公开(公告)号：US08770863B2

公开(公告)日：2014-07-08

申请号：US13487734

申请日：2012-06-04

Applicant: Terry L. Cooke ,  Christopher S. Houser ,  Ronald A. Leonard ,  James M. Wilson

Inventor： Terry L. Cooke ,  Christopher S. Houser ,  Ronald A. Leonard ,  James M. Wilson

IPC: G02B6/36 ,  G02B6/38

CPC classification number: G02B6/3831 ,  G02B6/3851 ,  G02B6/3885 ,  G02B6/3895 ,  G02B6/3897

Abstract: A multi-fiber fiber optic connector is configured to include a switchable polarity key that can be used to define first and second polarity configurations for the connector. The connector has a multi-fiber ferrule surrounded by an inner housing. The inner housing has top and bottom recesses sized to accommodate the polarity key. The polarity key is removably secured in either a top or bottom recess using a latching feature. The polarity of the connector can be switched by moving the polarity key from one position in the connector to the other rather than having to disassemble the connector.

Abstract translation: 多光纤光纤连接器被配置为包括可切换的极性键，其可以用于限定连接器的第一和第二极性配置。 连接器具有被内壳包围的多纤维套圈。 内壳具有尺寸适应极性键的顶部和底部凹槽。 使用锁定特征，极性键可拆卸地固定在顶部或底部凹部中。 可以通过将极性键从连接器中的一个位置移动到另一个而不必拆卸连接器来切换连接器的极性。

公开(公告)号：US20130323226A1

公开(公告)日：2013-12-05

申请号：US13985630

申请日：2012-02-17

Applicant: James M. Wilson ,  Christie L. Bell ,  Luc H. Vandenberghe

Inventor： James M. Wilson ,  Christie L. Bell ,  Luc H. Vandenberghe

IPC: A61K31/7088 ,  A61K31/685 ,  A61K38/47

CPC classification number: A61K31/7088 ,  A61K31/685 ,  A61K38/47 ,  C12N15/86 ,  C12N2750/14043 ,  C12N2750/14045 ,  C12N2810/6027

Abstract: A method of altering the targeting and/or cellular uptake efficiency of an adeno-associated virus (AAV) viral vector having a capsid containing an AAV9 cell surface binding domain is described. The method involves modifying a clade F cell surface receptor which comprises a glycan having a terminal sialic acid residue and a penultimate β-galactose residue. The modification may involve retargeting the vector by temporarily functionally ablate AAV9 binding in a subset of cells, thereby redirecting the vector to another subset of cells. Alternatively, the modification may involve increasing cellular update efficiency by treating the cells with a neuraminidase to expose cell surface β-galactose. Also provided are compositions containing the AAV9 vector and a neuraminidase. Also provided is a method for purifying AAV9 using β-galactose linked to solid support. Also provided are mutant vectors which have been modified to alter their targeting specificity, including mutant AAV9 in which the galactose binding domain is mutated and AAV in which an AAV9 galactose binding domain is engineered.

Abstract translation: 描述了改变具有包含AAV9细胞表面结合结构域的衣壳的腺相关病毒（AAV）病毒载体的靶向和/或细胞摄取效率的方法。 该方法包括修饰进化枝F细胞表面受体，其包含具有末端唾液酸残基的聚糖和倒数第二半乳糖残基。 该修饰可能涉及通过临时功能地消融细胞子集中的AAV9结合来重新定向载体，从而将载体重定向到另一个细胞亚群。 或者，修饰可以涉及通过用神经氨酸酶处理细胞以暴露细胞表面β-半乳糖来增加细胞更新效率。 还提供了含有AAV9载体和神经氨酸酶的组合物。 还提供了使用与固体支持物连接的β-半乳糖来纯化AAV9的方法。 还提供已被修饰以改变其靶向特异性的突变载体，包括其中半乳糖结合结构域突变的突变AAV9和其中AAV9半乳糖结合结构域被工程化的AAV。

公开(公告)号：US20130322825A1

公开(公告)日：2013-12-05

申请号：US13487734

申请日：2012-06-04

Applicant: Terry L. Cooke ,  Christopher S. Houser ,  Ronald A. Leonard ,  James M. Wilson

Inventor： Terry L. Cooke ,  Christopher S. Houser ,  Ronald A. Leonard ,  James M. Wilson

IPC: G02B6/38

CPC classification number: G02B6/3831 ,  G02B6/3851 ,  G02B6/3885 ,  G02B6/3895 ,  G02B6/3897

Abstract: A multi-fiber fiber optic connector is configured to include a switchable polarity key that can be used to define first and second polarity configurations for the connector. The connector has a multi-fiber ferrule surrounded by an inner housing. The inner housing has top and bottom recesses sized to accommodate the polarity key. The polarity key is removably secured in either a top or bottom recess using a latching feature. The polarity of the connector can be switched by moving the polarity key from one position in the connector to the other rather than having to disassemble the connector.

