公开(公告)号：WO1994013692A1

公开(公告)日：1994-06-23

申请号：PCT/US1993011781

申请日：1993-12-06

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA ,  THE UNITED STATES OF AMERICA, as reprensented by ...

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  THE UNITED STATES OF AMERICA, as reprensented by ... ,  FURCHT, Leo, T. ,  MCCARTHY, James, B. ,  WAHL, Sharon, M. ,  ALLEN, Janice, B.

IPC: C07K05/00

CPC classification number: C07K14/78 ,  A61K38/00

Abstract: A method for treating acute or chronic inflammatory or autoimmune disorders using polypeptides with fibronectin or related activity is provided. The method involves administering an effective amount of one or more polypeptides corresponding to isolated amino acid residue sequences of the 33 kD carboxy terminal heparin-binding region located on the A chain of fibronectin or an RGD-containing amino acid sequence within the 11.5 kD RGDS-mediated cell adhesion region located on all isoforms of fibronectin to effectively suppress inflammation and the accompanying impairment of tissue function in a patient.

Abstract translation: 提供了使用具有纤连蛋白或相关活性的多肽治疗急性或慢性炎性或自身免疫性疾病的方法。 该方法包括施用有效量的一种或多种对应于位于纤连蛋白A链上的33kD羧基末端肝素结合区域的分离的氨基酸残基序列或11.5kD RGDS- 介导的细胞粘附区域位于纤维连接蛋白的所有同种型上，以有效抑制患者的炎症和伴随的组织功能损伤。

公开(公告)号：WO1992015711A1

公开(公告)日：1992-09-17

申请号：PCT/US1992001675

申请日：1992-03-04

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  SANTAMARIA, Pedro ,  BOYCE-JACINO, Michael, Thomas ,  BARBOSA, Jose, Joaquim ,  RICH, Stephen, Saul ,  FARAS, Anthony, James

IPC: C12Q01/68

CPC classification number: C12Q1/6881 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q2600/156 ,  C12Q2525/15

Abstract: The present invention provides a process for determining genotypes in highly polymorphic systems by polymerase chain reaction amplification of cDNA or genomic DNA and direct sequencing polymerase chain reaction products using oligonucleotide primers. More specifically, Class II and Class I HLA genotypes can be unambiguously determined in any subject in 16-24 hours by direct sequencing of DRB, DQB, DQA, DPB, DPA, HLA-A, HLA-B and HLA-C- transcripts enzymatically amplified using a limited number of non-allele-specific oligonucleotides. Total cellular RNA from peripheral blood mononuclear cells is reverse transcribed using antisense primers, specific for different locus (DQB, DQA, DPA, or DPB) or group of loci (DRB1-5, or HLA-A and HLA-B and HLA-C). The synthesized cDNA molecules are then enzymatically amplified using different combinations of oligonucleotides for each locus and directly sequenced with Taq polymerase using an internal oligonucleotide. The sequenced genes are then analyzed.

Abstract translation: 本发明提供了通过使用寡核苷酸引物的cDNA或基因组DNA的聚合酶链反应扩增和直接测序聚合酶链反应产物来确定高度多态性系统中的基因型的方法。 更具体地，可以在任何受试者16-24小时内通过直接测序DRB，DQB，DQA，DPB，DPA，HLA-A，HLA-B和HLA-C转录物来酶解II类和I类HLA基因型 使用有限数量的非等位基因特异性寡核苷酸扩增。 来自外周血单核细胞的总细胞RNA使用对不同基因座（DQB，DQA，DPA或DPB）或基因座组（DRB1-5或HLA-A和HLA-B和HLA-C）的特异性的反义引物进行逆转录 ）。 然后使用每个位点的不同寡核苷酸组合对合成的cDNA分子进行酶扩增，并使用内部寡核苷酸用Taq聚合酶直接测序。 然后分析测序的基因。

公开(公告)号：WO1992012727A1

公开(公告)日：1992-08-06

申请号：PCT/US1992000473

申请日：1992-01-21

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  SKUBITZ, Amy, P., N. ,  FURCHT, Leo, T.

