公开(公告)号：EP2211219A3

公开(公告)日：2013-03-20

申请号：EP10151529.4

申请日：2010-01-25

Applicant: President and Fellows of Harvard College

Inventor： Xie, Xiaoliang Sunney ,  Freudinger, Christian ,  Min, Wei

IPC: G02B21/00 ,  G02B21/08 ,  G01H9/00 ,  G01J3/10 ,  G01J3/44 ,  G01N21/65

CPC classification number: G02B21/002 ,  G01J3/10 ,  G01J3/44 ,  G01J2003/1282 ,  G01N21/65 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2201/0675 ,  G02B21/082

Abstract: A microscopy imaging system is disclosed that includes a light source system, a spectral shaper, a modulator system, an optics system, an optical detector and a processor. The light source system is for providing a first train of pulses and a second train of pulses. The spectral shaper is for spectrally modifying an optical property of at least some frequency components of the broadband range of frequency components such that the broadband range of frequency components is shaped producing a shaped first train of pulses to specifically probe a spectral feature of interest from a sample, and to reduce information from features that are not of interest from the sample. The modulator system is for modulating a property of at least one of the shaped first train of pulses and the second train of pulses at a modulation frequency. The optical detector is for detecting an integrated intensity of substantially all optical frequency components of a train of pulses of interest transmitted or reflected through the common focal volume. The processor is for detecting a modulation at the modulation frequency of the integrated intensity of substantially all of the optical frequency components of the train of pulses of interest due to the non-lincar interaction of the shaped first train of pulses with the second train of pulses as modulated in the common focal volume, and for providing an output signal for a pixel of an image for the microscopy imaging system.

公开(公告)号：EP2366990A3

公开(公告)日：2012-10-10

申请号：EP11158204.5

申请日：2011-03-15

Applicant: Leica Microsystems CMS GmbH

Inventor： Knebel, Werner

IPC: G01N21/64 ,  G02B21/00

CPC classification number: G01N21/6408 ,  G01N21/6458 ,  G01N2201/0675 ,  G02B21/0076

Abstract: Zur Durchführung einer Fluoreszenz-Korrelations-Spektroskopie mit einem Fluoreszenz-Mikroskop (29) wird ein Bild einer Probe (40) aufgenommen. Anhand des Bildes wird mindestens ein zu untersuchender Beleuchtungsbereich (43) der Probe (40) ausgewählt. Zu jedem ausgewählten Beleuchtungsbereich (43) wird mindestens ein Beleuchtungsfokus (42) eines Beleuchtungslichtstrahls (24) in dem entsprechenden Beleuchtungsbereich (43) der Probe (40) erzeugt, wodurch fluoreszierende Farbstoffpartikel, die mit Probenmolekülen der Probe (40) gekoppelt sind, innerhalb des entsprechenden Beleuchtungsbereichs (43) zum Fluoreszieren angeregt werden. Das von den Farbstoffpartikeln emittierte Fluoreszenzlicht (44) wird detektiert und abhängig von dem detektierten Fluoreszenzlicht (44) wird mindestens ein Diffusionskoeffizient ermittelt, der repräsentativ für eine Diffusionsfähigkeit der fluoreszierenden Farbstoffpartikel und der mit den Farbstoffpartikeln gekoppelten Probenmolekülen ist.

公开(公告)号：EP2366990A2

公开(公告)日：2011-09-21

申请号：EP11158204.5

申请日：2011-03-15

Applicant: Leica Microsystems CMS GmbH

Inventor： Knebel, Werner, Dr.

