

申请号：PCT/US0321220

申请日：2003-07-07

Applicant: UNIV NORTHWESTERN ,  MIRKIN CHAD ,  ZHANG YI ,  SALAITA KHALID

Inventor： MIRKIN CHAD ,  ZHANG YI ,  SALAITA KHALID

IPC: B01L3/00 ,  C40B60/14 ,  C25B

CPC classification number: C40B60/14 ,  B01J2219/00387 ,  B01J2219/00436 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01L3/5027 ,  B81C1/00111 ,  B81C1/00714 ,  B82Y10/00 ,  B82Y30/00 ,  B82Y40/00 ,  C25F1/00 ,  C40B40/04 ,  G03F7/0002 ,  H01L51/0006 ,  H01L51/0017

Abstract: The invention provides a simple and convenient strategy for reducing the dimensions of organic micro- and nanostructures on metal surfaces. By varying electrochemical desorption conditions, organic structures patterned by Dip-Pen Nanolithography or any of the micro-contact printing procedures can be gradually desorbed in a controlled fashion. The electrochemical desorption is initiated at the exterior of the feature and moves inward as a function of time. The desorption process and adsorbate desorption are modified and controlled as a function of substrate morphology, adsorbate head and tail groups, and electrolyte solvent and salt. Different nanostructures made of different adsorbates can be miniaturized based upon judicious selection of adsorbate and supporting electrolyte.

Abstract translation: 本发明提供了用于减小金属表面上的有机微米结构和纳米结构的尺寸的简单方便的策略。 通过改变电化学解吸条件，通过蘸笔纳米平版印刷或任何微接触印刷程序图案化的有机结构可以以受控方式逐渐解吸。 电化学解吸在特征外部开始并随时间向内移动。 解吸过程和吸附物解吸作用随着底物形态，吸附头和尾基团以及电解质溶剂和盐而变化和控制。 基于明智选择吸附物和支持电解质，可以使由不同吸附物制成的不同纳米结构小型化。

公开(公告)号：SG11201809614VA

公开(公告)日：2018-11-29

申请号：SG11201809614V

申请日：2017-05-31

Applicant: UNIV NORTHWESTERN

Inventor： MIRKIN CHAD ,  WALKER DAVID ,  HEDRICK JAMES

IPC: B29C67/00

Abstract: Methods and apparatus comprising a dewetting phase and a polymerization liquid that are immiscible, and can be used for the formation of three-dimensional objects, wherein the method does not require a dead zone. Additionally, methods and apparatus that employ an optically transparent cooling apparatus to mitigate heat generated during the fabrication process, and the use of a mobile phase to provide a shearing interface to reduce interfacial adhesive forces.

公开(公告)号：AU2016269532A1

公开(公告)日：2017-01-05

申请号：AU2016269532

申请日：2016-12-08

Applicant: UNIV NORTHWESTERN

Inventor： MIRKIN CHAD ,  GILJOHANN DAVID A ,  SEFEROS DWIGHT ,  PATEL PINAL C

IPC: C12N15/11 ,  C12N15/87

Abstract: Materials and methods for modulating cellular uptake of functionalized nanoparticles are provided. Also provided are materials and methods for modulating the effectiveness of a therapeutic agent with a functionalized nanoparticle.

公开(公告)号：CA2691295A1

公开(公告)日：2009-03-19

申请号：CA2691295

申请日：2008-06-17

Applicant: UNIV NORTHWESTERN

Inventor： MIRKIN CHAD ,  ROZKIEWICZ DOROTA ,  SENESI ANDREW J

IPC: B01J19/00 ,  C40B50/14

Abstract: A method comprises: (i) providing a tip and a substrate surface, (ii) disposing a patterning composition at the end of the tip, (iii) depositing at least some of the patterning composition from the tip to the substrate surface to form a deposit disposed on the substrate surface, wherein the patterning composition comprises at least one patterning species, at least one carrier that is different from the patterning species, and at least one additive different from the patterning species and the carrier.

公开(公告)号：ES2687650T3

公开(公告)日：2018-10-26

申请号：ES09735282

申请日：2009-04-25

Applicant: UNIV NORTHWESTERN

Inventor： MIRKIN CHAD ,  HUO FENGWEI ,  ZHENG ZIJIAN ,  ZHENG GENGFENG

IPC: G03F7/00 ,  B82Y10/00 ,  B82Y40/00

Abstract: Un método para la impresión a escala submicrométrica de signos sobre una superficie de un sustrato, que comprende: revestir una disposición de puntas con una composición para dibujar, comprendiendo la disposición de puntas un polímero elastomérico compresible que comprende una pluralidad de puntas que no están en voladizo que tienen cada una un radio de curvatura menor de aproximadamente 1 μm, y un sustrato común que comprende una capa elastomérica que comprende un polímero elastomérico compresible, estando la disposición de puntas y el sustrato común fijados sobre un soporte rígido y siendo la disposición de puntas, sustrato común y soporte rígido juntos al menos traslúcidos; poner en contacto la superficie del sustrato durante un primer período de tiempo de contacto y una primera presión de contacto con todas o sustancialmente todas las puntas revestidas de la disposición para depositar la composición para dibujar sobre la superficie del sustrato para formar signos sustancialmente uniformes con todas o sustancialmente todas las citadas puntas revestidas, teniendo los signos un tamaño de puntos o anchura de línea de menos de 1 μm.

公开(公告)号：ES2362797T3

公开(公告)日：2011-07-13

申请号：ES06851430

申请日：2006-08-18

Applicant: UNIV NORTHWESTERN

Inventor： MIRKIN CHAD ,  VEGA RAFAEL ,  MASPOCH DANIEL ,  SALAITA KHALID

IPC: G01N33/50

Abstract: Un arreglo que comprende: una superficie de sustrato, en donde la superficie del sustrato incluye sitios de enlazamiento para una partícula biológica y también sitios que no enlazan la partícula biológica, caracterizado porque dichos sitios de enlazamiento incluyen sitios de enlazamiento de iones metálicos, o sitios de enlazamiento para el metal catiónico divalente, en donde los sitios de enlazamiento sobre la superficie de sustrato tienen cada uno una forma y un tamaño; y una partícula biológica dispuesta sobre cada uno de los sitios de enlazamiento, caracterizada porque la partícula biológica es una proteína, un anticuerpo, o una célula.