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公开(公告)号：KR101327984B1
公开(公告)日：2013-11-13
申请号：KR1020110102636
申请日：2011-10-07
Applicant: 한국화학연구원
Abstract: 본 발명은 조류 바이오매스 생산과 품질의 동시증진을 위한 화합물 이용방법에 관한 것으로, 보다 상세하게는 식물 정유를 포함한 천연물을 유효성분으로 하는 조류 바이오매스의 생산 및 품질 동시 증진용 또는 위해성(危害性) 조류 방제용 조성물 및 상기 조성물을 이용한 방제방법으로, 상기 조성물 및 이를 이용한 방법은 유해 또는 경쟁 생물의 제어, 목표 조류 종의 생장촉진, 세포내 유용성분 함량 축적 유도 등을 동시에 가능케 함으로써 고품질 조류 바이오매스의 대량 확보에 적용할 수 있어, 미래 환경과 석유자원 고갈을 대비한 바이오매스 산업에 크게 기여할 것이다.
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公开(公告)号：KR1020130107686A
公开(公告)日：2013-10-02
申请号：KR1020120029656
申请日：2012-03-23
Applicant: 한국화학연구원
Abstract: PURPOSE: A method for producing bioethanol from Hydrodictyon family algae is provided to stably supply resources without moral problems and a sharp rise in food prices using algae when industrial demands for biomass increase. CONSTITUTION: A method for producing bioethanol from Hydrodictyon family algae comprises the steps of: saccharifying Hydrodictyon family algae and preparing a saccharified liquid containing monosaccharides; and culturing microorganisms using the saccharified liquid as a carbon source and producing bioethanol. The Hydrodictyon family algae are selected among Hydrodictyon sp., Pediastrum sp., and Sorastrum sp. The saccharification is performed using a hydrolytic catalyst, a polysaccharide decomposing enzyme, or a mixture thereof. [Reference numerals] (AA) Consecutive direct saccharifying/ fermenting process; (BB) Hydrodictyon family algae and algae biomass; (CC,GG,NN) Saccharification enzyme; (DD) Saccharifying; (EE) 40-600°C, 1 day; (FF) Algae biomass; (HH,OO) Ethanol producing strain; (II) Fermenting; (JJ,QQ) 30-40°C, 3 days; (KK,RR) Bio ethanol; (LL) Simultaneous direct saccharifying/ fermenting process; (MM) Hydrodictyon family algae and algae biomass (injected one time or two or more time); (PP) Saccharifying/fermenting
Abstract translation: 目的：提供一种从Hydrodictyon家族藻类生产生物乙醇的方法，以便在生物质工业需求增加时，稳定地供应没有道德问题的资源和使用藻类的食品价格大幅上涨。 构成：从Hydrodictyon家族藻类生产生物乙醇的方法包括以下步骤：糖化Hydrodictyon家族藻类并制备含有单糖的糖化液; 并使用糖化液体作为碳源培养微生物并生产生物乙醇。 Hydrodictyon家族藻类选自Hydrodictyon sp。，Pediastrum sp。和Sorastrum sp。 糖化使用水解催化剂，多糖分解酶或其混合物进行。 （附图标记）（AA）连续直接糖化/发酵过程; （BB）Hydrodictyon家庭藻类和藻类生物量; （CC，GG，NN）糖化酶; （DD）糖化; （EE）40-600℃，1天; （FF）藻类生物量; （HH，OO）乙醇生产菌株; （二）发酵; （JJ，QQ）30-40℃，3天; （KK，RR）生物乙醇; （LL）同时直接糖化/发酵过程; （MM）Hydrodictyon家庭藻类和藻类生物量（注射一次或两次或更多次）; （PP）糖化/发酵
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103.发明授权
公开(公告)号：KR101270255B1
公开(公告)日：2013-05-31
申请号：KR1020100093179
申请日：2010-09-27
Applicant: 한국화학연구원
Abstract: 본발명은아나카딕산을유효성분으로하는유해조류방제용조성물및 이를이용한유해조류방제방법에관한것으로, 상기본 발명에따른유해조류방제용조성물은유익한조류에는독성이적으면서남조류등의유해조류를상대적으로잘 방제하며각각의단독처리보다는혼합처리시 활성이상승됨으로써적은량의투입이가능하여환경에미치는부작용이보다경감될수 있으며, 조류(algae) 생장에있어서서로다른반응성을보여방제하고자목표하는유해조류를선택적으로제어할수 있는장점이있어식생을크게파괴하지않는범위에서수생태계의합리적관리에유용하게사용될수 있는장점이있을뿐 아니라유용한특정조류를실내외재배시유해조류오염에의한피해를방지하고자하는곳에사용될수 있어고품질의조류바이오매스생산에도적용될수 있는장점이있다.
