公开(公告)号：WO1994006939A1

公开(公告)日：1994-03-31

申请号：PCT/US1993008857

申请日：1993-09-20

Applicant: AMOCO CORPORATION

Inventor： AMOCO CORPORATION ,  FARRELL, Michael, P. ,  LIN-GOERKE, Juili, L.

IPC: C12Q01/68

CPC classification number: C12N9/127 ,  C12Q1/6848 ,  C12Q1/6867

Abstract: Disclosed herein is a composition comprising ribonuclease treated RNA or DNA dependent RNA polymerase, and the use of same in amplification methods. The treatment of the RNA or DNA dependent RNA polymerase with ribonuclease reduces or eliminates false positives which result from the presence of an endogenous or contaminating replicatable template species in the Q(beta) replicase enzyme preparation.

Abstract translation: 本文公开了包含核糖核酸酶处理的RNA或DNA依赖性RNA聚合酶的组合物，以及在扩增方法中的用途。 使用核糖核酸酶处理RNA或DNA依赖性RNA聚合酶可以减少或消除由Q（β）复制酶制剂中的内源性或污染性可重复模板物质的存在引起的假阳性。

公开(公告)号：WO1994006812A1

公开(公告)日：1994-03-31

申请号：PCT/US1993008559

申请日：1993-09-10

Applicant: AMOCO CORPORATION

Inventor： AMOCO CORPORATION ,  CRUICKSHANK, Kenneth, H. ,  BITTNER, michael, L.

IPC: C07H21/00

CPC classification number: C07H21/00 ,  C12Q1/6841 ,  C12Q2563/107

Abstract: The invention provides green fluorescent labeled nucleotides suitable for use in nucleic acid synthesis and particularly for use as DNA probes in chromosome identification. The green fluorescent labels are derived from green rhodamine dyes and the invention also comprises a method for production of the labelled nucleotides.

Abstract translation: 本发明提供绿色荧光标记的核苷酸，其适用于核酸合成，尤其用作染色体鉴定中的DNA探针。 绿色荧光标记衍生自绿色罗丹明染料，本发明还包括生产标记核苷酸的方法。

公开(公告)号：WO1993023899A1

公开(公告)日：1993-11-25

申请号：PCT/US1993004180

申请日：1993-05-03

Applicant: AMOCO CORPORATION

Inventor： AMOCO CORPORATION ,  TIDWELL, Steve, C.

IPC: H01S03/094

CPC classification number: H01S3/09415

Abstract: A method and apparatus for uniformly end-pumping a laser source or amplifier by reflecting pump radiation from the side surface of the lasing medium as it travels through the lasing medium.

Abstract translation: 一种用于在激光介质的行进通过激光介质时从激光介质的侧表面反射泵浦辐射来均匀地端射泵浦激光源或放大器的方法和装置。

公开(公告)号：WO1993016127A1

公开(公告)日：1993-08-19

申请号：PCT/US1993001085

申请日：1993-02-08

Applicant: AMOCO CORPORATION

Inventor： AMOCO CORPORATION ,  LEDUC, Edward, C.

IPC: C08J09/12

CPC classification number: C08J9/127 ,  C08J2203/14 ,  C08J2203/142 ,  C08J2325/00 ,  Y10S521/91

Abstract: The invention comprises a method for the production of insulating styrenic foams comprising use of a blowing agent comprising a C4-C5 alkane, CO2 and a halogenated ethane selected from the group consisting of 1,1,1-trifluoroethane, 1-chloro-1,1-difluoroethane and 1,1,1,2-tetrafluoroethane and mixtures thereof, wherein the halogenated ethane amount is less than 70.0 wt. % of the total blowing agent weight. The invention further comprises the styrenic foams made using the blowing agent composition and a paraffin wax. The method of the invention advantageously eliminates ethylchloride from styrenic foam production methods while limiting use of the halogenated ethane to minimum amounts suitable to produce foams with good insulative properties.

