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    돼지콜레라 바이러스 Ｅｒｎｓ 단백질 및 이에 특이적으로반응하는 단클론항체를 이용한 돼지콜레라 항체 감별진단방법
    102.
    发明公开
    돼지콜레라 바이러스 Ｅｒｎｓ 단백질 및 이에 특이적으로반응하는 단클론항체를 이용한 돼지콜레라 항체 감별진단방법 有权
    SWINE FEVER VIRUS ERNS蛋白质和筛选和检测与其相似的SWINE FEVER病毒抗体的方法

    公开(公告)号：KR1020070116382A

    公开(公告)日：2007-12-10

    申请号：KR1020060050357

    申请日：2006-06-05

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 메디안디노스틱

    Inventor： 송재영 ,  김병한 ,  탁동섭 ,  최은진 ,  임성인 ,  한규하 ,  주후돈 ,  장병식 ,  이현정 ,  박경민

    IPC: C12N15/33 ,  C12N15/09 ,  C12N15/10

    Abstract: A method for screening and detecting a swine fever virus antibody is provided to easily screen and diagnose infected swine fever virus antibody by using a monoclonal antibody specifically reacting with a recombinant swine fever virus Erns protein and secure constant sensitivity regardless of all genotypes of the swine fever virus when using all three kinds of recombinant protein. A recombinant swine fever virus Erns is prepared by expressing a gene encoding three Erns proteins among swine fever viruses having various genotypes using baculovirus. A method for preparing a monoclonal antibody specifically reacting to the Erns comprises the steps of: (a) constructing a swine fever virus LOM strain-derived Erns recombinant gene using a baculovirus expression system; and (b) producing the monoclonal antibody using the recombinant Erns protein. A method for screening and detecting a swine fever virus antibody comprises the steps of: (a) mixing three kinds of Erns recombinant proteins at proper concentration to prepare an antigen cocktail; (b) after diluting the antigen cocktail into a coating buffer solution, pouring it in a plate to be absorbed into the plate; (c) washing the recombinant antigen not-absorbed into the plate to remove it; (d) adding a sample to be tested to the plat to allow the reaction; (e) washing the sample to be tested not specifically bound to the recombinant Erns antigen protein absorbed into the plate to remove it; (f) adding a monoclonal antibody not being bound to the enzyme but being specifically reacted to the Erns protein to allow the reaction; (g) washing the monoclonal antibody not being bound to the recombinant Erns antigen protein absorbed into the plate to remove it; and (h) adding a substrate reacting with the enzyme to determine the swine fever virus infection.

    Abstract translation: 提供了一种筛选和检测猪瘟病毒抗体的方法，通过使用与重组猪瘟病毒Erns蛋白特异性反应的单克隆抗体轻松筛选和诊断感染的猪瘟病毒抗体，无论猪瘟的所有基因型如何 使用全部三种重组蛋白的病毒。 通过使用杆状病毒在具有各种基因型的猪瘟病毒中表达编码三种Erns蛋白质的基因来制备重组猪瘟病毒Erns。 制备与Erns特异性反应的单克隆抗体的方法包括以下步骤：（a）使用杆状病毒表达系统构建猪瘟病毒LOM菌株衍生的Erns重组基因; 和（b）使用重组Erns蛋白产生单克隆抗体。 筛选和检测猪瘟病毒抗体的方法包括以下步骤：（a）以适当浓度混合三种Erns重组蛋白以制备抗原混合物; （b）将抗原混合物稀释到涂布缓冲溶液中后，将其倒入板中吸收到板中; （c）将未吸收的重组抗原洗涤到板中以除去它; （d）将待测试样品加入平板以允许反应; （e）洗涤待测试的样品，未特异性地结合到吸收到板中的重组Erns抗原蛋白质以除去它; （f）加入未结合酶的单克隆抗体，但与Erns蛋白特异性反应以进行反应; （g）洗涤未结合到吸附到板中的重组Erns抗原蛋白的单克隆抗体以除去它; 和（h）加入与酶反应的底物以确定猪瘟病毒感染。

    일본 뇌염 바이러스 유전자 피알엠이을 발현하는 돼지 아데노바이러스 3형
    104.
    发明公开
    일본 뇌염 바이러스 유전자 피알엠이을 발현하는 돼지 아데노바이러스 3형 有权
    重组蛋氨酸腺病毒3型表达日本ENCEPHALITIS病毒基因疫苗及其用途

    公开(公告)号：KR1020040047485A

    公开(公告)日：2004-06-05

    申请号：KR1020020075722

    申请日：2002-11-30

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 차상호 ,  박종현 ,  송재영 ,  안동준 ,  최은진 ,  김종염 ,  권준헌

