公开(公告)号：KR1020120125835A

公开(公告)日：2012-11-19

申请号：KR1020110043519

申请日：2011-05-09

Applicant: 대한민국(농촌진흥청장)

Inventor： 박동석 ,  김정구

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

CPC classification number: C12Q1/6844 ,  C12Q1/04 ,  C12R1/01

Abstract: PURPOSE: A primer set for detecting Pectobacterium wasabiae causing soft rot is provided to quickly and accurately detect Pectobacterium wasabiae and to prevent soft rot. CONSTITUTION: A primer set for detecting Pectobacterium wasabiae contains a primer with nucleic acid sequences of sequence numbers 1 and 2. A method for detecting Pectobacterium wasabiae comprises a step of amplifying DNA isolated from a plant sample and a step of identifying the PCR products. A composition for detecting Pectobacterium wasabiae contains the primer set. A PCR kit contains the primer set.

Abstract translation: 目的：提供一种用于检测引起软腐病的棘皮杆菌的引物组，用于快速准确地检测败血性贝氏杆菌并防止软腐。 构成：用于检测棘皮杆菌的引物组含有具有序列号1和2的核酸序列的引物。检测棘皮杆菌的方法包括扩增从植物样品分离的DNA的步骤和鉴定PCR产物的步骤。 用于检测棘皮杆菌的组合物含有引物组。 PCR试剂盒包含引物组。

公开(公告)号：KR1020120098563A

公开(公告)日：2012-09-05

申请号：KR1020120081367

申请日：2012-07-25

Applicant: 건국대학교 산학협력단 ,  대한민국(농촌진흥청장)

Inventor： 강린우 ,  안예진 ,  이병무 ,  김정구

IPC: C07D487/04 ,  A61K31/519 ,  A61P31/00 ,  A23K20/195

Abstract: PURPOSE: A novel antibiotic compound and an antibiotic composition containing the same are provided to suppress bacteria growth and to prevent or treat bacterial infection. CONSTITUTION: A novel antibiotic compound is denoted by chemical formula 7. The compound suppress peptidyl diformylase(PDF) activation. A pharmaceutical composition for preventing or treating infection by pathogen contains the compound of chemical formula 7 and pharmaceutically acceptable carrier. A feed composiiton for preventing or treating infection by pathogen contains the compound of chemical formula 7 and acceptable carriers. An agrochemical composition for preventing or treating infection by plant pathogen contains the compound and agrochemically acceptable carriers.

Abstract translation: 目的：提供一种新型抗生素化合物和含有该抗生素的抗生素组合物，以抑制细菌生长并预防或治疗细菌感染。 构成：化学式7表示新型抗生素化合物。化合物抑制肽基二甲酰基酶（PDF）激活。 用于预防或治疗病原体感染的药物组合物含有化学式7化合物和药学上可接受的载体。 用于预防或治疗病原体感染的饲料组合物含有化学式7化合物和可接受的载体。 用于预防或治疗植物病原体感染的农药组合物含有化合物和农业化学上可接受的载体。

公开(公告)号：KR1020100008715A

公开(公告)日：2010-01-26

申请号：KR1020080069318

申请日：2008-07-16

Applicant: 대한민국(농촌진흥청장)

Inventor： 이병무 ,  박영진 ,  김정구 ,  송은성 ,  조희정 ,  김상윤 ,  조재용

IPC: C12N15/31 ,  C12N15/10 ,  C12N15/09 ,  C12N1/20

Abstract: PURPOSE: A mutant strain which enables xanthan gum containing a mutant gene is provided to massively produce xanthan gum under the conventional technique and installation. CONSTITUTION: A mutant wxoD gene which is related to xanthan gum synthesis is derived from Xanthomonas oryzae strain. The Xanthomonas oryzae strain is Xanthomonas oryzae pv. Oryzae(KACC 10331) strain. The mutant gene wxoD is mutated by inserting the transposon. The mutant gene wxoD contains sequence of sequence number 1. A method for preparing the Xanthomonas sp.(deposit number: KACC91351) comprises: a step of adding transposon to the Xanthomonas sp.; a step of searching the mutant wxo gene using a specific primer; and a step of measuring biosynthesis rate of the xanthangum in the mutant strain and selecting.

