公开(公告)号：EP3245491A1

公开(公告)日：2017-11-22

申请号：EP16703191.3

申请日：2016-01-11

Applicant: Verily Life Sciences LLC

Inventor： ACOSTA, Victor, Marcel ,  BACKER, Adam ,  PERREAULT, John, D. ,  PIPONI, Daniele, Paolo

IPC: G01J3/02 ,  G01J3/18 ,  G02F1/1335 ,  G02F1/29 ,  G02B5/18 ,  G02B26/08

CPC classification number: G02B21/0064 ,  G01J3/0205 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/0237 ,  G01J3/18 ,  G01J3/502 ,  G01J2003/1278 ,  G01J2003/468 ,  G01N21/255 ,  G01N21/27 ,  G01N2201/06113 ,  G01N2201/0675 ,  G02B5/1828 ,  G02B6/2931 ,  G02B21/06 ,  G02B21/361 ,  G02B26/0808 ,  G02F1/133553 ,  G02F1/23 ,  G02F1/292 ,  G06T7/11 ,  G06T7/90 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30004 ,  H04N5/2256 ,  H04N5/332

Abstract: An imaging system includes a light source configured to illuminate a target and a camera configured to image light responsively emitted from the target and reflected from a spatial light modulator (SLM). The imaging system is configured to generate high-resolution, hyperspectral images of the target. The SLM includes a refractive layer that is chromatically dispersive and that has a refractive index that is controllable. The refractive index of the refractive layer can be controlled to vary according to a gradient such that light reflected from the SLM is chromatically dispersed and spectrographs information about the target can be captured using the camera. Such a system could be operated confocally, e.g., by incorporating a micromirror device configured to control a spatial pattern of illumination of the target and to modulate the transmission of light from the target to the camera via the SLM according to a corresponding spatial pattern.

公开(公告)号：EP2480876A4

公开(公告)日：2017-10-25

申请号：EP10818176

申请日：2010-09-22

Applicant: POLYVALOR LTD PARTNERSHIP

Inventor： LESAGE FRÉDÉRIC ,  BÉLANGER SAMUEL ,  ABRAN MAXIME ,  PROVOST JEAN

IPC: G01N21/49 ,  A61B5/00 ,  A61B6/00 ,  G01N21/17 ,  G01N21/47 ,  G01N21/64 ,  G01N21/76

CPC classification number: G01N21/4795 ,  A61B5/0073 ,  A61B5/0091 ,  A61B5/4312 ,  G01N21/6456 ,  G01N21/763 ,  G01N2021/1787 ,  G01N2021/479 ,  G01N2201/0675

公开(公告)号：EP2997353A4

公开(公告)日：2016-12-28

申请号：EP14797836

申请日：2014-05-15

Applicant: THE ADMINISTRATORS OF THE TULANE EDUCATIONAL FUND

Inventor： BROWN JONATHON QUINCY

IPC: G01N21/64 ,  A61B5/00 ,  A61B10/02 ,  A61B90/00 ,  G01N1/30 ,  G02B21/06 ,  G02B21/16 ,  G02B21/36 ,  G06K9/52 ,  G06K9/62 ,  H04N5/225

CPC classification number: G02B21/16 ,  A61B5/0071 ,  A61B10/02 ,  A61B2090/3941 ,  G01N1/30 ,  G01N21/6458 ,  G01N2001/302 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/0635 ,  G01N2201/0675 ,  G01N2201/129 ,  G02B21/0076 ,  G02B21/365 ,  G02B21/367 ,  G06K9/52 ,  G06K9/6267 ,  H04N5/2256

Abstract: Systems and methods are provided for evaluating a fresh tissue sample, prepared as to fluoresce under illumination, during a medical procedure. A structured light source is configured to project a spatially patterned light beam onto the fresh tissue sample. An imaging system is configured to produce an image from fluorescence emitted from the illuminated fresh tissue sample. A system control is configured to provide a human-comprehensible clinically useful output associated with the medical procedure.

公开(公告)号：EP2801854A1

公开(公告)日：2014-11-12

申请号：EP13002490.4

申请日：2013-05-10

Applicant: Ruprecht-Karls-Universität Heidelberg

Inventor： Rossberger, Sabrina ,  Dithmar, Stefan Prof. Dr. ,  Best, Gerrit ,  Cremer, Christoph Prof. Dr.