公开(公告)号：WO1993010265A1

公开(公告)日：1993-05-27

申请号：PCT/US1992009889

申请日：1992-11-16

Applicant: AMOCO CORPORATION

Inventor： AMOCO CORPORATION ,  MCKENZIE, R., Scott

IPC: C12Q01/68

CPC classification number: C12Q1/6832 ,  Y02A50/451

Abstract: A method for enhancing the hybridization signal of a nucleic acid hybridization assay for the DNA or RNA of a target organism in a sample by adding the sample to a growth medium under conditions sufficient to allow any target organism in the sample to propagate, propagating the organism, if any, in the medium, for a time sufficient to allow the number of target organisms to reach a predetermined titer, removing trace minerals from the medium, adding a nucleic acid probe to the medium under stringency conditions sufficient to allow the probe to preferentially hybridize with the desired target organism, if any, to form hybridization products that emit an enhanced signal, and assaying the medium to detect the enhanced signal. The method is especially suited for use with RV medium in assays for Salmonella.

Abstract translation: 一种用于增强样品中靶生物的DNA或RNA的核酸杂交测定的杂交信号的方法，其通过在足以允许样品中的任何目标生物体传播，传播生物体的条件下将样品加入生长培养基中 （如果有的话）在培养基中，足以允许目标生物体的数量达到预定滴度的时间，从培养基中除去痕量矿物质，在足以允许探针优先的严格条件下向培养基中加入核酸探针 与期望的靶生物杂交（如果有的话）以形成发射增强信号的杂交产物，并测定培养基以检测增强的信号。 该方法特别适用于沙门氏菌测定中的RV培养基。

公开(公告)号：WO1993008151A1

公开(公告)日：1993-04-29

申请号：PCT/US1992008974

申请日：1992-10-14

Applicant: AMOCO CORPORATION

Inventor： AMOCO CORPORATION ,  HARPER, Jon, Jay ,  KUHLMANN, George, Edward ,  LARSON, Keith, Donald ,  McMAHON, Rosemary, Fischer ,  SANCHEZ, Paul, Anthony

IPC: C07C63/38

CPC classification number: C07C51/265 ,  C07C63/38

Abstract: Provided is a continuous process for producing 2,6-naphthalenedicarboxylic acid by the liquid phase oxidation of 2,6-dimethylnaphthalene comprising continuously adding to a reaction zone the oxidation reaction components comprising 2,6-dimethylnaphthalene, a source of molecular oxygen, a solvent comprising an aliphatic monocarboxylic acid, and a catalyst comprising cobalt, manganese and bromine components, wherein the atom ratio of manganese to cobalt is about 5:1 to about 0.3:1, the total of cobalt and manganese is at least about 0.40 weight percent based on the weight of solvent, and maintaining the contents of the reaction zone at a temperature of about 370 °F to about 420 °F and at a pressure sufficient to maintain at least a portion of the monocarboxylic acid in the liquid phase thereby oxidizing the 2,6-dimethylnaphthalene to 2,6-naphthalenedicarboxylic acid. By operating according to the process of this invention, 2,6-naphthalenedicarboxylic acid can be continuously produced in high yield and with low levels of impurities.

公开(公告)号：WO1993006246A1

公开(公告)日：1993-04-01

申请号：PCT/US1992008029

申请日：1992-09-21

Applicant: AMOCO CORPORATION

Inventor： AMOCO CORPORATION ,  PROROK, Clarissa, Frances ,  STOLS, Lucy, M. ,  BITTNER, Michael, Louis ,  CRUICKSHANK, Kenneth, Alexander

IPC: C12Q01/68

CPC classification number: C12N15/1093

Abstract: Techniques for producing cloned DNA sequences are provided which sequences are complementary to DNA occurring in one selected region of one chromosome of a multi-chromosomal genome, such as the human genome. Such cloned DNA sequences can be labeled and formed into probes by conventional procedures, there are provided methods for making probe compositions which comprise mixed DNA segments derived from such a DNA sequence. An improved DNA sequence transamination procedure is provided utilizing trifluoroacetate chaotrope anions. With high concentrations of low complexity DNA, high levels of transamination are thereby achieved. These segments are covalently bound to fluorophore groups through linking groups that are transaminated preferably chaotropically into the segments.

