Abstract:
The present invention relates to a microorganism with adjusted glycerol metabolism, a method for manufacturing a glycerol dehydratase metabolite from glycerol using the same, and a method for manufacturing the microorganism, and more particularly, to a microorganism for inactivating a glycerol-3-phosphate regulon repressor gene (glpR) on a chromosome and for manufacturing a glycerol dehydratase metabolite from glycerol, a method for manufacturing a glycerol dehydratase metabolite such as 3-hydroxypropionic acid and/or 1,3-propanediol and the like using the same, and a method for manufacturing the microorganism. The microorganism with adjusted glycerol metabolism according to the present invention has the effects of noticeably increasing growth of the microorganism and production of the glycerol dehydratase metabolite using glycerol of low costs as a carbon source and an energy source, thereby, effectively producing a glycerol dehydratase metabolite such as 3-hydroxypropionic acid and/or 1,3-propanediol and the like using the same.
Abstract:
For a method of analyzing nucleic acid by compensating effect of a cross-talk in PCR and other data, using the change in fluorescence intensity detected from a different fluorescent dye labeled in the nucleic acid; the present method revises the cross-talk signal caused by each fluorescent dye. By compensating the change in fluorescence intensity detected from the compensated cross-talk signals, the final threshold values corresponding to the each fluorescent dye are estimated. [Reference numerals] (10) Nucleic acid analysis device; (110) Measuring unit; (120) Calculation unit; (130) Determination unit; (140) Concentration analysis unit; (150) Correction unit; (160) Estimating unit
Abstract:
PURPOSE: A fluorescence detection optical system and a multi-channel fluorescence detection apparatus including the same are provided to form constant focus location through an automatic focusing function even if a micro fluid element is not precisely arranged. CONSTITUTION: A first light source(101) emits excitation light of a first wavelength. A first excitation light filter(102) passes through the excitation light of the first wavelength. A first collimating lens(103) changes the excitation light of the first wavelength into a parallel beam. A second light source(104) emits excitation light of a second wavelength. A second excitation light filter(105) passes through the excitation light of the second wavelength. A second collimating lens(106) changes the excitation light of the second wavelength into a parallel beam. A first dichroic filter(107) transmits the excitation light of the first wavelength and reflects the excitation light of the second wavelength. A beam splitter(108) transmits a part of incident light and reflects the rest. A monitoring light detector(109) measures an amount of the excitation light transmitting the beam splitter. A second dichroic filter(110) reflects the excitation light and transmits fluorescence generated in a micro fluid element. [Reference numerals] (140) Focusing controller
Abstract:
PURPOSE: A gene analysis apparatus and a method for gene analysis using the same are provided to prevent contamination by foreign materials and to enhance accuracy and reliability. CONSTITUTION: A gene analysis apparatus comprises: a chamber(40) for crushing cells; a pump(40P) for pumping the crushed cells by a microchannel(40C); microchannels(50C,52C,54C,56C) for transferring amplification reagent into first to fourth PCR chambers(60,62,64,66); pumps(50P,52P,54P,56P) for pumping a PCR mixture to each microchannel; pumps(60P,62P,64P,66P) for injecting crushed cells and PCR mixture into the first to fourth PCR chambers; and a micro valves(40V,50V,60V) for controlling fluid flow.