IPC: G01N21/64 ,  G02B21/00

CPC classification number: G01N21/6408 ,  G01N21/6458 ,  G01N2201/0675 ,  G02B21/0076

Abstract: Zur Durchführung einer Fluoreszenz-Korrelations-Spektroskopie mit einem Fluoreszenz-Mikroskop (29) wird ein Bild einer Probe (40) aufgenommen. Anhand des Bildes wird mindestens ein zu untersuchender Beleuchtungsbereich (43) der Probe (40) ausgewählt. Zu jedem ausgewählten Beleuchtungsbereich (43) wird mindestens ein Beleuchtungsfokus (42) eines Beleuchtungslichtstrahls (24) in dem entsprechenden Beleuchtungsbereich (43) der Probe (40) erzeugt, wodurch fluoreszierende Farbstoffpartikel, die mit Probenmolekülen der Probe (40) gekoppelt sind, innerhalb des entsprechenden Beleuchtungsbereichs (43) zum Fluoreszieren angeregt werden. Das von den Farbstoffpartikeln emittierte Fluoreszenzlicht (44) wird detektiert und abhängig von dem detektierten Fluoreszenzlicht (44) wird mindestens ein Diffusionskoeffizient ermittelt, der repräsentativ für eine Diffusionsfähigkeit der fluoreszierenden Farbstoffpartikel und der mit den Farbstoffpartikeln gekoppelten Probenmolekülen ist.

Abstract translation: 该方法包括产生照明光束（24）并选择样品（40）的照明区域。 照明光束被分成三个部分光束（30）。 使用荧光显微镜（20）的显微镜光学系统将部分光束聚焦到照明区域上，以激发照明区域中的荧光染料颗粒发荧光。 检测由染料颗粒发射的荧光（44）。 基于荧光来确定代表各荧光染料颗粒的扩散性的扩散系数。 还包括用于执行荧光相关光谱的装置的独立权利要求，其包括荧光显微镜和完全耗尽的硅检测器。

公开(公告)号：EP2211219A2

公开(公告)日：2010-07-28

申请号：EP10151529.4

申请日：2010-01-25

Applicant: President and Fellows of Harvard College

Inventor： Xie, Xiaoliang Sunney ,  Freudinger, Christian ,  Min, Wei

IPC: G02B21/00 ,  G02B21/08 ,  G01H9/00

CPC classification number: G02B21/002 ,  G01J3/10 ,  G01J3/44 ,  G01J2003/1282 ,  G01N21/65 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2201/0675 ,  G02B21/082

Abstract: A microscopy imaging system is disclosed that includes a light source system, a spectral shaper, a modulator system, an optics system, an optical detector and a processor. The light source system is for providing a first train of pulses and a second train of pulses. The spectral shaper is for spectrally modifying an optical property of at least some frequency components of the broadband range of frequency components such that the broadband range of frequency components is shaped producing a shaped first train of pulses to specifically probe a spectral feature of interest from a sample, and to reduce information from features that are not of interest from the sample. The modulator system is for modulating a property of at least one of the shaped first train of pulses and the second train of pulses at a modulation frequency. The optical detector is for detecting an integrated intensity of substantially all optical frequency components of a train of pulses of interest transmitted or reflected through the common focal volume. The processor is for detecting a modulation at the modulation frequency of the integrated intensity of substantially all of the optical frequency components of the train of pulses of interest due to the non-lincar interaction of the shaped first train of pulses with the second train of pulses as modulated in the common focal volume, and for providing an output signal for a pixel of an image for the microscopy imaging system.

Abstract translation: 公开了一种显微镜成像系统，其包括光源系统，光谱整形器，调制器系统，光学系统，光学检测器和处理器。 光源系统用于提供第一列脉冲和第二脉冲串。 光谱整形器用于对频率分量的宽带范围的至少一些频率分量的光学特性进行光谱修改，使得频率分量的宽带范围成形，产生成形的第一脉冲串，从而特别地探测来自 样本，并从样本中不感兴趣的特征中减少信息。 调制器系统用于以调制频率调制成形的第一脉冲序列和第二脉冲串中的至少一个的性质。 光学检测器用于检测通过公共焦点体积传输或反射的感兴趣的脉冲串的基本上所有的光学频率分量的积分强度。 处理器用于检测由于成形的第一脉冲序列与第二脉冲序列的非线性交互作用而导致的感兴趣的脉冲序列的基本上所有的光学频率分量的积分强度的调制频率的调制 在公共焦点体积中调制，并且为显微成像系统的图像的像素提供输出信号。

