公开(公告)号：US20100148073A1

公开(公告)日：2010-06-17

申请号：US12632187

申请日：2009-12-07

Applicant: Matthew P. Nelson ,  Patrick J. Treado

Inventor： Matthew P. Nelson ,  Patrick J. Treado

IPC: G01T1/28 ,  G01J1/58 ,  G01N21/65 ,  G01N21/64 ,  G01J3/44

CPC classification number: G01J3/2803 ,  G01J3/0221 ,  G01J3/44 ,  G01N21/474 ,  G01N21/55 ,  G01N21/645 ,  G01N21/6456 ,  G01N21/65 ,  G01N2021/6423 ,  G01N2021/6484 ,  G01N2201/0833 ,  G01N2201/0846 ,  G02B6/06

Abstract: The disclosure relates generally to methods and apparatus for using telescope optics and a fiber array spectral translator-based (“FAST”) spectroscopic system for improved imaging, spectral analysis, and interactive probing of a sample. In an embodiment, the confocality of a fiber array spectral translator-based spectroscopic system is improved through the use of structured illumination and/or structured collection of photons. User input may be received and acted upon to allow a user to interactively in real time and/or near real time view and analyze specific regions of the sample.

Abstract translation: 本公开一般涉及用于使用望远镜光学器件的方法和装置以及用于改进样品的成像，光谱分析和交互式探测的光纤阵列光谱转换器（“FAST”）光谱系统。 在一个实施例中，通过使用结构化照明和/或光子的结构化收集来提高基于光纤阵列光谱转换器的光谱系统的共焦度。 可以接收用户输入并执行操作以允许用户以实时和/或接近实时的方式进行交互式查看和分析样本的特定区域。

公开(公告)号：US20100118138A1

公开(公告)日：2010-05-13

申请号：US12575904

申请日：2009-10-08

Applicant: Youri N. Djachiachvili

Inventor： Youri N. Djachiachvili

IPC: H04N7/18 ,  G01N21/88

CPC classification number: G01N21/94 ,  G01N21/9018 ,  G01N21/95 ,  G01N2201/0833 ,  G02B6/06 ,  G02B2006/0098 ,  G06T7/0008 ,  G06T2207/30108

Abstract: An inspection station identifies defects such as artifacts (e.g., dust, hair, particles) in the sealing areas of sealed sterile packages. A multi-head optical scanner can include at least two fiber optic sensors each comprised of a bundle of optical fibers arranged into a linear face coupled to an image processing module and oriented towards a scanning area of sealed packages moving through a conveyance system. An image processing module can analyze input from the at least two fiber optic sensor arrangements to identify artifacts in the sealing areas of the sealed packages.

Abstract translation: 检查站在密封的无菌包装的密封区域中识别缺陷，例如伪像（例如，灰尘，毛发，颗粒）。 多头光学扫描器可以包括至少两个光纤传感器，每个光纤传感器由布置成耦合到图像处理模块的线性面的光纤束组成，并且朝向朝向通过传送系统移动的密封封装的扫描区域定向。 图像处理模块可以分析来自至少两个光纤传感器装置的输入，以识别密封包装的密封区域中的伪影。

公开(公告)号：US07527763B2

公开(公告)日：2009-05-05

申请号：US11174957

申请日：2005-07-05

Applicant: William Bedingham ,  Peter D. Ludowise ,  Barry W. Robole

Inventor： William Bedingham ,  Peter D. Ludowise ,  Barry W. Robole

IPC: G01N35/00 ,  G01N21/62

CPC classification number: F16K99/003 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/025 ,  B01L2300/021 ,  B01L2300/0806 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/004 ,  F16K2099/0084 ,  G01N21/0332 ,  G01N21/07 ,  G01N21/359 ,  G01N21/6428 ,  G01N21/6452 ,  G01N35/00069 ,  G01N35/1097 ,  G01N2021/0325 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2035/00267 ,  G01N2035/00415 ,  G01N2035/0474 ,  G01N2201/0407 ,  G01N2201/0415 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/0833 ,  Y10T436/11 ,  Y10T436/111666

Abstract: Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules that are optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. In addition, the device may control the flow of fluid in the disk by locating and selectively opening valves separating chambers by heating the valves with a laser.

