公开(公告)号：KR100490980B1

公开(公告)日：2005-05-23

申请号：KR1020020075720

申请日：2002-11-30

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 박종현 ,  차상호 ,  송재영 ,  안동준 ,  최은진 ,  김종염 ,  권준헌

IPC: C12N15/86

Abstract: 본 발명은 각각 일본 뇌염 바이러스 유전자 prME 및 NS1이 삽입된 재조합 벡터 pSLIA-prME 및 pSLIA-NS1, 및 돼지 인터류킨(interleukin; IL)-2가 삽입된 재조합 벡터 pPIL-2, 이들의 일본 뇌염에 대한 DNA 백신으로서의 용도, 및 이들의 접종방법에 관한 것이다.

公开(公告)号：KR100442329B1

公开(公告)日：2004-08-02

申请号：KR1020020006191

申请日：2002-02-04

Applicant: 주식회사 엠아이텍 ,  대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 정인권 ,  안동준 ,  박찬승 ,  안성순 ,  송재영 ,  박종현 ,  차상호 ,  최은진 ,  권준헌

IPC: A61M5/20

Abstract: PURPOSE: An injector for livestock is provided to improve inoculation efficiency since a continuous inoculation is possible, inoculate precisely on an inoculation region of moving livestock and make maintenance easier due to its simple structure. CONSTITUTION: The injector for livestock comprises: an injector main body(2) which has a pressure handle(H) slidably mounted; a chemical feeding unit which injects a chemical by the operation of the pressure handle(H); and a display unit which is mounted at one side face of the chemical feeding unit and displays whether inoculation is carried out or not.

Abstract translation: 目的：提供牲畜注射器以提高接种效率，因为可以连续接种，由于结构简单，准确接种在移动家畜的接种区域并使维护更容易。 构成：家畜用注射器包括：注射器主体（2），其具有可滑动地安装的压力手柄（H） 通过压力手柄（H）的操作而注入化学品的化学供给单元; 以及显示单元，其安装在化学供给单元的一个侧面并显示是否进行接种。

公开(公告)号：KR1020040062218A

公开(公告)日：2004-07-07

申请号：KR1020020088561

申请日：2002-12-31

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 메디안디노스틱

Inventor： 최은진 ,  송재영 ,  박종현 ,  안동준 ,  차상호 ,  권준헌 ,  장병식 ,  박흥숙 ,  방선영

IPC: C12N5/18

CPC classification number: G01N33/56983 ,  C07K16/1081 ,  C07K2317/14 ,  G01N33/577 ,  G01N2333/183

Abstract: PURPOSE: A monoclonal antibody against classical swine fever virus (CSFV), a hybridoma cell line producing the same, and a method for detecting CSFV using the same are provided, thereby significantly reducing the time for detecting the classical swine fever virus (CSFV), and improving the accuracy of the detection. CONSTITUTION: The monoclonal antibody against classical swine fever virus (CSFV) is produced from the hybridoma cell line CSFV-LOM 1(KCLRF-BP-00069) or hybridoma cell line CSFV-LOM 2(KCLRF-BP-00070). A conjugate of peroxidase and monoclonal antibody against CSFV is provided. The method for detecting CSFV comprises the steps of: (1) diluting the monoclonal antibody against CSFV in a coating buffer solution, spotting the diluted solution on ELISA plate, and adsorbing it; (2) cleaning the ELISA plate to remove unadsorbed monoclonal antibody; (3) reacting a feces sample with the plate; (4) cleaning the plate to remove the feces sample unbound by the monoclonal antibody for coating; (5) reacting the monoclonal antibody for detecting with an enzyme conjugate; (6) cleaning the plate to remove unbound conjugate; and (7) adding a substrate into the plate and measuring the absorbance by the chromogenic reaction.

Abstract translation: 目的：提供针对经典猪瘟病毒（CSFV）的单克隆抗体，产生该抗体的杂交瘤细胞系和使用其的CSFV检测方法，从而大大减少了检测典型猪瘟病毒（CSFV）的时间， 并提高检测的准确性。 构成：从杂交瘤细胞系CSFV-LOM1（KCLRF-BP-00069）或杂交瘤细胞系CSFV-LOM2（KCLRF-BP-00070）产生针对经典猪瘟病毒（CSFV）的单克隆抗体。 提供过氧化物酶和针对CSFV的单克隆抗体的缀合物。 检测CSFV的方法包括以下步骤：（1）在涂布缓冲溶液中稀释针对CSFV的单克隆抗体，将稀释溶液点在ELISA板上，并吸附; （2）清洗ELISA板以去除未吸附的单克隆抗体; （3）将粪便样品与板反应; （4）清洗平板以除去单克隆抗体未结合的粪便样品进行涂层; （5）将用于检测的单克隆抗体与酶缀合物反应; （6）清洗板以除去未结合的结合物; 和（7）将底物加入板中并通过显色反应测量吸光度。

