公开(公告)号：WO1988000704A1

公开(公告)日：1988-01-28

申请号：PCT/US1987001609

申请日：1987-07-08

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ZAKOWSKI, Jack, J. ,  CUOMO, Vincent, A. ,  BLANKE, Gordon, C. ,  MEYER, Richard, C.

IPC: G01N35/00

CPC classification number: G01N35/025 ,  Y10T436/111666

Abstract: A method of operating a clinical analyzer wherein the analyzer includes a plurality of reaction locations (30) divided into two groups. Predetermined operations are performed on the first and second groups during corresponding processing cycles. A process that is common to both cycles is performed simultaneously for reaction locations (30) in the first and second groups during only one of the cycles. The other of the cycles can thus accommodate operations unique to particular tests that would not otherwise be available on the analyzer. The common process may be washing of adjacent reaction locations included in the first and second groups.

Abstract translation: 一种操作临床分析仪的方法，其中分析仪包括分成两组的多个反应位置（30）。 在相应的处理周期期间对第一和第二组执行预定的操作。 对于两个循环共同的过程在仅一个循环期间同时对第一组和第二组中的反应位置（30）进行。 因此，其他循环可以适应特定测试所独有的操作，否则分析仪将无法使用这些测试。 常见的方法可能是洗涤包括在第一和第二组中的相邻反应位置。

公开(公告)号：WO1986006004A1

公开(公告)日：1986-10-23

申请号：PCT/US1986000746

申请日：1986-04-07

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  HARRIS, Paul, C. ,  STONE, Linda, J.

IPC: B01L03/00

CPC classification number: B01L3/502

Abstract: A system and method of immunoassay, both including the use of a reaction capsule (14) having a hydrophobic membrane (22) which may be repeatedly wetted and rendered hydrophobic. A pressure differential across the membrane causes liquid flow therethrough to be initiated and the hydrophobic state is then achieved by flowing gas through the membrane. The system includes a turntable (12) supporting a plurality of reaction capsules (14) and eccentric (66) means for agitating the turntable and capsules. The turntable is rotated to position the capsules at various processing stations, including sample introduction (86), reagent introduction (98), wash (100), substrate introduction (130) and read stations (132). A single cylinder two-inlet valve (178) may be used, one inlet (190) connected to liquid (196) and a second inlet (192) connected to a gas source (202), to provide both liquid and gas flow through the membrane.

Abstract translation: 一种免疫测定的系统和方法，包括使用具有疏水膜（22）的反应胶囊（14），所述疏水膜可被反复润湿并变得疏水。 跨过膜的压差导致液体流过其中，并且然后通过使气体流过膜来实现疏水状态。 该系统包括支撑多个反应胶囊（14）的转台（12）和用于搅拌转盘和胶囊的偏心（66）装置。 转盘旋转以将胶囊定位在各种处理站，包括样品引入（86），试剂引入（98），洗涤（100），基底引入（130）和读取站（132）。 可以使用单缸双入口阀（178），连接到液体（196）的一个入口（190）和连接到气体源（202）的第二入口（192），以提供液体和气体流过 膜。

公开(公告)号：WO1985005568A1

公开(公告)日：1985-12-19

申请号：PCT/US1985001032

申请日：1985-05-31

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  SUTTON, John, H., III

IPC: B04B05/04

CPC classification number: B04B5/0421

Abstract: A centrifuge rotor (18) is presented having a pivotal mount (26) for a sample container (10) upwardly offset from the radial plane (A-A') of maximal strength of the rotor (18) permitting a smaller diameter rotor (18) container (10) assembly and reducing exposed surface area of the rotor (18) container assembly.

Abstract translation: 离心机转子（18）具有用于样品容器（10）的枢转安装件（26），该样品容器（10）向上偏离转子（18）的最大强度的径向平面（A-A'），允许较小直径的转子 ）容器（10）组件并减小转子（18）容器组件的暴露表面积。

公开(公告)号：WO1985005052A1

公开(公告)日：1985-11-21

申请号：PCT/US1985000441

申请日：1985-03-18

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  WEDEMEYER, Robert, Carl ,  GIEBELER, Robert, Henry, Jr.

IPC: B04B15/02

CPC classification number: F25B21/02 ,  B04B15/02

Abstract: A thermoelectric temperature control assembly (10) for transferring heat to or from a heat sink (30). A nonconducting substrate (40) is provided with a plurality of mounting openings (44) for receiving the mounting features of a plurality of respective thermoelectric devices (50), (52) and (54). Each mounting openings (44) is internally partitioned so as to form a pair of flexible tongues (48) by which the thermoelectric devices may be clamped to a heat sink (30) to assure a good thermal contact therewith.