Abstract translation: 目的：提供含有突变基因的含有黄原胶的突变菌株，以常规技术和设备大规模生产黄原胶。 构成：与黄原胶合成相关的突变型wxoD基因衍生自黄单胞菌属（Yanthomonas oryzae）菌株。 黄单胞菌属黄单胞菌属菌株是黄单胞菌属（Xanthomonas oryzae pv。 Oryzae（KACC 10331）菌株。 突变基因wxoD通过插入转座子而突变。 突变基因wxoD含有序列号1的序列。制备黄单胞菌属（保藏号：KACC91351）的方法包括：将转座子加入黄单胞菌属的步骤。 使用特异性引物搜索突变型wxo基因的步骤; 并测定突变菌株中黄原胶的生物合成速率并进行选择的步骤。

公开(公告)号：KR100842447B1

公开(公告)日：2008-07-01

申请号：KR1020060128550

申请日：2006-12-15

Applicant: 대한민국(농촌진흥청장)

Inventor： 이병무 ,  노태환 ,  김영태 ,  김정구 ,  조희정

IPC: C12N15/10 ,  C12Q1/68 ,  C12N15/11

Abstract: 본 발명은 서열번호 1의 염기서열을 포함하는 벼흰잎마름병원균의 레이스 판별용 유전자 마커(genetic marker) 및 벼흰잎마름병원균의 레이스의 판별방법에 관한 것으로 보다 상세하게는 서열번호 1의 염기서열을 포함하는 벼흰잎마름병원균의 레이스 판별용 유전자 마커 및 상기 프로브를 제한효소에 의해 절단된 벼흰잎마름병원균 게놈 DNA와 혼성화시키는 것을 특징으로 하는 벼흰잎마름병원균의 레이스의 판별방법에 관한 것이다. 본 발명의 벼흰잎마름병원균의 레이스의 판별방법은 벼흰잎마름병원균의 레이스를 효율적으로 판별할 수 있는 효과를 갖는다. 따라서 본 발명의 판별방법은 벼흰잎마름병원균의 레이스 판별의 목적으로 사용할 수 있다.
벼흰잎마름병, 벼흰잎마름병원균, RFLP, 레이스, 판별

公开(公告)号：KR1020040079052A

公开(公告)日：2004-09-14

申请号：KR1020030013954

申请日：2003-03-06

Applicant: 대한민국(농촌진흥청장)

Inventor： 박동석 ,  이병무 ,  한장호 ,  고승주 ,  강희완 ,  김정구 ,  이근표 ,  박영진

IPC: C12Q1/68

Abstract: PURPOSE: Primer sets for specific identification of a pathogen, Xanthomonas campestris pv. campestris, and a specific-identification method of the pathogen using the same primers are provided to rapidly and accurately identify Xanthomonas campestris pv. campestris. Therefore, the primer sets can be useful for prevention and control of Xanthomonas campestris pv. campestris. CONSTITUTION: The primer sets for specific identification of a pathogen, Xanthomonas campestris pv. campestris, amplify the total or a portion of 1st to 650th nucleotides in the nucleotide sequence of SEQ ID NO:1 of a gene hrpF, wherein the primer sets have the nucleotide sequences of SEQ ID NO:2 and SEQ ID NO:3. The specific-identification method of Xanthomonas campestris pv. campestris comprises the steps of: (A) extracting the total DNA from a plant sample; (B) PCR amplifying the extracted total DNA; and (C) verifying the amplification of the total or a portion of 1st to 650th nucleotides in the nucleotide sequence of SEQ ID NO:1 of a gene hrpF.

Abstract translation: 目的：用于特异性鉴定病原体的引物组，野油菜黄单胞菌（Xanthomonas campestris pv。） 提供了使用相同引物的病原体的特异性鉴定方法，以快速准确地鉴定野油菜黄单胞菌（Xanthomonas campestris pv。 油菜。 因此，引物组可用于预防和控制野油菜黄单胞菌（Xanthomonas campestris pv。 油菜。 构成：用于特异性鉴定病原体的引物组，野油菜黄单胞菌（Xanthomonas campestris pv。 菜籽油，扩增基因hrpF的SEQ ID NO：1的核苷酸序列中第1至第650位核苷酸的总数或部分，其中引物组具有SEQ ID NO：2和SEQ ID NO：3的核苷酸序列。 野油菜黄单胞菌的特异性鉴定方法 营养食品包括以下步骤：（A）从植物样品中提取总DNA; （B）PCR扩增提取的总DNA; 和（C）验证基因hrpF的SEQ ID NO：1的核苷酸序列中第1至第650位核苷酸的总数或部分的扩增。