부탄올 고생산 균주를 이용한 고생산성 부탄올 생산 방법
    121.
    发明授权
    부탄올 고생산 균주를 이용한 고생산성 부탄올 생산 방법 失效
    使用高丁醇滴度的微生物生产高产丁醇的方法

    公开(公告)号:KR101149882B1

    公开(公告)日:2012-05-25

    申请号:KR1020100003900

    申请日:2010-01-15

    Abstract: PURPOSE: A method for producing butnaol of high productivity using a strain is provided to be used in industrial technology. CONSTITUTION: A mutant Clostridium beijerinckii CBE-2-68 which produces butanol with high yield is deposited in the deposit number KCTC11606BP. A method for producing a large amount of butanol comprises: a step of culturing the Clostridium beijerinckii CBE-2-68 strain; a step of centrifuging the culture liquid to collect supernatant; and a step of purifying butanol.

    효소 활성이 증가된 신규한 리파제 변이체
    122.
    发明公开
    효소 활성이 증가된 신규한 리파제 변이체 有权
    具有改善脂肪酶活性的脂肪变体

    公开(公告)号:KR1020110060384A

    公开(公告)日:2011-06-08

    申请号:KR1020090116957

    申请日:2009-11-30

    Abstract: PURPOSE: A Candida Antarctica-derived lipase B(CalB) protein variant is provided to increase enzyme activity and to be used in the industry. CONSTITUTION: A CalB protein variant is selected from a protein of amino acid sequences 2-8 in which 274-289th amino acid is partially substituted in Candida Antarctica-derived lipase B(CalB) protein. A gene encoding CalB protein variant sequence is denoted by sequence number selected from sequence numbers 10-16. A recombinant vector containing the gene is prepared by inserting the gen into pK7 vector. A transformed cell which produces CalB protein variant is obtained by transforming a host cell with the recombinant vector. The host cells are Pichia pastoris.

    Abstract translation: 目的:提供来自南极假丝酵母的脂肪酶B(CalB)蛋白质变体以增加酶活性并用于该行业。 构成:CalB蛋白质变体选自氨基酸序列2-8的蛋白质,其中274-289位氨基酸被部分取代在南极假单胞菌来源的脂肪酶B(CalB)蛋白质中。 编码CalB蛋白变体序列的基因由选自序列号10-16的序列号表示。 通过将gen插入pK7载体中制备含有该基因的重组载体。 通过用重组载体转化宿主细胞获得产生CalB蛋白质变体的转化细胞。 宿主细胞是巴斯德毕赤酵母。

    대장균의 외막단백질 OmpW를 이용한 목적단백질의 표면발현 방법
    123.
    发明公开
    대장균의 외막단백질 OmpW를 이용한 목적단백질의 표면발현 방법 失效
    方法使用大肠杆菌OMPW对目标蛋白进行细胞表面显示

    公开(公告)号:KR1020100072848A

    公开(公告)日:2010-07-01

    申请号:KR1020080131375

    申请日:2008-12-22

    Abstract: PURPOSE: A surface expression vector of a foreign protein containing C-terminal deleted OmpW is provided to use in protein array, antibody manufacture, bioconversion, and foreign protein improvement. CONSTITUTION: A surface expression vector of a foreign protein contains C-terminal deleted OmpW and foreign protein gene. In OmpW, C-terminal which is downstream of 139th amino acid and downstream of 191th amino acid is deleted. A transformant is prepared by transducing the surface expression vector of a foreign protein into a host cell. The host cell is E.coli. A method for preparing antibody using the cell on which foreign protein is expressed comprises: a step of administering the cell to the host cell to induce immune response; and a step of collecting produced antibody in an individual.

    Abstract translation: 目的:提供含有C-末端缺失OmpW的外源蛋白的表面表达载体,用于蛋白质阵列,抗体制备,生物转化和外源蛋白质改良。 构成:外来蛋白质的表面表达载体含有C末端缺失的OmpW和外来蛋白质基因。 在OmpW中,缺失了第139位氨基酸下游和第191位氨基酸下游的C末端。 通过将外源蛋白质的表面表达载体转导入宿主细胞来制备转化体。 宿主细胞是大肠杆菌。 使用表达外源蛋白质的细胞制备抗体的方法包括:向宿主细胞施用细胞以诱导免疫应答的步骤; 以及在个体中收集产生的抗体的步骤。