公开(公告)号：EP2150805A1

公开(公告)日：2010-02-10

申请号：EP08760262.9

申请日：2008-05-30

Applicant: The European Community, represented by the European Commission

Inventor： BEDNARKIEWICZ, Arthur ,  WHELAN, Maurice

IPC: G01N21/64 ,  G01J3/44 ,  G02B21/00

CPC classification number: G01N21/6408 ,  G01N21/6458 ,  G01N2021/6423 ,  G01N2201/0675 ,  G02B21/16 ,  G02B21/365

Abstract: Method of fluorescence imaging comprising: a) illuminating a sample to excite its fluorescence and acquiring an image thereof; b) based on spectral and spatial information from the sample's fluorescence image, segmenting the image into regions of similar spectral properties; c) for each image segment, exciting the fluorescence of the corresponding sample region, and detecting the corresponding fluorescence; d) based on modelling, determining expected fluorescence parameters from the fluorescence signals detected for each region; e) scanning the sample and determining final fluorescence parameters based on said expected fluorescence parameters from step d).

公开(公告)号：EP1548481A1

公开(公告)日：2005-06-29

申请号：EP03808886.0

申请日：2003-09-18

Applicant: Japan Science and Technology Agency

Inventor： HAYASHI, Terutake ,  MAEKAWA, Katsuhiro ,  SHIBATA, Takayuki

IPC: G02B21/00 ,  G02B21/06 ,  G01N37/00 ,  C12N15/09

CPC classification number: G01N21/6458 ,  G01N21/21 ,  G01N21/6445 ,  G01N21/6452 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/0675 ,  G02B21/0024 ,  G02B21/0068 ,  G02B21/0076

Abstract: The present invention relates to a confocal microscope and the measuring methods of fluorescence and the polarized light using the same, and said confocal microscope is provided with the inlet optical part (10, 10') to let the polarized light from an illuminating light source (11) onto an object to be observed (2) via a matrix type liquid crystal device (22) provided with a microlens array (21) on its top part, and an objective lens (23), the light detecting part (30, 30') to detect the reflected or the fluorescent light from the object to be observed, and the liquid crystal control subpart (52) to control a liquid crystal device (22), and it transmits the light passing through said microlens array (21) from each microlens to each pixel (22a) of the liquid crystal device (22), and makes a plurality of foci (24) on the object to be observed (2) by the objective lens (23), as well as controls polarization directions of the lights transmitted through each pixel of the liquid crystal device (22) using the liquid crystal control subpart (52) so that they are made mutually orthogonal.

Abstract translation: 本发明涉及共焦显微镜以及使用其的荧光和偏振光的测量方法，并且所述共聚焦显微镜设置有入射光学部件（10,10'），以使来自照明光源的偏振光 11）通过在其顶部上设置有微透镜阵列（21）的矩阵型液晶装置（22）和物镜（23）到待观察的物体（2）上，所述光检测部分（30,30） '）来检测来自被观察物体的反射光或荧光，以及液晶控制部（52）来控制液晶装置（22），将透过所述微透镜阵列（21）的光透过 对液晶装置（22）的每个像素（22a）进行微透镜，并且通过物镜（23）在被观察物体（2）上形成多个焦点（24），并且控制偏振方向 光通过液晶装置的每个像素传输 e（22）使用液晶控制子部（52），使得它们相互正交。

公开(公告)号：EP0548830B1

公开(公告)日：1997-03-12

申请号：EP92121566.1

申请日：1992-12-18

Applicant: TEXAS INSTRUMENTS INCORPORATED

Inventor： Stafford, Ronald E.

