-
公开(公告)号：WO2002030457A3
公开(公告)日：2002-04-18
申请号：PCT/US2001/031606
申请日：2001-10-11
Applicant: WASHINGTON UNIVERSITY IN ST. LOUIS , MEGAN HEALTH, INC. , CURTISS, Roy, III , TINGE, Steven, A.
Inventor： CURTISS, Roy, III , TINGE, Steven, A.
IPC: C12N1/36
Abstract: Compositions comprising a microbe having an attenuating mutation comprising a recombinant transcription terminator insertion in a chromosomal gene are disclosed. The compositions can be used as vaccines or carrier vehicles for delivering a desired protein to an individual. Also disclosed are methods for immunizing an individual and methods of delivering a desired gene product to an individual based upon administration of the compositions.
-
公开(公告)号：WO1996040947A1
公开(公告)日：1996-12-19
申请号：PCT/US1996009774
申请日：1996-06-07
Applicant: WASHINGTON UNIVERSITY
Inventor： WASHINGTON UNIVERSITY , CURTISS, Roy, III , TINGE, Steven, A.
IPC: C12N15/64
Abstract: Disclosed in an Environmentally Limited Viability System (ELVS) for microorganisms based on differences between permissive and non-permissive environments. Viability of the microorganisms are limited to a permissive environment by specifically expressing one or more essential genes only in the permissive environment, and/or expressing one or more lethal genes only in the non-permissive environment. Temporary viability in a non-permissive environment can be achieved by temporarily expressing one or more essential genes in a non-permissive environment, and/or temporarily delaying expression of one or more lethal genes in the non-permissive environment. Environmentally Limited Viabilty Systems are also disclosed involving coordinate expression of a combination of required genes and lethal genes. Microorganisms containing an Environmentally Limited Viability System are useful for release into permissive and non-permissive environments. Temperature regulated Environmentally Limited Viability Systems are particularly suited for delivery of expression products, such as antigens, using recombinant avirulent Salmonella by limiting their growth to the warmer environment inside the host, or by allowing growth for only a limited time in the host.
Abstract translation: 基于许可和非允许环境之间的差异，在针对微生物的环境有限的生存力系统（ELVS）中披露。 通过仅在许可环境中特异性表达一个或多个必需基因，和/或仅在非允许环境中表达一个或多个致死基因，将微生物的活力限制在许可环境中。 通过在非允许环境中临时表达一种或多种必需基因，和/或在非允许环境中暂时延缓一种或多种致死基因的表达，可以实现非允许环境中的临时活力。 还公开了环境有限的维持性系统，涉及协调所需基因和致死基因组合的表达。 含有环境有限的生存能力系统的微生物对于释放到容许和不允许的环境中是有用的。 温度调节环境有限的生存能力系统特别适用于通过将其生长限制在主机内较暖的环境中，或通过在宿主中仅允许有限的时间生长，使用重组无毒力沙门氏菌来递送表达产物，如抗原。
-
公开(公告)号：EP1292687B1
公开(公告)日：2006-08-16
申请号：EP01944119.5
申请日：2001-04-30
Applicant: Washington University , AVANT Immunotherapeutics, Inc.
Inventor： CURTISS, Roy, III , TINGE, Steven, A.
CPC classification number: C12N15/74 , A61K2039/523 , A61K2039/53 , C12N15/635
Abstract: We describe a regulated antigen delivery system (RADS) that has (a) a vector that includes (1) a gene encoding a desired gene product operably linked to a control sequence, (2) an origin of replication conferring vector replication using DNA polymerase III, and (3) an origin of replication conferring vector replication using DNA polymerase I, where the second origin of replication is operably linked to a control sequence that is repressible by a repressor. The RADS microorganism also has a gene encoding a repressor, operably linked to an activatible control sequence. The RADS described provide high levels of the desired gene product after repression of the high copy number origin of replication is lifted. The RADS are particularly useful as live bacterial vaccines. Also described is a delayed RADS system, in which there is a delay before the high copy number origin is expressed after the repression is lifted. The delayed RADS is also particularly useful for live bacterial vaccines. Also described are several control elements useful for these systems, as well as methods for providing immunity to a pathogen in a vertebrate immunized with the RADS microorganisms.
-
Patent Agency Ranking
公开(公告)号：EP1292687A2
公开(公告)日：2003-03-19
申请号：EP01944119.5
申请日：2001-04-30
Applicant: WASHINGTON UNIVERSITY , Megan Health, Inc.
Inventor： CURTISS, Roy, III , TINGE, Steven, A.
CPC classification number: C12N15/74 , A61K2039/523 , A61K2039/53 , C12N15/635
Abstract: We describe a regulated antigen delivery system (RADS) that has (a) a vector that includes (1) a gene encoding a desired gene product operably linked to a control sequence, (2) an origin of replication conferring vector replication using DNA polymerase III, and (3) an origin of replication conferring vector replication using DNA polymerase I, where the second origin of replication is operably linked to a control sequence that is repressible by a repressor. The RADS microorganism also has a gene encoding a repressor, operably linked to an activatible control sequence. The RADS described provide high levels of the desired gene product after repression of the high copy number origin of replication is lifted. The RADS are particularly useful as live bacterial vaccines. Also described is a delayed RADS system, in which there is a delay before the high copy number origin is expressed after the repression is lifted. The delayed RADS is also particularly useful for live bacterial vaccines. Also described are several control elements useful for these systems, as well as methods for providing immunity to a pathogen in a vertebrate immunized with the RADS microorganisms.