IPC: A61K37/02

CPC classification number: C12N5/0068 ,  A61L27/34 ,  C07K14/78 ,  C12N2533/52 ,  Y10S977/738 ,  Y10S977/813 ,  Y10S977/895 ,  Y10S977/902 ,  Y10S977/915 ,  Y10S977/923

Abstract: Polypeptides derived from domain VI of the amino terminal globule of the A chain of laminin and having sequences of at least about 5 amino acids, and which exhibit cell adhesion and cell spreading capacity are described. Medical devices such as prosthetic implants, per-cutaneous devices and cell culture substrates coated with a composition including the described polypeptides are also provided.

Abstract translation: 描述了衍生自层粘连蛋白A链的氨基末端小球的结构域VI并且具有至少约5个氨基酸的序列并且表现出细胞粘附和细胞扩散能力的多肽。 还提供医疗装置，例如用包含所述多肽的组合物涂覆的假体植入物，皮肤装置和细胞培养基质。

公开(公告)号：WO1992007615A1

公开(公告)日：1992-05-14

申请号：PCT/US1991007952

申请日：1991-10-29

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  SHATFORD, Russell, A. ,  CERRA, Frank, B. ,  NYBERG, Scott, L. ,  HU, Wei-Shou

IPC: A61M37/00

CPC classification number: C12M25/12 ,  A61F2/022 ,  A61K2035/126 ,  A61M1/1625 ,  A61M1/3475 ,  A61M1/3489 ,  C12M25/14 ,  C12N2533/30 ,  C12N2533/54

Abstract: A bioartificial liver (30) to provide liver function to patients with liver insufficiency or failure is disclosed. The bioartificial liver is based on a bioreactor of the type having two fluid paths (42, 46) separated by a permeable medium (38). The bioreactor can be of either hollow fiber or flat-bed configuration. In the configuration using hollow fibers (40), the two fluid paths correspond to the cavity (36) surrounding the hollow fibers (the extracapillary space), and to the lumens of the hollow fibers themselves. Both fluid paths have inlet and outlet ports. Communication between the two fluid paths is across the permeable medium - the hollow fiber material. Hepatocytes (34) are inoculated into the hollow fibers in a solution which quickly forms a highly porous gel. The gel subsequently contracts, leaving an open channel within the hollow fiber adjacent to the gel core entrapped hepatocytes. This channel can be perfused with nutrient media for the hepatocytes.

Abstract translation: 公开了向肝功能不全或失败的患者提供肝功能的生物人造肝（30）。 生物人造肝基于具有由可渗透介质（38）分离的两个流体路径（42,46）的类型的生物反应器。 生物反应器可以是中空纤维或平床构型。 在使用中空纤维（40）的构造中，两个流体路径对应于围绕中空纤维（毛细管外空间）的空腔（36）和中空纤维本身的内腔。 两个流体路径都具有入口和出口。 两条流体路径之间的通信是穿过可渗透介质 - 中空纤维材料。 将肝细胞（34）以快速形成高度多孔凝胶的溶液接种到中空纤维中。 凝胶随后收缩，在中空纤维中留下与凝胶芯捕获的肝细胞相邻的开放通道。 该通道可以用肝细胞的营养培养基灌注。

公开(公告)号：WO1991009054A1

公开(公告)日：1991-06-27

申请号：PCT/US1990007164

申请日：1990-12-06

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  TSILIBARY, Photini-Effie, C.

IPC: C07K07/08

CPC classification number: C07K14/78

Abstract: A polypeptide having the following formula is provided: gly-glu-phe-tyr-phe-asp-leu-arg-leu-lys-gly-asp-lys which can bind heparin and promote cellular adhesion. Medical devices such as prosthetic implants, percutaneous devices and cell culture substrates coated with a composition including the polypeptide are also provided.