Abstract translation: 描述了用于在实时PCR（聚合酶链式反应）中检测多种靶物种的技术。 例如，系统包括数据采集装置和耦合到数据采集装置的检测装置。 检测装置包括具有多个处理室的旋转盘，该多个处理室具有发射不同波长的荧光的多种物质。 该装置还包括多个可移除的光学模块，其被光学配置为激发物种并捕获由不同波长的物质发射的荧光。 耦合到多个可移除光学模块的光纤束将来自光学模块的荧光传送到单个检测器。 此外，该装置可以通过利用激光加热阀来定位和选择性地打开阀分离腔来控制盘中的流体流动。

公开(公告)号：US20080280331A1

公开(公告)日：2008-11-13

申请号：US12093132

申请日：2007-02-07

Applicant: Mark Davies ,  Tara Dalton

Inventor： Mark Davies ,  Tara Dalton

IPC: C12P19/34 ,  C12M1/02

CPC classification number: B01L7/525 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0838 ,  B01L2300/0867 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/185 ,  B01L2400/0487 ,  C12Q1/686 ,  G01N21/6428 ,  G01N2201/0826 ,  G01N2201/0833

Abstract: A thermal cycling device (3) device a number of fixed thermal zones (11, 12, 13) and a fixed conduit (10) passing through the thermal zones. A controller maintains each thermal zone including its section of conduit (10) at a constant temperature. A series of droplets flows through the conduit (10) so that each droplet is thermally cycled, and a detection system detects fluorescence from droplets at all of the thermal cycles. The conduit is in a single plane, and so a number of thermal cycling devices may be arranged together to achieve parallelism. The flow conduit comprises a channel (17) and a capillary tube (10) inserted into the channel. The detection system may perform scans along a direction to detect radiation from a plurality of cycles in a pass.

Abstract translation: 热循环装置（3）装置设有多个固定热区（11,12,13）和通过热区的固定导管（10）。 控制器将每个热区域保持在其恒定温度下的导管（10）部分。 一系列液滴流过导管（10），使得每个液滴热循环，并且检测系统在所有热循环中检测来自液滴的荧光。 导管处于单个平面中，因此可以将多个热循环装置布置在一起以实现平行。 流动管道包括插入通道中的通道（17）和毛细管（10）。 检测系统可以沿着一个方向执行扫描，以检测通过中多个周期的辐射。

公开(公告)号：US07365849B2

公开(公告)日：2008-04-29

申请号：US11249965

申请日：2005-10-12

Applicant: Wen Fa Yao ,  Chih Ming Wang ,  Yung Chuan Liu ,  Shih Yang Lo ,  Roger Lai ,  Kuang Pin Hsiung

Inventor： Wen Fa Yao ,  Chih Ming Wang ,  Yung Chuan Liu ,  Shih Yang Lo ,  Roger Lai ,  Kuang Pin Hsiung

IPC: G01J3/46

CPC classification number: G01N21/8483 ,  G01N21/274 ,  G01N21/78 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/8645 ,  G01N2021/8654 ,  G01N2201/0221 ,  G01N2201/0626 ,  G01N2201/0627 ,  G01N2201/064 ,  G01N2201/0833

Abstract: A portable, scanning and analyzing apparatus that uses an integrated scan probe for the scanning operation is described. The integrated scanning probe is formed with a light emitting diode array light source and a photodiode detector array. After a test sample finishes the reaction in test strip paper, a scanner device scans the test paper to collect the optical signals at variable, consecutive intervals along the scanning path to obtain the test signal accordingly. Then, the scanner device outputs the test signal for amplification. The amplified test signals are sent to an analog/digital converter such that the amplified test signals are converted into digital signals, which are then output to a computing unit for analyzing for subjective analytical results. The computing unit couples with the controller device, wherein the controller device controls a driver device that drives the scanner device to perform the scanning operation on the test paper.