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公开(公告)号：KR1020120106154A
公开(公告)日：2012-09-26
申请号：KR1020110024059
申请日：2011-03-17
Applicant: 한국화학연구원
Abstract: PURPOSE: A method for preparing hydrolysate highly containing glucose from algae biomass is provided to prevent sharp rise of food price and to enable easy biochemical/chemical saccharification. CONSTITUTION: A method for preparing hydrolysate highly containing glucose comprises: a step of hydrolyzing and chemical-hydrolyzing family Hydrodictyaceae without pretreatment; a step of centrifuging or filtering the hydrolysate to collect supernatant; and a step of concentrating the supernatant by drying at low pressure or membrane permeability evaporation. The hydrolysis catalyst includes an acid catalyst including sulfuric acid, hydrochloric acid, bromic acid, nitric acid, acetic acid, formic acid, phosphoric acid, para-toluene sulfonic acid(PTSA) or solid acid, or an alkaline catalyst including potassium hydroxide, sodium hydroxide, calcium hydroxide, or ammonium solution. [Reference numerals] (AA) Collected family Hydrodictyaceae and algae biomass; (BB) Extracted available substances(physiologically active substances, lipid, etc); (CC) Pre-extraction of available substances using water or solvent; -Pulverizing, drying, sterilizing, etc; (DD) Collected family Hydrodictyaceae and algae biomass(living matter, dried material); -Solid powder of extracted algae; (EE) Saccharification with complex enzyme; Chemical hydrolysis; (FF) Hydrolysate of low concentration; (GG) Hydrolysate isolation, filtration, concentration(reduction of fermentation inhibitors); (HH) Saccharide concentrate of high quality(use in fermentation or chemical conversion)
Abstract translation: 目的：提供一种从藻类生物质制备高含量葡萄糖的水解产物的方法，以防止食品价格急剧上涨，从而实现简便的生化/化学糖化。 构成：一种制备高含葡萄糖的水解产物的方法，包括：水解和化学水解不含预处理的水杨酸科的步骤; 离心或过滤水解产物以收集上清液的步骤; 以及通过在低压或膜渗透性蒸发下干燥浓缩上清液的步骤。 水解催化剂包括酸催化剂，包括硫酸，盐酸，溴酸，硝酸，乙酸，甲酸，磷酸，对甲苯磺酸（PTSA）或固体酸，或碱性催化剂，包括氢氧化钾， 氢氧化物，氢氧化钙或铵溶液。 （附图标记）（AA）收集家庭水果科和藻类生物质; （BB）提取的有效物质（生理活性物质，脂质等）; （CC）使用水或溶剂预提取可用物质; - 粉碎，干燥，灭菌等; （DD）收集家庭Hydrodicty科和藻类生物量（生物，干物质）; 提取藻类固体粉末; （EE）用复合酶糖化; 化学水解; （FF）低浓度水解产物; （GG）水解产物分离，过滤，浓缩（减少发酵抑制剂）; （HH）高品质糖精（用于发酵或化学转化）
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公开(公告)号：KR1020120106136A
公开(公告)日：2012-09-26
申请号：KR1020110024033
申请日：2011-03-17
Applicant: 한국화학연구원
CPC classification number: C12P7/56
Abstract: PURPOSE: A method for producing lactic acid through simultaneously saccharification and fermentation of algae biomass is provided to stably and continuously supply source and to ensure high saccharification rate. CONSTITUTION: A method for producing lactic acid of high concentration from algae biomass comprises: a step of putting algae and saccharifying enzyme in a reactor and saccharifying at 20-60 Deg. C.; a step of continuously putting fermentation strain to the reactor; and a step of fermenting at 25-50 Deg. C. for 1-5 days. The algae include living algae, dried algae, residue prepared by leaching with alcohol of 1-5 carbon atoms, residue prepared after extraction with water, alcohol of 1-5 carbon atoms, or serial