公开(公告)号：WO1985002258A1

公开(公告)日：1985-05-23

申请号：PCT/US1984001888

申请日：1984-11-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  CRAGLE, Linda, Katherine ,  HARRIS, Paul, C. ,  LEE, Shih-Yun ,  TUNG, Ker, Kong ,  VODIAN, Morton, Alan

IPC: G01N33/53

CPC classification number: G01N33/54393 ,  G01N33/53 ,  G01N33/54306 ,  G01N33/577 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/818 ,  Y10S436/819

Abstract: A improved immunoassay for assaying an antigenic substance (Ag) in a fluid. The immunoassay is the type which comprises contacting the fluid with at least one first entity selected from a group consisting of an antibody (Ab) to the Ag, a soluble, labeled antibody (L-Aba) to the Ag, and an antibody (Abb) to the Ag bound to a solid support (SC). The immunoassay is characterized in that the fluid is contacted with at least one additional entity selected from a group consisting of at least one different type of soluble, labeled antibody (L-Abc) to the Ag, at least one different type of antibody (Abd) bound to a solid carrier (SC1), and at least one different type of antibody (Abe) to the Ag. The SC1 is selected from a group consisting of SC, at least one different solid carrier (SC2), and mixtures thereof (SC and SC2). Each type of L-Abc, Abd-SC1, and Abe has a lower average affinity constant (K) for Ag than each respective K of L-Aba, Abb-SC and Abe; and the additional entity is present in an amount sufficient to avoid a hook effect. Also, an improved reagent of the type comprising at least one first entity selected from a group consisting of L-Aba, Abb-SC, and Ab. The reagent is characterized in that it further comprises at least one additional entity selected from a group consisting of at least one different type of L-Abc and at least one different type Abd-SC1, and at least one different type of Abe. Ab, L-Aba, Abb-SC, L-Abc, Abd-SC1, and Abe are as defined above.

Abstract translation: 用于测定流体中的抗原物质（Ag）的改进的免疫测定。 免疫测定法是将流体与至少一种选自Ag抗体（Ab），Ag的可溶性标记抗体（L-Aba）和抗体（Abb ）到与固体支持物（SC）结合的Ag。 免疫测定的特征在于使流体与至少一种选自由至少一种不同类型的可溶性标记抗体（L-Abc）至Ag，至少一种不同类型的抗体（Abd）的另外的实体接触 ）与固体载体（SC1）结合，并且至少一种不同类型的抗体（Abe）与Ag结合。 SC1选自SC，至少一种不同的固体载体（SC2）及其混合物（SC和SC2）。 每种类型的L-Abc，Abd-SC1和Abe对于Ag的平均亲和常数（K）低于L-Aba，Abb-SC和Abe的各自的K; 并且额外的实体以足以避免钩效应的量存在。 此外，还包括一种包含至少一种选自由L-Aba，Abb-SC和Ab组成的组的第一实体的改良试剂。 该试剂的特征在于，其还包含至少一种选自至少一种不同类型的L-Abc和至少一种不同类型的Abd-SC1和至少一种不同类型的Abe的附加实体。 Ab，L-Aba，Abb-SC，L-Abc，Abd-SC1和Abe如上所定义。

公开(公告)号：WO1985001348A1

公开(公告)日：1985-03-28

申请号：PCT/US1984001506

申请日：1984-09-19

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  CHIPMAN, Russell, A. ,  OBREMSKI, Robert, J. ,  BROWN, Christopher, W.

IPC: G01N21/27

CPC classification number: G01N21/274 ,  G01J3/42

Abstract: A multicomponent quantitative analytical method and apparatus wherein the method includes the steps of and the apparatus is capable of performing steps of obtaining a plurality of calibration spectra, transforming the calibration spectra using a transform with orthogonal basis vectors, obtaining a calibration matrix relating the transform spectra to concentrations of analytes in the calibration samples, obtaining a spectrum for an unknown sample, transforming the unknown sample spectrum, and relating the transformed unknown sample spectrum to the calibration matrix to thereby determine the concentration of analytes in the unknown sample.

公开(公告)号：WO1985000708A1

公开(公告)日：1985-02-14

申请号：PCT/US1984001166

申请日：1984-07-23

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MEYER, Richard, C. ,  KAN, Peter

IPC: H02P08/00

CPC classification number: H02P8/165 ,  H02P8/32

Abstract: The method includes varying the width of the high level drive impulses of a bi-level drive system based on the desired speed to be achieved by the stepper motor. A microprocessor is used to vary the pulse width.