IPC: G02B21/00 ,  G01N21/64 ,  G02B21/16 ,  G02B21/36 ,  G02B27/58

CPC classification number: G02B21/367 ,  G01N21/6445 ,  G01N21/6458 ,  G01N2201/0675 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/361 ,  G02B27/58

Abstract: The invention relates to a fluorescent microscope and a respective method for obtaining super-resolution images of a sample labelled with at least one type fluorescent label by combining localization microscopy and structured illumination microscopy. In an aspect, the fluorescent microscope comprises one or more light sources and an illumination system having a structured illumination path, in which a pattern generation system is positioned, for illuminating the sample with structured illumination light and a localization illumination path for illuminating the sample with localization illumination light. A switching mechanism is configured to switch between a first, a second and/or a third mode, wherein in the first mode at least a portion of the light emitted from the one or more light sources propagates through one of the illumination paths; in the second mode at least a portion of the light emitted from the one or more light sources propagates through the other one of the illumination paths; and in the third mode at least a portion of the light emitted from one or more of the light sources propagates through one illumination path while simultaneously at least another portion of the light emitted from one or more of the light sources propagates through the other illumination path. At least one image detector positioned in an optical detection path, configured to detect at least a portion of fluorescent light emitted from fluorescent molecules of the illuminated sample.
Another aspect concerns a method for obtaining super-resolution image data of a sample labeled with at least one type of fluorescent label comprising illuminating the sample with localization illumination light and with structured illumination light; detecting at least a portion of the fluorescent light emitted from the excited fluorescent molecules of the at least one fluorescent label, thereby obtaining at least one image of the illuminated sample; and processing the obtained at least one image of the sample image to obtain super-resolution image data.

Abstract translation: 本发明涉及的荧光显微镜和用于respectivement通过组合定位显微镜和结构照明显微镜获取用至少一种类型的荧光标记物的样品的超分辨率图像的方法。 看点到荧光显微镜包括一个或多个光源和照明系统，其具有结构化照明路径，其中，图案生成系统被定位时，用于照射具有结构化照明光的样品和用于与照射样本本地化照明路径 本地化的照明光。 一种开关机构被配置为将第一，第二和/或第三模式之间切换，worin在第一模式中的至少从所述一个或多个光源发出的光的一部分穿过的照明路径中的一个传播; 在第二模式中的至少从所述一个或多个光源发出的光的一部分传播通过照明路径中的另一个; 并且在第三模式中的至少从一个或多个光源发出的光的一部分传播通过一个照明路径，同时从一个或多个光源发出的光的同时至少另一部分传播通过其他照明路径 。在光学检测路径，被配置为检测至少荧光光从照射的样品的荧光分子发射的一部分定位在至少一个图像检测器。 另一个方面涉及一种用于获得用至少一种类型的荧光标记物包括利用照明光照明，并具有结构化照明光本地化样本的样本的超分辨率图像数据的方法; 至少检测所述荧光的光从所述至少一个荧光标记的激发的荧光分子，从而获得被照射的样品中的至少一个图像发射的的一部分; 和处理所述样本图像的获得的至少一个图像，以获得超分辨率图像数据。

公开(公告)号：EP2786123A1

公开(公告)日：2014-10-08

申请号：EP12821119.0

申请日：2012-11-30

Applicant: The University of Nottingham

Inventor： SHARPLES, Stephen ,  LIGHT, Roger

IPC: G01N21/17 ,  G01H9/00 ,  H01L27/146

CPC classification number: G01N21/1702 ,  G01H9/00 ,  G01N2201/0612 ,  G01N2201/0675 ,  H01L27/14609

Abstract: An optical detector comprises a plurality of pixels, each pixel comprising a photodiode operable to detect light incident on that pixel and to generate a signal indicative of an intensity of that light. The plurality of pixels comprises a plurality of pixel pairs, and for each pixel pair, in a configuration mode, the detector is arranged to compare the signal generated by a first pixel of the pair with the signal generated by a second pixel of the pair. A method of optical detection is also described, as is a system incorporating the described optical detector.