solvent thereof, and a mixture of residue prepared after extraction of lipid ingredients using organic solvent. [Reference numerals] (A) Continuous saccharifying/fermenting process(A process); (AA) Fermenting strain injection; (B) Simultaneous saccharifying/fermenting process(B process); (B1,E1) Algae biomass-saccharifying enzyme; (B2,E2) Algae biomass-saccharifying enzyme; (CC) Saccharification; (DD) 50°C 2 days; (FF) Fermentation; (GG,LL) 37°C 3 days; (HH,NN) Lactic acid; (II) Saccharifying enzyme and fermenting strain injection; (JJ) Algae biomass; (KK) Saccharifying/fermenting
Abstract translation: 目的：提供藻类生物质同时糖化发酵生产乳酸的方法，稳定，持续地供应来源，确保高糖化率。 构成：从藻类生物质生产高浓度乳酸的方法包括：将藻类和糖化酶置于反应器中并在20-60度进行糖化的步骤。 C。; 将发酵菌株连续放入反应器的步骤; 和25-50度发酵的步骤。 1-5天。 藻类包括生活藻类，干藻类，用1-5个碳原子的醇浸出的残渣，用水萃取后的残留物，1-5个碳原子的醇或其连续溶剂，以及提取后制备的残留物的混合物 的脂质成分使用有机溶剂。 （A）连续糖化/发酵方法（A方法）; （AA）发酵菌株注射; （B）同时糖化/发酵工艺（B工艺）; （B1，E1）藻类生物质糖化酶; （B2，E2）藻类生物质糖化酶; （CC）糖化; （DD）50°C 2天; （FF）发酵; （GG，LL）37℃3天; （HH，NN）乳酸; （二）糖化酶和发酵菌株注射液; （JJ）藻类生物量; （KK）糖化/发酵
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Patent Agency Ranking
公开(公告)号：KR1020100089956A
公开(公告)日：2010-08-13
申请号：KR1020090009160
申请日：2009-02-05
Applicant: 한국화학연구원
CPC classification number: A01N59/16 , A01N31/16 , A01N33/12 , A01N37/02 , A01N59/00 , A01N65/08 , A01N65/10 , A01N65/12 , A01N65/22 , A01N65/30 , A01N65/42 , B82Y30/00
Abstract: PURPOSE: A composition for preventing harmful algae containing nano silver is provided to ensure eco-friendly condition to human, animal, and environment. CONSTITUTION: A composition for preventing harmful algae contains nano silver as an active ingredient without nitrogen and phosphorus. The nano silver is silver metal or silver oxide of nano size. The nano silver is contained in 0.01-15 weight% based on total weight. A method for preparing the composition comprises: a step of dissolving silver salt in distilled water; a step of filtering and washing carbon salt or carbon gas; and a step of adding organic/inorganic reductant or persulfated salt and hydroxide to form silver salt in a nano size.
Abstract translation: 目的：提供一种防止含有纳米银的有害藻类的组合物，以确保对人类，动物和环境的环境友好状况。 构成：防止有害藻类的组合物含有纳米银作为活性成分，不含氮和磷。 纳米银是银金属或纳米尺寸的氧化银。 纳米银以总重量计为0.01-15重量％。 制备该组合物的方法包括：将银盐溶解在蒸馏水中的步骤; 过滤和洗涤碳盐或碳气的步骤; 以及添加有机/无机还原剂或过硫酸盐和氢氧化物以形成纳米尺寸的银盐的步骤。
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公开(公告)号：KR1020100041560A
公开(公告)日：2010-04-22
申请号：KR1020080100802
申请日：2008-10-14
Applicant: 한국화학연구원 , 경상대학교산학협력단
Abstract: PURPOSE: An antibiotics marker free transformed Festuca sp. plant is provided to improve an effect of preventing weeds and reduce environmental problem. CONSTITUTION: A method for producing an antibiotic marker-free herbicide complex resistant Festuca sp. plant comprises: a step of transforming Agrobacterium with a plant expression vector containing bar gene and CP4-EPSPS gene; a step of co-culturing the transformed Agrobacterium and Festuca sp. embryogenic callus; and a step of selecting transformed shoot from the Festuca sp. embryogenic callus. The Festuca sp. plant is Festuca arundinacea. The plant expression vector is p35S-EPSPS-B vector.
