公开(公告)号：WO1993010258A1

公开(公告)日：1993-05-27

申请号：PCT/US1992009873

申请日：1992-11-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  HSIEH, You-Zung ,  CHEN, Fu-Tai, A. ,  STERNBERG, James, C. ,  KLIEN, Gerald ,  LIU, Cheng-Ming

IPC: C12Q01/00

CPC classification number: C12Q1/007 ,  G01N27/44747

Abstract: Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ('CZE') techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.

公开(公告)号：EP0572604A1

公开(公告)日：1993-12-08

申请号：EP92925261.0

申请日：1992-11-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： HSIEH, You-Zung ,  CHEN, Fu-Tai, A. ,  STERNBERG, James, C. ,  KLIEN, Gerald ,  LIU, Cheng-Ming

IPC: C12Q1 ,  G01N27

CPC classification number: C12Q1/007 ,  G01N27/44747

Abstract: Procédé d'analyse d'isoenzymes utilisant des techniques d'électrophorèse à zone capillaire ("CZE"). Un échantillon contenant une isoenzyme et un substrat pouvant être catalysé par ladite isoenzyme en un produit de réaction sont introduits dans une colonne capillaire comprenant un tampon. Idéalement, le tampon contient le substrat avant l'introduction de l'échantillon. On applique à la colonne des techniques de séparation CZE, afin de séparer les isoenzymes en des zones discrètes. A la fin de la séparation, le produit est rapidement généré par la conversion catalytique du substrat par les isoenzymes, et accumulé dans chaque zone discrète. Le produit est ensuite détecté. On peut dériver les informations sur la répartition relative des isoenzymes à partir de la distribution relative du produit.

公开(公告)号：EP0572604B1

公开(公告)日：1997-05-07

申请号：EP92925261.7

申请日：1992-11-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： HSIEH, You-Zung ,  CHEN, Fu-Tai, A. ,  STERNBERG, James, C. ,  KLIEN, Gerald ,  LIU, Cheng-Ming

IPC: C12Q1/00 ,  G01N27/447

CPC classification number: C12Q1/007 ,  G01N27/44747

Abstract: Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ('CZE') techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.