Abstract translation: 提供具有下式的多肽：gly-glu-phe-tyr-phe-asp-leu-arg-leu-lys-gly-asp-lys，其可以结合肝素并促进细胞粘附。 还提供了医疗装置，例如假体植入物，经皮装置和用包含多肽的组合物包被的细胞培养基质。

公开(公告)号：WO1991005534A1

公开(公告)日：1991-05-02

申请号：PCT/US1990004476

申请日：1990-08-09

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  BUDD, Jeffrey

IPC: A61H31/02

CPC classification number: A61H9/0078 ,  A61B5/087 ,  A61H2205/08 ,  A61H2230/40 ,  A61M16/0006 ,  A61M2016/0042

Abstract: Persons having respiratory diseases often have treatment programs including a pummeling of the thoracic cavity for the purpose of aiding respiration. A vest (16) as a part of an alternating compression machine (104) is used to eliminate the need for trained physical therapists and rather provide for mechanical thumping. A fitting routine (112) for the vest (16) is provided. A routine (114) for identifying optimal operating frequencies for the machine (104) is also provided, along with an algorithm (156) for selecting appropriate frequencies.

公开(公告)号：WO1991003323A1

公开(公告)日：1991-03-21

申请号：PCT/US1990004867

申请日：1990-08-27

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  LAU, Yuk-Chiu ,  PFENDER, Emil

IPC: B05D05/12

CPC classification number: C23C16/408 ,  C23C16/507 ,  H01L39/2445 ,  Y10S505/73 ,  Y10S505/737

Abstract: A method is provided to apply a coating of a superconducting ceramic oxide to a substrate comprising introducing an atomized aqueous solution comprising at least three metal salts into inductively coupled plasma to deposit a mixed metal oxide on the substrate, which is converted into a superconductive ceramic oxide by post-annealing.

公开(公告)号：WO1990015376A2

公开(公告)日：1990-12-13

申请号：PCT/US1990003209

申请日：1990-06-06

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  ERDMAN, Arthur, G. ,  REKOW, Elizabeth, D. ,  RILEY, Donald, R. ,  KLAMECKI, Barney, E. ,  ZHU, Yang ,  AHN, Jeong-Ho

IPC: G05B19/42

CPC classification number: A61C13/0004 ,  A61C9/0046 ,  A61C9/0093 ,  G05B19/4207

Abstract: The present invention provides a method and apparatus for the automated reproduction of three dimensional objects of complex and unique geometry. A computer acquires data describing an object and its surroundings, constructs a computer-based three dimensional model of the object from that data, superimposes an ideal geometry on the computer-based model, alters the ideal geometry to fit the form and function required of the reproduction, and then guides a milling machine in the fabrication of the reproduction. In contrast to the prior art, the present invention does not require human intervention in the reproduction process. The present invention provides two preferred embodiments for the automated high precision reproduction of objects of complex and unique geometry. Both embodiments can machine reproductions solely from generic forms stored in the computer, thus eliminating the need for scanning. Alternatively, the reproductions may be machined solely from scanned data, without resort to generic forms stored in the computer.

公开(公告)号：WO1990010601A1

公开(公告)日：1990-09-20

申请号：PCT/US1990001123

申请日：1990-03-01

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  HOOPER, Alan

IPC: C02F03/34

CPC classification number: C02F3/1231 ,  C02F3/34 ,  Y02W10/15

Abstract: A method is disclosed for degradation of a halogenated hydrocarbon compound such as trichloroethylene (TCE) which utilizes an ammonia-oxidizing bacterium of the genus Nitrosomonas.

公开(公告)号：WO1990009204A1

公开(公告)日：1990-08-23

申请号：PCT/US1990000588

申请日：1990-01-26

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： REGENTS OF THE UNIVERSITY OF MINNESOTA ,  WIGNESS, Bruce, D. ,  DORMAN, Frank, D.

IPC: A61M25/00

CPC classification number: A61M25/0075 ,  A61M2025/0076

Abstract: A vascular access system (20) utilizing a single-lumen catheter (22) having a bidirectional check valve assembly (24).

Abstract translation: 一种使用具有双向止回阀组件（24）的单腔导管（22）的血管通路系统（20）。