Abstract translation: 目的：抗生素标记自由转化的Festuca sp。 提供植物提高防止杂草的效果，减少环境问题。 构成：生产抗生素标记物除草剂复合物抗Festuca sp的方法。 植物包括：用含有bar基因和CP4-EPSPS基因的植物表达载体转化农杆菌的步骤; 共培养转化的土壤杆菌和Festuca sp的步骤。 胚性愈伤组织; 并从Festuca sp中选择转化的芽的步骤。 胚性愈伤组织。 Festuca sp。 植物是Festuca arundinacea。 植物表达载体为p35S-EPSPS-B载体。
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公开(公告)号：KR100911322B1
公开(公告)日：2009-08-07
申请号：KR1020070104641
申请日：2007-10-17
Applicant: 한국화학연구원
Abstract: 본 발명은 하기 화학식 1 또는 화학식 2의 폴리아세틸렌계 화합물 또는 이들 화합물 중 적어도 하나를 포함하는 식물 추출물을 유효성분으로 함유하는 유해 조류 방제용 조성물, 및 이를 이용한 유해 조류의 방제방법에 관한 것으로, 본 발명의 폴리아세틸렌계 화합물 및 이를 함유한 식물추출물은 남조류, 적조 등의 유해 조류에 대해 선택적인 생육억제효과를 나타내므로, 이를 함유하는 방제용 조성물은 환경 친화적이면서 효능이 우수한 유해 조류 방제제로서 유용하게 활용될 수 있다.
[화학식 1]
상기 식에서,
R
1 및
R
2 는 각각 독립적으로 H 또는 OH이다.
[화학식 2]
상기 식에서,
R
1 은 OAc, Cl 또는 OH이다.
유해조류, 남조류, 적조, 폴리아세틸렌계 화합물, 식물 추출물-
公开(公告)号：KR1020090070018A
公开(公告)日：2009-07-01
申请号：KR1020070137872
申请日：2007-12-26
Applicant: 한국화학연구원 , 동아대학교 산학협력단
CPC classification number: C12N15/8274 , C12N9/1092 , C12N15/8205 , C12N15/8209 , C12N15/8237 , C12N15/8275 , C12Y205/01019
Abstract: An antibiotics marker free soybean has herbicide complex resistance using bar gene and CP4-EPSPS gene is provided to improve the weed prevention effect. A method for manufacturing an antibiotics marker free bean which has herbicide complex resistance comprises: a step of transforming Agrobacterium with a plant expression vector p35S-EPSPS-B which contains bar gene and CP4-EPSPS (CP4 5-enolpyruvylshikimate-3-phosphate synthase) gene but not antibiotics marker gene; a step of co-culturing transformed Agrobacterium and bean; and a step of selecting transformed bean and cultivating.
Abstract translation: 抗生素无标记大豆具有使用bar基因的除草剂复合物抗性，并提供CP4-EPSPS基因以提高除草效果。 具有除草复合抗性的抗生素无标记豆的制造方法包括：用含有bar基因和CP4-EPSPS（CP4- 5-烯醇丙酮酸莽草酸-3-磷酸合成酶）的植物表达载体p35S-EPSPS-B转化土壤杆菌的步骤， 基因但不是抗生素标记基因; 共培养转化的土壤杆菌和豆类的步骤; 并选择转化豆和栽培的步骤。
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公开(公告)号：KR100621917B1
公开(公告)日：2006-09-14
申请号：KR1020040040484
申请日：2004-06-03
Applicant: 한국화학연구원
IPC: A01N65/42
Abstract: 본 발명은 둥굴레속(
Polygonatum ) 식물 또는 그 추출물을 유효성분으로 함유하는 유해조류 및 개구리밥 방제 조성물 및 이를 이용한 방제방법에 관한 것으로, 본 발명의 둥굴레속 식물 또는 그 추출물은 유익한 조류나 수생생물에는 무해하면서, 남조류 및 적조류 등의 유해조류에만 선택적으로 우수한 방제효능을 나타내고, 다른 방제활성을 가진 화합물들과 상가적 효과를 나타내므로, 이를 함유하는 방제 조성물은 자연친화적이면서 효능이 우수한 유해조류 방제제로서 유용하게 활용될 수 있다.
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