公开(公告)号：WO1994006020A1

公开(公告)日：1994-03-17

申请号：PCT/JP1993001228

申请日：1993-08-31

Applicant: ALOKA CO., LTD. ,  ABBOTT LABORATORIES ,  TAKEDA, Masaaki ,  KATO, Yuko ,  KATAGI, Hitomi

Inventor： ALOKA CO., LTD. ,  ABBOTT LABORATORIES

IPC: G01N35/06

CPC classification number: G01M3/26 ,  G01N35/1011 ,  G01N2035/1025

Abstract: A leakage detection method in an automatic pipetting apparatus for objectively judging leakage by measuring an internal pressure of a pump connected to a nozzle tip. At Step 202, distilled water (90) is sucked, and when an internal pressure P1 of a pump after completion of suction is near the atmospheric pressure, leakage is identified. At Step 203, the tip (36) is kept at halt for 30 seconds, for example, without allowing it to rise after the internal pressure P1 of the pump after completion of suction is measured at Step 202. The internal pressure P2 of the pump is again measured by a pressure sensor. When this internal pressure P2 is different from the internal pressure P1 previously measured, leakage is identified. Further, at Step 204, sucked distilled water (90) is discharged into the same test tube (62), and the internal pressure of the pump is monitored by the pressure sensor. If any sharp fluctuation of the internal pressure is found, leakage is judged. When leakage is detected, leakage detection measurement is terminated.

Abstract translation: 一种自动移液装置中的泄漏检测方法，用于通过测量与喷嘴头连接的泵的内部压力来客观地判断泄漏。 在步骤202中，抽吸蒸馏水（90），并且当抽吸结束后的泵的内部压力P1接近大气压力时，识别出泄漏。 在步骤203中，例如在步骤202中，在吸附完毕后测量泵的内部压力P1之后，例如，在步骤202中，将尖端（36）保持停止30秒，而不会使其升高。泵的内部压力P2 再次由压力传感器测量。 当该内部压力P2与先前测量的内部压力P1不同时，识别泄漏。 此外，在步骤204中，将吸入的蒸馏水（90）排出到同一试管（62）中，通过压力传感器监测泵的内部压力。 如果发现内部压力的急剧波动，则判断泄漏。 当检测到泄漏时，泄漏检测测量结束。

公开(公告)号：WO1994002067A1

公开(公告)日：1994-02-03

申请号：PCT/US1993006011

申请日：1993-06-23

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  HAYES, James, Patrick ,  TIEFENTHAL, James, Lawrence ,  McCAMISH, Mark, Anthony ,  ROSS, Jeffrey, R.

IPC: A61B05/05

CPC classification number: A61B5/1106 ,  A61B5/4519 ,  A61B5/6826

Abstract: An apparatus (10) is disclosed which may be employed for determining the relaxation rate of a muscle which has been caused to contract in response to an electrical stimulus. The apparatus comprises a means (13) for supporting the forearm and wrist of a person in a particular palm-up position with the wrist extended; a means (47) for measuring the force exerted upon a load cell (43) by the person's thumb during a test cycle, a means (70) for providing an electrical stimulus to the person's ulnar nerve, and a computer (65) for receiving, storing, plotting and analyzing the force data collected by the transducer (43).

Abstract translation: 公开了一种可用于确定响应于电刺激而导致收缩的肌肉松弛率的装置（10）。 该装置包括一个装置（13），用于在手腕延伸的情况下，以特定的掌声位置来支撑人的前臂和手腕; 一种用于在测试循环期间测量由人的拇指施加在测力传感器（43）上的力的装置（47），用于向人的尺神经提供电刺激的装置（70），以及用于接收 ，存储，绘制和分析由换能器（43）收集的力数据。

公开(公告)号：WO1994001776A1

公开(公告)日：1994-01-20

申请号：PCT/US1993006459

申请日：1993-07-08

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  WINKLER, Martin, A. ,  PAN, Alfred, A.

IPC: G01N33/569

CPC classification number: G01N33/56905 ,  C07K14/44 ,  Y10S435/967 ,  Y10S530/816 ,  Y10S530/822

Abstract: An assay to confirm the presence of antibodies to T. cruzi in a test sample. The assay comprises detecting the presence of antibody to three T. cruzi antigens, Gp90, Gp60/50 and LPPG in a test sample. The presence of antibody in the test sample to at least two of three T. cruzi antibodies is indicative of a confirmed reactive sample. Also provided are diagnostic reagents for detection of T. cruzi, a process for purifying GP60/50, a process for linking a protein and LPPG, and diagnostic test kits for use when assaying for antibodies to T. cruzi.

