公开(公告)号：WO1997034138A1

公开(公告)日：1997-09-18

申请号：PCT/US1997004015

申请日：1997-03-14

Applicant: WASHINGTON UNIVERSITY ,  WILSON, Richard, K. ,  MARDIS, Elaine, R. ,  PANUSSIS, Dimitrios, A.

Inventor： WASHINGTON UNIVERSITY

IPC: G01N01/10

CPC classification number: B01L3/0293 ,  G01N27/44743 ,  G01N35/1065 ,  G01N2035/00237 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: A method of and apparatus for simultaneously and uniformly transferring micro quantities of liquid from a first plurality of separate cells to a second plurality of separate cells. The gel loading apparatus (20) comprises a vertical support plate (26), a pressure block (28) mounted on the support plate and defining a pressure block (28) mounted on the support plate and defining pressure chamber (30) therein, and a slidable pressure tray (32) engageable with an underside of the pressure block for closing the pressure chamber. A plurality of liquid conveying conduits (capillaries 34) have first ends (36) inside the pressure chamber (30) and second ends (38) outside the pressure chamber. The second ends (38) of the capillaries (34) are preferably arranged for insertion between the plates of glass (22, 24) and into destination wells (21).

Abstract translation: 一种用于同时且均匀地将微量液体从第一多个分离单元转移到第二多个分离单元的方法和装置。 凝胶加载装置（20）包括垂直支撑板（26），安装在支撑板上的压力块（28），并且限定安装在支撑板上并在其中限定压力室（30）的压力块（28），以及 可滑动的压力托盘（32）可与压力块的下侧接合，用于关闭压力室。 多个液体输送管道（毛细管34）在压力室（30）内部具有第一端（36），在压力室外部具有第二端（38）。 毛细管（34）的第二端（38）优选地布置成用于插入在玻璃板（22,24）之间并进入目的井（21）中。

公开(公告)号：WO1997022719A1

公开(公告)日：1997-06-26

申请号：PCT/US1996020379

申请日：1996-12-17

Applicant: WASHINGTON UNIVERSITY ,  KWOK, Pui-Yan ,  CHEN, Xiangning

Inventor： WASHINGTON UNIVERSITY

IPC: C12Q01/68

CPC classification number: C12Q1/6818 ,  C12Q2600/156 ,  C12Q2565/1015 ,  C12Q2561/125 ,  C12Q2535/125 ,  C12Q2525/186 ,  C12Q2563/107

Abstract: A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.

Abstract translation: 公开了一种用于检测核酸中靶核苷酸或核苷酸序列的存在的方法。 该方法包括在核酸靶位点上形成用两个荧光团标记的寡核苷酸。 通过将单标记的双脱氧核苷三磷酸加成到单标记的多核苷酸中或通过连接两个单一标记的多核苷酸来形成双重标记的寡核苷酸。 在变性时荧光共振能量转移的检测表明靶的存在。 还提供套件。 该方法特别适用于基因分型。

公开(公告)号：WO1997013843A1

公开(公告)日：1997-04-17

申请号：PCT/US1996016181

申请日：1996-10-09

Applicant: CORNELL RESEARCH FOUNDATION, INC. ,  WASHINGTON UNIVERSITY

Inventor： CORNELL RESEARCH FOUNDATION, INC. ,  WASHINGTON UNIVERSITY ,  WU, Ray, J. ,  HO, Tuan-Hua, D.

IPC: C12N05/00

CPC classification number: C07K14/415 ,  C12N15/8273

Abstract: The present invention is directed to a method of producing a cereal plant cell or protoplast useful for regeneration of a water stress or salt stress tolerant cereal plant by transforming the cereal plant cell or protoplast with a nucleic acid encoding a late embryogenesis abundant protein. A transgenic cereal plant or cereal plant cell or protoplast transformed with a nucleic acid encoding a late embryogenesis abundant protein is also provided. An LEA protein gene, HVA1, from barley (Hordeum vulgare L.) was transformed into rice (Oryza sativa L.) plants. The resulting transgenic rice plants accumulate the HVA1 protein in both leaves and roots. Transgenic rice plants showed significantly increased tolerance to water stress (drought) and salt stress.