Abstract translation: 多光纤光纤连接器被配置为包括可切换的极性键，其可以用于限定连接器的第一和第二极性配置。 连接器具有被内壳包围的多纤维套圈。 内壳具有尺寸适应极性键的顶部和底部凹槽。 使用锁定特征，极性键可拆卸地固定在顶部或底部凹部中。 可以通过将极性键从连接器中的一个位置移动到另一个而不必拆卸连接器来切换连接器的极性。

公开(公告)号：US08394386B2

公开(公告)日：2013-03-12

申请号：US11587543

申请日：2005-04-27

Applicant: James M. Wilson

Inventor： James M. Wilson

IPC: A61K39/23 ,  A61K39/116 ,  C12Q1/70

CPC classification number: C12N15/86 ,  A61K2039/5256 ,  A61K2039/545 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2750/14143 ,  C12N2760/14122 ,  C12N2760/14134 ,  C12N2830/002 ,  C12N2830/55 ,  Y02A50/41 ,  Y02A50/412

Abstract: An immunogenic regimen is provided. The regimen involves sequential administration of a recombinant adenoviral vector and a recombinant adeno-associated viral vector, each of which delivers a heterologous expression cassette encoding the same immunogenic product, or a cross-reactive immunogenic product. Also provided are products containing the vectors for use in the regimen of the invention.

Abstract translation: 提供免疫原性方案。 该方案包括顺序施用重组腺病毒载体和重组腺相关病毒载体，每个载体递送编码相同免疫原性产物的异源表达盒或交叉反应性免疫原性产物。 还提供含有用于本发明方案的载体的产品。

公开(公告)号：US20120093853A1

公开(公告)日：2012-04-19

申请号：US13084596

申请日：2011-04-12

Applicant: James M. Wilson ,  Guangping Gao ,  Soumitra Roy

Inventor： James M. Wilson ,  Guangping Gao ,  Soumitra Roy

IPC: A61K31/7088 ,  A61K39/00 ,  A61P37/04 ,  A61P31/18 ,  A61P31/14 ,  A61P31/16 ,  A61P31/22 ,  A61P31/20 ,  A61P31/04 ,  A61P31/10 ,  A61P33/02 ,  A61P33/10 ,  A61P33/12 ,  A61P35/00 ,  C12N15/861

CPC classification number: C12N15/86 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10362 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2750/14122 ,  C12N2760/20122 ,  C12N2760/20134 ,  C12N2830/001 ,  C12N2830/55

Abstract: A recombinant vector comprises a simian adenovirus capsid and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.

Abstract translation: 重组载体包含猿猴腺病毒衣壳和在调节序列控制下的异源基因。 还公开了表达猿腺病毒基因的细胞系。 提供了使用载体和细胞系的方法。

公开(公告)号：US20120093778A1

公开(公告)日：2012-04-19

申请号：US13337608

申请日：2011-12-27

Applicant: James M. Wilson ,  Guangping Gao ,  Soumitra Roy

Inventor： James M. Wilson ,  Guangping Gao ,  Soumitra Roy

IPC: A61K35/76 ,  C12N5/10 ,  C12N15/861 ,  C07K14/075 ,  C12N7/01 ,  C12N15/34

CPC classification number: A61K39/235 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/525 ,  A61K2039/5256 ,  A61K2039/57 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10022 ,  C12N2710/10321 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10362 ,  C12N2710/20022 ,  C12N2740/16122 ,  C12N2740/17022 ,  C12N2750/14122 ,  C12N2760/14134 ,  C12N2760/16134 ,  C12N2760/20122 ,  C12N2830/002 ,  C12N2830/55

Abstract: A recombinant vector comprises simian adenovirus sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.

Abstract translation: 重组载体包含猿猴腺病毒序列和在调节序列控制下的异源基因。 还公开了表达猿腺病毒基因的细胞系。 提供了使用载体和细胞系的方法。

公开(公告)号：US08076257B1

公开(公告)日：2011-12-13

申请号：US12386736

申请日：2009-04-22

Applicant: James M. Wilson

Inventor： James M. Wilson

IPC: C04B35/465 ,  C04B35/468 ,  H01G4/06

CPC classification number: H01G4/30 ,  C04B35/462 ,  C04B35/47 ,  C04B35/6264 ,  C04B35/62685 ,  C04B2235/3201 ,  C04B2235/3213 ,  C04B2235/3215 ,  C04B2235/3224 ,  C04B2235/3227 ,  C04B2235/3229 ,  C04B2235/3232 ,  C04B2235/3234 ,  C04B2235/3239 ,  C04B2235/3251 ,  C04B2235/3258 ,  C04B2235/3262 ,  C04B2235/3275 ,  C04B2235/3279 ,  C04B2235/3284 ,  C04B2235/3287 ,  C04B2235/3298 ,  C04B2235/3409 ,  C04B2235/3418 ,  C04B2235/6025 ,  C04B2235/77 ,  C04B2235/9623 ,  H01G4/1227 ,  H01G4/129