Abstract translation: 用于确认测试样品中克氏锥虫抗体存在的测定。 该测定法包括检测测试样品中三种克氏锥虫抗原，Gp90，Gp60 / 50和LPPG的抗体的存在。 测试样品中的抗体与三种克氏锥虫抗体中的至少两种的存在表明确认的反应性样品。 还提供了用于检测克氏锥虫的诊断试剂，用于纯化GP60 / 50的方法，用于连接蛋白质和LPPG的方法，以及用于当测定克氏锥虫抗体时使用的诊断试剂盒。

公开(公告)号：WO1993025672A1

公开(公告)日：1993-12-23

申请号：PCT/US1993005509

申请日：1993-06-09

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  BAKER, Harold, Nordean ,  ENG, Katherine, Kit-Ming ,  GARNER, William, Darwin ,  MASSEI, Michael, Kyle ,  NECKLAWS, Elizabeth, Christine ,  OSIKOWICZ, Eugene, Walter ,  RAMP, Sally, Kay ,  TRACH, Paula

IPC: C12N09/96

CPC classification number: C07J41/0016 ,  G01N33/743 ,  Y10S435/962 ,  Y10S436/817 ,  Y10S436/825

Abstract: The present invention relates to the measurement of estradiol using competitive immunoassay methods. The inventors unexpectedly discovered that estrone and its derivatives conjugated to a label is a particularly effective tracer when used in conjunction with estradiol specific antibodies to determine estradiol levels in fluid samples. The present invention also utilizes 5 alpha -dihydrotestosterone to enhance the assay performance.

Abstract translation: 本发明涉及使用竞争性免疫测定方法测量雌二醇。 本发明人意外地发现，当与雌二醇特异性抗体结合使用以测定流体样品中的雌二醇水平时，与标记物缀合的雌酮及其衍生物是特别有效的示踪剂。 本发明还利用5α-二氢睾酮来提高测定性能。

公开(公告)号：WO1993024656A1

公开(公告)日：1993-12-09

申请号：PCT/US1993004863

申请日：1993-05-24

Applicant: ABBOTT LABORATORIES ,  MARSHALL, Ronald, L. ,  CARRINO, John, J. ,  SUSTACHEK, Joann, C.

Inventor： ABBOTT LABORATORIES

IPC: C12Q01/68

CPC classification number: C12Q1/6862 ,  Y10S435/81 ,  C12Q2525/186 ,  C12Q2537/157 ,  C12Q2533/101 ,  C12Q2527/137

Abstract: The present invention involves a method of amplifying RNA by producing complementary DNA (cDNA) by reverse transcription of RNA, and amplification of the cDNA sequences. The analysis of the amplified material facilitates the detection of pathogens and disease states associated with the presence of particular nucleic acid sequences, so the present invention is important in medical diagnostic procedures. A method of producing cDNA of predetermined length is also disclosed.

Abstract translation: 本发明涉及通过逆转录RNA产生互补DNA（cDNA）和cDNA序列扩增来扩增RNA的方法。 扩增物质的分析有助于检测与特定核酸序列存在相关的病原体和疾病状态，因此本发明在医学诊断程序中是重要的。 还公开了一种产生预定长度的cDNA的方法。

公开(公告)号：WO1993021529A1

公开(公告)日：1993-10-28

申请号：PCT/US1993002831

申请日：1993-03-26

Applicant: DUKE UNIVERSITY ,  ABBOTT LABORATORIES

Inventor： DUKE UNIVERSITY ,  ABBOTT LABORATORIES ,  MARKS, Jeffrey, R. ,  IGLEHART, J., Dirk ,  DAVIDOFF, Andrew, M. ,  HENSLEE, Jerry, G.

IPC: G01N33/574

CPC classification number: G01N33/57496 ,  G01N33/564 ,  G01N33/57407 ,  Y10S436/813

Abstract: The present invention relates to the finding that the class of mutant p53 proteins which bind to HSP70 defines a group of mutant p53 proteins which elicit serum autoantibodies. Thus, disclosed is a method of classifying tumor cells for the ability to produce serum p53 autoantibodies in a patient carrying such tumor cells, a method of detecting tumor cells containing a mutant p53 protein capable of forming a complex with a 70 kilodalton heat shock protein (hsp70) in the cells in a patient, a method of distinguishing tumor cells capable of causing more aggressive disease in a patient carrying the tumor cells, and a method of monitoring a patient for the recurrence of disease in a patient previously diagnosed as carrying tumor cells.

