글리세롤 대사능이 조절된 숙주세포 및 이를 사용한 글리세롤 탈수효소 생산물의 생산방법
    101.
    发明公开
    글리세롤 대사능이 조절된 숙주세포 및 이를 사용한 글리세롤 탈수효소 생산물의 생산방법 无效
    具有调节的甘油代谢的主体细胞和使用其的糖苷脱氢酶代谢产物的制备方法

    公开(公告)号:KR1020140004401A

    公开(公告)日:2014-01-13

    申请号:KR1020120071903

    申请日:2012-07-02

    Inventor: 정원석 최인석

    Abstract: The present invention relates to a microorganism with adjusted glycerol metabolism, a method for manufacturing a glycerol dehydratase metabolite from glycerol using the same, and a method for manufacturing the microorganism, and more particularly, to a microorganism for inactivating a glycerol-3-phosphate regulon repressor gene (glpR) on a chromosome and for manufacturing a glycerol dehydratase metabolite from glycerol, a method for manufacturing a glycerol dehydratase metabolite such as 3-hydroxypropionic acid and/or 1,3-propanediol and the like using the same, and a method for manufacturing the microorganism. The microorganism with adjusted glycerol metabolism according to the present invention has the effects of noticeably increasing growth of the microorganism and production of the glycerol dehydratase metabolite using glycerol of low costs as a carbon source and an energy source, thereby, effectively producing a glycerol dehydratase metabolite such as 3-hydroxypropionic acid and/or 1,3-propanediol and the like using the same.

    Abstract translation: 本发明涉及具有调整甘油代谢的微生物,使用其从甘油制备甘油脱水酶代谢物的方法和微生物的制备方法,更具体地涉及用于灭活甘油-3-磷酸调节子的微生物 染色体上的抑制基因(glpR)和用于从甘油制造甘油脱水酶代谢物的甘油脱水酶基因(glpR),使用其制备甘油脱水酶代谢物如3-羟基丙酸和/或1,3-丙二醇等的方法,以及方法 用于制造微生物。 根据本发明的具有调整的甘油代谢的微生物具有使用低成本的甘油作为碳源和能量源,微生物生长显着增加和甘油脱水酶代谢物的产生的效果,从而有效地产生甘油脱水酶代谢物 例如使用它们的3-羟基丙酸和/或1,3-丙二醇等。

    PCR 데이터 및 다른 데이터에서 크로스토크의 영향을 보상하여 핵산을 분석하는 방법 및 장치
    102.
    发明公开
    PCR 데이터 및 다른 데이터에서 크로스토크의 영향을 보상하여 핵산을 분석하는 방법 및 장치 审中-实审
    通过在PCR和其他数据中补充交叉效应来分析核酸的方法和装置

    公开(公告)号:KR1020140002241A

    公开(公告)日:2014-01-08

    申请号:KR1020120070234

    申请日:2012-06-28

    Abstract: For a method of analyzing nucleic acid by compensating effect of a cross-talk in PCR and other data, using the change in fluorescence intensity detected from a different fluorescent dye labeled in the nucleic acid; the present method revises the cross-talk signal caused by each fluorescent dye. By compensating the change in fluorescence intensity detected from the compensated cross-talk signals, the final threshold values corresponding to the each fluorescent dye are estimated. [Reference numerals] (10) Nucleic acid analysis device; (110) Measuring unit; (120) Calculation unit; (130) Determination unit; (140) Concentration analysis unit; (150) Correction unit; (160) Estimating unit

    Abstract translation: 通过补偿PCR和其他数据中串扰的影响来分析核酸的方法,使用从核酸中标记的不同荧光染料检测到的荧光强度的变化; 本方法修改由每种荧光染料引起的串扰信号。 通过补偿从补偿的串扰信号检测的荧光强度的变化,估计对应于每个荧光染料的最终阈值。 (参考号)(10)核酸分析装置; (110)测量单元; (120)计算单位; (130)确定单位; (140)浓度分析单位; (150)校正单位; (160)估计单位

    형광 검출 광학계 및 이를 포함하는 다채널 형광 검출 장치
    109.
    发明公开
    형광 검출 광학계 및 이를 포함하는 다채널 형광 검출 장치 有权
    荧光检测光学系统和具有该荧光检测光学系统的多通道荧光检测装置

    公开(公告)号:KR1020120124227A

    公开(公告)日:2012-11-13

    申请号:KR1020110041994

    申请日:2011-05-03

    Abstract: PURPOSE: A fluorescence detection optical system and a multi-channel fluorescence detection apparatus including the same are provided to form constant focus location through an automatic focusing function even if a micro fluid element is not precisely arranged. CONSTITUTION: A first light source(101) emits excitation light of a first wavelength. A first excitation light filter(102) passes through the excitation light of the first wavelength. A first collimating lens(103) changes the excitation light of the first wavelength into a parallel beam. A second light source(104) emits excitation light of a second wavelength. A second excitation light filter(105) passes through the excitation light of the second wavelength. A second collimating lens(106) changes the excitation light of the second wavelength into a parallel beam. A first dichroic filter(107) transmits the excitation light of the first wavelength and reflects the excitation light of the second wavelength. A beam splitter(108) transmits a part of incident light and reflects the rest. A monitoring light detector(109) measures an amount of the excitation light transmitting the beam splitter. A second dichroic filter(110) reflects the excitation light and transmits fluorescence generated in a micro fluid element. [Reference numerals] (140) Focusing controller

    Abstract translation: 目的:提供荧光检测光学系统和包括该荧光检测光学系统的多通道荧光检测装置,以便即使微流体元件未被精确布置,也通过自动聚焦功能形成恒定的聚焦位置。 构成:第一光源(101)发射第一波长的激发光。 第一激发光滤光器(102)穿过第一波长的激发光。 第一准直透镜(103)将第一波长的激发光改变为平行光束。 第二光源(104)发射第二波长的激发光。 第二激发光滤光器(105)穿过第二波长的激发光。 第二准直透镜(106)将第二波长的激发光改变成平行光束。 第一分色滤光器(107)透射第一波长的激发光并反射第二波长的激发光。 分束器(108)透射入射光的一部分并反射其余部分。 监测光检测器(109)测量透射分束器的激发光的量。 第二分色滤光器(110)反射激发光并透射在微流体元件中产生的荧光。 (附图标记)(140)聚焦控制器

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