公开(公告)号：WO1995000650A1

公开(公告)日：1995-01-05

申请号：PCT/US1994005626

申请日：1994-05-18

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ADAMS, Craig, W. ,  PANG, Patty, P., Y. ,  BELEI, C., Marina

IPC: C12N15/55

CPC classification number: C07K16/40 ,  C12N9/22 ,  C12Q1/44 ,  G01N2333/922

Abstract: The gene for Streptococcus pyogenes DNase B has been cloned and vectors incorporating the cloned DNA have been used to transform Escherichia coli, allowing production of the DNase in E. coli. The enzyme can be produced with a leader peptide at its amino terminus. Method for the purification of naturally occurring S. pyogenes DNase B enzyme is also provided. The DNase B enzyme produced, either by purification of naturally occurring enzyme or by recombinant DNA techniques, can be used to generate antibodies and can also be used in immunochemical assays to detect the presence of anti-DNase B antibodies in serum as a marker of infection by S. pyogenes.

Abstract translation: 已经克隆了化脓性链球菌DNase B的基因，并且使用掺入克隆DNA的载体转化大肠杆菌，从而在大肠杆菌中产生DNA酶。 该酶可以在其氨基末端用前导肽产生。 还提供了纯化天然存在的化脓性链球菌DNA酶B酶的方法。 通过纯化天然存在的酶或通过重组DNA技术产生的DNA酶B酶可用于产生抗体，也可用于免疫化学测定以检测血清中抗DNase B抗体作为感染标志物的存在 化脓性链球菌。

公开(公告)号：WO1994029024A1

公开(公告)日：1994-12-22

申请号：PCT/US1994004516

申请日：1994-04-25

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  GLENDAY, Ronald, C. ,  GOODALE, David, L. ,  MACK, Steven, D.

IPC: B01L03/00

CPC classification number: B01L3/5085 ,  G01N2035/0451

Abstract: A sample segment (10) including a body and wells (14) formed therein, selected wells (14b, 14f) including a flat exterior surface (56) surrounding a tapered end (64). The sample segment (10) may retain selected reagents (80), and a sealing cover (90) is held by ribs (74, 76), stretched and pressed against raised bosses (70) formed around the well openings (44) to provide a sure seal. The bosses (70) are discontinuous between adjacent wells (14). Selected wells may contain a free mixing element (82) for enhanced mixing. The segment (10) is uniquely adapted for automated handling and processing.

Abstract translation: 包括主体的样品段（10）和形成在其中的孔（14），包括围绕渐缩端（64）的平坦外表面（56）的选定孔（14b，14f）。 样品段（10）可以保留所选择的试剂（80），并且密封盖（90）由肋（74,76）保持，被拉伸并压靠在围绕井口（44）形成的凸起凸起（70）上，以提供 一个肯定的印章。 凸台（70）在相邻的孔（14）之间是不连续的。 所选择的孔可以包含用于增强混合的游离混合元件（82）。 该段（10）独特地适用于自动处理和处理。

公开(公告)号：WO1994007606A1

公开(公告)日：1994-04-14

申请号：PCT/US1993009127

申请日：1993-09-27

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  CHERN, Cory ,  PENHASI, Harry, A.

IPC: B04B07/02

CPC classification number: B04B7/02 ,  B04B5/0414 ,  B04B2007/025 ,  F16B2200/403 ,  Y10T16/44

Abstract: A centrifuge rotor lid (44) assembly which includes a handle (64) which allows convenient handling of a centrifuge rotor (30) and provides a means of releasing the lid (44) from the rotor (30) after centrifugation. In the described embodiment, the lid (44) is provided with a lid knob (48) and a spindle knob (50) in a coaxial sliding arrangement. The lid knob (48) is used to tighten the lid (44) on the rotor body (30) and the spindle knob (50) is used to tighten the rotor (30) to the centrifuge drive spindle (36). The upper end of the inner knob (50) is provided with a handle (64) which extends perpendicular to the axis of the knob. This handle (64) is used for lifting the rotor (30) after the spindle knob (50) has been unscrewed from the spindle (36) and for unscrewing the lid knob (48). The lower end of the spindle knob (50) has a pin (66) which cooperatively engages the lower end of the lid knob (48) when the spindle knob (50) is lifted from the rotor (30) and is turned in a direction to unscrew the lid knob (48). The handle provides the necessary mechanical advantage for a person to exert sufficient torque to unscrew the lid knob (48) without the use of external tools.