Abstract translation: 本发明涉及结合HSP70的突变型p53蛋白的类别定义了一组引起血清自身抗体的突变型p53蛋白。 因此，所公开的是分类肿瘤细胞在携带这种肿瘤细胞的患者中产生血清p53自身抗体的能力的方法，检测含有能够与70千道尔顿热休克蛋白形成复合物的突变型p53蛋白的肿瘤细胞的方法（ hsp70），在携带肿瘤细胞的患者中区分能够引起更积极的疾病的肿瘤细胞的方法，以及监测患者在先前诊断为携带肿瘤细胞的患者中的疾病复发的方法 。

公开(公告)号：WO1993021147A1

公开(公告)日：1993-10-28

申请号：PCT/US1993001134

申请日：1993-02-09

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  MILLER, Robert, Harold ,  PARLET, Nickki, Lynn ,  CIPOLLO, Kent, Lee ,  DHAON, Madhup, Krishna ,  HOUBION, John, Andre ,  LUNDELL, Edwin, Orville

IPC: C07C205/00

CPC classification number: A61K31/40 ,  A61K31/195 ,  Y10S514/866 ,  Y10S514/909

Abstract: A method for administering pyruvate is disclosed which comprises administering a therapeutically effective amount of a pyruvate precursor to a mammal in the form of pyruvamide or a pyruvyl-amino acid. The pyruvyl-amino acid is preferably selected from the group comprising pyruvyl-glycine, pyruvyl-alanine, pyruvyl-leucine, pyruvyl-valine, pyruvyl-isoleucine, pyruvyl-phenylalanine, pyruvyl-proline and pyruvyl-sarcosine, and their amides and esters as well as their salts. Associated with the administration of a pyruvate precursor to a mammal in accordance with this invention are improved insulin resistance, lower fasting insulin levels, and reduced fat gain. Novel methods of synthesizing several pyruvate precursors are also disclosed.

公开(公告)号：WO1993020450A1

公开(公告)日：1993-10-14

申请号：PCT/US1993002811

申请日：1993-03-24

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  CLARK, Frederic, L. ,  CLIFT, Gilbert ,  HENDRICK, Kendall, B. ,  LAGOCKI, Peter, A. ,  MARTIN, Richard, R. ,  MITCHELL, James, E. ,  MOORE, Larry, W. ,  PENNINGTON, Charles, D. ,  WALKER, Edna, S. ,  SMITH, Jane, B. ,  TAYI, Apparao ,  VAUGHT, James, A. ,  YOST, David, A. ,  KANEWSKE, William, J., III

IPC: G01N35/02

CPC classification number: H01J49/04 ,  B01L3/08 ,  B01L3/5025 ,  B01L3/5085 ,  B01L3/50853 ,  B01L9/06 ,  B01L2200/142 ,  B01L2300/042 ,  B01L2300/043 ,  B29C45/00 ,  B29K2995/0032 ,  B29L2023/22 ,  G01N21/03 ,  G01N21/253 ,  G01N33/5302 ,  G01N33/537 ,  G01N33/538 ,  G01N33/542 ,  G01N33/54313 ,  G01N35/0092 ,  G01N35/0099 ,  G01N35/025 ,  G01N35/1065 ,  G01N2001/007 ,  G01N2035/00168 ,  G01N2035/00287 ,  G01N2035/00356 ,  G01N2035/00386 ,  G01N2035/00455 ,  G01N2035/00475 ,  G01N2035/00495 ,  G01N2035/00524 ,  G01N2035/00752 ,  G01N2035/0093 ,  G01N2035/0405 ,  G01N2035/0436 ,  G01N2035/0441 ,  G01N2035/0443 ,  G01N2035/0444 ,  G01N2035/0448 ,  G01N2035/0453 ,  G01N2035/0465 ,  G01N2035/1025 ,  G01N2035/1076 ,  G01N2035/1086 ,  Y10S435/809 ,  Y10S436/807 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/119163 ,  Y10T436/2575

Abstract: An automated, continuous and random access analytical system (18), having apparatus and methodology capable of simultaneously performing multiple assays of liquid samples using different assay methodologies, and providing continuous and random access while performing a plurality of different assays on the same or different samples during the same time period, is disclosed. A method is also disclosed of operating an automated continuous and random access analytical system (18) capable of simultaneously effecting multiple assays of a plurality of liquid samples wherein scheduling of various assays of the plurality of liquid samples is followed by creating a unit dose disposable and separately transferring a first liquid sample (26) and reagents (30) to a reaction vessel (34) without initiation of an assay reaction sequence, followed by physical transfer of the unit dose disposable to a processing workstation (52), whereby a mixture of the unit dose disposable reagents and sample (34) are achieved during incubation. The system (18) is capable of performing more than one scheduled assay in any order, and assays where more than such scheduled assays are presented. The automated, continuous and random access analytical system (18) is also capable of analyzing the incubated reaction mixtures independently and individually by at least two assay procedures.