    n―부탄올의 제조 방법
    125.
    发明公开
    n―부탄올의 제조 방법 失效
    制备N-丁醇的方法

    公开(公告)号:KR1020090049656A

    公开(公告)日:2009-05-19

    申请号:KR1020070115831

    申请日:2007-11-14

    Abstract: 본 발명은, 구리계 촉매를 사용하여 물을 사용하지 않는 반응 조건 하에서, n-부탄산을 단독으로 직접 기상 수소화시키거나 또는 무수 n-부탄산이나 n-부탄산의 n-부틸에스테르와 같은 부탄산 유도체를 함유한 n-부탄산을 직접 기상 수소화하는 공정에 있어서 부반응의 억제 하에 높은 선택성 및 높은 공간 수율로 n-부탄올을 제조할 수 있는 수소화 방법을 제공하는 것이다. 구체적으로, 본 발명은, 환원된 구리계 촉매 상에서 n-부탄산을 수소에 의하여 직접 기상 환원시키는 것을 포함하는, n-부탄올의 제조 방법으로서, 상기 환원형 구리계 촉매가, 실리카, 알루미나, 티타니아 및 산화아연으로 이루어진 군에서 선택되는 하나 이상의 희석제와 산화구리 성분의 복합 산화물을 환원시켜 수득된 구리계 촉매이거나, 또는 상기 촉매에 코발트, 아연, 망간, 루테늄, 레늄, 팔라듐, 백금, 은, 텔루륨, 셀레륨, 마그네슘 및 칼슘으로 이루어진 군에서 선택되는 하나 이상의 개량성분을 추가로 포함한 촉매로서, 산화구리 성분의 함량이 40~95wt%이고 산화구리 입자크기가 50nm 이하가 되도록 제조된 촉매이다.
    n-부탄산, n-부탄올, 바이오부탄올, 환원형 구리계 촉매

    천연 페놀계 에폭시 수지의 제조방법 및 그 용도
    126.
    发明公开
    천연 페놀계 에폭시 수지의 제조방법 및 그 용도 失效
    天然酚羟基环氧树脂的制备方法及其应用

    公开(公告)号:KR1020060126084A

    公开(公告)日:2006-12-07

    申请号:KR1020050047709

    申请日:2005-06-03

    Abstract: A method for preparing a natural phenolic epoxy resin is provided to obtain a phenolic epoxy resin useful as a solvent-free coating agent having excellent water resistance, acid resistance, alkali resistance and stability to an organic solvent. The method for preparing a natural phenolic epoxy resin comprises the steps of: forming an unsaturated double bond-containing phenolic polymer derived from plants; reacting an organic acid, hydrolase and hydrogen peroxide to form a peroxidized organic acid; and inserting oxygen atom into the unsaturated double bond of the plant-derived phenolic polymer by using the peroxidized organic acid. Particularly, the peroxidized organic acid is obtained by dissolving the phenolic polymer and the organic acid into an organic solvent, and introducing hydrolase and hydrogen peroxide thereto so that hydrogen peroxide is decomposed by hydrolase to provide oxygen, which, in turn, is bound to the organic acid.

    Abstract translation: 提供一种制备天然酚醛环氧树脂的方法,以获得可用作无机涂料的酚醛环氧树脂,其具有优异的耐水性,耐酸性,耐碱性和对有机溶剂的稳定性。 制备天然酚醛环氧树脂的方法包括以下步骤:形成由植物衍生的含不饱和双键的酚类聚合物; 使有机酸,水解酶和过氧化氢反应形成过氧化的有机酸; 并通过使用过氧化的有机酸将氧原子插入到植物来源的酚类聚合物的不饱和双键中。 特别地,过氧化有机酸是通过将酚类聚合物和有机酸溶解在有机溶剂中,并且向其中引入水解酶和过氧化氢以使过氧化氢通过水解酶分解以提供氧而获得的,其又结合到 有机酸。

    수율을 개선한 고지의 부상부유 탈묵방법
    127.
    发明授权
    수율을 개선한 고지의 부상부유 탈묵방법 有权
    수율을개선한고지의부상부유탈묵방법

    公开(公告)号:KR100644267B1

    公开(公告)日:2006-11-10

    申请号:KR1020050071606

    申请日:2005-08-05

    Abstract: A flotation de-inking method of waste paper with an improved yield is provided to perform the enzyme treatment of the first flotation reject powder. A hydrophobic material is concentrated after the floating process. The concentrated flotation reject is separated and discharged. One or more enzymes selected from a group consisting of esterase, lipase or cutinase are injected into the entire waste paper in a Froth-Flotation Cell and the only adsorbed material is selectively modified into hydrophilic material. The addition amount of the enzyme is 0.02-2 weight % relating to the dry weight of the entire waste paper. The hydrophobic material is the functional additive or cohesive foreign substance.

