Abstract:
The present invention is directed to methods of manufacture of microfluidic chip such as a plastic microfluidic chips, which has channels packed with polymer-embedded particles and uses thereof. The chip of the present invention is designed for application of an untreated biological sample on the chip thus allowing isolation, purification and detection of biomolecules, such as nucleic acids, proteins or peptides in one step. The invention also provides a microfluidic chip for combined isolation, purification and detection of biomolecules thus providing a complete Lab-on-a-Chip analysis system for biomolecules such as nucleic acids and proteins. The chips of the invention can be adapted to perform highly specific immunoassays and diagnostic test, for example, for diagnosis of infectious agents, such as bacteria, viruses or parasites.
Abstract:
Microfluidic separators for separating multiphase fluids are described. Two or more microfluidic outlet channels within the device meet at an overlap region. The overlap region may be in fluid communication with an inlet channel. The inlet channel and each outlet channel are disposed within different layers of a three-dimensional device. A multiphase fluid flows through an inlet channel into an overlap region from where the separated phases can be withdrawn through the outlet channels.
Abstract:
Die Erfindung betrifft ein Bauelement, umfassend einen Träger aus einem strukturierbaren Material mit mindestens einer durchgehenden Öffnung, die von einer porösen Membran verschlossen ist, dadurch gekennzeichnet, dass die poröse Membran aus der die durchgehende Öffnung umgebenden Fläche des Bauelements herausragt. In manchen Ausgestaltungen umfasst das Bauelement weiterhin ein Trägersubstrat, wobei eine zum Bauelement weisende Seite des Trägersubstrats und die gegenüberliegende Seite des Bauelements vorzugsweise einen Fluidkanal bilden, wobei im bevorzugten Fall die mindestens eine durchgehende Öffnung des Trägers auf ihrer unverschlossenen Seite mit dem Fluidkanal kommuniziert. Das erfindungsgemäße Bauelement eignet sich zum Einbau und zur elektrochemischen Vermessung von Transmembranproteinen, vorzugsweise in Doppel- Lipidschichten. Die Erfindung schlägt auch verschiedene Verfahren zur Herstellung des Bauelements vor.
Abstract:
The present invention relates to a channel structure for a bioreactor, biochemical reactor, chemical reactor or a reformer comprising a plurality of individual layers (1A, 1B, 1C) stacked on one another and having a respective plurality of openings (3) which pass completely through the respective individual layer and which are characterized in that at least two directly adjacent individual layers each have at least one layer section whose openings are arranged in the form of a pattern (4) respectively regular in two dimensions (D1, D2) and in that at least two of the layer sections provided with such a pattern in this manner of directly adjacent individual layers overlap (5) at least in part, wherein the openings of the two at least partly overlapping layer sections are rotated and/or offset with respect to one another in the overlap region.
Abstract:
Methods for molding glass and glass composites, including providing a first structure having a first surface, providing a second structure having a second surface, the second surface being patterned and porous, and disposing between the first and second surfaces an amount of a composition comprising a glass, then heating together the first and second structures and the first amount of the composition sufficiently to soften the first amount of the composition such that the first and second structures, under gravity or an otherwise applied force, move toward each other, such that the pattern of the second surface is formed into the first amount of the composition, then cooling the composition sufficiently to stabilize it, the second structure comprising porous carbon having an open porosity of at least 5% and wherein the amount of the composition is removable from the second surface, without damage to the amount of the composition or to the second surface, such that the second surface is in condition for re-use.
Abstract:
Materials and methods are provided for fabricating microfluidic devices. The materials include low surface energy fluoropolymer compositions having multiple cure functional groups. The materials can include multiple photocurable and/or thermal-curable functional groups such that laminate devices can be fabricated. The materials also substantially do not swell in the presence of hydrocarbon solvents.
Abstract:
Microfluidic devices having wall structures comprised of sintered glass frit and further including a glass, glass-ceramic or ceramic membrane structure sealed by a sintered seal to said wall structures, such that a a fluid passage or chamber is defined at least in part by the wall structures and said membrane structure. This allows for changes in pressure within the fluid passage or chamber to cause deflections of the membrane structure, providing for direct measurement of pressure within the device. The microfluidic device may have both floors and walls of sintered frit, or may have only walls of sintered frit, with planar floor-like substrate structures, thicker than the membrane structure defining the vertical boundaries of the internal passages. The device may include multiple fluid passages or chambers each defined at least in part by a membrane structure. Multiple membrane structures may be used in a single device, and one single membrane structure may be used for multiple passages or chamber.