公开(公告)号：KR100261050B1

公开(公告)日：2000-07-01

申请号：KR1019980000443

申请日：1998-01-10

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 송재영 ,  차상호 ,  박종현 ,  현방훈 ,  안동준 ,  안수환

IPC: G01N33/53

Abstract: PURPOSE: Provided is a device for detecting antibody of Aujeszky's disease. And a method for diagnosing Aujeszky virus more rapidly and correctly using the same is also provided. CONSTITUTION: The device for detecting antibody of Aujeszky's disease is composed of; i) a nitrocellulose membrane (5), absorption pad (3), supporting plate (6), and soaking section (4). The method for detecting antibodies of Aujeszky virus is comprised of the following steps of: i) spreading antigens (1) of Aujeszky virus and anti-swine IgG (2) evenly on the nitrocellulose membrane (5); and ii) reacting the antigens (1) and IgG (2) with antibodies of Aujeszky virus in the soaking section (4), which shows different colors depending on the existence of antibodies.

Abstract translation: 目的：提供了一种用于检测Aujeszky病的抗体的装置。 还提供了使用该方法更快速和正确地诊断Aujeszky病毒的方法。 构成：检测Aujeszky病抗体的装置由以下组成： i）硝酸纤维素膜（5），吸收垫（3），支撑板（6）和浸泡部分（4）。 用于检测Aujeszky病毒抗体的方法包括以下步骤：i）将Aujeszky病毒和抗猪IgG（2）的抗原（1）均匀地铺展在硝酸纤维素膜（5）上; 和ii）在浸泡部分（4）中使抗原（1）和IgG（2）与Aujeszky病毒的抗体反应，其根据抗体的存在显示不同的颜色。

公开(公告)号：KR1020000019418A

公开(公告)日：2000-04-06

申请号：KR1019980037509

申请日：1998-09-11

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 현방훈 ,  송재영 ,  안동준 ,  박종현 ,  차상호 ,  안수환

IPC: C12N15/63 ,  C12N15/86

CPC classification number: C12N15/86 ,  A61K39/12 ,  C12N7/00

Abstract: PURPOSE: Recombinant porcine Aujesky's disease virus provides safe vector vaccine against porcine diseases and can be used to produce effective vaccine against other diseases if antigen of other virus is introduced in it. CONSTITUTION: Recombinant porcine Aujesky's disease virus is useful to prepared a vaccine vector. The vector is constructed by; deletion of K gene; insertion of IL-2 gene; deletion of gI gene; insertion of beta-galactosidase gene as a maker gene; and insertion of foreign gene coding antigen for virus pathogen. Thereby, immunity is improved.

Abstract translation: 目的：重组猪Aujesky病病毒提供安全的针对猪疾病的载体疫苗，如果其中引入其他病毒的抗原，可用于产生针对其他疾病的有效疫苗。 构成：重组猪Aujesky病病毒可用于制备疫苗载体。 矢量由...构建; 缺失K基因; 插入IL-2基因; 缺失gI基因; 插入β-半乳糖苷酶基因作为制造者基因; 并插入外源基因编码抗原用于病毒病原体。 从而，免疫力得到改善。

公开(公告)号：KR1020000003768A

公开(公告)日：2000-01-25

申请号：KR1019980025040

申请日：1998-06-29

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 현방훈 ,  송재영 ,  안동준 ,  박종현 ,  차상호 ,  안수환

IPC: C12N15/86 ,  C12N15/33

CPC classification number: C12N15/861 ,  C07K14/005

Abstract: PURPOSE: Provided are a fluorescent vector, which is used for the production of vaccine of porcine Aujesky's disease and a method for selecting recombination virus using the same. CONSTITUTION: A fluorescent vector pHAVE3 is prepared by amplifying pVIII gene corresponding to 5' region of E3 gene of human Adenovirus type II, a partial sequence(27100-28811bp) having 1,712base, before open reading frame of E3 gene being 19kd, a partial sequence(30,851-32,760bp) having 1,910base, after E3-2 polyadenylation signal corresponding to 3' region of E3 gene; cloning them into SpeI of plasmid pGEMT; and inserting BamH I restriction site

Abstract translation: 目的：提供一种荧光载体，其用于生产猪Aujesky病疫苗，以及使用该载体选择重组病毒的方法。 构成：荧光载体pHAVE3通过扩增对应于人腺病毒II型E3基因的5'区域的pVIII基因，其具有1,712碱基的部分序列（27100-28811bp），E3基因的开放阅读框为19kd，部分 序列（30,851-32760bp）具有1,910个碱基，E3-2多聚腺苷酸化信号对应于E3基因的3'区域; 将其克隆入SpeI质粒pGEMT; 并插入BamH I限制位点