    IPC: C12N15/861

    CPC classification number: C12N15/861 ,  C07K14/005 ,  C12N2770/24034

    Abstract: PURPOSE: Recombinant porcine adenovirus type 3 expressing Japanese encephalitis virus gene prME and use thereof are provided, thereby inducing immunity to Japanese encephalitis virus in human, and improving stability of the vaccine by using adenovirus. CONSTITUTION: A recombinant vector of porcine adenovirus type 3 expressing Japanese encephalitis virus gene prME, pPAV3E3-prME(KCTC 10376BP), is prepared by removing E3 gene of porcine adenovirus type 3, and inserting the Japanese encephalitis virus gene prME under the control of E3 promoter, wherein the Japanese encephalitis virus gene prME has the nucleotide sequence set forth in SEQ ID NO: 1. A host cell transformed with the recombinant vector pPAV3E3-prME(KCTC 10376BP) is provided. The recombinant porcine adenovirus type 3 expressing Japanese encephalitis virus gene prME(PAV3E3-prME) is obtained by culturing the transformed host cell. A vaccine for Japanese encephalitis virus comprises the recombinant porcine adenovirus type 3 expressing Japanese encephalitis virus gene prME.

    Abstract translation: 目的：提供3型表达日本脑炎病毒基因prME的重组猪腺病毒及其用途，从而诱导人体对日本脑炎病毒的免疫，并通过使用腺病毒提高疫苗的稳定性。 构成：通过除去3型猪腺病毒的E3基因，并将日本脑炎病毒基因prME置于E3的控制下，制备3型表达日本脑炎病毒基因prME，pPAV3E3-prME（KCTC 10376BP）的猪腺病毒重组载体 启动子，其中日本脑炎病毒基因prME具有SEQ ID NO：1所示的核苷酸序列。提供用重组载体pPAV3E3-prME（KCTC 10376BP）转化的宿主细胞。 通过培养转化的宿主细胞获得表达日本脑炎病毒基因prME（PAV3E3-prME）的重组猪腺病毒3型。 日本脑炎病毒疫苗包含3型表达日本脑炎病毒基因prME的重组猪腺病毒。

    일본 뇌염 바이러스 및 돼지 면역조절 유전자를 이용한 디엔에이 백신 및 그의 사용방법
    105.
    发明公开
    일본 뇌염 바이러스 및 돼지 면역조절 유전자를 이용한 디엔에이 백신 및 그의 사용방법 有权
    使用日本ENCEPHALITIS病毒和PORCINE免疫原性基因的DNA疫苗及其使用方法

    公开(公告)号：KR1020040047483A

    公开(公告)日：2004-06-05

    申请号：KR1020020075720

    申请日：2002-11-30

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 박종현 ,  차상호 ,  송재영 ,  안동준 ,  최은진 ,  김종염 ,  권준헌

    IPC: C12N15/86

    CPC classification number: A61K39/12 ,  A61K39/0005 ,  A61K2039/53 ,  A61K2039/552

    Abstract: PURPOSE: A DNA vaccine using Japanese encephalitis virus and porcine immunoregulatory genes, and a method for using the same are provided, which DNA vaccine selectively uses strong cellular immunity and humoral immunity by change of genetic manipulation or administration pathway, minimizes side-effects by diluents in the administration area, inhibits pathogenicity recovering shown in live vaccine, and minimizes lowering of immunity when it is used with other disease vaccines. CONSTITUTION: The DNA vaccine using Japanese encephalitis virus and porcine immunoregulatory genes comprises a recombinant plasmid pSLIA-prME(KCTC 10378BP) containing Japanese encephalitis virus gene prME and a recombinant plasmid pSLIA-NS1(KCTC 10379BP) containing porcine immunoregulatory gene NS1, and further comprises a recombinant plasmid pSLIA-PIL-2(KCTC 10380) containing porcine interleukin-2 gene PIL-2, wherein the DNA vaccine is subcutaneously administered to pigs through the pig's tail. The method for preventing the infection of Japanese encephalitis virus comprises administering the recombinant plasmids pSLIIA-prME and pSLIA-NS1 into pigs, wherein the recombinant plasmid pSLIA-PIL-2 may be further administered into pigs.

    Abstract translation: 目的：提供使用日本脑炎病毒和猪免疫调节基因的DNA疫苗及其使用方法，该DNA疫苗通过改变遗传操作或给药途径选择性地使用强的细胞免疫和体液免疫，从而最小化稀释剂的副作用 在管理区域，抑制活疫苗中显示的致病性恢复，并且当与其他疾病疫苗一起使用时，可以最大限度地降低免疫力。 构成：使用日本脑炎病毒和猪免疫调节基因的DNA疫苗包含含有日本脑炎病毒基因prME的重组质粒pSLIA-prME（KCTC 10378BP）和含有猪免疫调节基因NS1的重组质粒pSLIA-NS1（KCTC 10379BP），并且还包含 包含猪白细胞介素-2基因PIL-2的重组质粒pSLIA-PIL-2（KCTC10380），其中将DNA疫苗通过猪尾皮皮下施用于猪。 防止日本脑炎病毒感染的方法包括将重组质粒pSLIIA-prME和pSLIA-NS1投入猪，其中可以将重组质粒pSLIA-PIL-2进一步施用于猪。