IPC: G01J3/28

CPC classification number: G01J3/28 ,  G01J3/021 ,  G01J3/0229 ,  G01J2003/2866 ,  G01N2201/0675

公开(公告)号：EP0548830A1

公开(公告)日：1993-06-30

申请号：EP92121566.1

申请日：1992-12-18

Applicant: TEXAS INSTRUMENTS INCORPORATED

Inventor： Stafford, Ronald E.

IPC: G01J3/28

CPC classification number: G01J3/28 ,  G01J3/021 ,  G01J3/0229 ,  G01J2003/2866 ,  G01N2201/0675

Abstract: A SLM spectrometer is provided that employs an entrance slit (40) or a collimator (42) to provide parallel rays of radiation to a prism (44) which disperses the incident radiation into an associated wavelength spectrum. The resulting spectrum from the prism (44) is incident upon a spatial light modulator (SLM) (46), such as a deformable mirror device (DMD). By selectively activating (or deactivating) a small portion of the surface of the SLM, i.e. a cell on the SLM, it is possible to selectively reflect or transmit a portion of the spectrum incident upon the SLM onto a focusing device, such as a parabolic focusing mirror (48). The focusing device in turn focuses the portion of the spectrum reflected by the selected cells on the SLM to a sensor (50). The wavelength selected is a function of which row of cells are activated (or deactivated) in the SLM. The SLM spectrometer of the present invention may be used to analyze visible light and light that is near visible, such as the near infrared or ultraviolet regions. The output of the sensor or detector may be appropriately amplified and after appropriate calibration employed to determine the amount of energy in a particular wavelength or band of wavelengths.

Abstract translation: 提供SLM光谱仪，其使用入射狭缝（40）或准直器（42）向棱镜（44）提供平行射线，其将入射的辐射分散到相关的波长光谱中。 来自棱镜（44）的所得光谱入射到诸如可变形反射镜装置（DMD）的空间光调制器（SLM）（46）上。 通过选择性地激活（或去激活）SLM的表面的一小部分，即SLM上的单元，可以选择性地将入射到SLM上的频谱的一部分反射或发射到聚焦装置上，例如抛物线 聚焦镜（48）。 聚焦装置又将由SLM上选定的单元反射的光谱的一部分聚焦到传感器（50）上。 选择的波长是在SLM中激活（或停用）单元格行的功能。 本发明的SLM光谱仪可以用于分析近似可见光的可见光和近红外或紫外线区域的光。 传感器或检测器的输出可以被适当地放大，并且在适当的校准之后，用于确定特定波长或波段的能量的量。

公开(公告)号：EP2314202B1

公开(公告)日：2018-01-03

申请号：EP10187465.9

申请日：2010-10-13

Applicant: CANON KABUSHIKI KAISHA

Inventor： Hirose, Futoshi ,  Saito, Kenichi

IPC: A61B3/12 ,  A61B5/00 ,  G01N21/47 ,  G02B26/06 ,  G02F1/1335 ,  G02F1/1347

CPC classification number: G02B26/06 ,  A61B3/102 ,  A61B3/14 ,  G01N21/21 ,  G01N21/4795 ,  G01N2021/1787 ,  G01N2201/0675 ,  G02F2203/12 ,  G02F2203/18

Abstract: An adaptive optics apparatus includes light modulation means (159-1, 159-2) configured to modulate each of two polarization components of light at a position that is optically conjugate to an object (107), the light being emitted by a light source (101); and irradiation means configured to irradiate the object with light that is modulated by the light modulation means.

公开(公告)号：EP2384431B1

公开(公告)日：2017-11-29

申请号：EP09787366.5

申请日：2009-10-06

Applicant: Hemics B.V.

Inventor： VAN BEEK, Michael, C. ,  RENSEN, Wouter, H., J. ,  HARBERS, Rik

IPC: G01N21/47 ,  G01N21/49 ,  G01N21/59 ,  A61B5/00

CPC classification number: A61B5/0059 ,  A61B5/4528 ,  G01N21/4795 ,  G01N2201/0626 ,  G01N2201/0635 ,  G01N2201/0675 ,  G01N2201/124