Abstract translation: 本发明涉及通过用编码晚期胚胎发生丰富蛋白质的核酸转化谷物植物细胞或原生质体来生产可用于再生水分胁迫或耐盐胁迫谷物植物的谷物植物细胞或原生质体的方法。 还提供了用编码晚期胚胎发生丰富蛋白质的核酸转化的转基因谷物植物或谷物植物细胞或原生质体。 将来自大麦（Hordeum vulgare L.）的LEA蛋白基因HVA1转化到水稻（Oryza sativa L.）植物中。 所得转基因水稻在叶和根中积累HVA1蛋白。 转基因水稻对水胁迫（干旱）和盐胁迫的耐受性显着增加。

公开(公告)号：WO1997009976A2

公开(公告)日：1997-03-20

申请号：PCT/IB1996000981

申请日：1996-08-23

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  CHOI, Dennis, Wonkyu ,  KOH, Jae-young

IPC: A61K31/195

CPC classification number: A61K31/4745 ,  A61K31/17 ,  A61K31/198 ,  A61K31/325 ,  A61K31/422 ,  A61K31/44 ,  A61K31/444 ,  A61K31/47

Abstract: The invention relates to the use of pharmaceutically acceptable zinc chelating compounds for the manufacture of medicaments for the treatment of neurotoxic injury.

Abstract translation: 本发明涉及用于制造用于治疗神经毒素病症的药物的药学上可接受的锌螯合化合物。

公开(公告)号：WO1996040947A1

公开(公告)日：1996-12-19

申请号：PCT/US1996009774

申请日：1996-06-07

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  CURTISS, Roy, III ,  TINGE, Steven, A.

IPC: C12N15/64

CPC classification number: C12N15/64 ,  C12N15/70 ,  C12N15/74

Abstract: Disclosed in an Environmentally Limited Viability System (ELVS) for microorganisms based on differences between permissive and non-permissive environments. Viability of the microorganisms are limited to a permissive environment by specifically expressing one or more essential genes only in the permissive environment, and/or expressing one or more lethal genes only in the non-permissive environment. Temporary viability in a non-permissive environment can be achieved by temporarily expressing one or more essential genes in a non-permissive environment, and/or temporarily delaying expression of one or more lethal genes in the non-permissive environment. Environmentally Limited Viabilty Systems are also disclosed involving coordinate expression of a combination of required genes and lethal genes. Microorganisms containing an Environmentally Limited Viability System are useful for release into permissive and non-permissive environments. Temperature regulated Environmentally Limited Viability Systems are particularly suited for delivery of expression products, such as antigens, using recombinant avirulent Salmonella by limiting their growth to the warmer environment inside the host, or by allowing growth for only a limited time in the host.

Abstract translation: 基于许可和非允许环境之间的差异，在针对微生物的环境有限的生存力系统（ELVS）中披露。 通过仅在许可环境中特异性表达一个或多个必需基因，和/或仅在非允许环境中表达一个或多个致死基因，将微生物的活力限制在许可环境中。 通过在非允许环境中临时表达一种或多种必需基因，和/或在非允许环境中暂时延缓一种或多种致死基因的表达，可以实现非允许环境中的临时活力。 还公开了环境有限的维持性系统，涉及协调所需基因和致死基因组合的表达。 含有环境有限的生存能力系统的微生物对于释放到容许和不允许的环境中是有用的。 温度调节环境有限的生存能力系统特别适用于通过将其生长限制在主机内较暖的环境中，或通过在宿主中仅允许有限的时间生长，使用重组无毒力沙门氏菌来递送表达产物，如抗原。

公开(公告)号：WO1996038463A1

公开(公告)日：1996-12-05

申请号：PCT/US1996008235

申请日：1996-05-31

Applicant: WASHINGTON UNIVERSITY ,  WATSON, Mark, A. ,  FLEMING, Timothy, P.

Inventor： WASHINGTON UNIVERSITY

IPC: C07H21/04

CPC classification number: C07K14/47 ,  A61K38/00 ,  C12Q1/6886 ,  C12Q2600/112 ,  G01N33/57415

Abstract: A purified and isolated DNA sequence and the encoded mammary-specific protein, mammaglobin, are disclosed. Also disclosed are methods for the detecting breast cancer based upon the overexpression and secretion of mammaglobin by breast cancer cells. The methods detect and/or quantitate the presence of mammaglobin or the mRNA encoding mammaglobin.

Abstract translation: 公开了纯化和分离的DNA序列和编码的乳腺特异性蛋白，乳腺球蛋白。 还公开了基于乳腺癌细胞的乳腺球蛋白的过量表达和分泌来检测乳腺癌的方法。 该方法检测和/或定量乳腺球蛋白或编码乳腺珠蛋白的mRNA的存在。

公开(公告)号：WO1996037216A1

公开(公告)日：1996-11-28

申请号：PCT/US1996006704

申请日：1996-05-09

Applicant: GENENTECH, INC. ,  WASHINGTON UNIVERSITY

Inventor： GENENTECH, INC. ,  WASHINGTON UNIVERSITY ,  CLARK, Ross, G. ,  GESUNDHEIT, Neil ,  HAMMERMAN, Marc, R. ,  MILLER, Steven, B.