Abstract translation: 一种离心机转子盖（44）组件，其包括手柄（64），其允许方便地处理离心机转子（30）并提供在离心后从转子（30）释放盖子（44）的装置。 在所描述的实施例中，盖（44）设置有同轴滑动装置的盖把手（48）和主轴旋钮（50）。 盖旋钮（48）用于拧紧转子主体（30）上的盖（44），并且主轴旋钮（50）用于将转子（30）拧紧到离心机驱动主轴（36）。 内部旋钮（50）的上端设置有垂直于旋钮的轴线延伸的手柄（64）。 在主轴旋钮（50）已经从主轴（36）旋下并用于旋开盖子旋钮（48）之后，该把手（64）用于提升转子（30）。 主轴旋钮（50）的下端具有销（66），当主轴旋钮（50）从转子（30）提升时，该销（66）与盖旋钮（48）的下端协作地接合，并且沿着方向 拧下盖子旋钮（48）。 手柄提供了必要的机械优势，使人能够施加足够的扭矩来旋转盖旋钮（48）而不使用外部工具。

公开(公告)号：WO1994006745A1

公开(公告)日：1994-03-31

申请号：PCT/US1993007365

申请日：1993-08-05

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  CHENG, Anthony, K. ,  DOBASHI, Thomas

IPC: C07C55/00

CPC classification number: G01N33/9473 ,  C07C51/09 ,  C07C51/347 ,  C07C51/38 ,  C07C69/34 ,  C07C55/00 ,  C07C55/02

Abstract: A method for making a valproic acid derivative comprising a functionalized spacer arm attached to a (delta) carbon atom of a valproic acid molecule is disclosed. The method proceeds by attaching a spacer arm joined to an inorganic moiety to a valproic acid precursor to make an alkylated compound, derivatizing the alkylated compound in a liquid medium to make the valproic acid derivative, and then separating the valproic acid derivative from the liquid medium. The valproic acid derivative can be used to make an immunoreactive valproic acid conjugate.

Abstract translation: 公开了一种制备丙戊酸衍生物的方法，其包含连接于丙戊酸分子的（delta）碳原子的官能化间隔臂。 该方法通过将连接到无机部分的间隔臂连接到丙戊酸前体以制备烷基化化合物，在液体培养基中衍生化烷基化化合物以制备丙戊酸衍生物，然后将丙戊酸衍生物从液体培养基中分离 。 丙戊酸衍生物可用于制备免疫反应性丙戊酸缀合物。

公开(公告)号：WO1994002853A1

公开(公告)日：1994-02-03

申请号：PCT/US1993003724

申请日：1993-04-21

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  KEARNS, Elizabeth, K. ,  OH, Chan, S.

IPC: G01N33/557

CPC classification number: G01N33/557 ,  G01N21/272

Abstract: An initial rate photometric immunoassay method useful for detecting and quantifying analytes in various physiological fluids is disclosed. The method is carried out by combining in a liquid medium an undiluted sample of analyte-containing physiological fluid, such as serum, and an excess of an anti-analyte antibody. A substantially constant initial rate of increase of the liquid medium'sturbidity, due to the resulting immunoprecipitation reaction, is measured in real time and compared to a calibration curve prepared from known analyte concentrations to detect and quantify the analyte. Detection and quantification of numerous analytes including haptens, drugs and proteins, in a wide variety of physiological fluids can be rapidly carried out by the present method.