    유전자재조합 바큘로바이러스 및 이에 의한 재조합 돼지전염성위장염바이러스 스파이크 단백질을 이용한 돼지전염성위장염바이러스 항체 검출을 위한 효소면역 검사방법
    106.
    发明授权
    유전자재조합 바큘로바이러스 및 이에 의한 재조합 돼지전염성위장염바이러스 스파이크 단백질을 이용한 돼지전염성위장염바이러스 항체 검출을 위한 효소면역 검사방법 有权
    重组杆状病毒和使用重组TGEV SPIKE蛋白的MACELISA检测特异性抗体对TGEV的检测

    公开(公告)号：KR100267744B1

    公开(公告)日：2000-11-01

    申请号：KR1019980000446

    申请日：1998-01-10

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 탁동섭 ,  권준헌 ,  김병한 ,  송재영 ,  강신영 ,  안수환

    IPC: C12N15/85 ,  C12N15/33

    Abstract: PURPOSE: Provided are a genetic recombinant baculovirus and a recombinant spike protein of transmissible gastroenteritis virus(TGEV) manufactured therefrom. And a monoclonal antibody capture enzyme linked immunosorbent assay for detecting the antibody of TGEV using the same protein is also provided, thereby the antibody of TGEV can be rapidly detected. CONSTITUTION: The recombinant spike protein of TGEV is produced by the steps of: isolating and identifying TGEV from pigs died of diarrhea; analyzing the nucleotide sequence of spike protein of TGEV; inserting the TGEV spike gene into the baculovirus gene to produce a recombinant baculovirus(KFCC-11015); infecting the recombinant baculovirus into SF9 cells to express TGEV spike protein. The antibody of TGEV is produced by inoculating the expressed recombinant TGEV spike protein into Guinea pigs. The monoclonal antibody capture enzyme linked immunosorbent assay using TGEV spike protein that is produced from the recombinant baculovirus(KFCC-11015) in Guinea pigs as an antigen is used in detecting the neutralizing antibody of TGEV.

    Abstract translation: 目的：提供遗传重组杆状病毒和由其制备的传染性胃肠炎病毒（TGEV）的重组穗蛋白。 还提供了使用相同蛋白质检测TGEV抗体的单克隆抗体捕获酶联免疫吸附测定法，从而可以快速检测TGEV抗体。 构成：TGEV的重组穗蛋白是通过以下步骤制备的：从腹泻死亡的猪中分离和鉴定TGEV; 分析TGEV刺突蛋白的核苷酸序列; 将TGEV刺突基因插入杆状病毒基因以产生重组杆状病毒（KFCC-11015）; 将重组杆状病毒感染到SF9细胞中以表达TGEV穗蛋白。 通过将表达的重组TGEV穗蛋白接种到豚鼠中来产生TGEV抗体。 使用从作为抗原的豚鼠中的重组杆状病毒（KFCC-11015）产生的TGEV尖峰蛋白的单克隆抗体捕获酶联免疫吸附测定用于检测TGEV的中和抗体。

    돼지 유행성설사병 바이러스의 특이 중화항체 검출방법
    109.
    发明公开
    돼지 유행성설사병 바이러스의 특이 중화항체 검출방법 有权
    猪腹泻病毒中特异性中和抗体的检测

    公开(公告)号：KR1019990065235A

    公开(公告)日：1999-08-05

    申请号：KR1019980000447

    申请日：1998-01-10

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 박종현 ,  송재영 ,  안동준 ,  현방훈 ,  차상호 ,  안수환 ,  권준헌

    IPC: C12N15/33

    Abstract: 본 발명은 돼지 유행성설사바이러스의 스파이크(spike) 단백질이 중화항체를 유발하므로 이 스파이크 유전자를 곤충 바이러스인 베큘로바이러스에 삽입하여 발현된 스파이크 단백질(한국종균협회 균주기탁번호 KFCC-11014)을 이용하여 돼지유행성설사병 바이러스의 특이 중화항체검출방법에 관한 발명이다. 유전자재조합 방법으로 대량 생산된 돼지 유행성 설사바이러스 spike 단백질을 이용하여 간접 효소면역학적 측정법(IS-ELISA)으로 돼지 유행성설사바이러스 중화항체를 검출하는 방법을 제공하는 발명이다.
    본 발명의 방법으로 보다 신속하고 정확하게 다수의 혈청을 사용하여 동시에 돼지 유행성설사바이러스 중화항체를 검사할 수 있다.

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