Abstract translation: 光学检测器包括多个像素，每个像素包括光电二极管，其可操作以检测入射在该像素上的光并产生指示该光强度的信号。 多个像素包括多个像素对，并且对于每个像素对，在配置模式下，检测器被布置为将由该对的第一像素生成的信号与由该对的第二像素生成的信号进行比较。 还描述了光学检测的方法，以及包含所描述的光学检测器的系统。

公开(公告)号：EP2314202A1

公开(公告)日：2011-04-27

申请号：EP10187465.9

申请日：2010-10-13

Applicant: CANON KABUSHIKI KAISHA

Inventor： Hirose, Futoshi ,  Saito, Kenichi

IPC: A61B3/12 ,  A61B5/00 ,  G01N21/47 ,  G02B26/06 ,  G02F1/1335 ,  G02F1/1347

CPC classification number: G02B26/06 ,  A61B3/102 ,  A61B3/14 ,  G01N21/21 ,  G01N21/4795 ,  G01N2021/1787 ,  G01N2201/0675 ,  G02F2203/12 ,  G02F2203/18

Abstract: An adaptive optics apparatus includes light modulation means (159-1, 159-2) configured to modulate each of two polarization components of light at a position that is optically conjugate to an object (107), the light being emitted by a light source (101); and irradiation means configured to irradiate the object with light that is modulated by the light modulation means.

Abstract translation: 一种自适应光学装置包括：光调制装置（159-1,159-2），被配置为调制与物体（107）光学共轭的位置处的光的两个偏振分量中的每一个，光被光源 101）; 以及被配置为用由所述光调制装置调制的光照射所述物体的照射装置。

公开(公告)号：EP2225599A2

公开(公告)日：2010-09-08

申请号：EP08852322.0

申请日：2008-11-21

Applicant: Light 4 Tech Firenze S.r.l.

Inventor： GALIMBERTI, Massimo ,  PAVONE, Francesco Saverio

IPC: G02B21/00 ,  G01N21/64

CPC classification number: G01N21/6458 ,  G01J3/02 ,  G01J3/0205 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/14 ,  G01J3/4406 ,  G01J2003/1286 ,  G01N21/645 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2201/0675

Abstract: A device to illuminate a object, to excite its fluorescence light emission, and detect the emitted fluorescence spectrum, comprising: at least one illumination system (13), adapted to receive light from a light source (11), to select at least one wavelength bands of light spectrum of the source (11), to illuminate a object (15) with light filtered in that way (14); and a detection system (17), adapted to detect fluorescence light (16) emitted by the object (15), to select at least one wavelength bands of fluorescence, light spectrum (16), to record the spectrum of the filtered light; characterized in that said illumination system (13) comprises: at least one first dispersive element (41), at least one focusing optics (43), at least one spatial fitter of excitation (44), at least one collimating optics (45) and at least one second dispersive element (47), wherein said detection system (17) comprises: at least one dispersive element (81), at least one focusing optics (83), at least one spatial filter of detection (84), at least one imaging optics (85) and at least one light detector (87).

Abstract translation: 一种照明物体，激发其荧光发射并检测所发射的荧光光谱的装置，包括：至少一个照明系统（13），适于接收来自光源（11）的光，以选择至少一个波长 （11）的光谱带，用以这种方式（14）过滤的光照亮物体（15）; 和检测系统（17），其适于检测由所述物体（15）发射的荧光光（16），以选择荧光光谱（16）的至少一个波段以记录所述滤光的光谱; 其特征在于所述照明系统（13）包括：至少一个第一色散元件（41），至少一个聚焦光学器件（43），至少一个激励空间拟合器（44），至少一个准直光学器件（45）和 至少一个第二色散元件（47），其中所述检测系统（17）包括：至少一个色散元件（81），至少一个聚焦光学器件（83），至少一个空间滤波器（84），至少一个 一个成像光学器件（85）和至少一个光检测器（87）。

公开(公告)号：EP1548481A4

公开(公告)日：2006-11-22

申请号：EP03808886

申请日：2003-09-18

Applicant: JAPAN SCIENCE & TECH AGENCY

Inventor： HAYASHI TERUTAKE ,  MAEKAWA KATSUHIRO ,  SHIBATA TAKAYUKI

IPC: G01N21/64 ,  C12N15/09 ,  G01N21/21 ,  G01N37/00 ,  G02B21/00 ,  G02B21/06

CPC classification number: G01N21/6458 ,  G01N21/21 ,  G01N21/6445 ,  G01N21/6452 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/0675 ,  G02B21/0024 ,  G02B21/0068 ,  G02B21/0076

Abstract: A cofocal microscope, and a fluorescence measuring method and a polarized light measuring method using it, the microscope comprising an incident optical system (10, 10’) for beaming a polarized light from a lighting light source (11) into an object of observation (2) via a matrix type liquid crystal element (22) having a micro-lens array (21) disposed thereon and an object lens (23), a detection optical system (30, 30’) for detecting a reflection light from the object of observation or a fluorescence, and a liquid crystal control unit (52) for controlling the liquid crystal element (22), wherein each micro-lens-related light passed through the micro-lens array (21) is allowed to pass through each pixel (22a) of the liquid crystal element (22) and then into an object lens (23) that forms a plurality of focal points (24) on the object of observation (2), and the polarizing directions of lights passing through respective pixels of the liquid crystal element (22) are controlled by the liquid crystal control unit (52) so as to be orthogonal to one another.

