公开(公告)号：KR1020040076387A

公开(公告)日：2004-09-01

申请号：KR1020030011718

申请日：2003-02-25

Applicant: 한국과학기술연구원

Inventor： 이강봉 ,  박현미 ,  김영만 ,  류재천

IPC: G01N30/00

Abstract: PURPOSE: Provided is an improved solid phase microextraction(SPME) fiber performing an extraction, concentration, and derivatization simultaneously thereon. A method for analyzing an alkylphenol and a bisphenol-A in a water sample by using the SPME fiber is also provided. CONSTITUTION: The SPME fiber is produced by soaking a SPME fiber in a mixture, comprising bis(trimethyl silyl)trifluoro acetamide(BSTFA) and trimethyl chloro silane(TMCS) in the weight ratio of 100:1, for 30min to 2hrs to absorb the BSTFA into the SPME fiber. And the method for analyzing the alkylphenol and the bisphenol-A comprises the steps of: bring the SPME fiber into contact with the water sample to absorb the alkylphenol and the bisphenol-A into the fiber; separating the fiber and then analyzing by using a gas chromatography/mass spectrometer(GC/MS).

Abstract translation: 目的：提供一种改进的固相微萃取（SPME）纤维，在其上同时进行萃取，浓缩和衍生。 还提供了通过使用SPME纤维分析水样品中的烷基酚和双酚A的方法。 构成：SPME纤维通过将SPME纤维以100（重量比）的双（三甲基甲硅烷基）三氟乙酰胺（BSTFA）和三甲基氯硅烷（TMCS））混合在一起混合30分钟至2小时来吸收 BSTFA进入SPME纤维。 并且分析烷基酚和双酚A的方法包括以下步骤：使SPME纤维与水样接触以将烷基酚和双酚A吸收到纤维中; 分离纤维，然后使用气相色谱/质谱仪（GC / MS）进行分析。

公开(公告)号：KR1020020026684A

公开(公告)日：2002-04-12

申请号：KR1020000057916

申请日：2000-10-02

Applicant: 한국과학기술연구원

Inventor： 정찬성 ,  이소하 ,  임대식 ,  신평균 ,  이강봉

IPC: C12P41/00

Abstract: PURPOSE: Provided is an effective resolution method of chiral compounds containing hydroxy group or ester group, by using lipase produced by Burkholderia Cocovenenans SY-01I(KFCC 11111). CONSTITUTION: Chiral compounds(formula 1, 2, 3 4 and 5) containing hydroxy group or ester group are resolved by hydrolyzing and trans-esterifying using the solid lipase prepared by dialysis or lyophilizing, wherein the hydrolysis is carried out at 5-35deg.C with by phosphate buffer solution of pH7.09; and the trans-esterification is conducted at 0-55 deg.C with organic solvent and an acylating agent. The organic solvent is selected from n-hexane, ethylacetate, benzene, toluene, THF, diethylether, acetonitrile, dichloroethane, dichloromethane, 1,4-dioxane, methanol, DMSO, DMF and hexane/ethylacetate(9/1 -5/5) and the above acylating agent is chosen from vinylacetate, isopropenylacetate, anhydrous acetic acid, anhydrous propionic acid and anhydrous butyric acid.

Abstract translation: 目的：通过使用伯克霍尔德氏菌SY-01I（KFCC 11111）生产的脂肪酶，提供含有羟基或酯基的手性化合物的有效解决方法。 构成：使用通过透析或冻干制备的固体脂肪酶进行水解和酯交换，分解含有羟基或酯基的手性化合物（式1,2,3,4,5），其中水解进行5-35度。 C用磷酸盐缓冲溶液pH7.09; 并且酯交换在0-55℃下与有机溶剂和酰化剂进行。 有机溶剂选自正己烷，乙酸乙酯，苯，甲苯，THF，二乙醚，乙腈，二氯乙烷，二氯甲烷，1,4-二恶烷，甲醇，DMSO，DMF和己烷/乙酸乙酯（9/1 -5/5） 并且上述酰化剂选自乙酸乙烯酯，异丙烯基乙酸酯，无水乙酸，无水丙酸和无水丁酸。

公开(公告)号：KR100275790B1

公开(公告)日：2000-12-15

申请号：KR1019980022953

申请日：1998-06-18

Applicant: 한국과학기술연구원

Inventor： 이강봉 ,  박현미 ,  김영만

IPC: B01D53/02 ,  B01D53/04

Abstract: 게 껍질 분말로 이루어진 유독한 휘발성 유기 화합물 농축용 흡착제 및 그의 제조방법. 본 발명의 게 껍질 흡착제는 특히 대기 중 휘발성 유기 화합물의 기체 크로마토그래피 분석에 앞서 이들 유기 화합물을 농축시키는데 특히 유용하게 이용될 수 있다. 본 발명의 흡착제는 자연 중에 산재해 있는 게 껍질을 원료로 함에 따라 제조 비용이 극히 저렴하면서도 검출한계 및 다양한 유기화합물 분석에의 활용도 측면에서도 기존의 흡착제에 비해 손색이 없고 사용방법도 간편하여 산업적으로 매우 유용하다.