Abstract translation: 该方法包括基于由步进电机实现的期望速度来改变双级驱动系统的高电平驱动脉冲的宽度。 微处理器用于改变脉冲宽度。

公开(公告)号：WO1985000663A1

公开(公告)日：1985-02-14

申请号：PCT/US1983001147

申请日：1983-07-25

Applicant: BECKMAN INSTRUMENTS, INC. ,  TUNG, Ker-Kong ,  HUANG, John, Jen-Yin ,  LEE, Shih-Yun ,  CHEN, Jung-Shou

Inventor： BECKMAN INSTRUMENTS, INC.

IPC: G01N33/54

CPC classification number: G01N33/577 ,  G01N33/54306

Abstract: "Two-site" or "sandwich" immunometric assay techniques for determination of the presence and/or concentration of antigenic substances in fluids using a combination of monoclonal and polyclonal antibodies. One antibody selected from a group consisting of monoclonalx and polyclonal(1-x) antibodies is presented in a soluble labeled form; a second antibody selected from a group consisting of monoclonal(1-x) and polyclonalx is presented bound to a solid carrier; and x is 0 or 1. The present invention further encompasses a kit comprising in association: (a) a soluble first antibody to an antigenic substance present in a fluid to be assayed, the first antibody being labeled and being selected from a group consisting of monoclonalx and polyclonal(1-x) antibodies; and (b) a second antibody to the antigenic substance, the second antibody being bound to a solid carrier and being selected from a group consisting of monoclonal(1-x) and polyclonalx antibodies; wherein x is 0 or 1.

Abstract translation: 使用单克隆抗体和多克隆抗体的组合来测定流体中抗原物质的存在和/或浓度的“双位点”或“三明治”免疫测定技术。 选自单克隆抗体和多克隆（1-x）抗体的一种抗体以可溶性标记形式存在; 选自由单克隆（1-x）和多克隆组成的组的第二抗体结合到固体载体上; 本发明还包括试剂盒，其包括：（a）对存在于待测定流体中的抗原物质的可溶性第一抗体，所述第一抗体标记并选自由以下组成的组： 单克隆抗体和多克隆（1-x）抗体; 和（b）抗原物质的第二抗体，所述第二抗体结合固体载体并选自单克隆（1-x）和多克隆抗体; 其中x为0或1。

公开(公告)号：WO1985000424A1

公开(公告)日：1985-01-31

申请号：PCT/US1984001029

申请日：1984-06-29

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  ZYSMAN, Lawrence, J. ,  GREENE, Malbone, W.

IPC: G01K07/24

CPC classification number: G01K7/20 ,  G01K7/24 ,  G01K15/00

Abstract: A system and method for indicating temperature of chemicals in a reaction vessel is provided by a container (10) holding a thermally efficient fluid (20) which has an identical configuration as the vessel and is positioned in the same position as the vessel. A temperature sensor (30) in thermal communication with the fluid provides an electrical signal indicating the temperature at which the reaction will take place.

Abstract translation: 用于指示反应容器中化学物质的温度的系统和方法由保持热容量流体（20）的容器（10）提供，该容器具有与容器相同的构造并且与容器相同的位置。 与流体热连通的温度传感器（30）提供指示反应将发生的温度的电信号。

公开(公告)号：WO1984000507A1

公开(公告)日：1984-02-16

申请号：PCT/US1983000773

申请日：1983-05-16

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  CHENG, David, W. ,  CHULAY, Steven, J.

IPC: B04B07/06

CPC classification number: B04B7/06 ,  B04B9/10 ,  B04B2007/065 ,  Y10T74/2109

Abstract: A breakaway base for use with an ultracentrifuge rotor wherein the base is designed to fracture when the rotor is above a specified speed so that the rotor will disengage from the drive spindle of the centrifuge. The base (16) has cutout areas (24, 26) to establish a high stress region (44, 46) that is designed to fracture above a specified rotor speed. The cutout areas (24, 26) are designed to receive lugs (52, 54) projecting from the bottom of the rotor. The cutout areas (24, 26) have slotted ledges (32, 34) through which fastening bolts connect to the lugs on the rotor. The fracture of the base in the high stress region in conjunction with the slotted ledges will result in the base fracturing into two parts which will cause rotor disengagement from the drive spindle of the centrifuge and prevent the rotor from spinning at a greater speed.

Abstract translation: 与超离心机转子一起使用的分离基座，其中，当转子高于指定速度时，基座被设计成断裂，使得转子将离开离心机的驱动主轴。 基座（16）具有切口区域（24,26），以形成高应力区域（44,46），该高应力区域设计成在指定转子速度以上断裂。 切口区域（24,26）设计成接收从转子底部伸出的凸耳（52,54）。 切口区域（24,26）具有开槽凸缘（32,34），紧固螺栓通过该凸缘连接到转子上的凸耳。 高应力区域的底部与开槽凸缘的断裂将导致基部压裂成两部分，这将导致转子脱离离心机的驱动主轴，并防止转子以更高的速度旋转。