公开(公告)号：EP0587020A2

公开(公告)日：1994-03-16

申请号：EP93113897.8

申请日：1993-08-31

Applicant: TEXAS INSTRUMENTS INCORPORATED

Inventor： Sampsell, Jeffrey B. ,  Bienstock, Rachelle J.

IPC: G01N21/88 ,  G02B27/46 ,  G06K9/74 ,  G06E3/00

CPC classification number: G06K9/745 ,  G01N21/95623 ,  G01N2201/0675 ,  G02B27/46 ,  G06E3/005

Abstract: An optical correlation unit (10) for correlating the images of an inspection object and a reference object. The unit uses two phase modulating reflective spatial light modulators (12a, 12b). A first spatial light modulator (12a) receives electronic input in the form of image data representing the inspection object. It modulates incoming light (15) with this input and reflects the modulated output to a first Fourier transform lens (13). This lens provides the optical input to second spatial light modulator (12b), whose electronic input is transform data presenting the complex conjugate of the Fourier transform of the reference image. The electronic input modulates the optical input, resulting in the Fourier product of the two images, which is then transformed to provide correlation data.

Abstract translation: 一种用于使检查对象和参考对象的图像相关联的光学相关单元（10）。 该单元使用两个相位调制反射空间光调制器（12a，12b）。 第一空间光调制器（12a）以表示检查对象的图像数据的形式接收电子输入。 它用该输入调制入射光（15），并将调制的输出反射到第一傅里叶变换透镜（13）。 该透镜向第二空间光调制器（12b）提供光输入，其电子输入是呈现参考图像的傅里叶变换的复共轭的变换数据。 电子输入调制光学输入，产生两个图像的傅里叶积，然后将其转换为提供相关数据。

公开(公告)号：TW236008B

公开(公告)日：1994-12-11

申请号：TW082104266

申请日：1993-05-29

Applicant: 德州儀器公司

Inventor： 史納德

IPC: G02F

CPC classification number: G01J3/28 ,  G01J3/021 ,  G01J3/0229 ,  G01J2003/2866 ,  G01N2201/0675

Abstract: 本發明提供一種ＳＬＭ分光計，其採用一入射狹縫(40)或一準直器(42)，以提供平行輻射光線至一將入射之輻射彌散為關聯波長光譜之稜鏡(44)。自稜鏡(44)所獲得之光譜入射於一空間光調變器(spatial lighe modulator ，簡稱ＳＬＭ)，諸如一種可變形鏡裝置(de- formable mirror device,簡稱(DMD)。將ＳＬＭ表面之一小部份，亦即SLM上之一元件選擇性激勵（或去激勵），便可將入射於LSM之光譜之一部份選擇性反射或透射至一聚焦裝置，諸如一拋物柱面聚焦鏡(48)。聚焦裝置復將SLM上之選定元件所反射之光譜部份聚焦至一感測器(50)。所選擇之光譜為SLM中被激勵（或去激勵）之無論那一行元件之函數。本發明之SLM分光計可用以分析可見光及接近可見之光，諸如近紅外線或紫外線區。感測器或檢測器之輸出可予以適當放大，並且在適當校準後，用以確定在一特定波長或波長帶之能量。

Abstract in simplified Chinese: 本发明提供一种ＳＬＭ分光计，其采用一入射狭缝(40)或一准直器(42)，以提供平行辐射光线至一将入射之辐射弥散为关联波长光谱之棱镜(44)。自棱镜(44)所获得之光谱入射于一空间光调制器(spatial lighe modulator ，简称ＳＬＭ)，诸如一种可变形镜设备(de- formable mirror device,简称(DMD)。将ＳＬＭ表面之一小部份，亦即SLM上之一组件选择性激励（或去激励），便可将入射于LSM之光谱之一部份选择性反射或透射至一聚焦设备，诸如一抛物柱面聚焦镜(48)。聚焦设备复将SLM上之选定组件所反射之光谱部份聚焦至一传感器(50)。所选择之光谱为SLM中被激励（或去激励）之无论那一行组件之函数。本发明之SLM分光计可用以分析可见光及接近可见之光，诸如近红外线或紫外线区。传感器或检测器之输出可予以适当放大，并且在适当校准后，用以确定在一特定波长或波长带之能量。