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    곤충글라이코자미노글라이칸의 신규 용도 및 제조방법
    122.
    发明公开
    곤충글라이코자미노글라이칸의 신규 용도 및 제조방법 无效
    从胰岛素衍生的谷氨酰胺糖蛋白的新用途和糖蛋白酶体的制备方法

    公开(公告)号：KR1020110013074A

    公开(公告)日：2011-02-09

    申请号：KR1020090071072

    申请日：2009-07-31

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 안미영 ,  황재삼 ,  윤형주 ,  윤은영

    IPC: A61K35/64 ,  A61K31/7028 ,  A61K8/98 ,  A61K8/60

    CPC classification number: A61K8/60 ,  A61K8/987 ,  A61K31/7028 ,  A61K35/63

    Abstract: PURPOSE: An insect glycosaminoglycan and a method for preparing the same are provided to ensure anti-inflammation, anti-diabetes and anticancer and to prevent and/or treat diseases caused by functional disorder of vasodilation. CONSTITUTION: A composition for anti-inflammation contains insect glycosaminoglycan. The insect glycosaminoglycan is cicada Paecilomyces japonica glycosaminoglycan, glycosaminoglycan having repetitive ΔUA-2S-[1-4]GlcN structure or Velarifictorus asperses glycosaminoglycan. The composition is a pharmaceutical composition or cosmetic composition. A composition for anti-diabetes or anticancer contains the insect glycosaminoglycan as an active ingredient.

    Abstract translation: 目的：提供昆虫糖胺聚糖及其制备方法以确保抗炎，抗糖尿病和抗癌，并预防和/或治疗由血管舒张功能障碍引起的疾病。 构成：抗炎组合物含有昆虫糖胺聚糖。 昆虫糖胺聚糖是蝉拟青霉糖糖胺聚糖，具有重复ΔUA-2S- [1-4] GlcN结构的糖胺聚糖或Velarifictorus asperses糖胺聚糖。 该组合物是药物组合物或化妆品组合物。 用于抗糖尿病或抗癌的组合物含有昆虫糖胺聚糖作为活性成分。

    애기뿔소똥구리로부터 분리된 항진균 활성을 가지는 코프리신 펩타이드 및 그의 용도
    123.
    发明公开
    애기뿔소똥구리로부터 분리된 항진균 활성을 가지는 코프리신 펩타이드 및 그의 용도 有权
    从三叶草中提取的抗真菌肽及其使用

    公开(公告)号：KR1020100108793A

    公开(公告)日：2010-10-08

    申请号：KR1020090027004

    申请日：2009-03-30

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 황재삼 ,  윤은영 ,  김성렬 ,  남성희 ,  안미영 ,  구태원 ,  강석우 ,  이동건 ,  이준영

    IPC: C07K14/435 ,  A61K38/17

    Abstract: PURPOSE: A coprisin peptide with antifungal activity, isolated from Copris tripartitus is provided to apply to antibiotics, food preservative, cosmetic product preservative, and medicine preservative without cytotoxicity. CONSTITUTION: A peptide isolated from Copris tripartitus has an antifungal activity and is denoted by sequence number 1. A coprisin is the peptide isolated from Copris tripartitus. The peptide comprises 43 amino acids. An antifungal pharmaceutical composition contains the peptide of sequence number 1 as an active ingredient.

    Abstract translation: 目的：从Copris triartitus分离出具有抗真菌活性的共立克斯肽，适用于抗生素，食品防腐剂，化妆品防腐剂和无细胞毒性的药物防腐剂。 构成：从Copris triartitus分离的肽具有抗真菌活性，并由序列号1表示。共感染蛋白是从Copris triartitus分离的肽。 肽包含43个氨基酸。 抗真菌药物组合物含有序列号1的肽作为活性成分。

    곤충세포주에 시알산 전이효소 유전자 및 갈락토오스전이효소 유전자가 형질전환된 당사슬 부가기능이 향상된형질전환 곤충세포주 및 그 제조 방법
    124.
    发明公开
    곤충세포주에 시알산 전이효소 유전자 및 갈락토오스전이효소 유전자가 형질전환된 당사슬 부가기능이 향상된형질전환 곤충세포주 및 그 제조 방법 无效
    在表达稳定的人β细胞的INSEC细胞中产生的糖蛋白的合成化; 1,4-糖苷突变蛋白酶和大鼠α2,6-肌醇六磷酸酶及其方法

    公开(公告)号：KR1020070060439A

    公开(公告)日：2007-06-13

    申请号：KR1020050119819

    申请日：2005-12-08

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 윤은영 ,  구태원 ,  황재삼 ,  최광호 ,  강석우 ,  권오유