Abstract translation: 一种自动化，连续和随机存取分析系统（18），其具有能够使用不同测定方法同时进行液体样品的多个测定的装置和方法，以及在相同或不同样品上进行多个不同测定时提供连续和随机存取 在同一时期内披露。 还公开了一种操作自动连续和随机存取分析系统（18）的方法，其能够同时实现多个液体样品的多次测定，其中多个液体样品的各种测定的调度之后是产生单位剂量一次性和 将第一液体样品（26）和试剂（30）分别转移到反应容器（34）而不引发测定反应顺序，然后将单位剂量的一次性物理转移到处理工作站（52），由此将 在孵育期间实现单位剂量一次性试剂和样品（34）。 系统（18）能够以任何顺序执行多于一个的计划分析，以及多于此类调度测定的测定。 自动化，连续和随机存取分析系统（18）还能够通过至少两种测定程序独立地和单独地分析温育的反应混合物。

公开(公告)号：WO1993020074A1

公开(公告)日：1993-10-14

申请号：PCT/US1993002832

申请日：1993-03-26

Applicant: ABBOTT LABORATORIES ,  MATTINGLY, Phillip, G.

Inventor： ABBOTT LABORATORIES

IPC: C07D455/04

CPC classification number: C07D405/12 ,  C07D219/02 ,  C07D219/04 ,  C07H21/00

Abstract: Novel haptens and related conjugates based on acridine-9-carboxylic acid, as well as methods for making and using such conjugates. Haptens based on the above core structure may be substituted at positions 3 or 6. Using tethered intermediates, immunogens, tracers, solid supports and labeled oligonucleotides are all described; as are methods for using the intermediates to prepare the conjugates, methods of using the conjugates to make and purify antibodies, as assay tracers, and in nucleic acid hybridization assays. Kits containing haptenated oligonucleotides and anti-hapten conjugates are also described.

Abstract translation: 基于吖啶-9-羧酸的新型半抗原和相关缀合物，以及制备和使用这些缀合物的方法。 基于上述核心结构的半抗原可以在3或6位置取代。使用系链中间体，免疫原，示踪物，固体支持物和标记的寡核苷酸都被描述; 以及使用中间体制备缀合物的方法，使用缀合物制备和纯化抗体的方法，作为测定示踪剂以及核酸杂交测定法。 还描述了含有半抗体的寡核苷酸和抗半抗原缀合物的试剂盒。

公开(公告)号：WO1993018409A1

公开(公告)日：1993-09-16

申请号：PCT/JP1992001681

申请日：1992-12-22

Applicant: ALOKA CO., LTD. ,  ABBOTT LABORATORIES ,  KAWANABE, Junichi ,  TAKEDA, Masaaki ,  KATAGI, Hitomi ,  KATO, Yuko ,  PELLETIER, Brent, Allen

Inventor： ALOKA CO., LTD. ,  ABBOTT LABORATORIES

IPC: G01N35/06

CPC classification number: G01N35/1016 ,  G01F23/14 ,  G01N2035/1018

Abstract: Purpose: to judge shortage of a discharge quantity, by identifying the presence of any bubbles at the time of discharge. Constitution: characteristics (101) involving bubbles provides a different internal pressure of a nozzle at nearly the end of discharge of a sample than that provided by characteristics (100) not involving bubbles. The nozzle internal pressure is detected at the time of judgement (105), and this detection value is compared with a threshold value. Accordingly, the occurrence of the bubbles can be judged, and shortage of a discharge quantity is judged on the basis of this judgement to give an alarm.

Abstract translation: 目的：通过确定放电时气泡的存在来判断排放量的不足。 结构：涉及气泡的特性（101）提供了比不涉及气泡的特性（100）提供的样品几乎在样品放电结束时喷嘴的内部压力不同。 在判断时检测喷嘴内压（​​105），将该检测值与阈值进行比较。 因此，可以判断气泡的发生，并且基于该判断来判断排出量的不足以给出报警。