Abstract translation: 描述了使用集成扫描探针进行扫描操作的便携式扫描和分析装置。 集成扫描探针由发光二极管阵列光源和光电二极管检测器阵列形成。 测试样品在测试条纸中完成反应后，扫描仪器将扫描测试纸，沿着扫描路径以可变的连续间隔收集光信号，从而获得相应的测试信号。 然后，扫描仪装置输出用于放大的测试信号。 放大的测试信号被发送到模拟/数字转换器，使得放大的测试信号被转换成数字信号，然后输出到计算单元以分析主观分析结果。 所述计算单元与所述控制器装置耦合，其中所述控制器装置控制驱动所述扫描仪装置以对所述测试纸进行扫描操作的驱动装置。

公开(公告)号：US20070276257A1

公开(公告)日：2007-11-29

申请号：US11381443

申请日：2006-05-03

Applicant: John HEANUE ,  Joseph Heanue ,  Brian Wilfley ,  Augustus Lowell

Inventor： John HEANUE ,  Joseph Heanue ,  Brian Wilfley ,  Augustus Lowell

IPC: A61B6/00

CPC classification number: A61B5/0059 ,  A61B5/415 ,  A61B5/418 ,  G01J3/02 ,  G01J3/0205 ,  G01J3/0218 ,  G01J3/10 ,  G01J3/2823 ,  G01J3/4406 ,  G01N21/6408 ,  G01N2201/0826 ,  G01N2201/0833

Abstract: A system and method for optical lymph node mapping. The system is useful for guiding sentinel lymph node biopsy surgeries. A contrast agent that includes a fluorescent dye is injected near the site of a malignancy. The contrast agent drains into the lymphatic system, collecting in a sentinel node or nodes. The system utilizes one or more low-power continuous-wave lasers or light-emitting diodes modulated with a digital code sequence to probe the tissue suspected of containing the sentinel node. When the light is incident near the sentinel node, it will excite fluorescence from the dye. A portion of the scattered fluorescent light is captured with one or more photo-detectors. A correlation of the photo-detector signal and the digital code sequence is calculated to produce an estimate of the distribution of flight times for photons traveling from a given source to a given detector. The flight time distributions are used along with the measured amplitudes to reconstruct a map of contrast agent location within the tissue.

Abstract translation: 一种用于光学淋巴结图谱的系统和方法。 该系统可用于引导前哨淋巴结活检。 包含荧光染料的造影剂在恶性肿瘤部位附近注射。 造影剂排入淋巴系统，收集在前哨节点或节点。 该系统利用一个或多个低功率连续波激光器或用数字代码序列调制的发光二极管来探测怀疑含有前哨节点的组织。 当光在哨兵节点附近入射时，它会激发染料的荧光。 一部分散射的荧光被一个或多个光电探测器捕获。 计算光检测器信号和数字码序列的相关性，以产生从给定源到给定检测器的光子的飞行时间分布的估计。 飞行时间分布与测量的振幅一起使用以重建组织内造影剂位置的图。

公开(公告)号：US20070201022A1

公开(公告)日：2007-08-30

申请号：US11679112

申请日：2007-02-26

Applicant: Jason H. Neiss ,  Patrick J. Treado ,  Robert Schweitzer

Inventor： Jason H. Neiss ,  Patrick J. Treado ,  Robert Schweitzer

IPC: G01J3/00

CPC classification number: G01J3/2803 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/0221 ,  G01J3/024 ,  G01J3/0291 ,  G01J3/0294 ,  G01J3/04 ,  G01J3/2823 ,  G01J3/44 ,  G01N21/65 ,  G01N2201/0833 ,  G01N2201/1293

Abstract: The disclosure relates generally to methods and apparatus for using a fiber array spectral translator-based (“FAST”) spectroscopic system for performing spectral unmixing of a mixture containing multiple polymorphs. In an embodiment, a first spectrum of a mixture containing polymorphs of a compound is obtained using a photon detector and a fiber array spectral translator having plural fibers. A set of second spectra is provided where each spectrum of the set of second spectra may be representative of a different polymorph of the compound. The first spectrum and the set of second spectra may be compared, and based on the comparison, the presence of one or more polymorphs in the mixture may be determined.

Abstract translation: 本公开总体上涉及使用用于执行含有多个多晶型物的混合物的光谱解混合的光纤阵列光谱转换器（“FAST”）光谱系统的方法和装置。 在一个实施方案中，使用具有多个纤维的光子检测器和光纤阵列光谱转换器获得含有化合物多晶型物的混合物的第一光谱。 提供了一组第二光谱，其中该组第二光谱的每个光谱可以代表该化合物的不同多晶型物。 可以比较第一光谱和第二光谱集，并且基于比较，可以确定混合物中一个或多个多晶型物的存在。