IPC: A61K38/30

CPC classification number: A61K38/30 ,  A61K38/556 ,  A61K38/2242 ,  A61K38/27 ,  A61K2300/00

Abstract: A method is disclosed that comprises administering insulin-like growth factor-I (IGF-I) to a mammal so as to sustain its biological activity in the mammal comprising administering a therapeutically effective amount of IGF-I to the mammal to provide an exposure to IGF-I for a period of time that stimulates the maximum biological response in the mammal, then discontinuing said administration for a period of time equal to or less than the time period used for administration, and repeating this pattern of administration and discontinuance of administration for a period as long as necessary to achieve or maintain the desired biological response in the mammal.

Abstract translation: 公开了一种方法，其包括向哺乳动物施用胰岛素样生长因子-I（IGF-I）以维持其在哺乳动物中的生物学活性，包括向哺乳动物施用治疗有效量的IGF-1以提供暴露于 IGF-I刺激哺乳动物最大生物反应的一段时间，然后停止所述施用一段时间等于或小于用于给药的时间段，并重复这种给药方式和中断给药方式 只要在哺乳动物中达到或维持所需生物反应所必需的时间。

公开(公告)号：WO1996033488A1

公开(公告)日：1996-10-24

申请号：PCT/US1996004914

申请日：1996-04-09

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  INDECK, Ronald, S.

IPC: G11B05/584

CPC classification number: G11B5/488 ,  G11B5/09 ,  G11B5/5534 ,  G11B5/58 ,  G11B5/59633

Abstract: A vernier magnetic recording head (30) comprises multiple read and write transducers. The write transducers (32, 34, 36, 38 and 40) are equally spaced a first distance apart and the read transducers (42, 44, 46, 48, 50 and 51) are equally spaced a second distance apart. The write transducers initially write a plurality of equally spaced servo tracks (52, 54, 56, 58 and 60) which can be subsequently tracked by the read transducers. The write transducers may then be used to successively write a plurality of data tracks (62, 64, 66, 68 and 70) to one side of each servo track. The read transducers are positioned relative to the write transducers such that one read transducer is always positioned to track a servo track while the write transducers are writing the data tracks, thereby positioning the data tracks equidistant and parallel to or concentric with the servo tracks. One read transducer is positioned to track a servo track as the head is positioned at an extreme so that read transducers read data from the furthest data tracks, and the head may be positioned to write an adjacent set of new servo tracks.

Abstract translation: 游标磁记录头（30）包括多个读和写传感器。 写换能器（32,34,36,38和40）间隔开第一距离，并且读换能器（42,44,46,48,50和51）间隔开第二距离。 写入换能器最初写入多个等间距的伺服轨道（52,54,56,58和60），随后可以由读取的换能器跟踪。 然后可以使用写换能器来连续地将多个数据轨道（62,64,66,68和70）写入每个伺服轨道的一侧。 读取换能器相对于写入换能器定位，使得一个读取换能器总是被定位成跟踪伺服轨迹，同时写入换能器正在写入数据轨道，从而将数据轨迹等距并平行于或与伺服轨道同心。 一个读取传感器定位成当头部位于极端时跟踪伺服轨迹，以便读取传感器从最远的数据轨道读取数据，并且头部可被定位成写入相邻的一组新的伺服轨迹。

公开(公告)号：WO1996028730A1

公开(公告)日：1996-09-19

申请号：PCT/US1996002693

申请日：1996-03-13

Applicant: WASHINGTON UNIVERSITY ,  ORNITZ, David, M.

Inventor： WASHINGTON UNIVERSITY

IPC: G01N33/50

CPC classification number: G01N33/5011 ,  G01N33/5008 ,  G01N33/502 ,  G01N2333/61 ,  G01N2333/71

Abstract: Disclosed are fibroblast growth factor (FGF) binding and FGF receptor activation, and a method of identifying small molecular weight compounds that interact with FGF to modulate its activity such as, e.g., activators and inhibitors. Illustrative small oligosaccharides, namely di- and tri-saccharides, are shown to be effective modulators of FGF binding and FGF receptor activation.

Abstract translation: 公开了成纤维细胞生长因子（FGF）结合和FGF受体活化，以及鉴定与FGF相互作用以调节其活性的小分子量化合物的方法，例如活化剂和抑制剂。 示例性的小寡糖，即二糖和三糖被证明是FGF结合和FGF受体活化的有效调节剂。

公开(公告)号：WO1996013614A1

公开(公告)日：1996-05-09

申请号：PCT/US1995014246

申请日：1995-10-31

Applicant: WASHINGTON UNIVERSITY ,  KORSMEYER, Stanley, J.

Inventor： WASHINGTON UNIVERSITY

IPC: C12Q01/68

CPC classification number: C07K14/82 ,  A61K38/00 ,  C07K14/4747 ,  C07K2319/00

Abstract: The invention provides novel compositions, methods, and uses of Bad polypeptides and polynucleotides.

Abstract translation: 本发明提供了坏多肽和多核苷酸的新型组合物，方法和用途。