-
公开(公告)号：EP0832255B1
公开(公告)日：2005-12-14
申请号：EP96919292.1
申请日：1996-06-07
Applicant: Washington University , AVANT Immunotherapeutics, Inc.
Inventor： CURTISS, Roy, III , TINGE, Steven, A.
IPC: C12N15/64 , A61K39/108 , A61K39/112 , C12N15/70 , C12N15/74 , C12N1/21
Abstract: Disclosed in an Environmentally Limited Viability System (ELVS) for microorganisms based on differences between permissive and non-permissive environments. Viability of the microorganisms are limited to a permissive environment by specifically expressing one or more essential genes only in the permissive environment, and/or expressing one or more lethal genes only in the non-permissive environment. Temporary viability in a non-permissive environment can be achieved by temporarily expressing one or more essential genes in a non-permissive environment, and/or temporarily delaying expression of one or more lethal genes in the non-permissive environment. Environmentally Limited Viabilty Systems are also disclosed involving coordinate expression of a combination of required genes and lethal genes. Microorganisms containing an Environmentally Limited Viability System are useful for release into permissive and non-permissive environments. Temperature regulated Environmentally Limited Viability Systems are particularly suited for delivery of expression products, such as antigens, using recombinant avirulent Salmonella by limiting their growth to the warmer environment inside the host, or by allowing growth for only a limited time in the host.
-
6.发明授权MICROBES HAVING AN ATTENUATING MUTATION COMPRISING A TRANSCRIPTION TERMINATOR 有权与转录漏失含STEAMING突变GERMS
公开(公告)号：EP1326960B1
公开(公告)日：2004-12-08
申请号：EP01979646.5
申请日：2001-10-11
Applicant: Washington University in St. Louis , Megan Health, Inc. , Curtiss, Roy III , Tinge, Steven A.
Inventor： CURTISS, Roy, III , TINGE, Steven, A.
CPC classification number: C12N15/74 , A61K39/0006 , A61K2039/522 , A61K2039/53 , Y02A50/407
Abstract: Compositions comprising a microbe having an attenuating mutation comprising a recombinant transcription terminator insertion in a chromosomal gene are disclosed. The compositions can be used as vaccines or carrier vehicles for delivering a desired protein to an individual. Also disclosed are methods for immunizing an individual and methods of delivering a desired gene product to an individual based upon administration of the compositions.
-
7.发明公开MICROBES HAVING AN ATTENUATING MUTATION COMPRISING A TRANSCRIPTION TERMINATOR 有权与转录漏失含STEAMING突变GERMS
公开(公告)号：EP1326960A2
公开(公告)日：2003-07-16
申请号：EP01979646.5
申请日：2001-10-11
Applicant: Washington University in St. Louis , Megan Health, Inc. , Curtiss, Roy III , Tinge, Steven A.
Inventor： CURTISS, Roy, III , TINGE, Steven, A.
CPC classification number: C12N15/74 , A61K39/0006 , A61K2039/522 , A61K2039/53 , Y02A50/407
Abstract: Compositions comprising a microbe having an attenuating mutation comprising a recombinant transcription terminator insertion in a chromosomal gene are disclosed. The compositions can be used as vaccines or carrier vehicles for delivering a desired protein to an individual. Also disclosed are methods for immunizing an individual and methods of delivering a desired gene product to an individual based upon administration of the compositions.
-
公开(公告)号：EP0832255A1
公开(公告)日：1998-04-01
申请号：EP96919292.0
申请日：1996-06-07
Applicant: WASHINGTON UNIVERSITY
Inventor： CURTISS, Roy, III , TINGE, Steven, A.
Abstract: Disclosed in an Environmentally Limited Viability System (ELVS) for microorganisms based on differences between permissive and non-permissive environments. Viability of the microorganisms are limited to a permissive environment by specifically expressing one or more essential genes only in the permissive environment, and/or expressing one or more lethal genes only in the non-permissive environment. Temporary viability in a non-permissive environment can be achieved by temporarily expressing one or more essential genes in a non-permissive environment, and/or temporarily delaying expression of one or more lethal genes in the non-permissive environment. Environmentally Limited Viabilty Systems are also disclosed involving coordinate expression of a combination of required genes and lethal genes. Microorganisms containing an Environmentally Limited Viability System are useful for release into permissive and non-permissive environments. Temperature regulated Environmentally Limited Viability Systems are particularly suited for delivery of expression products, such as antigens, using recombinant avirulent Salmonella by limiting their growth to the warmer environment inside the host, or by allowing growth for only a limited time in the host.
-
-
-
-
-
-
-
Search Results
8
records
(took 0.031 seconds)