公开(公告)号：US20070188747A1

公开(公告)日：2007-08-16

申请号：US11674552

申请日：2007-02-13

Applicant: Matthew P. Nelson ,  Jason H. Neiss ,  Patrick J. Treado

Inventor： Matthew P. Nelson ,  Jason H. Neiss ,  Patrick J. Treado

IPC: G01D18/00 ,  G01J3/00 ,  G01C19/00

CPC classification number: G01J3/02 ,  G01J3/0218 ,  G01J3/0221 ,  G01J3/28 ,  G01J3/2803 ,  G01J3/2823 ,  G01N21/255 ,  G01N21/274 ,  G01N2201/0833

Abstract: The disclosure relates generally to methods and apparatus for spectral calibration of a spectroscopic system which includes a fiber array spectral translator. One embodiment relates to a method for obtaining a first image of a known substance using a photon detector and a fiber array spectral translator having plural fibers, wherein the first image comprises at least one pixel; providing a second image of the substance wherein the second image comprises at least one pixel; comparing the first image with the second image; and adjusting at least one pixel of the first image based on the comparison of images to thereby obtain an adjusted image.

Abstract translation: 本公开一般涉及包括光纤阵列光谱转换器的光谱系统的光谱校准的方法和装置。 一个实施例涉及一种使用光子检测器和具有多个光纤的光纤阵列光谱转换器获得已知物质的第一图像的方法，其中第一图像包括至少一个像素; 提供所述物质的第二图像，其中所述第二图像包括至少一个像素; 将第一图像与第二图像进行比较; 以及基于图像的比较来调整第一图像的至少一个像素，从而获得调整后的图像。

公开(公告)号：US20070166770A1

公开(公告)日：2007-07-19

申请号：US11333731

申请日：2006-01-17

Applicant: Huangpin Hsieh ,  Nicole Lazarus ,  Robert Krivacic ,  Douglas Curry ,  Richard Bruce

Inventor： Huangpin Hsieh ,  Nicole Lazarus ,  Robert Krivacic ,  Douglas Curry ,  Richard Bruce

IPC: G01N33/567

CPC classification number: G01N1/30 ,  G01N15/1468 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/56966 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2021/6484 ,  G01N2201/0833

Abstract: A method is provided for preparing a sample containing potential cells of interest and of using a laser of a laser based system for novel excitation and emission collection, and data usage including use of obtained data for direct and ratio based measurements. The prepared sample is configured to emit signals having spectral characteristics sufficient to permit filtering to differentiate and eliminate most false positives from true positives among acquired imaging events, in an imaging system employing a laser spot having a range of diameters from 1 to 20 μm or greater and which excites the fluorescence in a conventional or novel manner. These filtered events may be subsequently imaged and confirmed with another higher resolution device such as a fluorescent microscope in a short amount of time.

Abstract translation: 提供了一种制备包含潜在感兴趣的细胞的样品并使用基于激光的系统的激光进行新颖的激发和发射采集以及数据使用的方法，包括使用获得的数据进行直接和基于比率的测量。 所制备的样品被配置为发射具有足够的光谱特性的信号，以在使用具有1至20μm或更大直径范围的激光光斑的成像系统中进行滤光以区分并消除所获得的成像事件之间的真阳性的大多数假阳性 并以常规或新颖的方式激发荧光。 这些过滤的事件可以随后在较短的时间内用另一种较高分辨率的装置例如荧光显微镜进行成像和证实。

公开(公告)号：US07142296B2

公开(公告)日：2006-11-28

申请号：US10180647

申请日：2002-06-26

Applicant: Brian T. Cunningham ,  Peter Y. Li ,  Frank DeFilippi

Inventor： Brian T. Cunningham ,  Peter Y. Li ,  Frank DeFilippi

IPC: G01J3/28

CPC classification number: G01N21/253 ,  B01L3/5085 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0829 ,  G01N21/4788 ,  G01N21/7743 ,  G01N33/54373 ,  G01N2201/0635 ,  G01N2201/08 ,  G01N2201/0833 ,  G01N2201/0846 ,  G01N2201/1248 ,  G02B5/1809 ,  G02B5/1814 ,  G02B21/06

Abstract: Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An apparatus for detecting biochemical interactions occurring on the surface of a biosensor includes a light source. A first optical fiber is coupled to the light source and illuminates the biosensor. A second optical fiber detects a wavelength reflected from the biosensor. A spectrometer determines spectra of a reflected signal from the biosensor.