Abstract translation: 提供了用于产生克隆DNA序列的技术，其中哪些序列与多染色体基因组（例如人类基因组）的一个染色体的一个选定区域中存在的DNA互补。 可以通过常规方法将这种克隆的DNA序列标记并形成探针，提供了制备包含衍生自这种DNA序列的混合DNA片段的探针组合物的方法。 利用三氟乙酸离液剂阴离子提供了改进的DNA序列转氨法。 由于具有高浓度的低复杂度DNA，因此实现了高水平的转氨酶。 这些链段通过交联基团共价结合到荧光团上，所述基团优选地被顺向地转化成片段。

公开(公告)号：WO1993004201A1

公开(公告)日：1993-03-04

申请号：PCT/US1992006821

申请日：1992-08-13

Applicant: AMOCO CORPORATION

Inventor： AMOCO CORPORATION ,  LIU, Jing ,  NIETUPSKI, Raymond, Mark ,  SHAH, Jyotsna, Sudhir

IPC: C12Q01/68

CPC classification number: C12Q1/689

Abstract: Disclosed are nucleic acid oligonucleotides which are substantially complementary to nucleic acids from Mycobacteria, and subgeneric classes thereof. More specifically, the oligonucleotides are complementary to ribosomal RNA (rRNA) and the DNA encoding rRNA (rDNA). Uses for such oligonucleotides include detection of Mycobacteria by hybridization and amplification of Mycobacterial nucleic acid by polymerase chain reaction.

Abstract translation: 公开了与分枝杆菌的核酸基本互补的核酸寡核苷酸及其亚类。 更具体地，寡核苷酸与核糖体RNA（rRNA）和编码rRNA（rDNA）的DNA互补。 用于这样的寡核苷酸包括通过聚合酶链反应杂交和扩增分枝杆菌核酸来检测分枝杆菌。

公开(公告)号：WO1992021645A1

公开(公告)日：1992-12-10

申请号：PCT/US1992004384

申请日：1992-05-21

Applicant: AMOCO CORPORATION

Inventor： AMOCO CORPORATION ,  HOLZHAUER, Juergen, Klaus ,  YOUNG, David, Allen ,  ZEITLIN, Martin, Allen ,  BEHRENDS, Raymond, Tony

IPC: C07C67/52

CPC classification number: C07C67/52 ,  C07C69/76

Abstract: A process for preparing purified dimethyl-2,6-naphthalenedicarboxylate by the esterification of 2,6-naphthalenedicarboxylic acid is disclosed and which process provides for the efficient removal of the mono methyl ester of 2,6-naphthalenedicarboxylic acid produced during the esterification reaction.

公开(公告)号：WO1992014698A1

公开(公告)日：1992-09-03

申请号：PCT/US1991000966

申请日：1991-02-13

Applicant: AMOCO CORPORATION

Inventor： AMOCO CORPORATION ,  ERNST, Andreas, Bernard ,  CASPARI, Gunter

IPC: C07C315/06

CPC classification number: C07C315/06 ,  C07C317/22

Abstract: Described herein is a purification process to make high isomeric purity, essentially colorless 4,4'-dihydroxydiphenyl sulfone from an impure isomeric mixture comprising 4,4'-dihydroxydiphenyl sulfone, 2,4'-dihydroxydiphenyl sulfone, and optionally triphenol disulfone, which combines one or more recrystallizations from water or water with a small amount of added base and decolorization with a carbonaceous adsorbent.

Abstract translation: 本文描述了一种从异构体纯度高的纯化方法，包括4,4'-二羟基二苯砜，2,4'-二羟基二苯基砜和任选的三酚二砜的不纯的异构体混合物的基本上无色的4,4'-二羟基二苯砜 用少量加入的碱进行一次或多次从水或水中重结晶，并用碳质吸附剂进行脱色。