    Abstract translation: 提供具有提高产率的废纸的浮选脱墨方法以对第一浮选废料粉进行酶处理。 漂浮过程后疏水材料被浓缩。 浓缩的浮选废料被分离并排出。 将一种或多种选自酯酶,脂肪酶或角质酶的酶注入到泡沫浮选池中的整个废纸中,并将唯一吸附的材料选择性地修饰成亲水材料。 酶的添加量相对于整个废纸的干重为0.02-2重量%。 疏水性材料是功能性添加剂或粘性外来物质。

    고농도 폐수로부터 수소가스 및 메탄가스의 생산방법
    129.
    发明授权
    고농도 폐수로부터 수소가스 및 메탄가스의 생산방법 失效
    고농도폐수从수가스및메탄가스의생산방

    公开(公告)号:KR100461759B1

    公开(公告)日:2004-12-14

    申请号:KR1020020041691

    申请日:2002-07-16

    Abstract: PURPOSE: Provided is a method for generating hydrogen gas and methane gas from highly-concentrated wastewater by continuously or intermittently introducing digested granular sludge into wastewater. CONSTITUTION: The method comprises the steps of (a) introducing water-soluble granulation accelerator into sludge in a granular sludge generation tank to produce granular sludge, (b) introducing the granular sludge into an acid generation tank, thereby generating hydrogen gas in the acid generation tank by decomposing organic material using anaerobic microorganisms, and (c) introducing decomposed organic acid and granular sludge into a methane generation tank, and (d) generating methane gas in the methane generation tank by interaction of microorganisms in sludge.

    Abstract translation: 目的:提供一种通过连续或间歇地将消化的颗粒污泥引入废水而从高浓度废水产生氢气和甲烷气体的方法。 本发明的方法包括以下步骤:(a)将水溶性造粒促进剂引入颗粒污泥产生槽中的污泥中以产生颗粒污泥;(b)将颗粒污泥引入酸产生槽中,由此在酸中产生氢气 (c)将分解的有机酸和颗粒污泥引入甲烷发生罐中,以及(d)通过污泥中的微生物的相互作用在甲烷发生罐中产生甲烷气体。

    제지공정분석기
    130.
    发明授权

    公开(公告)号:KR100455563B1

    公开(公告)日:2004-11-12

    申请号:KR1020010040973

    申请日:2001-07-09

    Abstract: PURPOSE: Provided is a retention and drainage analyzer-handsheet former which can estimate synthetically holding degree, dehydrating ability, uniformity and strength of paper changing according to various sheet-forming conditions, and operate effectively a practical sheet-making process. CONSTITUTION: The retention and drainage analyzer-handsheet former is comprised of: (a paper-material composing part) a paper-material composing tank(10) capable of controlling the height according to quantity of the paper-material; plural impellers(14) which are installed in the paper-material composing tank(10), inducing turbulent flow in all directions; an automatic additive inputting device(13) putting various additives into the paper-material composing tank(10); (a forming part) a paper forming tank(20); (a white water collecting part) a white water tank(30); (a pressure reducing inlet) a vacuum tank(40); and a computer(50).

    Abstract translation: 用途:提供一种保留和排水分析器 - 手抄纸成形器,其可根据各种成形条件合成地估计纸张的保持程度,脱水能力,均匀性和强度变化,并且有效地操作实际的抄纸过程。 组成:保留和排水分析器 - 手抄纸成形器包括:(纸材料组成部分),能够根据纸材料的量控制高度的纸材料组成箱(10) 安装在纸质材料合成箱(10)中的多个叶轮(14),引起所有方向的湍流; 自动添加剂输入装置(13),其将各种添加剂放入纸质材料合成箱(10)中; (成形部分)纸张成形槽(20); (白水收集部分)白水罐(30); (减压入口),真空箱(40); 和一台计算机(50)。

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