    IPC: C12N15/63 ,  C12N15/10

    Abstract: A transformed insect cell containing human beta-1,4-galactosyltransferase and rat alpha-2,6-sialyltransferase and having an enhanced sugar chain addition function and a production method of glycoproteins from the same transformed insect cells are provided to improve production stability and economic efficiency of useful sugar proteins derived from human by using inexpensive insect cells. The transformed insect cell containing human beta-1,4-galactosyltransferase and rat alpha-2,6-sialyltransferase and having the enhanced sugar chain addition function is produced by transforming an insect cell line Sf9 with a recombinant vector containing the human beta-1,4-galactosyltransferase gene and rat alpha-2,6-sialyltransferase gene. The glycoproteins which are added sialic acid or sialic acid and galactose are produced by culturing the transformed insect cell.

    Abstract translation: 提供含有人β-1,4-半乳糖基转移酶和大鼠α-2,6-唾液酸转移酶的转化的昆虫细胞，并且具有增强的糖链添加功能和来自相同转化的昆虫细胞的糖蛋白的制备方法以提高生产稳定性和经济性 通过使用便宜的昆虫细胞从人类衍生的有用糖蛋白的效率。 通过用包含人β-1的重组载体转化昆虫细胞系Sf9来产生含有人β-1,4-半乳糖基转移酶和大鼠α-2,6-唾液酸转移酶并具有增强的糖链加成功能的转化的昆虫细胞， 4-半乳糖基转移酶基因和大鼠α-2,6-唾液酸转移酶基因。 通过培养转化的昆虫细胞产生加入唾液酸或唾液酸和半乳糖的糖蛋白。

    곤충 세포주 형질전환용 발현벡터 및 이를 함유한 항세균단백질 생산용 형질전환체
    125.
    发明公开
    곤충 세포주 형질전환용 발현벡터 및 이를 함유한 항세균단백질 생산용 형질전환체 有权
    用于转化插入细胞系的表达载体及其生产抗生素蛋白的转化体

    公开(公告)号：KR1020010074351A

    公开(公告)日：2001-08-04

    申请号：KR1020000003378

    申请日：2000-01-25

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 김근영 ,  진병래 ,  강석우 ,  윤은영 ,  황재삼 ,  조은숙

    IPC: C12N15/63

    CPC classification number: C12N15/866 ,  C12N2511/00

    Abstract: PURPOSE: An expression vector for transforming an insect cell line is provided which has high expression efficiency and is thus used in mass-producing a foreign gene product. And an insect cell line transformant is also provided, which introduces the vector and produces antibacterial protein, Nuecin. CONSTITUTION: The expression vector pAcIE1-Neo-hsp consists of Baculovirus AcNPV IE1 promoter-neomycin resistant gene-Drosophila heat shock protein promoter-foreign gene inserting site(restriction enzyme EcoRV)-SV40 poly(A)-IE1 structural gene. The expression vector pAcIE1-Neo-hsp is constructed by: inserting neomycin resistant gene under the control of IE1 gene promoter of insect Baculovirus AcNPV; introducing a strong Drosophila heat shock protein promoter; placing a foreign gene inserting site, EcoRV site under the control of the promoter; and inserting animal virus, SV40 poly(A) site for the termination of transcription. The expression vector pAcIE1-Neo-hsp-Nuecin is constructed by inserting Nuecin cDNA gene into foreign gene inserting site, EcoRV site of pAcIE1-Neo-hsp.

    Abstract translation: 目的：提供用于转化昆虫细胞系的表达载体，其具有高表达效率，因此用于批量生产外源基因产物。 还提供了一种昆虫细胞系转化体，其引入载体并产生抗菌蛋白质Nuecin。 构成：表达载体pAcIE1-Neo-hsp由杆状病毒AcNPV IE1启动子 - 新霉素抗性基因 - 果蝇热休克蛋白启动子 - 外源基因插入位点（限制酶EcoRV）-SV40聚（A）-IE1结构基因组成。 构建表达载体pAcIE1-Neo-hsp：将新霉素抗性基因插入昆虫杆状病毒AcNPV的IE1基因启动子控制下; 引入强果蝇热休克蛋白启动子; 将外源基因插入位点，EcoRV位点置于启动子的控制下; 并插入动物病毒，SV40 poly（A）位点终止转录。 通过将Nuecin cDNA基因插入外源基因插入位点，pAcIE1-Neo-hsp的EcoRV位点构建表达载体pAcIE1-Neo-hsp-Nuecin。

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