Abstract translation: 公开了用于检测和定量各种生理液体中的分析物的初始速率光度测定方法。 该方法通过在液体培养基中结合未稀释的含分析物的生理液体样品（例如血清）和过量的抗分析物抗体来进行。 实时测量由于所得的免疫沉淀反应引起的液体介质的浊度的基本恒定的初始增加速率，并与从已知分析物浓度制备的校准曲线进行比较，以检测和定量分析物。 通过本发明的方法可以快速地检测和定量多种生理液体中的许多分析物，包括半抗原，药物和蛋白质。

公开(公告)号：WO1993022329A1

公开(公告)日：1993-11-11

申请号：PCT/US1993003386

申请日：1993-04-09

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  REDDY, Parameswara, Meda ,  HANNA, Naeem, Botros

IPC: C07H21/00

CPC classification number: C07H21/00

Abstract: Disclosed are reagents of methods for cleaving and deprotecting insolubilized and protected synthetic oligonucleotides. In a particularly preferred embodiment, the reagent comprises methylamine and t-butylamine.

Abstract translation: 公开了用于裂解和去保护不溶性和保护的合成寡核苷酸的方法的试剂。 在特别优选的实施方案中，试剂包括甲胺和叔丁胺。

公开(公告)号：WO1993010258A1

公开(公告)日：1993-05-27

申请号：PCT/US1992009873

申请日：1992-11-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  HSIEH, You-Zung ,  CHEN, Fu-Tai, A. ,  STERNBERG, James, C. ,  KLIEN, Gerald ,  LIU, Cheng-Ming

IPC: C12Q01/00

CPC classification number: C12Q1/007 ,  G01N27/44747

Abstract: Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ('CZE') techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.

公开(公告)号：WO1993008675A1

公开(公告)日：1993-04-29

申请号：PCT/US1992009104

申请日：1992-10-21

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  GLEN, Stefan, J. ,  CARSTENS, Bradley, D.

IPC: B04B05/04

CPC classification number: B04B5/0414 ,  B04B2007/025

Abstract: A hybrid composite sample holder (14) utilized to improve overall strength-to-weight of a centrifuge rotor. The holder (14) comprises a body having a metal neck (62) and a fiber composite base (60) portion integrally attached.

Abstract translation: 用于提高离心机转子整体强度重量的混合复合样品架（14）。 保持器（14）包括具有金属颈部（62）和整体连接的纤维复合基底（60）部分的主体。

公开(公告)号：WO1993007482A1

公开(公告)日：1993-04-15

申请号：PCT/US1992008589

申请日：1992-10-08

Applicant: BECKMAN INSTRUMENTS, INC. ,  PENTONEY, Stephen, L., Jr.

Inventor： BECKMAN INSTRUMENTS, INC.

IPC: G01N30/74

CPC classification number: G01B11/272 ,  G01N27/44721 ,  G01N30/6082 ,  G01N30/74 ,  G01N2021/0346 ,  G01N21/05

Abstract: An apparatus and method of aligning a capillary (10) with respect to a radiation source (26). More particularly, the capillary is aligned with a laser beam (29) for laser induced fluorescence detection. The scatter or transmitted light pattern of the laser beam (29) with respect to the capillary (10) tube is utilized to determine optimum alignment. Photosensors (97, 99) may be implemented to detect the light pattern which represents optimum alignment. For dynamic alignment during electrophoresis, the photosensors provide feedback to a controller (124) which controls a positioning mechanism (122) with a plano-convex lens (120) for alignment of the capillary (10).

公开(公告)号：WO1993005156A1

公开(公告)日：1993-03-18

申请号：PCT/US1992006398

申请日：1992-07-31

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ADAMS, Craig, W. ,  WANG, Eva, Y.

IPC: C12N15/31

CPC classification number: C07K14/315

Abstract: Disclosed herein are derivatives of Streptolysin O produced by recombinant DNA techniques. In an embodiment, the derivative is soluble upon expression and has a specific hemolytic activity of about 3.6 x 10 hemolytic units per milligram.

Abstract translation: 本文公开了通过重组DNA技术产生的链球菌溶血素O的衍生物。 在一个实施方案中，衍生物在表达时可溶，并且具有约3.6×10 4溶血单位/毫克的特异性溶血活性。