Abstract: A bismuth sodium titanate (Bi0.5Na0.5TiO3) base material is modified by the partial substitution of aliovalent A-site cations such as barium (as BaO) or strontium (as SrO), as well as certain b-site donor/acceptor dopants and sintering aids to form a multi-phase system, much like known “core/shell” X7R dielectrics based solely on BaTiO3. The resulting ceramic dielectric composition is particularly suitable for producing a multilayer ceramic capacitor (10) that maintains high dielectric constant (and thus the capability of maintaining high capacitance) over a broad temperature range of from about 150° C. to about 300° C. Such capacitors (10) are appropriate for high temperature power electronics applications in fields such as down-hole oil and gas well drilling.

Abstract translation: 钛酸铋（Bi0.5Na0.5TiO3）基材料通过部分取代别名的A位阳离子如钡（如BaO）或锶（作为SrO）以及某些b位供体/受体掺杂剂 和烧结助剂形成多相系统，这与仅仅基于BaTiO3的已知的“核/壳”X7R电介质相似。 所得到的陶瓷电介质组合物特别适用于制备在大约150℃至大约300℃的宽温度范围内保持高介电常数（并因此保持高电容的能力）的多层陶瓷电容器（10）。 这种电容器（10）适用于诸如井下油气井钻井等领域的高温电力电子应用。

公开(公告)号：US20100260799A1

公开(公告)日：2010-10-14

申请号：US12744405

申请日：2008-11-24

Applicant: Soumitra Roy ,  James M. Wilson ,  Luc H. Vandenberghe

Inventor： Soumitra Roy ,  James M. Wilson ,  Luc H. Vandenberghe

IPC: A61K39/235 ,  C12N7/01 ,  C07H21/04 ,  C12N15/63 ,  C07K14/01 ,  C12N9/50 ,  A61P31/12

CPC classification number: C12N7/00 ,  C12N2710/10321 ,  C12N2710/10322 ,  C12N2710/10343

Abstract: A recombinant vector comprises simian adenovirus SAdV-31 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus SAdV-31 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.

Abstract translation: 重组载体包含猿猴腺病毒SAdV-31序列和在调控序列控制下的异源基因。 也公开了表达猿猴腺病毒SAdV-31基因的细胞系。 提供了使用载体和细胞系的方法。

公开(公告)号：US07725565B2

公开(公告)日：2010-05-25

申请号：US12274243

申请日：2008-11-19

Applicant: Peter Chao-yuan Li ,  Jeffrey R. Collmann ,  Jane W. Blake ,  Mark G. Polyak ,  James M. Wilson ,  Jae In Yoon

Inventor： Peter Chao-yuan Li ,  Jeffrey R. Collmann ,  Jane W. Blake ,  Mark G. Polyak ,  James M. Wilson ,  Jae In Yoon

IPC: G06F15/16 ,  G06F12/00

CPC classification number: G06F17/30867 ,  G06F17/30887 ,  G06F19/00 ,  G06Q10/067 ,  G16H50/80 ,  H04L29/08072 ,  Y02A90/24 ,  Y02A90/26

Abstract: A system and method involves detecting operational social disruptive events on a global scale, assigning disease event staging and warnings to express data in more simplistic terms, modeling data in conjunction with linguistics analysis to establish responsive actions, generating visualization and modeling capabilities for communicating information, and modeling disease propagation for containment and forecasting purposes.

Abstract translation: 一种系统和方法涉及在全球范围内检测运营中的社会破坏性事件，分配疾病事件分期和警告以更简单的术语表达数据，结合语言学分析建立数据以建立响应动作，生成用于传达信息的可视化和建模能力， 并为遏制和预测目的建模疾病传播。

公开(公告)号：US20090317417A1

公开(公告)日：2009-12-24

申请号：US12226536

申请日：2007-04-27

Applicant: Luc H. Vandenberghe ,  James M. Wilson

Inventor： Luc H. Vandenberghe ,  James M. Wilson

IPC: A61K39/235 ,  C12N7/00 ,  A61P31/12

CPC classification number: C12N15/86 ,  A61K2039/57 ,  C12N2750/14142 ,  C12N2750/14143

Abstract: A method of reducing the cellular immune response and/or toxicity of AAV-mediated delivery is described. The method provides for masking or ablating a RxxR motif which induces T-cells, and which is located on select AAV capsids. The method further provides for reducing or eliminating heparin binding to an AAV. Also provided are compositions containing modified AAV capsids and methods of using same.

Abstract translation: 描述了减少AAV介导的递送的细胞免疫应答和/或毒性的方法。 该方法提供掩蔽或消融诱导T细胞的RxxR基序，其位于选定的AAV衣壳上。 该方法进一步提供了减少或消除肝素与AAV的结合。 还提供了含有修饰的AAV衣壳的组合物及其使用方法。